Restriction map for
pFN21AA0928
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# NO CUT ENZYMES ( 68 enzymes) #
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AatII Acc65I AccIII AcyI AflIII
AgeI Alw44I ApaI ApaLI AscI
Asp718I AvrII BbeI BbrPI BglI
BlnI BsaAI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BspEI BspLU11I
BsrFI BssHII BstAPI Cfr10I CpoI
EagI EclXI Eco52I FseI Hin1I
HindIII HpaI KasI KpnI MluI
MroI NaeI NarI NgoMIV NheI
NotI NruI PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PvuI RsrII SalI SbfI SfiI
SfoI SgrAI SnaBI SpeI Sse8387I
SwaI Van91I XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 5171 CGCG CG^CG
AclI 3462 AACGTT AA^CGTT
AflII 688 CTTAAG C^TTAAG
AseI 3523 ATTAAT AT^TAAT
AsnI 3523 ATTAAT AT^TAAT
AspI 3903 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4011 TGCGCA TGC^GCA
BalI 4877 TGGCCA TGG^CCA
BamHI 4536 GGATCC G^GATCC
BbvCI 24 CCTCAGC CCTCAGC(-5/-2)
BcgI 2233 CGAAGGCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1692 GGATAC GTATCC(6/5)
BfrI 688 CTTAAG C^TTAAG
BsaBI 2275 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 2723 GCATTC GAATGC(1/-1)
BsrBI 3937 GAGCGG CCGCTC(-3/-3)
BsrDI 4522 GCAATG GCAATG(2/0)
BssSI 1868 CACGAG CACGAG(-5/-1)
BstBI 894 TTCGAA TT^CGAA
BstEII 2472 GGTTACC G^GT[ACGT]ACC
BstPI 2472 GGTTACC G^GT[ACGT]ACC
BstUI 5171 CGCG CG^CG
DraIII 1070 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 5046 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2472 GGTTACC G^GT[ACGT]ACC
EcoRV 2804 GATATC GAT^ATC
EcoT22I 389 ATGCAT ATGCA^T
FauI 5169 CCCGC CCCGC(4/6)
FspI 4011 TGCGCA TGC^GCA
HgaI 2611 GACGC GACGC(5/10)
KspI 5170 CCGCGG CCGC^GG
MamI 2275 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3377 CAATTG C^AATTG
MluNI 4877 TGGCCA TGG^CCA
MscI 4877 TGGCCA TGG^CCA
MunI 3377 CAATTG C^AATTG
MvnI 5171 CGCG CG^CG
NsiI 389 ATGCAT ATGCA^T
NspV 894 TTCGAA TT^CGAA
PflFI 3903 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3523 ATTAAT AT^TAAT
Psp1406I 3462 AACGTT AA^CGTT
SacI 5290 GAGCTC GAGCT^C
SacII 5170 CCGCGG CCGC^GG
SfuI 894 TTCGAA TT^CGAA
SmaI 5184 CCCGGG CCC^GGG
Tth111I 3903 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3426 TCTAGA T^CTAGA
XmaCI 5184 CCCGGG C^CCGGG
XmaI 5184 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccI 735 GTATAC GT^[AC][GT]AC
2739 GTATAC
AhdI 3439 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5200 GACCTGCGGTC
Aor51HI 3030 AGCGCT AGC^GCT
5105 AGCGCT
AspEI 3439 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5200 GACCTGCGGTC
BanI 1662 GGCACC G^G[CT][GA]CC
5449 GGTGCC
BlpI 3468 GCTCAGC GC^T[ACGT]AGC
4735 GCTCAGC
Bpu1102I 3468 GCTCAGC GC^T[ACGT]AGC
4735 GCTCAGC
BsaI 1534 GAGACC GGTCTC(1/5)
2197 GAGACC
BspDI 1628 ATCGAT AT^CGAT
2828 ATCGAT
Bst1107I 735 GTATAC GTA^TAC
2739 GTATAC
BstZ17I 735 GTATAC GTA^TAC
2739 GTATAC
CelII 3468 GCTCAGC GC^T[ACGT]AGC
4735 GCTCAGC
ClaI 1628 ATCGAT AT^CGAT
2828 ATCGAT
DrdI 715 GACCTGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5623 GACGGGAAGGTC
Eam1105I 3439 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5200 GACCTGCGGTC
Eco47III 3030 AGCGCT AGC^GCT
5105 AGCGCT
EcoRI 1489 GAATTC G^AATTC
5113 GAATTC
HaeII 3030 AGCGCT [GA]GCGC^[CT]
5105 AGCGCT
NcoI 91 CCATGG C^CATGG
5420 CCATGG
ScaI 222 AGTACT AGT^ACT
5267 AGTACT
SphI 3972 GCATGC GCATG^C
4217 GCATGC
SspI 2909 AATATT AAT^ATT
4116 AATATT
StuI 36 AGGCCT AGG^CCT
1849 AGGCCT
XcmI 3130 CCAGCATCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4097 CCAGCCGCATGGTGG
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# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI Asp700I AspHI
AvaI AvaII BanII BbsI BbvI
BclI BcnI BfaI BglII BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspHI BspMI
BsrGI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RcaI
RsaI SapI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI