Restriction map for
pFN21AE2108
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# NO CUT ENZYMES ( 89 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AocI Aor51HI AscI Asp718I AviII
AvrII BamHI BbrPI BbvCI BfrI
BglII BlnI BlpI Bpu1102I BsaI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI FseI FspI
HgaI HindIII HpaI KpnI KspI
MluI MroI NaeI NgoMIV NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SmlI SnaBI SphI Sse8387I
StuI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 1772 GACGTC GACGT^C
AhdI 1690 GACCCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 117 GTGCAC G^TGCAC
AlwNI 1032 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 957 GGGCCC GGGCC^C
ApaLI 117 GTGCAC G^TGCAC
AseI 2173 ATTAAT AT^TAAT
AsnI 2173 ATTAAT AT^TAAT
AspEI 1690 GACCCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 117 GTGCAC G[AT]GC[AT]^C
AvaI 56 CCCGGG C^[CT]CG[GA]G
BcgI 636 CGACGATGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2178 TGATCA T^GATCA
BglI 2308 GCCGCATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2490 CACGTA [CT]AC^GT[GA]
BsaWI 73 ACCGGA [AT]^CCGG[AT]
BseRI 606 CTCCTC GAGGAG(10/8)
BsiHKAI 117 GTGCAC G[AT]GC[AT]^C
BsoBI 56 CCCGGG C^[CT]CG[GA]G
BspHI 2082 TCATGA T^CATGA
BspMI 478 ACCTGC ACCTGC(4/8)
BsrBI 63 GAGCGG CCGCTC(-3/-3)
BstAPI 1546 GCAGCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 708 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2068 GACATCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 873 CCATGG C^C[GA][CT]GG
Eam1105I 1690 GACCCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 873 CCATGG C^C[AT][AT]GG
EcoT22I 990 ATGCAT ATGCA^T
FauI 567 CCCGC CCCGC(4/6)
FbaI 2178 TGATCA T^GATCA
MfeI 1490 CAATTG C^AATTG
MslI 1680 CATAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1490 CAATTG C^AATTG
NcoI 873 CCATGG C^CATGG
NdeI 325 CATATG CA^TATG
NheI 2031 GCTAGC G^CTAGC
NsiI 990 ATGCAT ATGCA^T
PleI 670 GAGTC GAGTC(4/5)
PshBI 2173 ATTAAT AT^TAAT
RcaI 2082 TCATGA T^CATGA
ScaI 1908 AGTACT AGT^ACT
SmaI 56 CCCGGG CCC^GGG
SpeI 1389 ACTAGT A^CTAGT
SspI 1953 AATATT AAT^ATT
StyI 873 CCATGG C^C[AT][AT]GG
XmaCI 56 CCCGGG C^CCGGG
XmaI 56 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccII 12 CGCG CG^CG
39 CGCG
Asp700I 1443 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1758 GAATGGATTC
AspI 904 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1528 GACAATGTC
BanII 957 GGGCCC G[GA]GC[CT]^C
1048 GAGCCC
BciVI 380 GTATCC GTATCC(6/5)
896 GTATCC
BsaBI 274 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2521 GATGATGATC
Bsp1407I 1568 TGTACA T^GTACA
2091 TGTACA
BsrGI 1568 TGTACA T^GTACA
2091 TGTACA
BstUI 12 CGCG CG^CG
39 CGCG
BstXI 385 CCAGTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1679 CCATAGCTGTGG
BstYI 279 AGATCC [GA]^GATC[CT]
1281 GGATCT
EarI 1343 GAAGAG CTCTTC(1/4)
2440 GAAGAG
EcoRV 997 GATATC GAT^ATC
1792 GATATC
Ksp632I 1343 GAAGAG CTCTTC(1/4)
2440 GAAGAG
MamI 274 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2521 GATGATGATC
MflI 279 AGATCC [GA]^GATC[CT]
1281 GGATCT
MvnI 12 CGCG CG^CG
39 CGCG
PflFI 904 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1528 GACAATGTC
PpuMI 779 GGGACCC [GA]G^G[AT]CC[CT]
2444 AGGACCT
SspBI 1568 TGTACA T^GTACA
2091 TGTACA
Tth111I 904 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1528 GACAATGTC
XcmI 114 CCAGTGCACAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
732 CCAGTGCGGCTCTGG
XhoII 279 AGATCC [GA]^GATC[CT]
1281 GGATCT
XmnI 1443 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1758 GAATGGATTC
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# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI AvaII
BalI BanI BbeI BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaJI BsgI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspLU11I
BsrDI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MspA1I MspI
MvaI MwoI NarI NciI NdeII
NlaIII NlaIV NspI PflMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I