Restriction map for
pFN21AE2072
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspHI AvaI AviII AvrII
BanI BanII BbeI BbrPI BbvCI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BseAI
BseRI BsiHKAI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CelII ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV FseI FspI
HaeII Hin1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflMI PmaCI
PmeI PmlI PshAI Psp1406I PstI
RsrII SacI SacII SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 1248 GTCGAC GT^[AC][GT]AC
AgeI 716 ACCGGT A^CCGGT
AocI 196 CCTGAGG CC^T[ACGT]AGG
AseI 939 ATTAAT AT^TAAT
AsnI 939 ATTAAT AT^TAAT
Asp700I 452 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1282 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1013 TGGCCA TGG^CCA
BamHI 324 GGATCC G^GATCC
BcgI 221 CGACCACACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2202 GGATAC GTATCC(6/5)
BclI 1344 TGATCA T^GATCA
BcnI 765 CCGGG CC^[GC]GG
BmyI 376 GGGCAC G[AGT]GC[ACT]^C
BsaWI 716 ACCGGT [AT]^CCGG[AT]
BsiEI 1650 CGATCG CG[GA][CT]^CG
Bsp1286I 376 GGGCAC G[AGT]GC[ACT]^C
BsrBI 1777 GAGCGG CCGCTC(-3/-3)
BsrFI 716 ACCGGT [GA]^CCGG[CT]
Bsu36I 196 CCTGAGG CC^T[ACGT]AGG
CfoI 387 GCGC GCG^C
Cfr10I 716 ACCGGT [GA]^CCGG[CT]
DsaI 2198 CCGTGG C^C[GA][CT]GG
EaeI 1013 TGGCCA [CT]^GGCC[GA]
Eco81I 196 CCTGAGG CC^T[ACGT]AGG
FauI 1779 GCGGG CCCGC(4/6)
FbaI 1344 TGATCA T^GATCA
HhaI 387 GCGC GCG^C
HinP1I 387 GCGC G^CGC
MluNI 1013 TGGCCA TGG^CCA
MscI 1013 TGGCCA TGG^CCA
NciI 765 CCGGG CC^[GC]GG
NdeI 1564 CATATG CA^TATG
NspI 1093 ACATGC [GA]CATG^[CT]
PflFI 1282 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 716 ACCGGT A^CCGGT
PshBI 939 ATTAAT AT^TAAT
PvuI 1650 CGATCG CGAT^CG
SalI 1248 GTCGAC G^TCGAC
SapI 338 GCTCTTC GCTCTTC(1/4)
SexAI 806 ACCAGGT A^CC[AT]GGT
SmlI 69 CTTGAG C^T[CT][GA]AG
Tth111I 1282 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1703 CCACCAAGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 452 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AcsI 1383 AAATTC [GA]^AATT[CT]
1753 GAATTT
ApoI 1383 AAATTC [GA]^AATT[CT]
1753 GAATTT
BsaI 700 GAGACC GGTCTC(1/5)
1124 GAGACC
BsgI 1225 CTGCAC GTGCAG(16/14)
1685 GTGCAG
BsmI 755 GAATGC GAATGC(1/-1)
1655 GCATTC
BspHI 911 TCATGA T^CATGA
1484 TCATGA
DrdI 711 GACCTACCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1858 GACTACATTGTC
EcoNI 301 CCTTCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1011 CCTGGCCAAGG
EcoT14I 1016 CCAAGG C^C[AT][AT]GG
1712 CCTTGG
EcoT22I 943 ATGCAT ATGCA^T
1095 ATGCAT
HapII 717 CCGG C^CGG
765 CCGG
HpaII 717 CCGG C^CGG
765 CCGG
MaeII 1205 ACGT A^CGT
1340 ACGT
MslI 478 CACGGCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1479 CATCTTCATG
MspI 717 CCGG C^CGG
765 CCGG
NsiI 943 ATGCAT ATGCA^T
1095 ATGCAT
PvuII 593 CAGCTG CAG^CTG
1325 CAGCTG
RcaI 911 TCATGA T^CATGA
1484 TCATGA
SfcI 81 CTACAG C^T[GA][CT]AG
405 CTACAG
StyI 1016 CCAAGG C^C[AT][AT]GG
1712 CCTTGG
Tsp45I 432 GTCAC ^GT[GC]AC
1475 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BbsI BbvI BfaI BpmI
BpuAI BsaJI BsiYI BslI BsmAI
BsmFI BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HgaI
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII