(Circular) (Six-base) MAPSORT of: pFN21AE2108.seq Check: 2597 from: 1 to: 7240 pFN21AE2108 7240 bp With 182 enzymes: SgfI * August 16, 2010 14:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2492 2492 Size: 7240 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3781 5050 278 Size: 53 83 186 936 2245 1269 2468 Fragments arranged by size: 2468 2245 1269 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3514 3975 4471 4583 1937 Size: 1577 461 496 112 4594 Fragments arranged by size: 4594 1577 496 461 112 Acc65I G'GTAC_C Cuts at: 4561 4561 Size: 7240 AclI AA'CG_TT Cuts at: 1563 5367 5740 1563 Size: 3804 373 3063 Fragments arranged by size: 3804 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3469 4464 5303 6687 6955 1632 Size: 1837 995 839 1384 268 1917 Fragments arranged by size: 1917 1837 1384 995 839 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7043 AflIII A'CryG_T Cuts at: 1203 2216 2462 3517 3870 4494 6139 7116 1203 Size: 1013 246 1055 353 624 1645 977 1327 Fragments arranged by size: 1645 1327 1055 1013 977 624 353 246 AgeI A'CCGG_T Cuts at: 6071 6071 Size: 7240 AhdI GACnn_n'nnGTC Cuts at: 3700 5969 3700 Size: 2269 4971 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7240 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7240 AlwNI CAG_nnn'CTG Cuts at: 3042 4983 6555 3042 Size: 1941 1572 3727 Fragments arranged by size: 3727 1941 1572 ApaI G_GGCC'C Cuts at: 2966 2966 Size: 7240 ApaLI G'TGCA_C Cuts at: 2122 5297 6453 2122 Size: 3175 1156 2909 Fragments arranged by size: 3175 2909 1156 ApoI r'AATT_y Cuts at: 1493 2972 3228 4019 4549 4775 4927 7036 1493 Size: 1479 256 791 530 226 152 2109 1697 Fragments arranged by size: 2109 1697 1479 791 530 256 226 152 AseI AT'TA_AT Cuts at: 160 1032 4179 5794 160 Size: 872 3147 1615 1606 Fragments arranged by size: 3147 1615 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7240 AvaI C'yCGr_G Cuts at: 1870 1943 2061 4565 1870 Size: 73 118 2504 4545 Fragments arranged by size: 4545 2504 118 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4260 4293 4481 4514 4260 Size: 33 188 33 6986 Fragments arranged by size: 6986 188 33 33 BamHI G'GATC_C Cuts at: 4570 4937 4570 Size: 367 6873 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2056 2072 2366 2585 4010 Size: 325 217 627 269 16 294 219 1425 Cuts at: 4010 4561 6016 618 Size: 551 1455 1842 Fragments arranged by size: 1842 1455 1425 627 551 325 294 269 219 217 16 BanII G_rGCy'C Cuts at: 1391 2966 3057 4559 1391 Size: 1575 91 1502 4072 Fragments arranged by size: 4072 1575 1502 91 BbeI G_GCGC'C Cuts at: 2060 2076 2370 2589 2060 Size: 16 294 219 6711 Fragments arranged by size: 6711 294 219 16 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2178 2219 4134 928 Size: 971 279 41 1915 4034 Fragments arranged by size: 4034 1915 971 279 41 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2630 2664 5431 5465 1903 Size: 34 693 34 2767 34 3678 Fragments arranged by size: 3678 2767 693 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2396 2912 5132 6342 2396 Size: 516 2220 1210 3294 Fragments arranged by size: 3294 2220 1210 516 BclI T'GATC_A Cuts at: 1548 4183 1548 Size: 2635 4605 BfrBI ATG'CAT Cuts at: 2997 2997 Size: 7240 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4319 5851 136 Size: 107 122 71 631 605 2647 1532 1525 Fragments arranged by size: 2647 1532 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7235 7235 Size: 7240 BlpI GC'TnA_GC Cuts at: 4657 4657 Size: 7240 Bme1580I G_kGCm'C Cuts at: 1790 2012 2126 2572 2966 5301 6457 1790 Size: 222 114 446 394 2335 1156 2573 Fragments arranged by size: 2573 2335 1156 446 394 222 114 BmgBI CAC'GTC Cuts at: 1318 2013 2217 1318 Size: 695 204 6341 Fragments arranged by size: 6341 695 204 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2384 3736 4209 Size: 416 53 83 399 24 967 1352 473 Cuts at: 4209 4971 5929 442 Size: 762 958 1753 Fragments arranged by size: 1753 1352 967 958 762 473 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4040 1056 Size: 2984 4256 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3697 4471 3697 Size: 774 6466 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2419 2473 2491 2702 3064 3947 5900 1239 Size: 1180 54 18 211 362 883 1953 2579 Fragments arranged by size: 2579 1953 1180 883 362 211 54 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5365 6230 6528 5365 Size: 865 298 6077 Fragments arranged by size: 6077 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5903 882 Size: 617 4404 2219 Fragments arranged by size: 4404 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4497 5031 493 Size: 711 3293 534 2702 Fragments arranged by size: 3293 2702 711 534 BsaBI GATnn'nnATC Cuts at: 2283 4530 7234 2283 Size: 2247 2704 2289 Fragments arranged by size: 2704 2289 2247 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2057 2073 2367 2586 Size: 53 83 186 936 524 16 294 219 Cuts at: 2586 3778 5047 5429 6064 275 Size: 1192 1269 382 635 1451 Fragments arranged by size: 1451 1269 1192 936 635 524 382 294 219 186 83 53 16 BsaWI w'CCGG_w Cuts at: 2078 5673 6071 6345 6492 6903 2078 Size: 3595 398 274 147 411 2415 Fragments arranged by size: 3595 2415 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7015 Fragments arranged by size: 7015 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2602 1186 Size: 1416 5824 BseYI C'CCAG_C Cuts at: 2052 2261 2631 3155 4501 6443 6939 2052 Size: 209 370 524 1346 1942 496 2353 Fragments arranged by size: 2353 1942 1346 524 496 370 209 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2623 3001 3171 1163 Size: 573 887 378 170 5232 Fragments arranged by size: 5232 887 573 378 170 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4690 5451 6479 1540 Size: 275 186 2689 761 1028 2301 Fragments arranged by size: 2689 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2126 5301 5386 6457 2126 Size: 3175 85 1071 2909 Fragments arranged by size: 3175 2909 1071 85 BsmI GAATG_Cn' Cuts at: 2999 3086 3206 4751 4844 2999 Size: 87 120 1545 93 5395 Fragments arranged by size: 5395 1545 120 93 87 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2126 2572 2966 3057 4559 5301 Size: 399 222 114 446 394 91 1502 742 Cuts at: 5301 5386 6457 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1502 1071 742 446 399 394 222 114 91 85 BspEI T'CCGG_A Cuts at: 6903 6903 Size: 7240 BspHI T'CATG_A Cuts at: 4087 5129 6859 4087 Size: 1042 1730 4468 Fragments arranged by size: 4468 1730 1042 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2492 4595 844 Size: 1648 2103 3489 Fragments arranged by size: 3489 2103 1648 BsrBI CCG'CTC Cuts at: 1394 1823 2070 5127 1394 Size: 429 247 3057 3507 Fragments arranged by size: 3507 3057 429 247 BsrDI GCAATG_nn' Cuts at: 65 1248 2530 3435 3555 5735 5909 65 Size: 1183 1282 905 120 2180 174 1396 Fragments arranged by size: 2180 1396 1282 1183 905 174 120 BsrFI r'CCGG_y Cuts at: 1700 1810 5884 6071 1700 Size: 110 4074 187 2869 Fragments arranged by size: 4074 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3573 4096 96 Size: 3477 523 3240 Fragments arranged by size: 3477 3240 523 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7240 BssSI C'ACGA_G Cuts at: 5300 6312 5300 Size: 1012 6228 BstAPI GCAn_nnn'nTGC Cuts at: 794 3557 794 Size: 2763 4477 BstBI TT'CG_AA Cuts at: 6044 6044 Size: 7240 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7240 BstXI CCAn_nnnn'nTGG Cuts at: 2397 3691 2397 Size: 1294 5946 BstYI r'GATC_y Cuts at: 1972 2284 3286 4570 4937 5322 5339 6791 6877 Size: 312 1002 1284 367 385 17 1452 86 Cuts at: 6877 6889 7235 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1284 1002 385 367 346 312 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2878 513 Size: 552 939 874 4875 Fragments arranged by size: 4875 939 874 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2559 2788 487 Size: 683 56 1333 229 4939 Fragments arranged by size: 4939 1333 683 229 56 BtsI GCAGTG_nn' Cuts at: 2194 2557 2693 2812 2897 4756 5550 5570 2194 Size: 363 136 119 85 1859 794 20 3864 Fragments arranged by size: 3864 1859 794 363 136 119 85 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7240 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7205 DraI TTT'AAA Cuts at: 4544 4544 Size: 7240 DraIII CAC_nnn'GTG Cuts at: 2718 2718 Size: 7240 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4079 6247 809 Size: 507 2763 2168 1802 Fragments arranged by size: 2763 2168 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2248 2494 2527 Size: 54 1425 50 275 18 418 246 33 Cuts at: 2527 2692 3895 4240 4687 5576 7205 8 Size: 165 1203 345 447 889 1629 43 Fragments arranged by size: 1629 1425 1203 889 447 418 345 275 246 165 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 4687 1537 Size: 275 2875 4090 Fragments arranged by size: 4090 2875 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3343 4440 5170 1353 Size: 1990 1097 730 3423 Fragments arranged by size: 3423 1990 1097 730 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4393 5827 6201 6347 387 Size: 1064 138 2804 1434 374 146 1280 Fragments arranged by size: 2804 1434 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2419 2473 2491 2702 3064 3469 3947 Size: 393 787 54 18 211 362 405 478 Cuts at: 3947 4464 5303 5900 6687 6955 1239 Size: 517 839 597 787 268 1524 Fragments arranged by size: 1524 839 787 787 597 517 478 405 393 362 268 211 54 18 EcoO109I rG'GnC_Cy Cuts at: 2785 2963 4450 2785 Size: 178 1487 5575 Fragments arranged by size: 5575 1487 178 EcoRI G'AATT_C Cuts at: 4549 4927 4549 Size: 378 6862 EcoRV GAT'ATC Cuts at: 3004 3799 3004 Size: 795 6445 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3385 3499 3385 Size: 114 7126 FspI TGC'GCA Cuts at: 5746 5746 Size: 7240 HaeII r_GCGC'y Cuts at: 1678 2030 2060 2076 2370 2589 6387 1678 Size: 352 30 16 294 219 3798 2531 Fragments arranged by size: 3798 2531 352 294 219 30 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2898 2930 3686 3718 4168 Size: 32 135 32 1326 32 756 32 450 Cuts at: 4168 4200 5881 5913 5955 5987 1373 Size: 32 1681 32 42 32 2626 Fragments arranged by size: 2626 1681 1326 756 450 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2182 2818 2935 4051 4584 4830 Size: 988 281 244 636 117 1116 533 246 Cuts at: 4830 669 Size: 3079 Fragments arranged by size: 3079 1116 988 636 533 281 246 244 117 HindIII A'AGCT_T Cuts at: 748 748 Size: 7240 HpaI GTT'AAC Cuts at: 4830 4830 Size: 7240 KasI G'GCGC_C Cuts at: 2056 2072 2366 2585 2056 Size: 16 294 219 6711 Fragments arranged by size: 6711 294 219 16 KpnI G_GTAC'C Cuts at: 4565 4565 Size: 7240 MfeI C'AATT_G Cuts at: 3495 4839 3495 Size: 1344 5896 MluI A'CGCG_T Cuts at: 7116 7116 Size: 7240 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2685 3415 4468 6354 6538 1305 Size: 1380 730 1053 1886 184 2007 Fragments arranged by size: 2007 1886 1380 1053 730 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2250 2529 3897 10 Size: 54 1425 343 418 279 1368 3353 Fragments arranged by size: 3353 1425 1368 418 343 279 54 MslI CAynn'nnrTG Cuts at: 518 1166 3689 5198 5557 5716 518 Size: 648 2523 1509 359 159 2042 Fragments arranged by size: 2523 2042 1509 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2361 2499 2631 2871 3155 3794 4656 5333 Size: 755 138 132 240 284 639 862 677 Cuts at: 5333 6481 6726 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 862 755 677 639 284 245 240 138 132 NarI GG'CG_CC Cuts at: 2057 2073 2367 2586 2057 Size: 16 294 219 6711 Fragments arranged by size: 6711 294 219 16 NcoI C'CATG_G Cuts at: 513 1065 2004 2878 513 Size: 552 939 874 4875 Fragments arranged by size: 4875 939 874 552 NdeI CA'TA_TG Cuts at: 387 2331 387 Size: 1944 5296 NheI G'CTAG_C Cuts at: 1052 4036 1052 Size: 2984 4256 NotI GC'GGCC_GC Cuts at: 4687 4687 Size: 7240 NsiI A_TGCA'T Cuts at: 2999 2999 Size: 7240 NspI r_CATG'y Cuts at: 1132 1229 2343 2466 3521 3874 4598 6143 1132 Size: 97 1114 123 1055 353 724 1545 2229 Fragments arranged by size: 2229 1545 1114 1055 724 353 123 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7240 PciI A'CATG_T Cuts at: 2462 3517 3870 6139 2462 Size: 1055 353 2269 3563 Fragments arranged by size: 3563 2269 1055 353 PflMI CCAn_nnn'nTGG Cuts at: 1108 2268 2582 2631 3588 3704 1108 Size: 1160 314 49 957 116 4644 Fragments arranged by size: 4644 1160 957 314 116 49 PfoI T'CCnGG_A Cuts at: 2396 2507 3211 2396 Size: 111 704 6425 Fragments arranged by size: 6425 704 111 PmeI GTTT'AAAC Cuts at: 4544 4544 Size: 7240 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5309 5341 6822 6854 5309 Size: 32 1481 32 5695 Fragments arranged by size: 5695 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2785 4450 2785 Size: 1665 5575 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7240 PsiI TTA'TAA Cuts at: 3833 4810 3833 Size: 977 6263 PspOMI G'GGCC_C Cuts at: 2962 2962 Size: 7240 PstI C_TGCA'G Cuts at: 830 2543 3038 3224 3553 4592 830 Size: 1713 495 186 329 1039 3478 Fragments arranged by size: 3478 1713 1039 495 329 186 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7240 PvuII CAG'CTG Cuts at: 2361 2631 2871 3155 2361 Size: 270 240 284 6446 Fragments arranged by size: 6446 284 270 240 SalI G'TCGA_C Cuts at: 1936 4582 1936 Size: 2646 4594 SanDI GG'GwC_CC Cuts at: 2785 2785 Size: 7240 SbfI CC_TGCA'GG Cuts at: 4592 4592 Size: 7240 ScaI AGT'ACT Cuts at: 3915 5488 7200 3915 Size: 1573 1712 3955 Fragments arranged by size: 3955 1712 1573 SfcI C'TryA_G Cuts at: 826 1045 2539 3034 3220 3549 4588 5723 6404 Size: 219 1494 495 186 329 1039 1135 681 Cuts at: 6404 6595 826 Size: 191 1471 Fragments arranged by size: 1494 1471 1135 1039 681 495 329 219 191 186 SfoI GGC'GCC Cuts at: 2058 2074 2368 2587 2058 Size: 16 294 219 6711 Fragments arranged by size: 6711 294 219 16 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7240 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7240 SmaI CCC'GGG Cuts at: 1872 2063 4567 1872 Size: 191 2504 4545 Fragments arranged by size: 4545 2504 191 SmlI C'TyrA_G Cuts at: 820 1017 1943 5344 6245 6507 820 Size: 197 926 3401 901 262 1553 Fragments arranged by size: 3401 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5031 493 Size: 4538 2702 SpeI A'CTAG_T Cuts at: 152 3394 152 Size: 3242 3998 SphI G_CATG'C Cuts at: 1132 4598 1132 Size: 3466 3774 SspI AAT'ATT Cuts at: 5 52 3960 5164 5 Size: 47 3908 1204 2081 Fragments arranged by size: 3908 2081 1204 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7240 StyI C'CwwG_G Cuts at: 513 1065 2004 2878 4679 513 Size: 552 939 874 1801 3074 Fragments arranged by size: 3074 1801 939 874 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3559 5267 5284 5437 5622 6067 325 Size: 1255 1979 1708 17 153 185 445 1498 Fragments arranged by size: 1979 1708 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3573 3913 4096 Size: 274 80 33 51 1443 1596 340 183 Cuts at: 4096 4428 5486 7198 96 Size: 332 1058 1712 138 Fragments arranged by size: 1712 1596 1443 1058 340 332 274 183 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2704 2736 3169 3201 3788 3820 3860 3892 7200 Size: 32 433 32 587 32 40 32 3308 Cuts at: 7200 7232 2704 Size: 32 2712 Fragments arranged by size: 3308 2712 587 433 40 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2912 3536 1316 Size: 1596 624 5020 Fragments arranged by size: 5020 1596 624 XbaI T'CTAG_A Cuts at: 4576 4576 Size: 7240 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2126 2744 2126 Size: 618 6622 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7240 XmaI C'CCGG_G Cuts at: 1870 2061 4565 1870 Size: 191 2504 4545 Fragments arranged by size: 4545 2504 191 XmnI GAAnn'nnTTC Cuts at: 3452 3767 5369 3452 Size: 315 1602 5323 Fragments arranged by size: 5323 1602 315 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3779 5048 276 Size: 53 83 186 936 2245 1269 2468 Fragments arranged by size: 2468 2245 1269 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI FseI FspAI NaeI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI