(Linear) (Six-base) MAPSORT of: KIBB5773.seq Check: 2203 from: 1 to: 2466 KIBB5773.orf With 182 enzymes: SgfI * July 15, 2010 18:29 .. AatII G_ACGT'C Cuts at: 0 821 2466 Size: 821 1645 AccI GT'mk_AC Cuts at: 0 75 2466 Size: 75 2391 Acc65I G'GTAC_C Cuts at: 0 1379 2466 Size: 1379 1087 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 433 688 1852 2110 2244 2403 2466 Size: 433 255 1164 258 134 159 63 Fragments arranged by size: 1164 433 258 255 159 134 63 AfeI AGC'GCT Cuts at: 0 1109 2466 Size: 1109 1357 AflII C'TTAA_G Cuts at: 0 1279 2466 Size: 1279 1187 AflIII A'CryG_T Cuts at: 0 149 2225 2466 Size: 149 2076 241 Fragments arranged by size: 2076 241 149 AleI CACnn'nnGTG Cuts at: 0 1756 2466 Size: 1756 710 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 724 1588 2466 Size: 724 864 878 Fragments arranged by size: 878 864 724 AlwNI CAG_nnn'CTG Cuts at: 0 366 609 642 754 1059 1098 2005 2439 Size: 366 243 33 112 305 39 907 434 Cuts at: 2439 2466 Size: 27 Fragments arranged by size: 907 434 366 305 243 112 39 33 27 ApaI G_GGCC'C Cuts at: 0 293 1177 1959 2466 Size: 293 884 782 507 Fragments arranged by size: 884 782 507 293 ApaLI G'TGCA_C Cuts at: 0 1423 2466 Size: 1423 1043 AvaI C'yCGr_G Cuts at: 0 1040 1395 2114 2466 Size: 1040 355 719 352 Fragments arranged by size: 1040 719 355 352 BamHI G'GATC_C Cuts at: 0 1826 2466 Size: 1826 640 BanI G'GyrC_C Cuts at: 0 1379 2466 Size: 1379 1087 BanII G_rGCy'C Cuts at: 0 293 380 432 887 1013 1177 1187 1959 Size: 293 87 52 455 126 164 10 772 Cuts at: 1959 2466 Size: 507 Fragments arranged by size: 772 507 455 293 164 126 87 52 10 BbsI GAAGACnn'nnnn_ Cuts at: 0 1978 2466 Size: 1978 488 BbvCI CC'TCA_GC Cuts at: 0 2290 2466 Size: 2290 176 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 316 350 2466 Size: 316 34 2116 Fragments arranged by size: 2116 316 34 BglI GCCn_nnn'nGGC Cuts at: 0 32 2466 Size: 32 2434 BlpI GC'TnA_GC Cuts at: 0 304 1412 2466 Size: 304 1108 1054 Fragments arranged by size: 1108 1054 304 Bme1580I G_kGCm'C Cuts at: 0 293 450 1177 1427 1649 1959 2466 Size: 293 157 727 250 222 310 507 Fragments arranged by size: 727 507 310 293 250 222 157 BmgBI CAC'GTC Cuts at: 0 1226 2226 2466 Size: 1226 1000 240 Fragments arranged by size: 1226 1000 240 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1801 2466 Size: 1801 665 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 100 712 871 892 1183 1291 2041 2466 Size: 100 612 159 21 291 108 750 425 Fragments arranged by size: 750 612 425 291 159 108 100 21 Bpu10I CC'TnA_GC Cuts at: 0 247 295 2290 2466 Size: 247 48 1995 176 Fragments arranged by size: 1995 247 176 48 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 874 1783 2466 Size: 874 909 683 Fragments arranged by size: 909 874 683 BsaI GGTCTCn'nnnn_ Cuts at: 0 2248 2466 Size: 2248 218 BsaAI yAC'GTr Cuts at: 0 1473 2466 Size: 1473 993 BsaHI Gr'CG_yC Cuts at: 0 818 1734 2466 Size: 818 916 732 Fragments arranged by size: 916 818 732 BsaWI w'CCGG_w Cuts at: 0 1013 1344 2466 Size: 1013 331 1122 Fragments arranged by size: 1122 1013 331 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 995 1025 1204 1234 2014 2044 2466 Size: 995 30 179 30 780 30 422 Fragments arranged by size: 995 780 422 179 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 664 871 1039 2202 2466 Size: 664 207 168 1163 264 Fragments arranged by size: 1163 664 264 207 168 BseYI C'CCAG_C Cuts at: 0 104 561 1059 1136 1598 2387 2413 2466 Size: 104 457 498 77 462 789 26 53 Fragments arranged by size: 789 498 462 457 104 77 53 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 42 628 730 937 1072 1134 1372 1618 Size: 42 586 102 207 135 62 238 246 Cuts at: 1618 1860 1936 2466 Size: 242 76 530 Fragments arranged by size: 586 530 246 242 238 207 135 102 76 62 42 BsiEI CG_ry'CG Cuts at: 0 2205 2466 Size: 2205 261 BsiHKAI G_wGCw'C Cuts at: 0 380 432 887 1013 1169 1427 1597 2466 Size: 380 52 455 126 156 258 170 869 Fragments arranged by size: 869 455 380 258 170 156 126 52 BsmI GAATG_Cn' Cuts at: 0 1565 2466 Size: 1565 901 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1774 1867 2466 Size: 1774 93 599 Fragments arranged by size: 1774 599 93 Bsp1286I G_dGCh'C Cuts at: 0 293 380 432 450 887 1013 1169 1177 Size: 293 87 52 18 437 126 156 8 Cuts at: 1177 1187 1427 1597 1649 1959 2466 Size: 10 240 170 52 310 507 Fragments arranged by size: 507 437 310 293 240 170 156 126 87 52 52 18 10 8 BspEI T'CCGG_A Cuts at: 0 1013 1344 2466 Size: 1013 331 1122 Fragments arranged by size: 1122 1013 331 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 650 1092 2466 Size: 650 442 1374 Fragments arranged by size: 1374 650 442 BsrBI CCG'CTC Cuts at: 0 1047 2466 Size: 1047 1419 BssSI C'ACGA_G Cuts at: 0 646 2466 Size: 646 1820 BstAPI GCAn_nnn'nTGC Cuts at: 0 54 366 642 754 1077 1098 1146 2033 Size: 54 312 276 112 323 21 48 887 Cuts at: 2033 2466 Size: 433 Fragments arranged by size: 887 433 323 312 276 112 54 48 21 BstXI CCAn_nnnn'nTGG Cuts at: 0 966 2466 Size: 966 1500 BstYI r'GATC_y Cuts at: 0 276 674 1274 1826 2466 Size: 276 398 600 552 640 Fragments arranged by size: 640 600 552 398 276 Bsu36I CC'TnA_GG Cuts at: 0 770 2145 2186 2466 Size: 770 1375 41 280 Fragments arranged by size: 1375 770 280 41 BtgI C'CryG_G Cuts at: 0 36 1383 2466 Size: 36 1347 1083 Fragments arranged by size: 1347 1083 36 BtsI GCAGTG_nn' Cuts at: 0 361 631 847 1087 2389 2466 Size: 361 270 216 240 1302 77 Fragments arranged by size: 1302 361 270 240 216 77 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1602 1637 2466 Size: 1602 35 829 Fragments arranged by size: 1602 829 35 DraIII CAC_nnn'GTG Cuts at: 0 829 2466 Size: 829 1637 EaeI y'GGCC_r Cuts at: 0 725 956 2202 2466 Size: 725 231 1246 264 Fragments arranged by size: 1246 725 264 231 EagI C'GGCC_G Cuts at: 0 2202 2466 Size: 2202 264 EarI CTCTTCn'nnn_ Cuts at: 0 15 409 1016 1612 2466 Size: 15 394 607 596 854 Fragments arranged by size: 854 607 596 394 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1294 2466 Size: 1294 1172 EcoICRI GAG'CTC Cuts at: 0 378 430 885 1011 2466 Size: 378 52 455 126 1455 Fragments arranged by size: 1455 455 378 126 52 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 100 433 688 712 871 892 1183 1291 Size: 100 333 255 24 159 21 291 108 Cuts at: 1291 1852 2041 2110 2244 2403 2466 Size: 561 189 69 134 159 63 Fragments arranged by size: 561 333 291 255 189 159 159 134 108 100 69 63 24 21 EcoNI CCTnn'n_nnAGG Cuts at: 0 646 709 2466 Size: 646 63 1757 Fragments arranged by size: 1757 646 63 EcoO109I rG'GnC_Cy Cuts at: 0 42 243 289 290 639 1174 1677 1955 Size: 42 201 46 1 349 535 503 278 Cuts at: 1955 2046 2151 2190 2270 2466 Size: 91 105 39 80 196 Fragments arranged by size: 535 503 349 278 201 196 105 91 80 46 42 39 1 FspI TGC'GCA Cuts at: 0 520 578 2466 Size: 520 58 1888 Fragments arranged by size: 1888 520 58 HaeII r_GCGC'y Cuts at: 0 1111 2466 Size: 1111 1355 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 268 300 1204 1236 2466 Size: 268 32 904 32 1230 Fragments arranged by size: 1230 904 268 32 32 KpnI G_GTAC'C Cuts at: 0 1383 2466 Size: 1383 1083 MscI TGG'CCA Cuts at: 0 727 958 2466 Size: 727 231 1508 Fragments arranged by size: 1508 727 231 MslI CAynn'nnrTG Cuts at: 0 964 1756 1903 2466 Size: 964 792 147 563 Fragments arranged by size: 964 792 563 147 MspA1I CmG'CkG Cuts at: 0 108 366 751 870 891 999 1056 1332 Size: 108 258 385 119 21 108 57 276 Cuts at: 1332 1374 1730 2002 2409 2466 Size: 42 356 272 407 57 Fragments arranged by size: 407 385 356 276 272 258 119 108 108 57 57 42 21 NspI r_CATG'y Cuts at: 0 153 2466 Size: 153 2313 PasI CC'CwG_GG Cuts at: 0 30 2466 Size: 30 2436 PciI A'CATG_T Cuts at: 0 149 2466 Size: 149 2317 PflMI CCAn_nnn'nTGG Cuts at: 0 1524 2466 Size: 1524 942 PfoI T'CCnGG_A Cuts at: 0 317 2466 Size: 317 2149 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 955 987 2466 Size: 955 32 1479 Fragments arranged by size: 1479 955 32 PpuMI rG'GwC_Cy Cuts at: 0 42 639 1677 2046 2466 Size: 42 597 1038 369 420 Fragments arranged by size: 1038 597 420 369 42 PspOMI G'GGCC_C Cuts at: 0 289 1173 1955 2466 Size: 289 884 782 511 Fragments arranged by size: 884 782 511 289 PstI C_TGCA'G Cuts at: 0 50 161 371 638 683 750 1094 1103 Size: 50 111 210 267 45 67 344 9 Cuts at: 1103 1133 1154 1208 1337 1985 2444 2466 Size: 30 21 54 129 648 459 22 Fragments arranged by size: 648 459 344 267 210 129 111 67 54 50 45 30 22 21 9 PvuII CAG'CTG Cuts at: 0 366 751 891 999 1056 1374 2002 2466 Size: 366 385 140 108 57 318 628 464 Fragments arranged by size: 628 464 385 366 318 140 108 57 SacI G_AGCT'C Cuts at: 0 380 432 887 1013 2466 Size: 380 52 455 126 1453 Fragments arranged by size: 1453 455 380 126 52 SapI GCTCTTCn'nnn_ Cuts at: 0 409 2466 Size: 409 2057 SbfI CC_TGCA'GG Cuts at: 0 161 683 1208 2466 Size: 161 522 525 1258 Fragments arranged by size: 1258 525 522 161 SexAI A'CCwGG_T Cuts at: 0 1679 2466 Size: 1679 787 SfcI C'TryA_G Cuts at: 0 46 157 345 367 634 679 746 1090 Size: 46 111 188 22 267 45 67 344 Cuts at: 1090 1099 1129 1150 1204 1333 1813 1981 2440 Size: 9 30 21 54 129 480 168 459 Cuts at: 2440 2466 Size: 26 Fragments arranged by size: 480 459 344 267 188 168 129 111 67 54 46 45 30 26 22 21 9 SmaI CCC'GGG Cuts at: 0 1042 2466 Size: 1042 1424 SmlI C'TyrA_G Cuts at: 0 853 1279 1798 2466 Size: 853 426 519 668 Fragments arranged by size: 853 668 519 426 StyI C'CwwG_G Cuts at: 0 503 783 1523 1649 2466 Size: 503 280 740 126 817 Fragments arranged by size: 817 740 503 280 126 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1048 1278 1612 1650 1678 1894 2466 Size: 1048 230 334 38 28 216 572 Fragments arranged by size: 1048 572 334 230 216 38 28 TatI w'GTAC_w Cuts at: 0 1838 2466 Size: 1838 628 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 136 168 803 835 2014 2046 2466 Size: 136 32 635 32 1179 32 420 Fragments arranged by size: 1179 635 420 136 32 32 32 XmaI C'CCGG_G Cuts at: 0 1040 2466 Size: 1040 1426 XmnI GAAnn'nnTTC Cuts at: 0 1556 2466 Size: 1556 910 ZraI GAC'GTC Cuts at: 0 819 2466 Size: 819 1647 Enzymes that do cut: AatII AccI Acc65I AcuI AfeI AflII AflIII AleI AlfI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI CspCI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KpnI MscI MslI MspA1I NspI PasI PciI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SacI SapI SbfI SexAI SfcI SmaI SmlI StyI TaqII TatI TstI XmaI XmnI ZraI Enzymes that do not cut: AarI AclI AgeI AhdI AloI ApoI AscI AseI AsiSI AvrII BaeI BbeI BciVI BclI BfrBI BglII BmrI BmtI BsaBI BsiWI BspHI BsrDI BsrFI BsrGI BssHII BstBI BstEII BstZ17I BtgZI ClaI DraI DrdI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KasI MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI