(Circular) (Six-base) MAPSORT of: pFN21AE1976.seq Check: 9891 from: 1 to: 6313 pFN21AE1976 6313 bp With 182 enzymes: SgfI * August 16, 2010 14:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4123 278 Size: 53 83 186 936 2587 2468 Fragments arranged by size: 2587 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3656 1937 Size: 1719 4594 Acc65I G'GTAC_C Cuts at: 3634 3634 Size: 6313 AclI AA'CG_TT Cuts at: 1563 4440 4813 1563 Size: 2877 373 3063 Fragments arranged by size: 3063 2877 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2259 3194 4376 5760 6028 1632 Size: 627 935 1182 1384 268 1917 Fragments arranged by size: 1917 1384 1182 935 627 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6116 AflIII A'CryG_T Cuts at: 1203 5212 6189 1203 Size: 4009 977 1327 Fragments arranged by size: 4009 1327 977 AgeI A'CCGG_T Cuts at: 2660 5144 2660 Size: 2484 3829 AhdI GACnn_n'nnGTC Cuts at: 5042 5042 Size: 6313 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6313 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2136 3239 1184 Size: 952 1103 4258 Fragments arranged by size: 4258 1103 952 AlwNI CAG_nnn'CTG Cuts at: 2449 2599 2770 3022 4056 5628 2449 Size: 150 171 252 1034 1572 3134 Fragments arranged by size: 3134 1572 1034 252 171 150 ApaI G_GGCC'C Cuts at: 3112 3121 3255 3112 Size: 9 134 6170 Fragments arranged by size: 6170 134 9 ApaLI G'TGCA_C Cuts at: 4370 5526 4370 Size: 1156 5157 ApoI r'AATT_y Cuts at: 1493 3123 3404 3622 3848 4000 6109 1493 Size: 1630 281 218 226 152 2109 1697 Fragments arranged by size: 2109 1697 1630 281 226 218 152 AseI AT'TA_AT Cuts at: 160 1032 4867 160 Size: 872 3835 1606 Fragments arranged by size: 3835 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6313 AvaI C'yCGr_G Cuts at: 1870 1943 3638 1870 Size: 73 1695 4545 Fragments arranged by size: 4545 1695 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3354 3387 3354 Size: 33 6280 BamHI G'GATC_C Cuts at: 3643 4010 3643 Size: 367 5946 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3276 3634 5089 618 Size: 325 217 627 1489 358 1455 1842 Fragments arranged by size: 1842 1489 1455 627 358 325 217 BanII G_rGCy'C Cuts at: 1391 2536 3009 3080 3112 3121 3198 3255 3632 Size: 1145 473 71 32 9 77 57 377 Cuts at: 3632 1391 Size: 4072 Fragments arranged by size: 4072 1145 473 377 77 71 57 32 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2314 928 Size: 971 415 4927 Fragments arranged by size: 4927 971 415 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4504 4538 1903 Size: 34 2567 34 3678 Fragments arranged by size: 3678 2567 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4205 5415 4205 Size: 1210 5103 BclI T'GATC_A Cuts at: 1548 2400 1548 Size: 852 5461 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2937 3116 4924 Size: 107 122 71 631 605 1265 179 1808 Cuts at: 4924 136 Size: 1525 Fragments arranged by size: 1808 1525 1265 631 605 179 122 107 71 BglII A'GATC_T Cuts at: 6308 6308 Size: 6313 BlpI GC'TnA_GC Cuts at: 2267 3730 2267 Size: 1463 4850 Bme1580I G_kGCm'C Cuts at: 1790 2867 2890 3112 3121 3255 4374 5530 1790 Size: 1077 23 222 9 134 1119 1156 2573 Fragments arranged by size: 2573 1156 1119 1077 222 134 23 9 BmgBI CAC'GTC Cuts at: 1318 2950 1318 Size: 1632 4681 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3353 4044 5002 Size: 416 53 83 399 24 1936 691 958 Cuts at: 5002 442 Size: 1753 Fragments arranged by size: 1936 1753 958 691 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6313 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3094 3094 Size: 6313 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2486 4973 1239 Size: 1247 2487 2579 Fragments arranged by size: 2579 2487 1247 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4438 5303 5601 4438 Size: 865 298 5150 Fragments arranged by size: 5150 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2365 2576 3573 4976 882 Size: 617 866 211 997 1403 2219 Fragments arranged by size: 2219 1403 997 866 617 211 BsaAI yAC'GTr Cuts at: 493 1204 2573 4104 493 Size: 711 1369 1531 2702 Fragments arranged by size: 2702 1531 1369 711 BsaBI GATnn'nnATC Cuts at: 6307 6307 Size: 6313 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4120 4502 5137 275 Size: 53 83 186 936 2587 382 635 1451 Fragments arranged by size: 2587 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2414 2660 4746 5144 5418 5565 5976 2414 Size: 246 2086 398 274 147 411 2751 Fragments arranged by size: 2751 2086 411 398 274 246 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2864 2894 3164 3194 1178 Size: 30 165 30 1461 30 270 30 4297 Fragments arranged by size: 4297 1461 270 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2200 2483 2564 2969 3086 1186 Size: 1014 283 81 405 117 4413 Fragments arranged by size: 4413 1014 405 283 117 81 BseYI C'CCAG_C Cuts at: 2624 2723 2742 2763 2779 3248 3259 5516 6012 Size: 99 19 21 16 469 11 2257 496 Cuts at: 6012 2624 Size: 2925 Fragments arranged by size: 2925 2257 496 469 99 21 19 16 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2875 3155 3535 3624 1163 Size: 573 1139 280 380 89 3852 Fragments arranged by size: 3852 1139 573 380 280 89 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3763 4524 5552 1540 Size: 275 186 1762 761 1028 2301 Fragments arranged by size: 2301 1762 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2536 2965 3080 3385 4374 4459 5530 2536 Size: 429 115 305 989 85 1071 3319 Fragments arranged by size: 3319 1071 989 429 305 115 85 BsmI GAATG_Cn' Cuts at: 3824 3917 3824 Size: 93 6220 BsmBI CGTCTCn'nnnn_ Cuts at: 2023 2223 2023 Size: 200 6113 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2536 2867 2890 2965 3009 3080 3112 Size: 399 746 331 23 75 44 71 32 Cuts at: 3112 3121 3198 3255 3385 3632 4374 4459 5530 Size: 9 77 57 130 247 742 85 1071 Cuts at: 5530 1391 Size: 2174 Fragments arranged by size: 2174 1071 746 742 399 331 247 130 85 77 75 71 57 44 32 23 9 BspEI T'CCGG_A Cuts at: 5976 5976 Size: 6313 BspHI T'CATG_A Cuts at: 2046 4202 5932 2046 Size: 2156 1730 2427 Fragments arranged by size: 2427 2156 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3668 844 Size: 2824 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2014 4200 1394 Size: 429 191 2186 3507 Fragments arranged by size: 3507 2186 429 191 BsrDI GCAATG_nn' Cuts at: 65 1248 2631 4808 4982 65 Size: 1183 1383 2177 174 1396 Fragments arranged by size: 2177 1396 1383 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2514 2660 4957 5144 1700 Size: 110 704 146 2297 187 2869 Fragments arranged by size: 2869 2297 704 187 146 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6313 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6313 BssSI C'ACGA_G Cuts at: 2058 2508 4373 5385 2058 Size: 450 1865 1012 2986 Fragments arranged by size: 2986 1865 1012 450 BstAPI GCAn_nnn'nTGC Cuts at: 794 2599 2862 794 Size: 1805 263 4245 Fragments arranged by size: 4245 1805 263 BstBI TT'CG_AA Cuts at: 5117 5117 Size: 6313 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6313 BstYI r'GATC_y Cuts at: 1972 3643 4010 4395 4412 5864 5950 5962 6308 Size: 1671 367 385 17 1452 86 12 346 Cuts at: 6308 1972 Size: 1977 Fragments arranged by size: 1977 1671 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2923 2923 Size: 6313 BtgI C'CryG_G Cuts at: 513 1065 2067 2300 2811 513 Size: 552 1002 233 511 4015 Fragments arranged by size: 4015 1002 552 511 233 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5574 Fragments arranged by size: 5574 683 56 BtsI GCAGTG_nn' Cuts at: 2438 3829 4623 4643 2438 Size: 1391 794 20 4108 Fragments arranged by size: 4108 1391 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6313 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2646 2681 529 Size: 35 2082 35 4161 Fragments arranged by size: 4161 2082 35 35 DraI TTT'AAA Cuts at: 3617 3617 Size: 6313 DraIII CAC_nnn'GTG Cuts at: 2832 3380 2832 Size: 548 5765 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5320 809 Size: 507 4004 1802 Fragments arranged by size: 4004 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2169 2451 2512 Size: 54 1425 50 275 18 339 282 61 Cuts at: 2512 2650 2786 3760 4649 6278 8 Size: 138 136 974 889 1629 43 Fragments arranged by size: 1629 1425 974 889 339 282 275 138 136 61 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3760 1537 Size: 275 1948 4090 Fragments arranged by size: 4090 1948 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2178 4243 1353 Size: 825 2065 3423 Fragments arranged by size: 3423 2065 825 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4900 5274 5420 387 Size: 1064 138 3311 374 146 1280 Fragments arranged by size: 3311 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2534 3078 2534 Size: 544 5769 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2259 2486 3194 4376 4973 5760 6028 Size: 393 627 227 708 1182 597 787 268 Cuts at: 6028 1239 Size: 1524 Fragments arranged by size: 1524 1182 787 708 627 597 393 268 227 EcoNI CCTnn'n_nnAGG Cuts at: 2468 3227 3284 2468 Size: 759 57 5497 Fragments arranged by size: 5497 759 57 EcoO109I rG'GnC_Cy Cuts at: 2293 2446 3108 3109 3117 3220 3252 3474 3495 Size: 153 662 1 8 103 32 222 21 Cuts at: 3495 2293 Size: 5111 Fragments arranged by size: 5111 662 222 153 103 32 21 8 1 EcoRI G'AATT_C Cuts at: 3622 4000 3622 Size: 378 5935 FspI TGC'GCA Cuts at: 4819 4819 Size: 6313 HaeII r_GCGC'y Cuts at: 1678 2617 2848 5460 1678 Size: 939 231 2612 2531 Fragments arranged by size: 2612 2531 939 231 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3164 3196 4954 4986 5028 Size: 32 135 32 1592 32 1758 32 42 Cuts at: 5028 5060 1373 Size: 32 2626 Fragments arranged by size: 2626 1758 1592 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3034 3657 3903 669 Size: 988 281 1096 623 246 3079 Fragments arranged by size: 3079 1096 988 623 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6313 HpaI GTT'AAC Cuts at: 3903 3903 Size: 6313 KpnI G_GTAC'C Cuts at: 3638 3638 Size: 6313 MfeI C'AATT_G Cuts at: 3912 3912 Size: 6313 MluI A'CGCG_T Cuts at: 6189 6189 Size: 6313 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3500 5427 5611 1305 Size: 2195 1927 184 2007 Fragments arranged by size: 2195 2007 1927 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2171 2453 2652 10 Size: 54 1425 343 339 282 199 3671 Fragments arranged by size: 3671 1425 343 339 282 199 54 MslI CAynn'nnrTG Cuts at: 518 1166 3431 4271 4630 4789 518 Size: 648 2265 840 359 159 2042 Fragments arranged by size: 2265 2042 840 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2624 2770 3419 3729 4406 5554 5799 1606 Size: 1018 146 649 310 677 1148 245 2120 Fragments arranged by size: 2120 1148 1018 677 649 310 245 146 NaeI GCC'GGC Cuts at: 2516 2516 Size: 6313 NcoI C'CATG_G Cuts at: 513 1065 2300 2811 513 Size: 552 1235 511 4015 Fragments arranged by size: 4015 1235 552 511 NdeI CA'TA_TG Cuts at: 387 2347 387 Size: 1960 4353 NgoMIV G'CCGG_C Cuts at: 2514 2514 Size: 6313 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6313 NotI GC'GGCC_GC Cuts at: 3760 3760 Size: 6313 NspI r_CATG'y Cuts at: 1132 1229 2123 2569 3671 5216 1132 Size: 97 894 446 1102 1545 2229 Fragments arranged by size: 2229 1545 1102 894 446 97 PasI CC'CwG_GG Cuts at: 1348 3009 3022 3066 1348 Size: 1661 13 44 4595 Fragments arranged by size: 4595 1661 44 13 PciI A'CATG_T Cuts at: 5212 5212 Size: 6313 PflMI CCAn_nnn'nTGG Cuts at: 1108 2770 2899 3022 1108 Size: 1662 129 123 4399 Fragments arranged by size: 4399 1662 129 123 PfoI T'CCnGG_A Cuts at: 3549 3549 Size: 6313 PmeI GTTT'AAAC Cuts at: 3617 3617 Size: 6313 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4382 4414 5895 5927 4382 Size: 32 1481 32 4768 Fragments arranged by size: 4768 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2293 2446 3474 3495 2293 Size: 153 1028 21 5111 Fragments arranged by size: 5111 1028 153 21 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6313 PsiI TTA'TAA Cuts at: 3883 3883 Size: 6313 PspOMI G'GGCC_C Cuts at: 3108 3117 3251 3108 Size: 9 134 6170 Fragments arranged by size: 6170 134 9 PstI C_TGCA'G Cuts at: 830 2379 2445 2526 2592 3231 3665 830 Size: 1549 66 81 66 639 434 3478 Fragments arranged by size: 3478 1549 639 434 81 66 66 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6313 PvuII CAG'CTG Cuts at: 2624 2770 3419 2624 Size: 146 649 5518 Fragments arranged by size: 5518 649 146 SacI G_AGCT'C Cuts at: 2536 3080 2536 Size: 544 5769 SalI G'TCGA_C Cuts at: 1936 3655 1936 Size: 1719 4594 SbfI CC_TGCA'GG Cuts at: 3665 3665 Size: 6313 ScaI AGT'ACT Cuts at: 4561 6273 4561 Size: 1712 4601 SexAI A'CCwGG_T Cuts at: 3376 3376 Size: 6313 SfcI C'TryA_G Cuts at: 826 1045 2375 2441 2522 2588 3227 3236 3661 Size: 219 1330 66 81 66 639 9 425 Cuts at: 3661 4796 5477 5668 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1330 1135 681 639 425 219 191 81 66 66 9 SfiI GGCCn_nnn'nGGCC Cuts at: 3116 3116 Size: 6313 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6313 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6313 SmaI CCC'GGG Cuts at: 1872 3640 1872 Size: 1768 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4417 5318 5580 820 Size: 197 926 2474 901 262 1553 Fragments arranged by size: 2474 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4104 493 Size: 3611 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6313 SphI G_CATG'C Cuts at: 1132 2123 3671 1132 Size: 991 1548 3774 Fragments arranged by size: 3774 1548 991 SspI AAT'ATT Cuts at: 5 52 4237 5 Size: 47 4185 2081 Fragments arranged by size: 4185 2081 47 StuI AGG'CCT Cuts at: 1517 2166 2201 1517 Size: 649 35 5629 Fragments arranged by size: 5629 649 35 StyI C'CwwG_G Cuts at: 513 1065 2300 2811 3112 3280 3342 3752 513 Size: 552 1235 511 301 168 62 410 3074 Fragments arranged by size: 3074 1235 552 511 410 301 168 62 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3555 4340 4357 4510 4695 5140 325 Size: 1255 1975 785 17 153 185 445 1498 Fragments arranged by size: 1975 1498 1255 785 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4559 6271 96 Size: 274 80 33 51 1443 2582 1712 138 Fragments arranged by size: 2582 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2864 2896 6273 6305 2864 Size: 32 3377 32 2872 Fragments arranged by size: 3377 2872 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6313 XbaI T'CTAG_A Cuts at: 3649 3649 Size: 6313 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2461 3091 3384 2461 Size: 630 293 5390 Fragments arranged by size: 5390 630 293 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6313 XmaI C'CCGG_G Cuts at: 1870 3638 1870 Size: 1768 4545 XmnI GAAnn'nnTTC Cuts at: 4442 4442 Size: 6313 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4121 276 Size: 53 83 186 936 2587 2468 Fragments arranged by size: 2587 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SfoI SrfI SwaI