################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## AatII Acc65I AccII AciI AclI AcyI AflII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BanI BbeI BbrPI BcgI BciVI BclI BcnI BfaI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BseRI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI BspDI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI KspI MaeI MfeI MluI MunI MvnI MwoI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I StuI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccIII 387 TCCGGA T^CCGGA AflIII 372 ACGTGT A^C[GA][CT]GT Alw44I 1144 GTGCAC G^TGCAC AlwNI 1108 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 928 GGGCCC GGGCC^C ApaLI 1144 GTGCAC G^TGCAC AspHI 1144 GTGCAC G[AT]GC[AT]^C BalI 666 TGGCCA TGG^CCA BamHI 420 GGATCC G^GATCC BanII 928 GGGCCC G[GA]GC[CT]^C BbsI 1051 GTCTTC GAAGAC(2/6) BbvCI 701 CCTCAGC CCTCAGC(-5/-2) BglII 500 AGATCT A^GATCT BpuAI 1051 GTCTTC GAAGAC(2/6) BsaBI 979 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 387 TCCGGA [AT]^CCGG[AT] BseAI 387 TCCGGA T^CCGGA BsgI 960 CTGCAC GTGCAG(16/14) BsiHKAI 1144 GTGCAC G[AT]GC[AT]^C BsmAI 205 GAGAC GTCTC(1/5) Bsp1407I 1010 TGTACA T^GTACA BspEI 387 TCCGGA T^CCGGA BsrDI 350 CATTGC GCAATG(2/0) BsrGI 1010 TGTACA T^GTACA BstXI 94 CCAGTAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 357 GCGC GCG^C DraI 268 TTTAAA TTT^AAA DraII 928 GGGCCCT [GA]G^G[ACGT]CC[CT] EaeI 666 TGGCCA [CT]^GGCC[GA] EarI 1096 GAAGAG CTCTTC(1/4) EcoO109I 928 GGGCCCT [GA]G^G[ACGT]CC[CT] EcoT14I 1080 CCAAGG C^C[AT][AT]GG HaeII 356 AGCGCC [GA]GCGC^[CT] HapII 388 CCGG C^CGG HhaI 357 GCGC GCG^C HinP1I 357 GCGC G^CGC HincII 280 GTCAAC GT[CT]^[GA]AC HindII 280 GTCAAC GT[CT]^[GA]AC HpaII 388 CCGG C^CGG Ksp632I 1096 GAAGAG CTCTTC(1/4) MaeIII 586 GTAAC ^GT[ACGT]AC MamI 979 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 666 TGGCCA TGG^CCA MroI 387 TCCGGA T^CCGGA MscI 666 TGGCCA TGG^CCA MslI 285 CATTCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 388 CCGG C^CGG NdeI 527 CATATG CA^TATG NspI 1333 GCATGC [GA]CATG^[CT] SmlI 555 CTTGAG C^T[CT][GA]AG SphI 1333 GCATGC GCATG^C SspBI 1010 TGTACA T^GTACA StyI 1080 CCAAGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccI 404 GTAGAC GT^[AC][GT]AC 855 GTCTAC AcsI 744 AAATTT [GA]^AATT[CT] 817 GAATTT AfaI 738 GTAC GT^AC 1011 GTAC ApoI 744 AAATTT [GA]^AATT[CT] 817 GAATTT Asp700I 1047 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1068 GAAAGACTTC AvaII 1035 GGACC G^G[AT]CC 1084 GGACC BmyI 928 GGGCCC G[AGT]GC[ACT]^C 1144 GTGCAC Bsp1286I 928 GGGCCC G[AGT]GC[ACT]^C 1144 GTGCAC Cac8I 1164 GCCAGC GC[ACGT]^[ACGT]GC 1333 GCATGC MspA1I 643 CAGCTG C[AC]G^C[GT]G 1108 CAGCTG PvuII 643 CAGCTG CAG^CTG 1108 CAGCTG RsaI 738 GTAC GT^AC 1011 GTAC SexAI 135 ACCTGGT A^CC[AT]GGT 1349 ACCTGGT SfcI 857 CTACAG C^T[GA][CT]AG 1175 CTATAG SspI 432 AATATT AAT^ATT 724 AATATT TaqI 633 TCGA T^CGA 795 TCGA TthHB8I 633 TCGA T^CGA 795 TCGA XmnI 1047 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1068 GAAAGACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AluI AlwI BbvI BpmI BsaJI BsiYI BslI BspHI BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeII MboI MboII MflI MnlI MseI MvaI NdeII NlaIII NlaIV RcaI Sau3AI Sau96I ScrFI SfaNI TfiI Tru9I TseI Tsp509I TspRI XhoII