(Circular) (Six-base) MAPSORT of: pFN21AE2072.seq Check: 3749 from: 1 to: 6913 pFN21AE2072 6913 bp With 182 enzymes: SgfI * August 16, 2010 14:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4723 278 Size: 53 83 186 936 3187 2468 Fragments arranged by size: 3187 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3254 4256 1937 Size: 1317 1002 4594 Fragments arranged by size: 4594 1317 1002 Acc65I G'GTAC_C Cuts at: 4234 4234 Size: 6913 AclI AA'CG_TT Cuts at: 1563 5040 5413 1563 Size: 3477 373 3063 Fragments arranged by size: 3477 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3611 4092 4976 6360 6628 1632 Size: 1979 481 884 1384 268 1917 Fragments arranged by size: 1979 1917 1384 884 481 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6716 AflIII A'CryG_T Cuts at: 1203 5812 6789 1203 Size: 4609 977 1327 Fragments arranged by size: 4609 1327 977 AgeI A'CCGG_T Cuts at: 2721 5744 2721 Size: 3023 3890 AhdI GACnn_n'nnGTC Cuts at: 5642 5642 Size: 6913 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6913 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6913 AlwNI CAG_nnn'CTG Cuts at: 3833 3954 4014 4024 4656 6228 3833 Size: 121 60 10 632 1572 4518 Fragments arranged by size: 4518 1572 632 121 60 10 ApaLI G'TGCA_C Cuts at: 4970 6126 4970 Size: 1156 5757 ApoI r'AATT_y Cuts at: 1493 3388 3758 4222 4448 4600 6709 1493 Size: 1895 370 464 226 152 2109 1697 Fragments arranged by size: 2109 1895 1697 464 370 226 152 AseI AT'TA_AT Cuts at: 160 1032 2945 5467 160 Size: 872 1913 2522 1606 Fragments arranged by size: 2522 1913 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6913 AvaI C'yCGr_G Cuts at: 1870 1943 4238 1870 Size: 73 2295 4545 Fragments arranged by size: 4545 2295 73 BamHI G'GATC_C Cuts at: 2329 4243 4610 2329 Size: 1914 367 4632 Fragments arranged by size: 4632 1914 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4234 5689 618 Size: 325 217 627 2447 1455 1842 Fragments arranged by size: 2447 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4232 1391 Size: 2841 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3039 3198 3286 3599 4197 928 Size: 971 1140 159 88 313 598 3644 Fragments arranged by size: 3644 1140 971 598 313 159 88 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2215 2249 5104 5138 1903 Size: 34 278 34 2855 34 3678 Fragments arranged by size: 3678 2855 278 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4201 4805 6015 4201 Size: 604 1210 5099 Fragments arranged by size: 5099 1210 604 BclI T'GATC_A Cuts at: 1548 3349 1548 Size: 1801 5112 BfrBI ATG'CAT Cuts at: 2950 3102 2950 Size: 152 6761 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5524 136 Size: 107 122 71 631 605 3852 1525 Fragments arranged by size: 3852 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6908 6908 Size: 6913 BlpI GC'TnA_GC Cuts at: 4330 4330 Size: 6913 Bme1580I G_kGCm'C Cuts at: 1790 2385 4974 6130 1790 Size: 595 2589 1156 2573 Fragments arranged by size: 2589 2573 1156 595 BmgBI CAC'GTC Cuts at: 1318 3211 1318 Size: 1893 5020 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3244 4644 5602 Size: 416 53 83 399 24 1827 1400 958 Cuts at: 5602 442 Size: 1753 Fragments arranged by size: 1827 1753 1400 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6913 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2143 2605 2693 3378 5573 1239 Size: 904 462 88 685 2195 2579 Fragments arranged by size: 2579 2195 904 685 462 88 Bpu10I CC'TnA_GC Cuts at: 2143 3698 3918 2143 Size: 1555 220 5138 Fragments arranged by size: 5138 1555 220 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2095 5038 5903 6201 2095 Size: 2943 865 298 2807 Fragments arranged by size: 2943 2807 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2699 3123 5576 882 Size: 617 1200 424 2453 2219 Fragments arranged by size: 2453 2219 1200 617 424 BsaAI yAC'GTr Cuts at: 493 1204 4704 493 Size: 711 3500 2702 Fragments arranged by size: 3500 2702 711 BsaBI GATnn'nnATC Cuts at: 6907 6907 Size: 6913 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4720 5102 5737 275 Size: 53 83 186 936 3187 382 635 1451 Fragments arranged by size: 3187 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2721 5346 5744 6018 6165 6576 2721 Size: 2625 398 274 147 411 3058 Fragments arranged by size: 3058 2625 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2122 2152 1178 Size: 30 165 30 719 30 5939 Fragments arranged by size: 5939 719 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6913 BseYI C'CCAG_C Cuts at: 2351 2596 6116 6612 2351 Size: 245 3520 496 2652 Fragments arranged by size: 3520 2652 496 245 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3215 3711 1163 Size: 573 1479 496 4365 Fragments arranged by size: 4365 1479 573 496 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3658 4363 5124 6152 1540 Size: 275 186 1657 705 761 1028 2301 Fragments arranged by size: 2301 1657 1028 761 705 275 186 BsiHKAI G_wGCw'C Cuts at: 4974 5059 6130 4974 Size: 85 1071 5757 Fragments arranged by size: 5757 1071 85 BsmI GAATG_Cn' Cuts at: 2766 3660 4424 4517 2766 Size: 894 764 93 5162 Fragments arranged by size: 5162 894 764 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2385 4232 4974 5059 6130 1391 Size: 399 595 1847 742 85 1071 2174 Fragments arranged by size: 2174 1847 1071 742 595 399 85 BspEI T'CCGG_A Cuts at: 6576 6576 Size: 6913 BspHI T'CATG_A Cuts at: 2916 3489 4802 6532 2916 Size: 573 1313 1730 3297 Fragments arranged by size: 3297 1730 1313 573 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4268 844 Size: 3424 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3784 4800 1394 Size: 429 1961 1016 3507 Fragments arranged by size: 3507 1961 1016 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2256 2667 3740 4179 5408 5582 65 Size: 1183 1008 411 1073 439 1229 174 1396 Fragments arranged by size: 1396 1229 1183 1073 1008 439 411 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2721 5557 5744 1700 Size: 110 911 2836 187 2869 Fragments arranged by size: 2869 2836 911 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6913 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6913 BssSI C'ACGA_G Cuts at: 4973 5985 4973 Size: 1012 5901 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6913 BstBI TT'CG_AA Cuts at: 5717 5717 Size: 6913 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6913 BstXI CCAn_nnnn'nTGG Cuts at: 2930 3028 3718 2930 Size: 98 690 6125 Fragments arranged by size: 6125 690 98 BstYI r'GATC_y Cuts at: 1972 2329 2865 3309 4243 4610 4995 5012 6464 Size: 357 536 444 934 367 385 17 1452 Cuts at: 6464 6550 6562 6908 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 934 536 444 385 367 357 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2202 2202 Size: 6913 BtgI C'CryG_G Cuts at: 513 1065 4203 513 Size: 552 3138 3223 Fragments arranged by size: 3223 3138 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2462 2502 487 Size: 683 56 1236 40 4898 Fragments arranged by size: 4898 1236 683 56 40 BtsI GCAGTG_nn' Cuts at: 2231 4429 5223 5243 2231 Size: 2198 794 20 3901 Fragments arranged by size: 3901 2198 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6913 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6878 DraI TTT'AAA Cuts at: 4217 4217 Size: 6913 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2722 3869 5920 809 Size: 507 1406 1147 2051 1802 Fragments arranged by size: 2051 1802 1406 1147 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3018 4360 5249 Size: 54 1425 50 275 18 1188 1342 889 Cuts at: 5249 6878 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1342 1188 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4360 1537 Size: 275 2548 4090 Fragments arranged by size: 4090 2548 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2350 2467 2472 4843 1353 Size: 997 117 5 2371 3423 Fragments arranged by size: 3423 2371 997 117 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5500 5874 6020 387 Size: 1064 138 3911 374 146 1280 Fragments arranged by size: 3911 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2143 2605 2693 3378 3611 4092 4976 Size: 393 511 462 88 685 233 481 884 Cuts at: 4976 5573 6360 6628 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 884 787 685 597 511 481 462 393 268 233 88 EcoNI CCTnn'n_nnAGG Cuts at: 2310 3020 2310 Size: 710 6203 EcoO109I rG'GnC_Cy Cuts at: 2518 2592 3667 4076 2518 Size: 74 1075 409 5355 Fragments arranged by size: 5355 1075 409 74 EcoRI G'AATT_C Cuts at: 4222 4600 4222 Size: 378 6535 FspI TGC'GCA Cuts at: 5419 5419 Size: 6913 HaeII r_GCGC'y Cuts at: 1678 6060 1678 Size: 4382 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2488 2520 2855 2857 2887 Size: 32 135 32 916 32 335 2 30 Cuts at: 2887 2889 5554 5586 5628 5660 1373 Size: 2 2665 32 42 32 2626 Fragments arranged by size: 2665 2626 916 335 135 42 32 32 32 32 32 30 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 2629 2995 3255 4257 4503 669 Size: 988 281 691 366 260 1002 246 3079 Fragments arranged by size: 3079 1002 988 691 366 281 260 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6913 HpaI GTT'AAC Cuts at: 4503 4503 Size: 6913 KpnI G_GTAC'C Cuts at: 4238 4238 Size: 6913 MfeI C'AATT_G Cuts at: 4512 4512 Size: 6913 MluI A'CGCG_T Cuts at: 6789 6789 Size: 6913 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2297 3408 6027 6211 1305 Size: 992 1111 2619 184 2007 Fragments arranged by size: 2619 2007 1111 992 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3020 10 Size: 54 1425 343 1188 3903 Fragments arranged by size: 3903 1425 1188 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2487 3488 4871 5230 5389 518 Size: 648 1321 1001 1383 359 159 2042 Fragments arranged by size: 2042 1383 1321 1001 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2351 2600 3332 4329 5006 6154 6399 1606 Size: 745 249 732 997 677 1148 245 2120 Fragments arranged by size: 2120 1148 997 745 732 677 249 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6361 NdeI CA'TA_TG Cuts at: 387 3570 387 Size: 3183 3730 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6913 NotI GC'GGCC_GC Cuts at: 4360 4360 Size: 6913 NsiI A_TGCA'T Cuts at: 2952 3104 2952 Size: 152 6761 NspI r_CATG'y Cuts at: 1132 1229 3102 4271 5816 1132 Size: 97 1873 1169 1545 2229 Fragments arranged by size: 2229 1873 1545 1169 97 PasI CC'CwG_GG Cuts at: 1348 4159 1348 Size: 2811 4102 PciI A'CATG_T Cuts at: 5812 5812 Size: 6913 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6913 PfoI T'CCnGG_A Cuts at: 3120 3355 3120 Size: 235 6678 PmeI GTTT'AAAC Cuts at: 4217 4217 Size: 6913 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4038 4070 4982 5014 6495 6527 4038 Size: 32 912 32 1481 32 4424 Fragments arranged by size: 4424 1481 912 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2518 2592 3667 4076 2518 Size: 74 1075 409 5355 Fragments arranged by size: 5355 1075 409 74 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6913 PsiI TTA'TAA Cuts at: 3170 4483 3170 Size: 1313 5600 PstI C_TGCA'G Cuts at: 830 4265 830 Size: 3435 3478 PvuI CG_AT'CG Cuts at: 2001 3658 2001 Size: 1657 5256 PvuII CAG'CTG Cuts at: 2600 3332 2600 Size: 732 6181 SalI G'TCGA_C Cuts at: 1936 3253 4255 1936 Size: 1317 1002 4594 Fragments arranged by size: 4594 1317 1002 SapI GCTCTTCn'nnn_ Cuts at: 2350 2350 Size: 6913 SbfI CC_TGCA'GG Cuts at: 4265 4265 Size: 6913 ScaI AGT'ACT Cuts at: 5161 6873 5161 Size: 1712 5201 SexAI A'CCwGG_T Cuts at: 2811 2811 Size: 6913 SfcI C'TryA_G Cuts at: 826 1045 2086 2410 4261 5396 6077 6268 826 Size: 219 1041 324 1851 1135 681 191 1471 Fragments arranged by size: 1851 1471 1135 1041 681 324 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6913 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6913 SmaI CCC'GGG Cuts at: 1872 4240 1872 Size: 2368 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2074 5017 5918 6180 820 Size: 197 926 131 2943 901 262 1553 Fragments arranged by size: 2943 1553 926 901 262 197 131 SnaBI TAC'GTA Cuts at: 493 4704 493 Size: 4211 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6913 SphI G_CATG'C Cuts at: 1132 4271 1132 Size: 3139 3774 SspI AAT'ATT Cuts at: 5 52 4837 5 Size: 47 4785 2081 Fragments arranged by size: 4785 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6913 StyI C'CwwG_G Cuts at: 513 1065 3021 3717 4352 513 Size: 552 1956 696 635 3074 Fragments arranged by size: 3074 1956 696 635 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4940 4957 5110 5295 5740 325 Size: 1255 3360 17 153 185 445 1498 Fragments arranged by size: 3360 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5159 6871 96 Size: 274 80 33 51 1443 3182 1712 138 Fragments arranged by size: 3182 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6873 6905 6873 Size: 32 6881 Tth111I GACn'n_nGTC Cuts at: 1316 3290 1316 Size: 1974 4939 XbaI T'CTAG_A Cuts at: 4249 4249 Size: 6913 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3715 3715 Size: 6913 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6913 XmaI C'CCGG_G Cuts at: 1870 4238 1870 Size: 2368 4545 XmnI GAAnn'nnTTC Cuts at: 2461 5042 2461 Size: 2581 4332 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4721 276 Size: 53 83 186 936 3187 2468 Fragments arranged by size: 3187 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI