(Linear) (Six-base) MAPSORT of: KIEE2101.seq Check: 488 from: 1 to: 2586 KIEE2101.orf With 182 enzymes: SgfI * July 15, 2010 18:54 .. AccI GT'mk_AC Cuts at: 0 672 2586 Size: 672 1914 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 43 458 569 791 1340 1563 2554 2586 Size: 43 415 111 222 549 223 991 32 Fragments arranged by size: 991 549 415 223 222 111 43 32 AflII C'TTAA_G Cuts at: 0 85 2586 Size: 85 2501 AflIII A'CryG_T Cuts at: 0 463 2586 Size: 463 2123 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2279 2586 Size: 2279 307 AlwNI CAG_nnn'CTG Cuts at: 0 264 562 682 1500 2586 Size: 264 298 120 818 1086 Fragments arranged by size: 1086 818 298 264 120 ApoI r'AATT_y Cuts at: 0 1174 1352 1834 1924 2006 2165 2434 2586 Size: 1174 178 482 90 82 159 269 152 Fragments arranged by size: 1174 482 269 178 159 152 90 82 AseI AT'TA_AT Cuts at: 0 790 2586 Size: 790 1796 AvaI C'yCGr_G Cuts at: 0 2117 2586 Size: 2117 469 AvrII C'CTAG_G Cuts at: 0 826 2586 Size: 826 1760 BbsI GAAGACnn'nnnn_ Cuts at: 0 163 1283 1605 2586 Size: 163 1120 322 981 Fragments arranged by size: 1120 981 322 163 BglII A'GATC_T Cuts at: 0 1590 2586 Size: 1590 996 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2132 2586 Size: 2132 454 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 483 833 2586 Size: 483 350 1753 Fragments arranged by size: 1753 483 350 Bpu10I CC'TnA_GC Cuts at: 0 819 2586 Size: 819 1767 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 833 1882 2586 Size: 833 1049 704 Fragments arranged by size: 1049 833 704 BsaAI yAC'GTr Cuts at: 0 126 670 2586 Size: 126 544 1916 Fragments arranged by size: 1916 544 126 BsaWI w'CCGG_w Cuts at: 0 1058 1481 2586 Size: 1058 423 1105 Fragments arranged by size: 1105 1058 423 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 567 597 837 867 2586 Size: 567 30 240 30 1719 Fragments arranged by size: 1719 567 240 30 30 BseYI C'CCAG_C Cuts at: 0 965 2174 2586 Size: 965 1209 412 Fragments arranged by size: 1209 965 412 BsiHKAI G_wGCw'C Cuts at: 0 529 2586 Size: 529 2057 BsmI GAATG_Cn' Cuts at: 0 2234 2586 Size: 2234 352 Bsp1286I G_dGCh'C Cuts at: 0 529 2586 Size: 529 2057 BspEI T'CCGG_A Cuts at: 0 1058 2586 Size: 1058 1528 BspHI T'CATG_A Cuts at: 0 2028 2586 Size: 2028 558 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2386 2513 2586 Size: 2386 127 73 Fragments arranged by size: 2386 127 73 BsrDI GCAATG_nn' Cuts at: 0 1112 1192 1233 2427 2586 Size: 1112 80 41 1194 159 Fragments arranged by size: 1194 1112 159 80 41 BstAPI GCAn_nnn'nTGC Cuts at: 0 2184 2586 Size: 2184 402 BstXI CCAn_nnnn'nTGG Cuts at: 0 869 1205 2586 Size: 869 336 1381 Fragments arranged by size: 1381 869 336 BstYI r'GATC_y Cuts at: 0 502 1398 1590 2586 Size: 502 896 192 996 Fragments arranged by size: 996 896 502 192 BtgI C'CryG_G Cuts at: 0 1814 1827 2586 Size: 1814 13 759 Fragments arranged by size: 1814 759 13 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2287 2322 2586 Size: 2287 35 264 Fragments arranged by size: 2287 264 35 DraI TTT'AAA Cuts at: 0 621 2586 Size: 621 1965 EaeI y'GGCC_r Cuts at: 0 1502 2586 Size: 1502 1084 EarI CTCTTCn'nnn_ Cuts at: 0 1207 2338 2586 Size: 1207 1131 248 Fragments arranged by size: 1207 1131 248 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 43 458 483 569 791 833 1340 1563 Size: 43 415 25 86 222 42 507 223 Cuts at: 1563 2554 2586 Size: 991 32 Fragments arranged by size: 991 507 415 223 222 86 43 42 32 25 EcoO109I rG'GnC_Cy Cuts at: 0 823 2214 2586 Size: 823 1391 372 Fragments arranged by size: 1391 823 372 EcoRI G'AATT_C Cuts at: 0 1352 2586 Size: 1352 1234 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1893 2586 Size: 1893 693 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 30 133 165 481 513 1566 1598 1894 Size: 30 103 32 316 32 1053 32 296 Cuts at: 1894 1926 2016 2048 2252 2284 2586 Size: 32 90 32 204 32 302 Fragments arranged by size: 1053 316 302 296 204 103 90 32 32 32 32 32 32 30 HincII GTy'rAC Cuts at: 0 981 1008 1248 1998 2586 Size: 981 27 240 750 588 Fragments arranged by size: 981 750 588 240 27 HindIII A'AGCT_T Cuts at: 0 2141 2586 Size: 2141 445 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1872 2586 Size: 1872 714 MscI TGG'CCA Cuts at: 0 1504 2586 Size: 1504 1082 MslI CAynn'nnrTG Cuts at: 0 242 1203 2092 2586 Size: 242 961 889 494 Fragments arranged by size: 961 889 494 242 NcoI C'CATG_G Cuts at: 0 1814 1827 2586 Size: 1814 13 759 Fragments arranged by size: 1814 759 13 NspI r_CATG'y Cuts at: 0 467 2266 2586 Size: 467 1799 320 Fragments arranged by size: 1799 467 320 PciI A'CATG_T Cuts at: 0 463 2586 Size: 463 2123 PfoI T'CCnGG_A Cuts at: 0 1620 2586 Size: 1620 966 PpuMI rG'GwC_Cy Cuts at: 0 2214 2586 Size: 2214 372 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1468 1500 2586 Size: 1468 32 1086 Fragments arranged by size: 1468 1086 32 PstI C_TGCA'G Cuts at: 0 2363 2586 Size: 2363 223 SexAI A'CCwGG_T Cuts at: 0 864 2586 Size: 864 1722 SfcI C'TryA_G Cuts at: 0 424 1081 2359 2586 Size: 424 657 1278 227 Fragments arranged by size: 1278 657 424 227 SmlI C'TyrA_G Cuts at: 0 85 848 1897 2117 2586 Size: 85 763 1049 220 469 Fragments arranged by size: 1049 763 469 220 85 SnaBI TAC'GTA Cuts at: 0 126 2586 Size: 126 2460 SspI AAT'ATT Cuts at: 0 1786 1962 2055 2182 2586 Size: 1786 176 93 127 404 Fragments arranged by size: 1786 404 176 127 93 StyI C'CwwG_G Cuts at: 0 506 826 1814 1827 2586 Size: 506 320 988 13 759 Fragments arranged by size: 988 759 506 320 13 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 675 1599 2586 Size: 675 924 987 Fragments arranged by size: 987 924 675 TatI w'GTAC_w Cuts at: 0 727 1985 2586 Size: 727 1258 601 Fragments arranged by size: 1258 727 601 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 168 200 567 599 2586 Size: 168 32 367 32 1987 Fragments arranged by size: 1987 367 168 32 32 Tth111I GACn'n_nGTC Cuts at: 0 116 2586 Size: 116 2470 XbaI T'CTAG_A Cuts at: 0 628 2586 Size: 628 1958 XhoI C'TCGA_G Cuts at: 0 2117 2586 Size: 2117 469 XmnI GAAnn'nnTTC Cuts at: 0 2168 2586 Size: 2168 418 Enzymes that do cut: AccI AcuI AflII AflIII AlfI AlwNI ApoI AseI AvaI AvrII BbsI BglII BplI BpmI Bpu10I BpuEI BsaAI BsaWI BsaXI BseYI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BstAPI BstXI BstYI BtgI CspCI DraI EaeI EarI Eco57MI EcoO109I EcoRI FalI Hin4I HincII HindIII MmeI MscI MslI NcoI NspI PciI PfoI PpuMI PsrI PstI SexAI SfcI SmlI SnaBI SspI StyI TaqII TatI TstI Tth111I XbaI XhoI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AgeI AhdI AleI AloI ApaI ApaLI AscI AsiSI BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BsaI BsaBI BsaHI BseRI BsgI BsiEI BsiWI BsmBI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SpeI SphI SrfI StuI SwaI XcmI XmaI ZraI