################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## AccII AclI AcsI AgeI AhdI ApoI AscI AseI AsnI AspEI AspI AvrII BbeI BbrPI BciVI BclI BglII BlnI BsaBI BsiWI Bsp1407I BspDI BspHI BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Cfr10I ClaI CpoI DraI DrdI Eam1105I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KasI KspI MamI MfeI MluI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspBI SspI StuI SwaI Tsp509I Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 817 GACGTC GACGT^C Acc65I 1379 GGTACC G^GTACC AccI 74 GTCTAC GT^[AC][GT]AC AflII 1279 CTTAAG C^TTAAG Alw44I 1423 GTGCAC G^TGCAC Aor51HI 1107 AGCGCT AGC^GCT ApaLI 1423 GTGCAC G^TGCAC Asp700I 1552 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1379 GGTACC G^GTACC BamHI 1826 GGATCC G^GATCC BanI 1379 GGTACC G^G[CT][GA]CC BbsI 1971 GAAGAC GAAGAC(2/6) BbvCI 2289 CCTCAGC CCTCAGC(-5/-2) BcgI 327 GCAGCAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 1562 CTAG C^TAG BfrI 1279 CTTAAG C^TTAAG BglI 26 GCCCCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 1971 GAAGAC GAAGAC(2/6) BsaAI 1471 TACGTG [CT]AC^GT[GA] BsaI 2254 GAGACC GGTCTC(1/5) BsiEI 2202 CGGCCG CG[GA][CT]^CG BsmI 1565 GCATTC GAATGC(1/-1) BspLU11I 149 ACATGT A^CATGT BsrBI 1045 GAGCGG CCGCTC(-3/-3) BssSI 646 CACGAG CACGAG(-5/-1) BstXI 959 CCACCGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 824 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 2202 CGGCCG C^GGCCG EclXI 2202 CGGCCG C^GGCCG Eco47III 1107 AGCGCT AGC^GCT Eco52I 2202 CGGCCG C^GGCCG HaeII 1107 AGCGCT [GA]GCGC^[CT] KpnI 1379 GGTACC GGTAC^C MaeI 1562 CTAG C^TAG NspI 149 ACATGT [GA]CATG^[CT] PflMI 1518 CCAGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SapI 414 GAAGAGC GCTCTTC(1/4) SexAI 1679 ACCTGGT A^CC[AT]GGT SmaI 1040 CCCGGG CCC^GGG TfiI 1298 GAATC G^A[AT]TC Van91I 1518 CCAGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 1040 CCCGGG C^CCGGG XmaI 1040 CCCGGG C^CCGGG XmnI 1552 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccIII 1013 TCCGGA T^CCGGA 1344 TCCGGA AcyI 817 GACGTC G[GA]^CG[CT]C 1733 GACGCC AflIII 149 ACATGT A^C[GA][CT]GT 2225 ACGTGT AviII 518 TGCGCA TGC^GCA 576 TGCGCA BalI 725 TGGCCA TGG^CCA 956 TGGCCA BlpI 303 GCTGAGC GC^T[ACGT]AGC 1411 GCTGAGC Bpu1102I 303 GCTGAGC GC^T[ACGT]AGC 1411 GCTGAGC BsaHI 817 GACGTC G[GA]^CG[CT]C 1733 GACGCC BsaWI 1013 TCCGGA [AT]^CCGG[AT] 1344 TCCGGA BseAI 1013 TCCGGA T^CCGGA 1344 TCCGGA BsmBI 1861 CGTCTC CGTCTC(1/5) 1780 GAGACG BspEI 1013 TCCGGA T^CCGGA 1344 TCCGGA BspMI 641 ACCTGC ACCTGC(4/8) 1101 GCAGGT CelII 303 GCTGAGC GC^T[ACGT]AGC 1411 GCTGAGC DsaI 36 CCACGG C^C[GA][CT]GG 1383 CCACGG EcoNI 642 CCTGCACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 705 CCTGGTGCAGG FspI 518 TGCGCA TGC^GCA 576 TGCGCA Hin1I 817 GACGTC G[GA]^CG[CT]C 1733 GACGCC MluNI 725 TGGCCA TGG^CCA 956 TGGCCA MroI 1013 TCCGGA T^CCGGA 1344 TCCGGA MscI 725 TGGCCA TGG^CCA 956 TGGCCA MseI 1280 TTAA T^TAA 2195 TTAA PleI 1974 GACTC GAGTC(4/5) 844 GAGTC Tru9I 1280 TTAA T^TAA 2195 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I SbfI ScrFI SfaNI SfcI SmlI Sse8387I StyI TaqI TseI Tsp45I TspRI TthHB8I XhoII