(Linear) (Six-base) MAPSORT of: KIEE2108.seq Check: 8483 from: 1 to: 2535 KIEE2108.orf With 182 enzymes: SgfI * July 15, 2010 18:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 487 2535 Size: 487 2048 AatII G_ACGT'C Cuts at: 0 1776 2535 Size: 1776 759 AccI GT'mk_AC Cuts at: 0 1509 1970 2466 2535 Size: 1509 461 496 69 Fragments arranged by size: 1509 496 461 69 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1464 2459 2535 Size: 1464 995 76 Fragments arranged by size: 1464 995 76 AflIII A'CryG_T Cuts at: 0 211 457 1512 1865 2489 2535 Size: 211 246 1055 353 624 46 Fragments arranged by size: 1055 624 353 246 211 46 AhdI GACnn_n'nnGTC Cuts at: 0 1695 2535 Size: 1695 840 AlwNI CAG_nnn'CTG Cuts at: 0 1037 2535 Size: 1037 1498 ApaI G_GGCC'C Cuts at: 0 961 2535 Size: 961 1574 ApaLI G'TGCA_C Cuts at: 0 117 2535 Size: 117 2418 ApoI r'AATT_y Cuts at: 0 967 1223 2014 2535 Size: 967 256 791 521 Fragments arranged by size: 967 791 521 256 AseI AT'TA_AT Cuts at: 0 2174 2535 Size: 2174 361 AvaI C'yCGr_G Cuts at: 0 56 2535 Size: 56 2479 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 2255 2288 2476 2509 2535 Size: 2255 33 188 33 26 Fragments arranged by size: 2255 188 33 33 26 BanI G'GyrC_C Cuts at: 0 51 67 361 580 2005 2535 Size: 51 16 294 219 1425 530 Fragments arranged by size: 1425 530 294 219 51 16 BanII G_rGCy'C Cuts at: 0 961 1052 2535 Size: 961 91 1483 Fragments arranged by size: 1483 961 91 BbeI G_GCGC'C Cuts at: 0 55 71 365 584 2535 Size: 55 16 294 219 1951 Fragments arranged by size: 1951 294 219 55 16 BbsI GAAGACnn'nnnn_ Cuts at: 0 173 214 2129 2535 Size: 173 41 1915 406 Fragments arranged by size: 1915 406 173 41 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 625 659 2535 Size: 625 34 1876 Fragments arranged by size: 1876 625 34 BciVI GTATCCnnnnn_n' Cuts at: 0 391 907 2535 Size: 391 516 1628 Fragments arranged by size: 1628 516 391 BclI T'GATC_A Cuts at: 0 2178 2535 Size: 2178 357 BfrBI ATG'CAT Cuts at: 0 992 2535 Size: 992 1543 BglI GCCn_nnn'nGGC Cuts at: 0 2314 2535 Size: 2314 221 Bme1580I G_kGCm'C Cuts at: 0 7 121 567 961 2535 Size: 7 114 446 394 1574 Fragments arranged by size: 1574 446 394 114 7 BmgBI CAC'GTC Cuts at: 0 8 212 2535 Size: 8 204 2323 Fragments arranged by size: 2323 204 8 BmrI ACTGGGnnnn_n' Cuts at: 0 379 1731 2204 2535 Size: 379 1352 473 331 Fragments arranged by size: 1352 473 379 331 BmtI G_CTAG'C Cuts at: 0 2035 2535 Size: 2035 500 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1692 2466 2535 Size: 1692 774 69 Fragments arranged by size: 1692 774 69 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 414 468 486 697 1059 1942 2535 Size: 414 54 18 211 362 883 593 Fragments arranged by size: 883 593 414 362 211 54 18 BsaAI yAC'GTr Cuts at: 0 2492 2535 Size: 2492 43 BsaBI GATnn'nnATC Cuts at: 0 278 2525 2535 Size: 278 2247 10 Fragments arranged by size: 2247 278 10 BsaHI Gr'CG_yC Cuts at: 0 52 68 362 581 1773 2535 Size: 52 16 294 219 1192 762 Fragments arranged by size: 1192 762 294 219 52 16 BsaWI w'CCGG_w Cuts at: 0 73 2535 Size: 73 2462 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 597 2535 Size: 597 1938 BseYI C'CCAG_C Cuts at: 0 47 256 626 1150 2496 2535 Size: 47 209 370 524 1346 39 Fragments arranged by size: 1346 524 370 209 47 39 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 618 996 1166 2535 Size: 618 378 170 1369 Fragments arranged by size: 1369 618 378 170 BsiHKAI G_wGCw'C Cuts at: 0 121 2535 Size: 121 2414 BsmI GAATG_Cn' Cuts at: 0 994 1081 1201 2535 Size: 994 87 120 1334 Fragments arranged by size: 1334 994 120 87 Bsp1286I G_dGCh'C Cuts at: 0 7 121 567 961 1052 2535 Size: 7 114 446 394 91 1483 Fragments arranged by size: 1483 446 394 114 91 7 BspHI T'CATG_A Cuts at: 0 2082 2535 Size: 2082 453 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 487 2535 Size: 487 2048 BsrBI CCG'CTC Cuts at: 0 65 2535 Size: 65 2470 BsrDI GCAATG_nn' Cuts at: 0 525 1430 1550 2535 Size: 525 905 120 985 Fragments arranged by size: 985 905 525 120 BsrGI T'GTAC_A Cuts at: 0 1568 2091 2535 Size: 1568 523 444 Fragments arranged by size: 1568 523 444 BstAPI GCAn_nnn'nTGC Cuts at: 0 1552 2535 Size: 1552 983 BstXI CCAn_nnnn'nTGG Cuts at: 0 392 1686 2535 Size: 392 1294 849 Fragments arranged by size: 1294 849 392 BstYI r'GATC_y Cuts at: 0 279 1281 2535 Size: 279 1002 1254 Fragments arranged by size: 1254 1002 279 BtgI C'CryG_G Cuts at: 0 873 2535 Size: 873 1662 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 554 783 2535 Size: 554 229 1752 Fragments arranged by size: 1752 554 229 BtsI GCAGTG_nn' Cuts at: 0 189 552 688 807 892 2535 Size: 189 363 136 119 85 1643 Fragments arranged by size: 1643 363 189 136 119 85 DraIII CAC_nnn'GTG Cuts at: 0 713 2535 Size: 713 1822 DrdI GACnn_nn'nnGTC Cuts at: 0 2074 2535 Size: 2074 461 EaeI y'GGCC_r Cuts at: 0 243 489 522 687 1890 2235 2535 Size: 243 246 33 165 1203 345 300 Fragments arranged by size: 1203 345 300 246 243 165 33 EarI CTCTTCn'nnn_ Cuts at: 0 1338 2435 2535 Size: 1338 1097 100 Fragments arranged by size: 1338 1097 100 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2388 2535 Size: 2388 147 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 414 468 486 697 1059 1464 1942 2459 Size: 414 54 18 211 362 405 478 517 Cuts at: 2459 2535 Size: 76 Fragments arranged by size: 517 478 414 405 362 211 76 54 18 EcoO109I rG'GnC_Cy Cuts at: 0 780 958 2445 2535 Size: 780 178 1487 90 Fragments arranged by size: 1487 780 178 90 EcoRV GAT'ATC Cuts at: 0 999 1794 2535 Size: 999 795 741 Fragments arranged by size: 999 795 741 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1380 1494 2535 Size: 1380 114 1041 Fragments arranged by size: 1380 1041 114 HaeII r_GCGC'y Cuts at: 0 25 55 71 365 584 2535 Size: 25 30 16 294 219 1951 Fragments arranged by size: 1951 294 219 30 25 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 893 925 1681 1713 2163 2195 2535 Size: 893 32 756 32 450 32 340 Fragments arranged by size: 893 756 450 340 32 32 32 HincII GTy'rAC Cuts at: 0 177 813 930 2046 2535 Size: 177 636 117 1116 489 Fragments arranged by size: 1116 636 489 177 117 KasI G'GCGC_C Cuts at: 0 51 67 361 580 2535 Size: 51 16 294 219 1955 Fragments arranged by size: 1955 294 219 51 16 MfeI C'AATT_G Cuts at: 0 1490 2535 Size: 1490 1045 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 680 1410 2463 2535 Size: 680 730 1053 72 Fragments arranged by size: 1053 730 680 72 MscI TGG'CCA Cuts at: 0 245 524 1892 2535 Size: 245 279 1368 643 Fragments arranged by size: 1368 643 279 245 MslI CAynn'nnrTG Cuts at: 0 1684 2535 Size: 1684 851 MspA1I CmG'CkG Cuts at: 0 356 494 626 866 1150 1789 2535 Size: 356 138 132 240 284 639 746 Fragments arranged by size: 746 639 356 284 240 138 132 NarI GG'CG_CC Cuts at: 0 52 68 362 581 2535 Size: 52 16 294 219 1954 Fragments arranged by size: 1954 294 219 52 16 NcoI C'CATG_G Cuts at: 0 873 2535 Size: 873 1662 NdeI CA'TA_TG Cuts at: 0 326 2535 Size: 326 2209 NheI G'CTAG_C Cuts at: 0 2031 2535 Size: 2031 504 NsiI A_TGCA'T Cuts at: 0 994 2535 Size: 994 1541 NspI r_CATG'y Cuts at: 0 338 461 1516 1869 2535 Size: 338 123 1055 353 666 Fragments arranged by size: 1055 666 353 338 123 PciI A'CATG_T Cuts at: 0 457 1512 1865 2535 Size: 457 1055 353 670 Fragments arranged by size: 1055 670 457 353 PflMI CCAn_nnn'nTGG Cuts at: 0 263 577 626 1583 1699 2535 Size: 263 314 49 957 116 836 Fragments arranged by size: 957 836 314 263 116 49 PfoI T'CCnGG_A Cuts at: 0 391 502 1206 2535 Size: 391 111 704 1329 Fragments arranged by size: 1329 704 391 111 PpuMI rG'GwC_Cy Cuts at: 0 780 2445 2535 Size: 780 1665 90 Fragments arranged by size: 1665 780 90 PsiI TTA'TAA Cuts at: 0 1828 2535 Size: 1828 707 PspOMI G'GGCC_C Cuts at: 0 957 2535 Size: 957 1578 PstI C_TGCA'G Cuts at: 0 538 1033 1219 1548 2535 Size: 538 495 186 329 987 Fragments arranged by size: 987 538 495 329 186 PvuII CAG'CTG Cuts at: 0 356 626 866 1150 2535 Size: 356 270 240 284 1385 Fragments arranged by size: 1385 356 284 270 240 SanDI GG'GwC_CC Cuts at: 0 780 2535 Size: 780 1755 ScaI AGT'ACT Cuts at: 0 1910 2535 Size: 1910 625 SfcI C'TryA_G Cuts at: 0 534 1029 1215 1544 2535 Size: 534 495 186 329 991 Fragments arranged by size: 991 534 495 329 186 SfoI GGC'GCC Cuts at: 0 53 69 363 582 2535 Size: 53 16 294 219 1953 Fragments arranged by size: 1953 294 219 53 16 SmaI CCC'GGG Cuts at: 0 58 2535 Size: 58 2477 SpeI A'CTAG_T Cuts at: 0 1389 2535 Size: 1389 1146 SspI AAT'ATT Cuts at: 0 1955 2535 Size: 1955 580 StyI C'CwwG_G Cuts at: 0 873 2535 Size: 873 1662 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1554 2535 Size: 1554 981 TatI w'GTAC_w Cuts at: 0 1568 1908 2091 2423 2535 Size: 1568 340 183 332 112 Fragments arranged by size: 1568 340 332 183 112 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 699 731 1164 1196 1783 1815 1855 1887 Size: 699 32 433 32 587 32 40 32 Cuts at: 1887 2535 Size: 648 Fragments arranged by size: 699 648 587 433 40 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 907 1531 2535 Size: 907 624 1004 Fragments arranged by size: 1004 907 624 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 121 739 2535 Size: 121 618 1796 Fragments arranged by size: 1796 618 121 XmaI C'CCGG_G Cuts at: 0 56 2535 Size: 56 2479 XmnI GAAnn'nnTTC Cuts at: 0 1447 1762 2535 Size: 1447 315 773 Fragments arranged by size: 1447 773 315 ZraI GAC'GTC Cuts at: 0 1774 2535 Size: 1774 761 Enzymes that do cut: AarI AatII AccI AcuI AflIII AhdI AlwNI ApaI ApaLI ApoI AseI AvaI BaeI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI Bme1580I BmgBI BmrI BmtI BplI BpmI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrGI BstAPI BstXI BstYI BtgI BtgZI BtsI DraIII DrdI EaeI EarI EciI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I HincII KasI MfeI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NsiI NspI PciI PflMI PfoI PpuMI PsiI PspOMI PstI PvuII SanDI ScaI SfcI SfoI SmaI SpeI SspI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflII AgeI AleI AlfI AloI AscI AsiSI AvrII BamHI BbvCI BglII BlpI Bpu10I BpuEI BsaI BsaXI BsiEI BsiWI BsmBI BspEI BsrFI BssHII BssSI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI EagI EcoICRI EcoNI EcoRI FseI FspI FspAI HindIII HpaI KpnI MluI NaeI NgoMIV NotI NruI PacI PasI PmeI PmlI PpiI PshAI PspXI PsrI PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SgfI SgrAI SmlI SnaBI SphI SrfI StuI SwaI XbaI XhoI