################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AclI AcsI AgeI ApoI AscI AseI AsnI Asp700I AspI AvrII BbrPI BbsI BciVI BlnI BpuAI BsaBI BsiWI BsmI BspDI Bst1107I BstBI BstEII BstPI BstZ17I ClaI DraI DrdI EcoO65I EcoRI EcoRV EcoT22I FseI HincII HindII HindIII HpaI MamI MfeI MluI MunI NcoI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I SalI ScaI SfuI SgrAI SnaBI SpeI SphI SspI SwaI Tsp509I Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 1792 GACGTC GACGT^C Acc65I 1415 GGTACC G^GTACC AccI 142 GTCTAC GT^[AC][GT]AC AccIII 2952 TCCGGA T^CCGGA AflII 2152 CTTAAG C^TTAAG AflIII 1085 ACATGT A^C[GA][CT]GT AhdI 2491 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2302 GTGCAC G^TGCAC AocI 2842 CCTCAGG CC^T[ACGT]AGG ApaLI 2302 GTGCAC G^TGCAC Asp718I 1415 GGTACC G^GTACC AspEI 2491 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 2525 TGGCCA TGG^CCA BamHI 3838 GGATCC G^GATCC BbvCI 3195 GCTGAGG CCTCAGC(-5/-2) BclI 2333 TGATCA T^GATCA BfrI 2152 CTTAAG C^TTAAG BglII 2156 AGATCT A^GATCT BseAI 2952 TCCGGA T^CCGGA Bsp1407I 2591 TGTACA T^GTACA BspEI 2952 TCCGGA T^CCGGA BspHI 830 TCATGA T^CATGA BspLU11I 1085 ACATGT A^CATGT BsrDI 1206 CATTGC GCAATG(2/0) BsrGI 2591 TGTACA T^GTACA Bsu36I 2842 CCTCAGG CC^T[ACGT]AGG CpoI 19 CGGTCCG CG^G[AT]CCG Eam1105I 2491 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2842 CCTCAGG CC^T[ACGT]AGG FbaI 2333 TGATCA T^GATCA KpnI 1415 GGTACC GGTAC^C MluNI 2525 TGGCCA TGG^CCA MroI 2952 TCCGGA T^CCGGA MscI 2525 TGGCCA TGG^CCA MseI 2153 TTAA T^TAA NheI 2134 GCTAGC G^CTAGC NotI 255 GCGGCCGC GC^GGCCGC PshAI 3609 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 1998 CGATCG CGAT^CG RcaI 830 TCATGA T^CATGA RsrII 19 CGGTCCG CG^G[AT]CCG SexAI 1418 ACCTGGT A^CC[AT]GGT SfiI 3003 GGCCATGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SspBI 2591 TGTACA T^GTACA Tru9I 2153 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AviII 272 TGCGCA TGC^GCA 2015 TGCGCA BcgI 2922 CGAGGAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2937 CGAGGAGCCTGC BsaAI 145 TACGTG [CT]AC^GT[GA] 1971 CACGTA BssSI 2007 CACGAG CACGAG(-5/-1) 2548 CACGAG DraIII 1493 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG 2296 CACATCGTG EcoT14I 396 CCTTGG C^C[AT][AT]GG 3210 CCTTGG FspI 272 TGCGCA TGC^GCA 2015 TGCGCA NdeI 1410 CATATG CA^TATG 1722 CATATG SacI 1240 GAGCTC GAGCT^C 2551 GAGCTC SapI 3109 GAAGAGC GCTCTTC(1/4) 2083 GCTCTTC SbfI 465 CCTGCAGG CCTGCA^GG 2265 CCTGCAGG Sse8387I 465 CCTGCAGG CCTGCA^GG 2265 CCTGCAGG StyI 396 CCTTGG C^C[AT][AT]GG 3210 CCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 135 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI Aor51HI ApaI AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssHII BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV NspI PaeR7I PflMI PleI PpuMI PstI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI StuI TaqI TfiI TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoI XhoII XmaCI XmaI