(Circular) (Six-base) MAPSORT of: pFN21AE1953.seq Check: 124 from: 1 to: 6232 pFN21AE1953 6232 bp With 182 enzymes: SgfI * August 16, 2010 14:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4042 278 Size: 53 83 186 936 2506 2468 Fragments arranged by size: 2506 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2410 2861 3575 1937 Size: 473 451 714 4594 Fragments arranged by size: 4594 714 473 451 Acc65I G'GTAC_C Cuts at: 3553 3553 Size: 6232 AclI AA'CG_TT Cuts at: 1563 4359 4732 1563 Size: 2796 373 3063 Fragments arranged by size: 3063 2796 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2580 2598 3510 4295 5679 5947 1632 Size: 948 18 912 785 1384 268 1917 Fragments arranged by size: 1917 1384 948 912 785 268 18 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6035 AflIII A'CryG_T Cuts at: 1203 2377 5131 6108 1203 Size: 1174 2754 977 1327 Fragments arranged by size: 2754 1327 1174 977 AgeI A'CCGG_T Cuts at: 5063 5063 Size: 6232 AhdI GACnn_n'nnGTC Cuts at: 4961 4961 Size: 6232 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6232 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6232 AlwNI CAG_nnn'CTG Cuts at: 3118 3975 5547 3118 Size: 857 1572 3803 Fragments arranged by size: 3803 1572 857 ApaI G_GGCC'C Cuts at: 2937 2937 Size: 6232 ApaLI G'TGCA_C Cuts at: 3149 4289 5445 3149 Size: 1140 1156 3936 Fragments arranged by size: 3936 1156 1140 ApoI r'AATT_y Cuts at: 1493 2749 2822 3541 3767 3919 6028 1493 Size: 1256 73 719 226 152 2109 1697 Fragments arranged by size: 2109 1697 1256 719 226 152 73 AseI AT'TA_AT Cuts at: 160 1032 4786 160 Size: 872 3754 1606 Fragments arranged by size: 3754 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 AvaI C'yCGr_G Cuts at: 1870 1943 3557 1870 Size: 73 1614 4545 Fragments arranged by size: 4545 1614 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2860 2893 2860 Size: 33 6199 BamHI G'GATC_C Cuts at: 2425 3562 3929 2425 Size: 1137 367 4728 Fragments arranged by size: 4728 1137 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3553 5008 618 Size: 325 217 627 1766 1455 1842 Fragments arranged by size: 1842 1766 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2937 3551 1391 Size: 1546 614 4072 Fragments arranged by size: 4072 1546 614 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3049 928 Size: 971 1150 4111 Fragments arranged by size: 4111 1150 971 BbvCI CC'TCA_GC Cuts at: 2707 2707 Size: 6232 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4423 4457 1903 Size: 34 2486 34 3678 Fragments arranged by size: 3678 2486 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4124 5334 4124 Size: 1210 5022 BclI T'GATC_A Cuts at: 1548 1548 Size: 6232 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4843 136 Size: 107 122 71 631 605 3171 1525 Fragments arranged by size: 3171 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2505 6227 2505 Size: 3722 2510 BlpI GC'TnA_GC Cuts at: 3649 3649 Size: 6232 Bme1580I G_kGCm'C Cuts at: 1790 2937 3153 4293 5449 1790 Size: 1147 216 1140 1156 2573 Fragments arranged by size: 2573 1156 1147 1140 216 BmgBI CAC'GTC Cuts at: 1318 2378 1318 Size: 1060 5172 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3963 4921 442 Size: 416 53 83 399 24 2546 958 1753 Fragments arranged by size: 2546 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6232 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2793 3330 3353 4892 1239 Size: 1554 537 23 1539 2579 Fragments arranged by size: 2579 1554 1539 537 23 Bpu10I CC'TnA_GC Cuts at: 2707 2707 Size: 6232 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2581 4357 5222 5520 2581 Size: 1776 865 298 3293 Fragments arranged by size: 3293 1776 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4895 882 Size: 617 3396 2219 Fragments arranged by size: 3396 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4023 493 Size: 711 2819 2702 Fragments arranged by size: 2819 2702 711 BsaBI GATnn'nnATC Cuts at: 2988 6226 2988 Size: 3238 2994 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4039 4421 5056 275 Size: 53 83 186 936 2506 382 635 1451 Fragments arranged by size: 2506 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2392 4665 5063 5337 5484 5895 2392 Size: 2273 398 274 147 411 2729 Fragments arranged by size: 2729 2273 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6007 Fragments arranged by size: 6007 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6232 BseYI C'CCAG_C Cuts at: 2924 3118 5435 5931 2924 Size: 194 2317 496 3225 Fragments arranged by size: 3225 2317 496 194 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2950 1163 Size: 573 1214 4445 Fragments arranged by size: 4445 1214 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3682 4443 5471 1540 Size: 275 186 1681 761 1028 2301 Fragments arranged by size: 2301 1681 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3153 4293 4378 5449 3153 Size: 1140 85 1071 3936 Fragments arranged by size: 3936 1140 1071 85 BsmI GAATG_Cn' Cuts at: 3743 3836 3743 Size: 93 6139 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2937 3153 3551 4293 4378 5449 1391 Size: 399 1147 216 398 742 85 1071 2174 Fragments arranged by size: 2174 1147 1071 742 399 398 216 85 BspEI T'CCGG_A Cuts at: 2392 5895 2392 Size: 3503 2729 BspHI T'CATG_A Cuts at: 2446 3159 3454 4121 5851 2446 Size: 713 295 667 1730 2827 Fragments arranged by size: 2827 1730 713 667 295 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3587 844 Size: 2743 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4119 1394 Size: 429 2296 3507 Fragments arranged by size: 3507 2296 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2354 4727 4901 65 Size: 1183 1106 2373 174 1396 Fragments arranged by size: 2373 1396 1183 1106 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4876 5063 1700 Size: 110 3066 187 2869 Fragments arranged by size: 3066 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3015 96 Size: 2919 3313 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6232 BssSI C'ACGA_G Cuts at: 4292 5304 4292 Size: 1012 5220 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6232 BstBI TT'CG_AA Cuts at: 5036 5036 Size: 6232 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6232 BstXI CCAn_nnnn'nTGG Cuts at: 2106 2106 Size: 6232 BstYI r'GATC_y Cuts at: 1972 2016 2389 2425 2505 3460 3562 3929 4314 Size: 44 373 36 80 955 102 367 385 Cuts at: 4314 4331 5783 5869 5881 6227 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 955 385 373 367 346 102 86 80 44 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4741 Fragments arranged by size: 4741 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5493 Fragments arranged by size: 5493 683 56 BtsI GCAGTG_nn' Cuts at: 3238 3748 4542 4562 3238 Size: 510 794 20 4908 Fragments arranged by size: 4908 794 510 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6232 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6197 DraI TTT'AAA Cuts at: 2275 3536 2275 Size: 1261 4971 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5239 809 Size: 507 3923 1802 Fragments arranged by size: 3923 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2671 3679 4568 Size: 54 1425 50 275 18 841 1008 889 Cuts at: 4568 6197 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1008 889 841 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3679 1537 Size: 275 1867 4090 Fragments arranged by size: 4090 1867 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3096 4162 1353 Size: 1743 1066 3423 Fragments arranged by size: 3423 1743 1066 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4819 5193 5339 387 Size: 1064 138 3230 374 146 1280 Fragments arranged by size: 3230 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2580 2598 2793 3330 3353 3510 4295 Size: 393 948 18 195 537 23 157 785 Cuts at: 4295 4892 5679 5947 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 948 787 785 597 537 393 268 195 157 23 18 EcoO109I rG'GnC_Cy Cuts at: 2934 2934 Size: 6232 EcoRI G'AATT_C Cuts at: 3541 3919 3541 Size: 378 5854 FspI TGC'GCA Cuts at: 4738 4738 Size: 6232 HaeII r_GCGC'y Cuts at: 1678 2365 5379 1678 Size: 687 3014 2531 Fragments arranged by size: 3014 2531 687 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2374 2406 3427 3459 4873 Size: 32 135 32 802 32 1021 32 1414 Cuts at: 4873 4905 4947 4979 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1414 1021 802 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2287 3576 3822 669 Size: 988 281 349 1289 246 3079 Fragments arranged by size: 3079 1289 988 349 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6232 HpaI GTT'AAC Cuts at: 3822 3822 Size: 6232 KpnI G_GTAC'C Cuts at: 3557 3557 Size: 6232 MfeI C'AATT_G Cuts at: 3831 3831 Size: 6232 MluI A'CGCG_T Cuts at: 6108 6108 Size: 6232 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2403 5346 5530 1305 Size: 1098 2943 184 2007 Fragments arranged by size: 2943 2007 1098 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2673 10 Size: 54 1425 343 841 3569 Fragments arranged by size: 3569 1425 841 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2294 4190 4549 4708 518 Size: 648 1128 1896 359 159 2042 Fragments arranged by size: 2042 1896 1128 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2650 3115 3648 4325 5473 5718 1606 Size: 1044 465 533 677 1148 245 2120 Fragments arranged by size: 2120 1148 1044 677 533 465 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4741 Fragments arranged by size: 4741 939 552 NdeI CA'TA_TG Cuts at: 387 2533 387 Size: 2146 4086 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6232 NotI GC'GGCC_GC Cuts at: 3679 3679 Size: 6232 NspI r_CATG'y Cuts at: 1132 1229 3342 3590 5135 1132 Size: 97 2113 248 1545 2229 Fragments arranged by size: 2229 2113 1545 248 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6232 PciI A'CATG_T Cuts at: 5131 5131 Size: 6232 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6232 PfoI T'CCnGG_A Cuts at: 2695 2695 Size: 6232 PmeI GTTT'AAAC Cuts at: 3536 3536 Size: 6232 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4301 4333 5814 5846 4301 Size: 32 1481 32 4687 Fragments arranged by size: 4687 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6232 PsiI TTA'TAA Cuts at: 3802 3802 Size: 6232 PspOMI G'GGCC_C Cuts at: 2933 2933 Size: 6232 PstI C_TGCA'G Cuts at: 830 3584 830 Size: 2754 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6232 PvuII CAG'CTG Cuts at: 2650 3115 2650 Size: 465 5767 SalI G'TCGA_C Cuts at: 1936 3574 1936 Size: 1638 4594 SbfI CC_TGCA'GG Cuts at: 3584 3584 Size: 6232 ScaI AGT'ACT Cuts at: 4480 6192 4480 Size: 1712 4520 SexAI A'CCwGG_T Cuts at: 2140 3354 2140 Size: 1214 5018 SfcI C'TryA_G Cuts at: 826 1045 2862 3180 3580 4715 5396 5587 826 Size: 219 1817 318 400 1135 681 191 1471 Fragments arranged by size: 1817 1471 1135 681 400 318 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6232 SmaI CCC'GGG Cuts at: 1872 3559 1872 Size: 1687 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2560 4336 5237 5499 820 Size: 197 926 617 1776 901 262 1553 Fragments arranged by size: 1776 1553 926 901 617 262 197 SnaBI TAC'GTA Cuts at: 493 4023 493 Size: 3530 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6232 SphI G_CATG'C Cuts at: 1132 3342 3590 1132 Size: 2210 248 3774 Fragments arranged by size: 3774 2210 248 SspI AAT'ATT Cuts at: 5 52 2439 2731 4156 5 Size: 47 2387 292 1425 2081 Fragments arranged by size: 2387 2081 1425 292 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6232 StyI C'CwwG_G Cuts at: 513 1065 2004 3085 3671 513 Size: 552 939 1081 586 3074 Fragments arranged by size: 3074 1081 939 586 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4259 4276 4429 4614 5059 325 Size: 1255 2679 17 153 185 445 1498 Fragments arranged by size: 2679 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3015 4478 6190 Size: 274 80 33 51 1443 1038 1463 1712 Cuts at: 6190 96 Size: 138 Fragments arranged by size: 1712 1463 1443 1038 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3374 3406 6192 6224 3374 Size: 32 2786 32 3382 Fragments arranged by size: 3382 2786 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6232 XbaI T'CTAG_A Cuts at: 3568 3568 Size: 6232 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6232 XmaI C'CCGG_G Cuts at: 1870 3557 1870 Size: 1687 4545 XmnI GAAnn'nnTTC Cuts at: 3056 3077 4361 3056 Size: 21 1284 4927 Fragments arranged by size: 4927 1284 21 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4040 276 Size: 53 83 186 936 2506 2468 Fragments arranged by size: 2506 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI