(Circular) (Six-base) MAPSORT of: pFN21AE2101.seq Check: 2310 from: 1 to: 7291 pFN21AE2101 7291 bp With 182 enzymes: SgfI * August 16, 2010 14:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5101 278 Size: 53 83 186 936 3565 2468 Fragments arranged by size: 3565 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2677 4634 1937 Size: 740 1957 4594 Fragments arranged by size: 4594 1957 740 Acc65I G'GTAC_C Cuts at: 4612 4612 Size: 7291 AclI AA'CG_TT Cuts at: 1563 5418 5791 1563 Size: 3855 373 3063 Fragments arranged by size: 3855 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2048 2463 2574 2796 3345 3568 4559 5354 Size: 416 415 111 222 549 223 991 795 Cuts at: 5354 6738 7006 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 991 795 549 416 415 268 223 222 111 AflII C'TTAA_G Cuts at: 820 1017 2090 820 Size: 197 1073 6021 Fragments arranged by size: 6021 1073 197 AflIII A'CryG_T Cuts at: 1203 2468 6190 7167 1203 Size: 1265 3722 977 1327 Fragments arranged by size: 3722 1327 1265 977 AgeI A'CCGG_T Cuts at: 6122 6122 Size: 7291 AhdI GACnn_n'nnGTC Cuts at: 6020 6020 Size: 7291 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7291 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4284 1184 Size: 3100 4191 AlwNI CAG_nnn'CTG Cuts at: 2269 2567 2687 3505 5034 6606 2269 Size: 298 120 818 1529 1572 2954 Fragments arranged by size: 2954 1572 1529 818 298 120 ApaLI G'TGCA_C Cuts at: 5348 6504 5348 Size: 1156 6135 ApoI r'AATT_y Cuts at: 1493 3179 3357 3839 3929 4011 4170 4439 4600 Size: 1686 178 482 90 82 159 269 161 Cuts at: 4600 4826 4978 7087 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1686 482 269 226 178 161 159 152 90 82 AseI AT'TA_AT Cuts at: 160 1032 2795 5845 160 Size: 872 1763 3050 1606 Fragments arranged by size: 3050 1763 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7291 AvaI C'yCGr_G Cuts at: 1870 1943 4122 4616 1870 Size: 73 2179 494 4545 Fragments arranged by size: 4545 2179 494 73 AvrII C'CTAG_G Cuts at: 2831 2831 Size: 7291 BamHI G'GATC_C Cuts at: 4621 4988 4621 Size: 367 6924 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4612 6067 618 Size: 325 217 627 2825 1455 1842 Fragments arranged by size: 2825 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4610 1391 Size: 3219 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2168 3288 3610 928 Size: 971 269 1120 322 4609 Fragments arranged by size: 4609 1120 971 322 269 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5482 5516 1903 Size: 34 3545 34 3678 Fragments arranged by size: 3678 3545 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5183 6393 5183 Size: 1210 6081 BclI T'GATC_A Cuts at: 1548 1548 Size: 7291 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5902 136 Size: 107 122 71 631 605 4230 1525 Fragments arranged by size: 4230 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3595 7286 3595 Size: 3691 3600 BlpI GC'TnA_GC Cuts at: 4708 4708 Size: 7291 Bme1580I G_kGCm'C Cuts at: 1790 5352 6508 1790 Size: 3562 1156 2573 Fragments arranged by size: 3562 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7291 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5022 5980 442 Size: 416 53 83 399 24 3605 958 1753 Fragments arranged by size: 3605 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7291 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4137 4137 Size: 7291 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2488 2838 5951 1239 Size: 1249 350 3113 2579 Fragments arranged by size: 3113 2579 1249 350 Bpu10I CC'TnA_GC Cuts at: 2824 2824 Size: 7291 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2838 3887 5416 6281 6579 2838 Size: 1049 1529 865 298 3550 Fragments arranged by size: 3550 1529 1049 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5954 882 Size: 617 4455 2219 Fragments arranged by size: 4455 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2131 2675 5082 493 Size: 711 927 544 2407 2702 Fragments arranged by size: 2702 2407 927 711 544 BsaBI GATnn'nnATC Cuts at: 7285 7285 Size: 7291 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5098 5480 6115 275 Size: 53 83 186 936 3565 382 635 1451 Fragments arranged by size: 3565 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3063 3486 5724 6122 6396 6543 6954 3063 Size: 423 2238 398 274 147 411 3400 Fragments arranged by size: 3400 2238 423 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2572 2602 2842 2872 1178 Size: 30 165 30 1169 30 240 30 5597 Fragments arranged by size: 5597 1169 240 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7291 BseYI C'CCAG_C Cuts at: 2970 4179 6494 6990 2970 Size: 1209 2315 496 3271 Fragments arranged by size: 3271 2315 1209 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6718 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4741 5502 6530 1540 Size: 275 186 2740 761 1028 2301 Fragments arranged by size: 2740 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2534 5352 5437 6508 2534 Size: 2818 85 1071 3317 Fragments arranged by size: 3317 2818 1071 85 BsmI GAATG_Cn' Cuts at: 4239 4802 4895 4239 Size: 563 93 6635 Fragments arranged by size: 6635 563 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2534 4610 5352 5437 6508 1391 Size: 399 744 2076 742 85 1071 2174 Fragments arranged by size: 2174 2076 1071 744 742 399 85 BspEI T'CCGG_A Cuts at: 3063 6954 3063 Size: 3891 3400 BspHI T'CATG_A Cuts at: 4033 5180 6910 4033 Size: 1147 1730 4414 Fragments arranged by size: 4414 1730 1147 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4391 4518 4646 844 Size: 3547 127 128 3489 Fragments arranged by size: 3547 3489 128 127 BsrBI CCG'CTC Cuts at: 1394 1823 5178 1394 Size: 429 3355 3507 Fragments arranged by size: 3507 3355 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3117 3197 3238 4432 5786 5960 65 Size: 1183 1869 80 41 1194 1354 174 1396 Fragments arranged by size: 1869 1396 1354 1194 1183 174 80 41 BsrFI r'CCGG_y Cuts at: 1700 1810 5935 6122 1700 Size: 110 4125 187 2869 Fragments arranged by size: 4125 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7291 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7291 BssSI C'ACGA_G Cuts at: 5351 6363 5351 Size: 1012 6279 BstAPI GCAn_nnn'nTGC Cuts at: 794 4189 794 Size: 3395 3896 BstBI TT'CG_AA Cuts at: 6095 6095 Size: 7291 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7291 BstXI CCAn_nnnn'nTGG Cuts at: 2874 3210 2874 Size: 336 6955 BstYI r'GATC_y Cuts at: 1972 2507 3403 3595 4621 4988 5373 5390 6842 Size: 535 896 192 1026 367 385 17 1452 Cuts at: 6842 6928 6940 7286 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1026 896 535 385 367 346 192 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3819 3832 513 Size: 552 2754 13 3972 Fragments arranged by size: 3972 2754 552 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6552 Fragments arranged by size: 6552 683 56 BtsI GCAGTG_nn' Cuts at: 4807 5601 5621 4807 Size: 794 20 6477 Fragments arranged by size: 6477 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7291 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4292 4327 529 Size: 35 3728 35 3493 Fragments arranged by size: 3728 3493 35 35 DraI TTT'AAA Cuts at: 2626 4595 2626 Size: 1969 5322 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6298 809 Size: 507 4982 1802 Fragments arranged by size: 4982 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3507 4738 5627 Size: 54 1425 50 275 18 1677 1231 889 Cuts at: 5627 7256 8 Size: 1629 43 Fragments arranged by size: 1677 1629 1425 1231 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4738 1537 Size: 275 2926 4090 Fragments arranged by size: 4090 2926 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3212 4343 5221 1353 Size: 1859 1131 878 3423 Fragments arranged by size: 3423 1859 1131 878 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5878 6252 6398 387 Size: 1064 138 4289 374 146 1280 Fragments arranged by size: 4289 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2048 2463 2488 2574 2796 2838 3345 Size: 393 416 415 25 86 222 42 507 Cuts at: 3345 3568 4559 5354 5951 6738 7006 1239 Size: 223 991 795 597 787 268 1524 Fragments arranged by size: 1524 991 795 787 597 507 416 415 393 268 223 222 86 42 25 EcoO109I rG'GnC_Cy Cuts at: 2828 4219 2828 Size: 1391 5900 EcoRI G'AATT_C Cuts at: 3357 4600 4978 3357 Size: 1243 378 5670 Fragments arranged by size: 5670 1243 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3898 3898 Size: 7291 FspI TGC'GCA Cuts at: 5797 5797 Size: 7291 HaeII r_GCGC'y Cuts at: 1678 6438 1678 Size: 4760 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2003 2035 2138 2170 2486 Size: 32 135 32 431 32 103 32 316 Cuts at: 2486 2518 3571 3603 3899 3931 4021 4053 4257 Size: 32 1053 32 296 32 90 32 204 Cuts at: 4257 4289 5932 5964 6006 6038 1373 Size: 32 1643 32 42 32 2626 Fragments arranged by size: 2626 1643 1053 431 316 296 204 135 103 90 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2986 3013 3253 4003 4635 4881 Size: 988 281 1048 27 240 750 632 246 Cuts at: 4881 669 Size: 3079 Fragments arranged by size: 3079 1048 988 750 632 281 246 240 27 HindIII A'AGCT_T Cuts at: 748 4146 748 Size: 3398 3893 HpaI GTT'AAC Cuts at: 4881 4881 Size: 7291 KpnI G_GTAC'C Cuts at: 4616 4616 Size: 7291 MfeI C'AATT_G Cuts at: 4890 4890 Size: 7291 MluI A'CGCG_T Cuts at: 7167 7167 Size: 7291 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3877 6405 6589 1305 Size: 2572 2528 184 2007 Fragments arranged by size: 2572 2528 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3509 10 Size: 54 1425 343 1677 3792 Fragments arranged by size: 3792 1677 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2247 3208 4097 5249 5608 5767 518 Size: 648 1081 961 889 1152 359 159 2042 Fragments arranged by size: 2042 1152 1081 961 889 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4707 5384 6532 6777 1606 Size: 3101 677 1148 245 2120 Fragments arranged by size: 3101 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 3819 3832 513 Size: 552 2754 13 3972 Fragments arranged by size: 3972 2754 552 13 NdeI CA'TA_TG Cuts at: 387 387 Size: 7291 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7291 NotI GC'GGCC_GC Cuts at: 4738 4738 Size: 7291 NspI r_CATG'y Cuts at: 1132 1229 2472 4271 4649 6194 1132 Size: 97 1243 1799 378 1545 2229 Fragments arranged by size: 2229 1799 1545 1243 378 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7291 PciI A'CATG_T Cuts at: 2468 6190 2468 Size: 3722 3569 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7291 PfoI T'CCnGG_A Cuts at: 3625 3625 Size: 7291 PmeI GTTT'AAAC Cuts at: 4595 4595 Size: 7291 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5360 5392 6873 6905 5360 Size: 32 1481 32 5746 Fragments arranged by size: 5746 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4219 4219 Size: 7291 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7291 PsiI TTA'TAA Cuts at: 4861 4861 Size: 7291 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3473 3505 3473 Size: 32 7259 PstI C_TGCA'G Cuts at: 830 4368 4643 830 Size: 3538 275 3478 Fragments arranged by size: 3538 3478 275 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7291 SalI G'TCGA_C Cuts at: 1936 4633 1936 Size: 2697 4594 SbfI CC_TGCA'GG Cuts at: 4643 4643 Size: 7291 ScaI AGT'ACT Cuts at: 5539 7251 5539 Size: 1712 5579 SexAI A'CCwGG_T Cuts at: 2869 2869 Size: 7291 SfcI C'TryA_G Cuts at: 826 1045 2429 3086 4364 4639 5774 6455 6646 Size: 219 1384 657 1278 275 1135 681 191 Cuts at: 6646 826 Size: 1471 Fragments arranged by size: 1471 1384 1278 1135 681 657 275 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7291 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7291 SmaI CCC'GGG Cuts at: 1872 4618 1872 Size: 2746 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2090 2853 3902 4122 5395 6296 Size: 197 926 147 763 1049 220 1273 901 Cuts at: 6296 6558 820 Size: 262 1553 Fragments arranged by size: 1553 1273 1049 926 901 763 262 220 197 147 SnaBI TAC'GTA Cuts at: 493 2131 5082 493 Size: 1638 2951 2702 Fragments arranged by size: 2951 2702 1638 SpeI A'CTAG_T Cuts at: 152 152 Size: 7291 SphI G_CATG'C Cuts at: 1132 4649 1132 Size: 3517 3774 SspI AAT'ATT Cuts at: 5 52 3791 3967 4060 4187 5215 5 Size: 47 3739 176 93 127 1028 2081 Fragments arranged by size: 3739 2081 1028 176 127 93 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7291 StyI C'CwwG_G Cuts at: 513 1065 2511 2831 3819 3832 4730 513 Size: 552 1446 320 988 13 898 3074 Fragments arranged by size: 3074 1446 988 898 552 320 13 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2680 3604 5318 5335 5488 5673 6118 Size: 1255 1100 924 1714 17 153 185 445 Cuts at: 6118 325 Size: 1498 Fragments arranged by size: 1714 1498 1255 1100 924 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2732 3990 5537 Size: 274 80 33 51 1443 755 1258 1547 Cuts at: 5537 7249 96 Size: 1712 138 Fragments arranged by size: 1712 1547 1443 1258 755 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2173 2205 2572 2604 7251 7283 2173 Size: 32 367 32 4647 32 2181 Fragments arranged by size: 4647 2181 367 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2121 1316 Size: 805 6486 XbaI T'CTAG_A Cuts at: 2633 4627 2633 Size: 1994 5297 XhoI C'TCGA_G Cuts at: 1943 4122 1943 Size: 2179 5112 XmaI C'CCGG_G Cuts at: 1870 4616 1870 Size: 2746 4545 XmnI GAAnn'nnTTC Cuts at: 4173 5420 4173 Size: 1247 6044 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5099 276 Size: 53 83 186 936 3565 2468 Fragments arranged by size: 3565 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI