(Circular) (Six-base) MAPSORT of: pFN21AB5773.seq Check: 5126 from: 1 to: 7171 pFN21AB5773 7171 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2826 4981 278 Size: 53 83 186 936 1290 2155 2468 Fragments arranged by size: 2468 2155 1290 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2080 4514 1937 Size: 143 2434 4594 Fragments arranged by size: 4594 2434 143 Acc65I G'GTAC_C Cuts at: 3384 4492 3384 Size: 1108 6063 AclI AA'CG_TT Cuts at: 1563 5298 5671 1563 Size: 3735 373 3063 Fragments arranged by size: 3735 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2438 2693 3857 4115 4249 4408 5234 6618 Size: 806 255 1164 258 134 159 826 1384 Cuts at: 6618 6886 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1164 826 806 268 258 255 159 134 AfeI AGC'GCT Cuts at: 3114 3114 Size: 7171 AflII C'TTAA_G Cuts at: 820 1017 3284 820 Size: 197 2267 4707 Fragments arranged by size: 4707 2267 197 AflIII A'CryG_T Cuts at: 1203 2154 4230 6070 7047 1203 Size: 951 2076 1840 977 1327 Fragments arranged by size: 2076 1840 1327 977 951 AgeI A'CCGG_T Cuts at: 6002 6002 Size: 7171 AhdI GACnn_n'nnGTC Cuts at: 5900 5900 Size: 7171 AleI CACnn'nnGTG Cuts at: 1166 3761 1166 Size: 2595 4576 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2729 3593 1184 Size: 1545 864 4762 Fragments arranged by size: 4762 1545 864 AlwNI CAG_nnn'CTG Cuts at: 2371 2614 2647 2759 3064 3103 4010 4444 4914 Size: 243 33 112 305 39 907 434 470 Cuts at: 4914 6486 2371 Size: 1572 3056 Fragments arranged by size: 3056 1572 907 470 434 305 243 112 39 33 ApaI G_GGCC'C Cuts at: 2298 3182 3964 2298 Size: 884 782 5505 Fragments arranged by size: 5505 884 782 ApaLI G'TGCA_C Cuts at: 3428 5228 6384 3428 Size: 1800 1156 4215 Fragments arranged by size: 4215 1800 1156 ApoI r'AATT_y Cuts at: 1493 4480 4706 4858 6967 1493 Size: 2987 226 152 2109 1697 Fragments arranged by size: 2987 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5725 160 Size: 872 4693 1606 Fragments arranged by size: 4693 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7171 AvaI C'yCGr_G Cuts at: 1870 1943 3045 3400 4119 4496 1870 Size: 73 1102 355 719 377 4545 Fragments arranged by size: 4545 1102 719 377 355 73 BamHI G'GATC_C Cuts at: 3831 4501 4868 3831 Size: 670 367 6134 Fragments arranged by size: 6134 670 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3384 4492 5947 618 Size: 325 217 627 1597 1108 1455 1842 Fragments arranged by size: 1842 1597 1455 1108 627 325 217 BanII G_rGCy'C Cuts at: 1391 2298 2385 2437 2892 3018 3182 3192 3964 Size: 907 87 52 455 126 164 10 772 Cuts at: 3964 4490 1391 Size: 526 4072 Fragments arranged by size: 4072 907 772 526 455 164 126 87 52 10 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3983 928 Size: 971 2084 4116 Fragments arranged by size: 4116 2084 971 BbvCI CC'TCA_GC Cuts at: 4295 4295 Size: 7171 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2321 2355 5362 5396 1903 Size: 34 384 34 3007 34 3678 Fragments arranged by size: 3678 3007 384 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5063 6273 5063 Size: 1210 5961 BclI T'GATC_A Cuts at: 1548 1548 Size: 7171 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2037 5782 136 Size: 107 122 71 631 605 365 3745 1525 Fragments arranged by size: 3745 1525 631 605 365 122 107 71 BglII A'GATC_T Cuts at: 7166 7166 Size: 7171 BlpI GC'TnA_GC Cuts at: 2309 3417 4588 2309 Size: 1108 1171 4892 Fragments arranged by size: 4892 1171 1108 Bme1580I G_kGCm'C Cuts at: 1790 2298 2455 3182 3432 3654 3964 5232 6388 Size: 508 157 727 250 222 310 1268 1156 Cuts at: 6388 1790 Size: 2573 Fragments arranged by size: 2573 1268 1156 727 508 310 250 222 157 BmgBI CAC'GTC Cuts at: 1318 3231 4231 1318 Size: 1913 1000 4258 Fragments arranged by size: 4258 1913 1000 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4902 5860 442 Size: 416 53 83 399 24 3485 958 1753 Fragments arranged by size: 3485 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7171 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3806 3806 Size: 7171 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2105 2717 2876 2897 3188 3296 4046 5831 Size: 866 612 159 21 291 108 750 1785 Cuts at: 5831 1239 Size: 2579 Fragments arranged by size: 2579 1785 866 750 612 291 159 108 21 Bpu10I CC'TnA_GC Cuts at: 2252 2300 4295 2252 Size: 48 1995 5128 Fragments arranged by size: 5128 1995 48 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2879 3788 5296 6161 6459 2879 Size: 909 1508 865 298 3591 Fragments arranged by size: 3591 1508 909 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4253 5834 882 Size: 617 2754 1581 2219 Fragments arranged by size: 2754 2219 1581 617 BsaAI yAC'GTr Cuts at: 493 1204 3478 4962 493 Size: 711 2274 1484 2702 Fragments arranged by size: 2702 2274 1484 711 BsaBI GATnn'nnATC Cuts at: 7165 7165 Size: 7171 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2823 3739 4978 5360 Size: 53 83 186 936 1290 916 1239 382 Cuts at: 5360 5995 275 Size: 635 1451 Fragments arranged by size: 1451 1290 1239 936 916 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3018 3349 5604 6002 6276 6423 6834 3018 Size: 331 2255 398 274 147 411 3355 Fragments arranged by size: 3355 2255 411 398 331 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3000 3030 3209 3239 4019 Size: 30 165 30 1597 30 179 30 780 Cuts at: 4019 4049 1178 Size: 30 4300 Fragments arranged by size: 4300 1597 780 179 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2669 2876 3044 4207 1186 Size: 1483 207 168 1163 4150 Fragments arranged by size: 4150 1483 1163 207 168 BseYI C'CCAG_C Cuts at: 2109 2566 3064 3141 3603 4392 4418 6374 6870 Size: 457 498 77 462 789 26 1956 496 Cuts at: 6870 2109 Size: 2410 Fragments arranged by size: 2410 1956 789 498 496 462 457 77 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2047 2633 2735 2942 3077 3139 3377 Size: 573 311 586 102 207 135 62 238 Cuts at: 3377 3623 3865 3941 1163 Size: 246 242 76 4393 Fragments arranged by size: 4393 586 573 311 246 242 238 207 135 102 76 62 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4210 4621 5382 6410 1540 Size: 275 186 2209 411 761 1028 2301 Fragments arranged by size: 2301 2209 1028 761 411 275 186 BsiHKAI G_wGCw'C Cuts at: 2385 2437 2892 3018 3174 3432 3602 5232 5317 Size: 52 455 126 156 258 170 1630 85 Cuts at: 5317 6388 2385 Size: 1071 3168 Fragments arranged by size: 3168 1630 1071 455 258 170 156 126 85 52 BsmI GAATG_Cn' Cuts at: 3570 4682 4775 3570 Size: 1112 93 5966 Fragments arranged by size: 5966 1112 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3779 3872 3779 Size: 93 7078 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2298 2385 2437 2455 2892 3018 3174 Size: 399 508 87 52 18 437 126 156 Cuts at: 3174 3182 3192 3432 3602 3654 3964 4490 5232 Size: 8 10 240 170 52 310 526 742 Cuts at: 5232 5317 6388 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 526 508 437 399 310 240 170 156 126 87 85 52 52 18 10 8 BspEI T'CCGG_A Cuts at: 3018 3349 6834 3018 Size: 331 3485 3355 Fragments arranged by size: 3485 3355 331 BspHI T'CATG_A Cuts at: 5060 6790 5060 Size: 1730 5441 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2655 3097 4526 844 Size: 1811 442 1429 3489 Fragments arranged by size: 3489 1811 1429 442 BsrBI CCG'CTC Cuts at: 1394 1823 3052 5058 1394 Size: 429 1229 2006 3507 Fragments arranged by size: 3507 2006 1229 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5666 5840 65 Size: 1183 4418 174 1396 Fragments arranged by size: 4418 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5815 6002 1700 Size: 110 4005 187 2869 Fragments arranged by size: 4005 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7171 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7171 BssSI C'ACGA_G Cuts at: 2651 5231 6243 2651 Size: 2580 1012 3579 Fragments arranged by size: 3579 2580 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2059 2371 2647 2759 3082 3103 3151 4038 Size: 1265 312 276 112 323 21 48 887 Cuts at: 4038 794 Size: 3927 Fragments arranged by size: 3927 1265 887 323 312 276 112 48 21 BstBI TT'CG_AA Cuts at: 5975 5975 Size: 7171 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7171 BstXI CCAn_nnnn'nTGG Cuts at: 2971 2971 Size: 7171 BstYI r'GATC_y Cuts at: 1972 2281 2679 3279 3831 4501 4868 5253 5270 Size: 309 398 600 552 670 367 385 17 Cuts at: 5270 6722 6808 6820 7166 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 670 600 552 398 385 367 346 309 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2775 4150 4191 2775 Size: 1375 41 5755 Fragments arranged by size: 5755 1375 41 BtgI C'CryG_G Cuts at: 513 1065 2004 2041 3388 513 Size: 552 939 37 1347 4296 Fragments arranged by size: 4296 1347 939 552 37 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6432 Fragments arranged by size: 6432 683 56 BtsI GCAGTG_nn' Cuts at: 2366 2636 2852 3092 4394 4687 5481 5501 2366 Size: 270 216 240 1302 293 794 20 4036 Fragments arranged by size: 4036 1302 794 293 270 240 216 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7171 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3607 3642 529 Size: 35 3043 35 4058 Fragments arranged by size: 4058 3043 35 35 DraI TTT'AAA Cuts at: 4475 4475 Size: 7171 DraIII CAC_nnn'GTG Cuts at: 2834 2834 Size: 7171 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6178 809 Size: 507 4862 1802 Fragments arranged by size: 4862 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2730 2961 4207 Size: 54 1425 50 275 18 900 231 1246 Cuts at: 4207 4618 5507 7136 8 Size: 411 889 1629 43 Fragments arranged by size: 1629 1425 1246 900 889 411 275 231 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4207 4618 1537 Size: 275 2395 411 4090 Fragments arranged by size: 4090 2395 411 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2020 2414 3021 3617 5101 1353 Size: 667 394 607 596 1484 3423 Fragments arranged by size: 3423 1484 667 607 596 394 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3299 5758 6132 6278 387 Size: 1064 138 1710 2459 374 146 1280 Fragments arranged by size: 2459 1710 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2383 2435 2890 3016 2383 Size: 52 455 126 6538 Fragments arranged by size: 6538 455 126 52 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2105 2438 2693 2717 2876 2897 3188 Size: 393 473 333 255 24 159 21 291 Cuts at: 3188 3296 3857 4046 4115 4249 4408 5234 5831 Size: 108 561 189 69 134 159 826 597 Cuts at: 5831 6618 6886 1239 Size: 787 268 1524 Fragments arranged by size: 1524 826 787 597 561 473 393 333 291 268 255 189 159 159 134 108 69 24 21 EcoNI CCTnn'n_nnAGG Cuts at: 2651 2714 2651 Size: 63 7108 EcoO109I rG'GnC_Cy Cuts at: 2047 2248 2294 2295 2644 3179 3682 3960 4051 Size: 201 46 1 349 535 503 278 91 Cuts at: 4051 4156 4195 4275 2047 Size: 105 39 80 4943 Fragments arranged by size: 4943 535 503 349 278 201 105 91 80 46 39 1 EcoRI G'AATT_C Cuts at: 4480 4858 4480 Size: 378 6793 FspI TGC'GCA Cuts at: 2525 2583 5677 2525 Size: 58 3094 4019 Fragments arranged by size: 4019 3094 58 HaeII r_GCGC'y Cuts at: 1678 3116 6318 1678 Size: 1438 3202 2531 Fragments arranged by size: 3202 2531 1438 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2273 2305 3209 3241 5812 Size: 32 135 32 701 32 904 32 2571 Cuts at: 5812 5844 5886 5918 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2571 904 701 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4515 4761 669 Size: 988 281 2577 246 3079 Fragments arranged by size: 3079 2577 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7171 HpaI GTT'AAC Cuts at: 4761 4761 Size: 7171 KpnI G_GTAC'C Cuts at: 3388 4496 3388 Size: 1108 6063 MfeI C'AATT_G Cuts at: 4770 4770 Size: 7171 MluI A'CGCG_T Cuts at: 7047 7047 Size: 7171 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6285 6469 1305 Size: 4980 184 2007 Fragments arranged by size: 4980 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2732 2963 10 Size: 54 1425 343 900 231 4218 Fragments arranged by size: 4218 1425 900 343 231 54 MslI CAynn'nnrTG Cuts at: 518 1166 2969 3761 3908 5129 5488 5647 518 Size: 648 1803 792 147 1221 359 159 2042 Fragments arranged by size: 2042 1803 1221 792 648 359 159 147 MspA1I CmG'CkG Cuts at: 1606 2113 2371 2756 2875 2896 3004 3061 3337 Size: 507 258 385 119 21 108 57 276 Cuts at: 3337 3379 3735 4007 4414 4587 5264 6412 6657 Size: 42 356 272 407 173 677 1148 245 Cuts at: 6657 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 507 407 385 356 276 272 258 245 173 119 108 57 42 21 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5680 Fragments arranged by size: 5680 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7171 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7171 NotI GC'GGCC_GC Cuts at: 4618 4618 Size: 7171 NspI r_CATG'y Cuts at: 1132 1229 2158 4529 6074 1132 Size: 97 929 2371 1545 2229 Fragments arranged by size: 2371 2229 1545 929 97 PasI CC'CwG_GG Cuts at: 1348 2035 1348 Size: 687 6484 PciI A'CATG_T Cuts at: 2154 6070 2154 Size: 3916 3255 PflMI CCAn_nnn'nTGG Cuts at: 1108 3529 1108 Size: 2421 4750 PfoI T'CCnGG_A Cuts at: 2322 2322 Size: 7171 PmeI GTTT'AAAC Cuts at: 4475 4475 Size: 7171 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2960 2992 5240 5272 6753 6785 2960 Size: 32 2248 32 1481 32 3346 Fragments arranged by size: 3346 2248 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2047 2644 3682 4051 2047 Size: 597 1038 369 5167 Fragments arranged by size: 5167 1038 597 369 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7171 PsiI TTA'TAA Cuts at: 4741 4741 Size: 7171 PspOMI G'GGCC_C Cuts at: 2294 3178 3960 2294 Size: 884 782 5505 Fragments arranged by size: 5505 884 782 PstI C_TGCA'G Cuts at: 830 2055 2166 2376 2643 2688 2755 3099 3108 Size: 1225 111 210 267 45 67 344 9 Cuts at: 3108 3138 3159 3213 3342 3990 4449 4523 830 Size: 30 21 54 129 648 459 74 3478 Fragments arranged by size: 3478 1225 648 459 344 267 210 129 111 74 67 54 45 30 21 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7171 PvuII CAG'CTG Cuts at: 2371 2756 2896 3004 3061 3379 4007 2371 Size: 385 140 108 57 318 628 5535 Fragments arranged by size: 5535 628 385 318 140 108 57 SacI G_AGCT'C Cuts at: 2385 2437 2892 3018 2385 Size: 52 455 126 6538 Fragments arranged by size: 6538 455 126 52 SalI G'TCGA_C Cuts at: 1936 4513 1936 Size: 2577 4594 SapI GCTCTTCn'nnn_ Cuts at: 2414 2414 Size: 7171 SbfI CC_TGCA'GG Cuts at: 2166 2688 3213 4523 2166 Size: 522 525 1310 4814 Fragments arranged by size: 4814 1310 525 522 ScaI AGT'ACT Cuts at: 5419 7131 5419 Size: 1712 5459 SexAI A'CCwGG_T Cuts at: 3684 3684 Size: 7171 SfcI C'TryA_G Cuts at: 826 1045 2051 2162 2350 2372 2639 2684 2751 Size: 219 1006 111 188 22 267 45 67 Cuts at: 2751 3095 3104 3134 3155 3209 3338 3818 3986 Size: 344 9 30 21 54 129 480 168 Cuts at: 3986 4445 4519 5654 6335 6526 826 Size: 459 74 1135 681 191 1471 Fragments arranged by size: 1471 1135 1006 681 480 459 344 267 219 191 188 168 129 111 74 67 54 45 30 22 21 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7171 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7171 SmaI CCC'GGG Cuts at: 1872 3047 4498 1872 Size: 1175 1451 4545 Fragments arranged by size: 4545 1451 1175 SmlI C'TyrA_G Cuts at: 820 1017 1943 2858 3284 3803 5275 6176 6438 Size: 197 926 915 426 519 1472 901 262 Cuts at: 6438 820 Size: 1553 Fragments arranged by size: 1553 1472 926 915 901 519 426 262 197 SnaBI TAC'GTA Cuts at: 493 4962 493 Size: 4469 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7171 SphI G_CATG'C Cuts at: 1132 4529 1132 Size: 3397 3774 SspI AAT'ATT Cuts at: 5 52 5095 5 Size: 47 5043 2081 Fragments arranged by size: 5043 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7171 StyI C'CwwG_G Cuts at: 513 1065 2004 2508 2788 3528 3654 4610 513 Size: 552 939 504 280 740 126 956 3074 Fragments arranged by size: 3074 956 939 740 552 504 280 126 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3053 3283 3617 3655 3683 3899 5198 Size: 1255 1473 230 334 38 28 216 1299 Cuts at: 5198 5215 5368 5553 5998 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1473 1299 1255 445 334 230 216 185 153 38 28 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3843 5417 7129 Size: 274 80 33 51 1443 1866 1574 1712 Cuts at: 7129 96 Size: 138 Fragments arranged by size: 1866 1712 1574 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2141 2173 2808 2840 4019 4051 7131 7163 2141 Size: 32 635 32 1179 32 3080 32 2149 Fragments arranged by size: 3080 2149 1179 635 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7171 XbaI T'CTAG_A Cuts at: 4507 4507 Size: 7171 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7171 XmaI C'CCGG_G Cuts at: 1870 3045 4496 1870 Size: 1175 1451 4545 Fragments arranged by size: 4545 1451 1175 XmnI GAAnn'nnTTC Cuts at: 3561 5300 3561 Size: 1739 5432 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2824 4979 276 Size: 53 83 186 936 1290 2155 2468 Fragments arranged by size: 2468 2155 1290 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI