(Linear) (Six-base) MAPSORT of: KIEE1976.seq Check: 9583 from: 1 to: 1608 KIEE1976.orf With 182 enzymes: SgfI * July 15, 2010 18:52 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 254 1189 1608 Size: 254 935 419 Fragments arranged by size: 935 419 254 AgeI A'CCGG_T Cuts at: 0 655 1608 Size: 655 953 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 131 1234 1608 Size: 131 1103 374 Fragments arranged by size: 1103 374 131 AlwNI CAG_nnn'CTG Cuts at: 0 444 594 765 1017 1608 Size: 444 150 171 252 591 Fragments arranged by size: 591 444 252 171 150 ApaI G_GGCC'C Cuts at: 0 1107 1116 1250 1608 Size: 1107 9 134 358 Fragments arranged by size: 1107 358 134 9 ApoI r'AATT_y Cuts at: 0 1118 1399 1608 Size: 1118 281 209 Fragments arranged by size: 1118 281 209 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1349 1382 1608 Size: 1349 33 226 Fragments arranged by size: 1349 226 33 BanI G'GyrC_C Cuts at: 0 1271 1608 Size: 1271 337 BanII G_rGCy'C Cuts at: 0 531 1004 1075 1107 1116 1193 1250 1608 Size: 531 473 71 32 9 77 57 358 Fragments arranged by size: 531 473 358 77 71 57 32 9 BbsI GAAGACnn'nnnn_ Cuts at: 0 309 1608 Size: 309 1299 BclI T'GATC_A Cuts at: 0 395 1608 Size: 395 1213 BglI GCCn_nnn'nGGC Cuts at: 0 932 1111 1608 Size: 932 179 497 Fragments arranged by size: 932 497 179 BlpI GC'TnA_GC Cuts at: 0 262 1608 Size: 262 1346 Bme1580I G_kGCm'C Cuts at: 0 862 885 1107 1116 1250 1608 Size: 862 23 222 9 134 358 Fragments arranged by size: 862 358 222 134 23 9 BmgBI CAC'GTC Cuts at: 0 945 1608 Size: 945 663 BmrI ACTGGGnnnn_n' Cuts at: 0 1348 1608 Size: 1348 260 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1089 1608 Size: 1089 519 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 481 1608 Size: 481 1127 BsaI GGTCTCn'nnnn_ Cuts at: 0 360 571 1568 1608 Size: 360 211 997 40 Fragments arranged by size: 997 360 211 40 BsaAI yAC'GTr Cuts at: 0 568 1608 Size: 568 1040 BsaWI w'CCGG_w Cuts at: 0 409 655 1608 Size: 409 246 953 Fragments arranged by size: 953 409 246 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 859 889 1159 1189 1608 Size: 859 30 270 30 419 Fragments arranged by size: 859 419 270 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 195 478 559 964 1081 1608 Size: 195 283 81 405 117 527 Fragments arranged by size: 527 405 283 195 117 81 BseYI C'CCAG_C Cuts at: 0 619 718 737 758 774 1243 1254 1608 Size: 619 99 19 21 16 469 11 354 Fragments arranged by size: 619 469 354 99 21 19 16 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 870 1150 1530 1608 Size: 870 280 380 78 Fragments arranged by size: 870 380 280 78 BsiHKAI G_wGCw'C Cuts at: 0 531 960 1075 1380 1608 Size: 531 429 115 305 228 Fragments arranged by size: 531 429 305 228 115 BsmBI CGTCTCn'nnnn_ Cuts at: 0 18 218 1608 Size: 18 200 1390 Fragments arranged by size: 1390 200 18 Bsp1286I G_dGCh'C Cuts at: 0 531 862 885 960 1004 1075 1107 1116 Size: 531 331 23 75 44 71 32 9 Cuts at: 1116 1193 1250 1380 1608 Size: 77 57 130 228 Fragments arranged by size: 531 331 228 130 77 75 71 57 44 32 23 9 BspHI T'CATG_A Cuts at: 0 41 1608 Size: 41 1567 BsrBI CCG'CTC Cuts at: 0 9 1608 Size: 9 1599 BsrDI GCAATG_nn' Cuts at: 0 626 1608 Size: 626 982 BsrFI r'CCGG_y Cuts at: 0 509 655 1608 Size: 509 146 953 Fragments arranged by size: 953 509 146 BssSI C'ACGA_G Cuts at: 0 53 503 1608 Size: 53 450 1105 Fragments arranged by size: 1105 450 53 BstAPI GCAn_nnn'nTGC Cuts at: 0 594 857 1608 Size: 594 263 751 Fragments arranged by size: 751 594 263 Bsu36I CC'TnA_GG Cuts at: 0 918 1608 Size: 918 690 BtgI C'CryG_G Cuts at: 0 62 295 806 1608 Size: 62 233 511 802 Fragments arranged by size: 802 511 233 62 BtsI GCAGTG_nn' Cuts at: 0 433 1608 Size: 433 1175 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 641 676 1608 Size: 641 35 932 Fragments arranged by size: 932 641 35 DraIII CAC_nnn'GTG Cuts at: 0 827 1375 1608 Size: 827 548 233 Fragments arranged by size: 827 548 233 EaeI y'GGCC_r Cuts at: 0 164 446 507 645 781 1608 Size: 164 282 61 138 136 827 Fragments arranged by size: 827 282 164 138 136 61 EarI CTCTTCn'nnn_ Cuts at: 0 173 1608 Size: 173 1435 EcoICRI GAG'CTC Cuts at: 0 529 1073 1608 Size: 529 544 535 Fragments arranged by size: 544 535 529 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 254 481 1189 1608 Size: 254 227 708 419 Fragments arranged by size: 708 419 254 227 EcoNI CCTnn'n_nnAGG Cuts at: 0 463 1222 1279 1608 Size: 463 759 57 329 Fragments arranged by size: 759 463 329 57 EcoO109I rG'GnC_Cy Cuts at: 0 288 441 1103 1104 1112 1215 1247 1469 Size: 288 153 662 1 8 103 32 222 Cuts at: 1469 1490 1608 Size: 21 118 Fragments arranged by size: 662 288 222 153 118 103 32 21 8 1 HaeII r_GCGC'y Cuts at: 0 612 843 1608 Size: 612 231 765 Fragments arranged by size: 765 612 231 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1159 1191 1608 Size: 1159 32 417 Fragments arranged by size: 1159 417 32 HincII GTy'rAC Cuts at: 0 1029 1608 Size: 1029 579 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1495 1608 Size: 1495 113 MscI TGG'CCA Cuts at: 0 166 448 647 1608 Size: 166 282 199 961 Fragments arranged by size: 961 282 199 166 MslI CAynn'nnrTG Cuts at: 0 1426 1608 Size: 1426 182 MspA1I CmG'CkG Cuts at: 0 619 765 1414 1608 Size: 619 146 649 194 Fragments arranged by size: 649 619 194 146 NaeI GCC'GGC Cuts at: 0 511 1608 Size: 511 1097 NcoI C'CATG_G Cuts at: 0 295 806 1608 Size: 295 511 802 Fragments arranged by size: 802 511 295 NdeI CA'TA_TG Cuts at: 0 342 1608 Size: 342 1266 NgoMIV G'CCGG_C Cuts at: 0 509 1608 Size: 509 1099 NspI r_CATG'y Cuts at: 0 118 564 1608 Size: 118 446 1044 Fragments arranged by size: 1044 446 118 PasI CC'CwG_GG Cuts at: 0 1004 1017 1061 1608 Size: 1004 13 44 547 Fragments arranged by size: 1004 547 44 13 PflMI CCAn_nnn'nTGG Cuts at: 0 765 894 1017 1608 Size: 765 129 123 591 Fragments arranged by size: 765 591 129 123 PfoI T'CCnGG_A Cuts at: 0 1544 1608 Size: 1544 64 PpuMI rG'GwC_Cy Cuts at: 0 288 441 1469 1490 1608 Size: 288 153 1028 21 118 Fragments arranged by size: 1028 288 153 118 21 PspOMI G'GGCC_C Cuts at: 0 1103 1112 1246 1608 Size: 1103 9 134 362 Fragments arranged by size: 1103 362 134 9 PstI C_TGCA'G Cuts at: 0 374 440 521 587 1226 1608 Size: 374 66 81 66 639 382 Fragments arranged by size: 639 382 374 81 66 66 PvuII CAG'CTG Cuts at: 0 619 765 1414 1608 Size: 619 146 649 194 Fragments arranged by size: 649 619 194 146 SacI G_AGCT'C Cuts at: 0 531 1075 1608 Size: 531 544 533 Fragments arranged by size: 544 533 531 SexAI A'CCwGG_T Cuts at: 0 1371 1608 Size: 1371 237 SfcI C'TryA_G Cuts at: 0 370 436 517 583 1222 1231 1608 Size: 370 66 81 66 639 9 377 Fragments arranged by size: 639 377 370 81 66 66 9 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1111 1608 Size: 1111 497 SphI G_CATG'C Cuts at: 0 118 1608 Size: 118 1490 StuI AGG'CCT Cuts at: 0 161 196 1608 Size: 161 35 1412 Fragments arranged by size: 1412 161 35 StyI C'CwwG_G Cuts at: 0 295 806 1107 1275 1337 1608 Size: 295 511 301 168 62 271 Fragments arranged by size: 511 301 295 271 168 62 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1550 1608 Size: 1550 58 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 859 891 1608 Size: 859 32 717 Fragments arranged by size: 859 717 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 456 1086 1379 1608 Size: 456 630 293 229 Fragments arranged by size: 630 456 293 229 Enzymes that do cut: AcuI AgeI AlfI AlwNI ApaI ApoI BaeI BanI BanII BbsI BclI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BsrBI BsrDI BsrFI BssSI BstAPI Bsu36I BtgI BtsI CspCI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SexAI SfcI SfiI SphI StuI StyI TaqII TstI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AhdI AleI AloI ApaLI AscI AseI AsiSI AvaI AvrII BamHI BbeI BbvCI BcgI BciVI BfrBI BglII BmtI Bpu10I BpuEI BsaBI BsaHI BsiEI BsiWI BsmI BspEI BspMI BsrGI BssHII BstBI BstEII BstXI BstYI BstZ17I BtgZI ClaI DraI DrdI EagI EciI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI NarI NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI SwaI TatI Tth111I XbaI XhoI XmaI XmnI ZraI