(Linear) (Six-base) MAPSORT of: KIEE2072.seq Check: 8939 from: 1 to: 2208 KIEE2072.orf With 182 enzymes: SgfI * July 15, 2010 18:54 .. AccI GT'mk_AC Cuts at: 0 1249 2208 Size: 1249 959 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1606 2087 2208 Size: 1606 481 121 Fragments arranged by size: 1606 481 121 AgeI A'CCGG_T Cuts at: 0 716 2208 Size: 716 1492 AlwNI CAG_nnn'CTG Cuts at: 0 1828 1949 2009 2019 2208 Size: 1828 121 60 10 189 Fragments arranged by size: 1828 189 121 60 10 ApoI r'AATT_y Cuts at: 0 1383 1753 2208 Size: 1383 370 455 Fragments arranged by size: 1383 455 370 AseI AT'TA_AT Cuts at: 0 940 2208 Size: 940 1268 BamHI G'GATC_C Cuts at: 0 324 2208 Size: 324 1884 BbsI GAAGACnn'nnnn_ Cuts at: 0 1034 1193 1281 1594 2192 2208 Size: 1034 159 88 313 598 16 Fragments arranged by size: 1034 598 313 159 88 16 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 210 244 2208 Size: 210 34 1964 Fragments arranged by size: 1964 210 34 BciVI GTATCCnnnnn_n' Cuts at: 0 2196 2208 Size: 2196 12 BclI T'GATC_A Cuts at: 0 1344 2208 Size: 1344 864 BfrBI ATG'CAT Cuts at: 0 945 1097 2208 Size: 945 152 1111 Fragments arranged by size: 1111 945 152 Bme1580I G_kGCm'C Cuts at: 0 380 2208 Size: 380 1828 BmgBI CAC'GTC Cuts at: 0 1206 2208 Size: 1206 1002 BmrI ACTGGGnnnn_n' Cuts at: 0 1239 2208 Size: 1239 969 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 138 600 688 1373 2208 Size: 138 462 88 685 835 Fragments arranged by size: 835 685 462 138 88 Bpu10I CC'TnA_GC Cuts at: 0 138 1693 1913 2208 Size: 138 1555 220 295 Fragments arranged by size: 1555 295 220 138 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 90 2208 Size: 90 2118 BsaI GGTCTCn'nnnn_ Cuts at: 0 694 1118 2208 Size: 694 424 1090 Fragments arranged by size: 1090 694 424 BsaWI w'CCGG_w Cuts at: 0 716 2208 Size: 716 1492 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 117 147 2208 Size: 117 30 2061 Fragments arranged by size: 2061 117 30 BseYI C'CCAG_C Cuts at: 0 346 591 2208 Size: 346 245 1617 Fragments arranged by size: 1617 346 245 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1210 1706 2208 Size: 1210 496 502 Fragments arranged by size: 1210 502 496 BsiEI CG_ry'CG Cuts at: 0 1653 2208 Size: 1653 555 BsmI GAATG_Cn' Cuts at: 0 761 1655 2208 Size: 761 894 553 Fragments arranged by size: 894 761 553 Bsp1286I G_dGCh'C Cuts at: 0 380 2208 Size: 380 1828 BspHI T'CATG_A Cuts at: 0 911 1484 2208 Size: 911 573 724 Fragments arranged by size: 911 724 573 BsrBI CCG'CTC Cuts at: 0 1779 2208 Size: 1779 429 BsrDI GCAATG_nn' Cuts at: 0 251 662 1735 2174 2208 Size: 251 411 1073 439 34 Fragments arranged by size: 1073 439 411 251 34 BsrFI r'CCGG_y Cuts at: 0 716 2208 Size: 716 1492 BstXI CCAn_nnnn'nTGG Cuts at: 0 925 1023 1713 2208 Size: 925 98 690 495 Fragments arranged by size: 925 690 495 98 BstYI r'GATC_y Cuts at: 0 324 860 1304 2208 Size: 324 536 444 904 Fragments arranged by size: 904 536 444 324 Bsu36I CC'TnA_GG Cuts at: 0 197 2208 Size: 197 2011 BtgI C'CryG_G Cuts at: 0 2198 2208 Size: 2198 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 457 497 2208 Size: 457 40 1711 Fragments arranged by size: 1711 457 40 BtsI GCAGTG_nn' Cuts at: 0 226 2208 Size: 226 1982 DrdI GACnn_nn'nnGTC Cuts at: 0 717 1864 2208 Size: 717 1147 344 Fragments arranged by size: 1147 717 344 EaeI y'GGCC_r Cuts at: 0 1013 2208 Size: 1013 1195 EarI CTCTTCn'nnn_ Cuts at: 0 345 462 467 2208 Size: 345 117 5 1741 Fragments arranged by size: 1741 345 117 5 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 138 600 688 1373 1606 2087 2208 Size: 138 462 88 685 233 481 121 Fragments arranged by size: 685 481 462 233 138 121 88 EcoNI CCTnn'n_nnAGG Cuts at: 0 305 1015 2208 Size: 305 710 1193 Fragments arranged by size: 1193 710 305 EcoO109I rG'GnC_Cy Cuts at: 0 513 587 1662 2071 2208 Size: 513 74 1075 409 137 Fragments arranged by size: 1075 513 409 137 74 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 483 515 850 852 882 884 2208 Size: 483 32 335 2 30 2 1324 Fragments arranged by size: 1324 483 335 32 30 2 2 HincII GTy'rAC Cuts at: 0 624 990 1250 2208 Size: 624 366 260 958 Fragments arranged by size: 958 624 366 260 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 292 1403 2208 Size: 292 1111 805 Fragments arranged by size: 1111 805 292 MscI TGG'CCA Cuts at: 0 1015 2208 Size: 1015 1193 MslI CAynn'nnrTG Cuts at: 0 482 1483 2208 Size: 482 1001 725 Fragments arranged by size: 1001 725 482 MspA1I CmG'CkG Cuts at: 0 346 595 1327 2208 Size: 346 249 732 881 Fragments arranged by size: 881 732 346 249 NdeI CA'TA_TG Cuts at: 0 1565 2208 Size: 1565 643 NsiI A_TGCA'T Cuts at: 0 947 1099 2208 Size: 947 152 1109 Fragments arranged by size: 1109 947 152 NspI r_CATG'y Cuts at: 0 1097 2208 Size: 1097 1111 PasI CC'CwG_GG Cuts at: 0 2154 2208 Size: 2154 54 PfoI T'CCnGG_A Cuts at: 0 1115 1350 2208 Size: 1115 235 858 Fragments arranged by size: 1115 858 235 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2033 2065 2208 Size: 2033 32 143 Fragments arranged by size: 2033 143 32 PpuMI rG'GwC_Cy Cuts at: 0 513 587 1662 2071 2208 Size: 513 74 1075 409 137 Fragments arranged by size: 1075 513 409 137 74 PsiI TTA'TAA Cuts at: 0 1165 2208 Size: 1165 1043 PvuI CG_AT'CG Cuts at: 0 1653 2208 Size: 1653 555 PvuII CAG'CTG Cuts at: 0 595 1327 2208 Size: 595 732 881 Fragments arranged by size: 881 732 595 SalI G'TCGA_C Cuts at: 0 1248 2208 Size: 1248 960 SapI GCTCTTCn'nnn_ Cuts at: 0 345 2208 Size: 345 1863 SexAI A'CCwGG_T Cuts at: 0 806 2208 Size: 806 1402 SfcI C'TryA_G Cuts at: 0 81 405 2208 Size: 81 324 1803 Fragments arranged by size: 1803 324 81 SmlI C'TyrA_G Cuts at: 0 69 2208 Size: 69 2139 StyI C'CwwG_G Cuts at: 0 1016 1712 2208 Size: 1016 696 496 Fragments arranged by size: 1016 696 496 Tth111I GACn'n_nGTC Cuts at: 0 1285 2208 Size: 1285 923 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1710 2208 Size: 1710 498 XmnI GAAnn'nnTTC Cuts at: 0 456 2208 Size: 456 1752 Enzymes that do cut: AccI AcuI AgeI AlwNI ApoI AseI BamHI BbsI BcgI BciVI BclI BfrBI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseYI BsgI BsiEI BsmI Bsp1286I BspHI BsrBI BsrDI BsrFI BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EarI Eco57MI EcoNI EcoO109I Hin4I HincII MmeI MscI MslI MspA1I NdeI NsiI NspI PasI PfoI PpiI PpuMI PsiI PvuI PvuII SalI SapI SexAI SfcI SmlI StyI Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BanI BanII BbeI BbvCI BglI BglII BlpI BmtI BplI BsaAI BsaBI BsaHI BseRI BsiHKAI BsiWI BsmBI BspEI BspMI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PciI PflMI PmeI PmlI PshAI PspOMI PspXI PsrI PstI RsrII SacI SacII SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI TstI XbaI XhoI XmaI ZraI