(Circular) (Six-base) MAPSORT of: pFN21AA0928.seq Check: 3828 from: 1 to: 10471 pFN21AA0928 10471 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3106 7489 3106 Size: 4383 6088 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8281 278 Size: 53 83 186 936 6745 2468 Fragments arranged by size: 6745 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2741 4745 7814 1937 Size: 804 2004 3069 4594 Fragments arranged by size: 4594 3069 2004 804 Acc65I G'GTAC_C Cuts at: 7792 7792 Size: 10471 AclI AA'CG_TT Cuts at: 1563 5468 8598 8971 1563 Size: 3905 3130 373 3063 Fragments arranged by size: 3905 3130 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2254 4410 4734 5331 5882 6788 8534 9918 Size: 622 2156 324 597 551 906 1746 1384 Cuts at: 9918 10186 1632 Size: 268 1917 Fragments arranged by size: 2156 1917 1746 1384 906 622 597 551 324 268 AfeI AGC'GCT Cuts at: 5037 7112 5037 Size: 2075 8396 AflII C'TTAA_G Cuts at: 820 1017 2693 820 Size: 197 1676 8598 Fragments arranged by size: 8598 1676 197 AflIII A'CryG_T Cuts at: 1203 9370 10347 1203 Size: 8167 977 1327 Fragments arranged by size: 8167 1327 977 AgeI A'CCGG_T Cuts at: 9302 9302 Size: 10471 AhdI GACnn_n'nnGTC Cuts at: 5449 7210 9200 5449 Size: 1761 1990 6720 Fragments arranged by size: 6720 1990 1761 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 10471 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5486 6681 1184 Size: 4302 1195 4974 Fragments arranged by size: 4974 4302 1195 AlwNI CAG_nnn'CTG Cuts at: 4619 4963 5101 6063 7595 8214 9786 4619 Size: 344 138 962 1532 619 1572 5304 Fragments arranged by size: 5304 1572 1532 962 619 344 138 ApaLI G'TGCA_C Cuts at: 8528 9684 8528 Size: 1156 9315 ApoI r'AATT_y Cuts at: 1493 2688 3032 3116 3301 3494 3524 4601 4688 Size: 1195 344 84 185 193 30 1077 87 Cuts at: 4688 4880 5665 6829 7006 7118 7601 7670 7780 Size: 192 785 1164 177 112 483 69 110 Cuts at: 7780 8006 8158 10267 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1195 1164 1077 785 483 344 226 193 192 185 177 152 112 110 87 84 69 30 AseI AT'TA_AT Cuts at: 160 1032 5529 9025 160 Size: 872 4497 3496 1606 Fragments arranged by size: 4497 3496 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10471 AvaI C'yCGr_G Cuts at: 1870 1943 3969 6822 7189 7796 1870 Size: 73 2026 2853 367 607 4545 Fragments arranged by size: 4545 2853 2026 607 367 73 BamHI G'GATC_C Cuts at: 6541 7801 8168 6541 Size: 1260 367 8844 Fragments arranged by size: 8844 1260 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3667 7454 7792 9247 618 Size: 325 217 627 1880 3787 338 1455 1842 Fragments arranged by size: 3787 1880 1842 1455 627 338 325 217 BanII G_rGCy'C Cuts at: 1391 5221 5846 6298 7299 7729 7790 1391 Size: 3830 625 452 1001 430 61 4072 Fragments arranged by size: 4072 3830 1001 625 452 430 61 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2219 3732 4225 7176 928 Size: 971 320 1513 493 2951 4223 Fragments arranged by size: 4223 2951 1513 971 493 320 BbvCI CC'TCA_GC Cuts at: 2030 2030 Size: 10471 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4227 4261 8662 8696 1903 Size: 34 2290 34 4401 34 3678 Fragments arranged by size: 4401 3678 2290 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3691 8363 9573 3691 Size: 4672 1210 4589 Fragments arranged by size: 4672 4589 1210 BclI T'GATC_A Cuts at: 1548 4297 4822 6340 6955 1548 Size: 2749 525 1518 615 5064 Fragments arranged by size: 5064 2749 1518 615 525 BfrBI ATG'CAT Cuts at: 2396 2396 Size: 10471 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 9082 136 Size: 107 122 71 631 605 7410 1525 Fragments arranged by size: 7410 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2356 3756 5515 7376 10466 2356 Size: 1400 1759 1861 3090 2361 Fragments arranged by size: 3090 2361 1861 1759 1400 BlpI GC'TnA_GC Cuts at: 5474 6741 7888 5474 Size: 1267 1147 8057 Fragments arranged by size: 8057 1267 1147 Bme1580I G_kGCm'C Cuts at: 1790 3670 6818 8532 9688 1790 Size: 1880 3148 1714 1156 2573 Fragments arranged by size: 3148 2573 1880 1714 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 10471 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4728 6770 8202 Size: 416 53 83 399 24 3311 2042 1432 Cuts at: 8202 9160 442 Size: 958 1753 Fragments arranged by size: 3311 2042 1753 1432 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10471 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3109 6756 3109 Size: 3647 6824 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4491 4648 5960 6353 7040 7505 9131 1239 Size: 3252 157 1312 393 687 465 1626 2579 Fragments arranged by size: 3252 2579 1626 1312 687 465 393 157 Bpu10I CC'TnA_GC Cuts at: 2030 2206 7293 7370 2030 Size: 176 5087 77 5131 Fragments arranged by size: 5131 5087 176 77 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2345 3781 4553 5801 5848 7355 8596 9461 9759 Size: 1436 772 1248 47 1507 1241 865 298 Cuts at: 9759 2345 Size: 3057 Fragments arranged by size: 3057 1507 1436 1248 1241 865 772 298 47 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3533 4196 9134 882 Size: 617 2034 663 4938 2219 Fragments arranged by size: 4938 2219 2034 663 617 BsaAI yAC'GTr Cuts at: 493 1204 8262 493 Size: 711 7058 2702 Fragments arranged by size: 7058 2702 711 BsaBI GATnn'nnATC Cuts at: 4284 10465 4284 Size: 6181 4290 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 8278 8660 9295 275 Size: 53 83 186 936 6745 382 635 1451 Fragments arranged by size: 6745 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 8904 9302 9576 9723 10134 8904 Size: 398 274 147 411 9241 Fragments arranged by size: 9241 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 6378 6408 1178 Size: 30 165 30 4975 30 5241 Fragments arranged by size: 5241 4975 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3032 4190 5222 5332 5416 5851 6272 6275 Size: 1846 1158 1032 110 84 435 421 3 Cuts at: 6275 6284 6287 6290 7397 1186 Size: 9 3 3 1107 4260 Fragments arranged by size: 4260 1846 1158 1107 1032 435 421 110 84 9 3 3 3 BseYI C'CCAG_C Cuts at: 5168 5691 5696 6101 6703 9674 10170 5168 Size: 523 5 405 602 2971 496 5469 Fragments arranged by size: 5469 2971 602 523 496 405 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2976 3008 4094 4578 5385 7343 1163 Size: 573 1240 32 1086 484 807 1958 4291 Fragments arranged by size: 4291 1958 1240 1086 807 573 484 32 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7921 8682 9710 1540 Size: 275 186 5920 761 1028 2301 Fragments arranged by size: 5920 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3090 3535 5817 6777 7299 8532 8617 9688 3090 Size: 445 2282 960 522 1233 85 1071 3873 Fragments arranged by size: 3873 2282 1233 1071 960 522 445 85 BsmI GAATG_Cn' Cuts at: 4728 7982 8075 4728 Size: 3254 93 7124 Fragments arranged by size: 7124 3254 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3090 3535 3670 5221 5817 5846 6298 Size: 399 1300 445 135 1551 596 29 452 Cuts at: 6298 6777 6818 7299 7729 7790 8532 8617 9688 Size: 479 41 481 430 61 742 85 1071 Cuts at: 9688 1391 Size: 2174 Fragments arranged by size: 2174 1551 1300 1071 742 596 481 479 452 445 430 399 135 85 61 41 29 BspEI T'CCGG_A Cuts at: 10134 10134 Size: 10471 BspHI T'CATG_A Cuts at: 2052 2116 5440 8360 10090 2052 Size: 64 3324 2920 1730 2433 Fragments arranged by size: 3324 2920 2433 1730 64 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3106 3555 4600 7215 7489 7826 844 Size: 2262 449 1045 2615 274 337 3489 Fragments arranged by size: 3489 2615 2262 1045 449 337 274 BsrBI CCG'CTC Cuts at: 1394 1823 5944 8358 1394 Size: 429 4121 2414 3507 Fragments arranged by size: 4121 3507 2414 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6534 8966 9140 65 Size: 1183 5286 2432 174 1396 Fragments arranged by size: 5286 2432 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 9115 9302 1700 Size: 110 7305 187 2869 Fragments arranged by size: 7305 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2866 4458 4854 96 Size: 2770 1592 396 5713 Fragments arranged by size: 5713 2770 1592 396 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10471 BssSI C'ACGA_G Cuts at: 3873 8531 9543 3873 Size: 4658 1012 4801 Fragments arranged by size: 4801 4658 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 10471 BstBI TT'CG_AA Cuts at: 2900 9275 2900 Size: 6375 4096 BstEII G'GTnAC_C Cuts at: 1184 4477 1184 Size: 3293 7178 BstXI CCAn_nnnn'nTGG Cuts at: 4672 6109 7465 4672 Size: 1437 1356 7678 Fragments arranged by size: 7678 1437 1356 BstYI r'GATC_y Cuts at: 1972 2356 3406 3756 5515 6541 7376 7801 8168 Size: 384 1050 350 1759 1026 835 425 367 Cuts at: 8168 8553 8570 10022 10108 10120 10466 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1759 1452 1050 1026 835 425 385 384 367 350 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2742 4746 2742 Size: 2004 8467 Bsu36I CC'TnA_GG Cuts at: 3059 6469 7755 3059 Size: 3410 1286 5775 Fragments arranged by size: 5775 3410 1286 BtgI C'CryG_G Cuts at: 513 1065 2096 7175 7425 513 Size: 552 1031 5079 250 3559 Fragments arranged by size: 5079 3559 1031 552 250 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5247 5715 7227 487 Size: 683 56 4021 468 1512 3731 Fragments arranged by size: 4021 3731 1512 683 468 56 BtsI GCAGTG_nn' Cuts at: 4250 4961 5056 5188 6665 7987 8781 8801 4250 Size: 711 95 132 1477 1322 794 20 5920 Fragments arranged by size: 5920 1477 1322 794 711 132 95 20 ClaI AT'CG_AT Cuts at: 1554 3634 4834 1554 Size: 2080 1200 7191 Fragments arranged by size: 7191 2080 1200 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10436 DraI TTT'AAA Cuts at: 2634 4606 4687 4771 5973 7675 7775 2634 Size: 1972 81 84 1202 1702 100 5330 Fragments arranged by size: 5330 1972 1702 1202 100 84 81 DraIII CAC_nnn'GTG Cuts at: 3080 3080 Size: 10471 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2726 7634 9478 809 Size: 507 1410 4908 1844 1802 Fragments arranged by size: 4908 1844 1802 1410 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3891 4422 5778 Size: 54 1425 50 275 18 2061 531 1356 Cuts at: 5778 6882 7520 7918 8807 10436 8 Size: 1104 638 398 889 1629 43 Fragments arranged by size: 2061 1629 1425 1356 1104 889 638 531 398 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7918 1537 Size: 275 6106 4090 Fragments arranged by size: 6106 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3078 3504 3567 3652 4032 4167 4194 5054 Size: 1725 426 63 85 380 135 27 860 Cuts at: 5054 5350 6300 6901 7304 7386 7395 7717 8401 Size: 296 950 601 403 82 9 322 684 Cuts at: 8401 1353 Size: 3423 Fragments arranged by size: 3423 1725 950 860 684 601 426 403 380 322 296 135 85 82 63 27 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4387 9058 9432 9578 387 Size: 1064 138 2798 4671 374 146 1280 Fragments arranged by size: 4671 2798 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 7297 7297 Size: 10471 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2254 4410 4491 4648 4734 5331 5882 Size: 393 622 2156 81 157 86 597 551 Cuts at: 5882 5960 6353 6788 7040 7505 8534 9131 9918 Size: 78 393 435 252 465 1029 597 787 Cuts at: 9918 10186 1239 Size: 268 1524 Fragments arranged by size: 2156 1524 1029 787 622 597 597 551 465 435 393 393 268 252 157 86 81 78 EcoNI CCTnn'n_nnAGG Cuts at: 7055 7055 Size: 10471 EcoO109I rG'GnC_Cy Cuts at: 2078 2945 6808 7195 2078 Size: 867 3863 387 5354 Fragments arranged by size: 5354 3863 867 387 EcoRI G'AATT_C Cuts at: 3494 7118 7780 8158 3494 Size: 3624 662 378 5807 Fragments arranged by size: 5807 3624 662 378 EcoRV GAT'ATC Cuts at: 4811 4811 Size: 10471 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2034 2852 3533 3746 4196 2034 Size: 818 681 213 450 8309 Fragments arranged by size: 8309 818 681 450 213 FspI TGC'GCA Cuts at: 6018 8977 6018 Size: 2959 7512 FspAI rTGC'GCAy Cuts at: 6018 6018 Size: 10471 HaeII r_GCGC'y Cuts at: 1678 5039 7114 9618 1678 Size: 3361 2075 2504 2531 Fragments arranged by size: 3361 2531 2504 2075 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3867 3899 4967 4999 5303 Size: 32 135 32 2295 32 1068 32 304 Cuts at: 5303 5335 5435 5467 6500 6532 7196 7228 9112 Size: 32 100 32 1033 32 664 32 1884 Cuts at: 9112 9144 9186 9218 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2295 1884 1068 1033 664 304 135 100 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2305 6616 7225 7815 8061 669 Size: 988 281 367 4311 609 590 246 3079 Fragments arranged by size: 4311 3079 988 609 590 367 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 10471 HpaI GTT'AAC Cuts at: 8061 8061 Size: 10471 KpnI G_GTAC'C Cuts at: 7796 7796 Size: 10471 MfeI C'AATT_G Cuts at: 5382 8070 5382 Size: 2688 7783 MluI A'CGCG_T Cuts at: 10347 10347 Size: 10471 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 9585 9769 1305 Size: 8280 184 2007 Fragments arranged by size: 8280 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 6884 10 Size: 54 1425 343 5052 3597 Fragments arranged by size: 5052 3597 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5143 5409 6113 6442 6943 6963 7164 Size: 648 3977 266 704 329 501 20 201 Cuts at: 7164 7463 8429 8788 8947 518 Size: 299 966 359 159 2042 Fragments arranged by size: 3977 2042 966 704 648 501 359 329 299 266 201 159 20 MspA1I CmG'CkG Cuts at: 1606 2339 5351 6723 7177 7887 8564 9712 9957 Size: 733 3012 1372 454 710 677 1148 245 Cuts at: 9957 1606 Size: 2120 Fragments arranged by size: 3012 2120 1372 1148 733 710 677 454 245 NcoI C'CATG_G Cuts at: 513 1065 2096 7425 513 Size: 552 1031 5329 3559 Fragments arranged by size: 5329 3559 1031 552 NdeI CA'TA_TG Cuts at: 387 3172 3844 6439 387 Size: 2785 672 2595 4419 Fragments arranged by size: 4419 2785 2595 672 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10471 NotI GC'GGCC_GC Cuts at: 7918 7918 Size: 10471 NsiI A_TGCA'T Cuts at: 2398 2398 Size: 10471 NspI r_CATG'y Cuts at: 1132 1229 3074 5981 6226 7073 7829 9374 1132 Size: 97 1845 2907 245 847 756 1545 2229 Fragments arranged by size: 2907 2229 1845 1545 847 756 245 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 10471 PciI A'CATG_T Cuts at: 9370 9370 Size: 10471 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 10471 PfoI T'CCnGG_A Cuts at: 4213 5884 6330 4213 Size: 1671 446 8354 Fragments arranged by size: 8354 1671 446 PmeI GTTT'AAAC Cuts at: 7775 7775 Size: 10471 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2189 2221 5413 5445 8540 8572 10053 10085 2189 Size: 32 3192 32 3095 32 1481 32 2575 Fragments arranged by size: 3192 3095 2575 1481 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2078 2945 7195 2078 Size: 867 4250 5354 Fragments arranged by size: 5354 4250 867 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 10471 PsiI TTA'TAA Cuts at: 4920 8041 4920 Size: 3121 7350 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2922 2954 2922 Size: 32 10439 PstI C_TGCA'G Cuts at: 830 2049 5088 5206 5465 7714 7823 830 Size: 1219 3039 118 259 2249 109 3478 Fragments arranged by size: 3478 3039 2249 1219 259 118 109 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10471 PvuII CAG'CTG Cuts at: 2339 5351 6723 2339 Size: 3012 1372 6087 Fragments arranged by size: 6087 3012 1372 SacI G_AGCT'C Cuts at: 7299 7299 Size: 10471 SacII CC_GC'GG Cuts at: 7178 7178 Size: 10471 SalI G'TCGA_C Cuts at: 1936 7813 1936 Size: 5877 4594 SanDI GG'GwC_CC Cuts at: 2945 2945 Size: 10471 SapI GCTCTTCn'nnn_ Cuts at: 3078 3652 6901 7304 7717 3078 Size: 574 3249 403 413 5832 Fragments arranged by size: 5832 3249 574 413 403 SbfI CC_TGCA'GG Cuts at: 7823 7823 Size: 10471 ScaI AGT'ACT Cuts at: 2229 7274 8719 10431 2229 Size: 5045 1445 1712 2269 Fragments arranged by size: 5045 2269 1712 1445 SexAI A'CCwGG_T Cuts at: 2316 2439 2721 4222 2316 Size: 123 282 1501 8565 Fragments arranged by size: 8565 1501 282 123 SfcI C'TryA_G Cuts at: 826 1045 2045 2990 4611 5084 5202 5461 6511 Size: 219 1000 945 1621 473 118 259 1050 Cuts at: 6511 7027 7710 7819 8954 9635 9826 826 Size: 516 683 109 1135 681 191 1471 Fragments arranged by size: 1621 1471 1135 1050 1000 945 683 681 516 473 259 219 191 118 109 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10471 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10471 SmaI CCC'GGG Cuts at: 1872 7191 7798 1872 Size: 5319 607 4545 Fragments arranged by size: 5319 4545 607 SmlI C'TyrA_G Cuts at: 820 1017 1943 2360 2693 3760 4532 5816 5863 Size: 197 926 417 333 1067 772 1284 47 Cuts at: 5863 7334 8575 9476 9738 820 Size: 1471 1241 901 262 1553 Fragments arranged by size: 1553 1471 1284 1241 1067 926 901 772 417 333 262 197 47 SnaBI TAC'GTA Cuts at: 493 8262 493 Size: 7769 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10471 SphI G_CATG'C Cuts at: 1132 5981 6226 7829 1132 Size: 4849 245 1603 3774 Fragments arranged by size: 4849 3774 1603 245 SspI AAT'ATT Cuts at: 5 52 4916 6123 8395 5 Size: 47 4864 1207 2272 2081 Fragments arranged by size: 4864 2272 2081 1207 47 StuI AGG'CCT Cuts at: 1517 2043 3856 1517 Size: 526 1813 8132 Fragments arranged by size: 8132 1813 526 StyI C'CwwG_G Cuts at: 513 1065 2096 5596 6079 7425 7910 513 Size: 552 1031 3500 483 1346 485 3074 Fragments arranged by size: 3500 3074 1346 1031 552 485 483 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3685 8498 8515 8668 8853 9298 325 Size: 1255 2105 4813 17 153 185 445 1498 Fragments arranged by size: 4813 2105 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2227 2866 4458 Size: 274 80 33 51 1443 250 639 1592 Cuts at: 4458 4736 4854 4929 5708 7272 7503 8717 10429 Size: 278 118 75 779 1564 231 1214 1712 Cuts at: 10429 96 Size: 138 Fragments arranged by size: 1712 1592 1564 1443 1214 779 639 278 274 250 231 138 118 80 75 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5757 5789 6515 6547 10431 10463 5757 Size: 32 726 32 3884 32 5765 Fragments arranged by size: 5765 3884 726 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5911 1316 Size: 4595 5876 XbaI T'CTAG_A Cuts at: 5431 7807 5431 Size: 2376 8095 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5142 6109 5142 Size: 967 9504 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10471 XmaI C'CCGG_G Cuts at: 1870 7189 7796 1870 Size: 5319 607 4545 Fragments arranged by size: 5319 4545 607 XmnI GAAnn'nnTTC Cuts at: 2112 2350 2670 7360 8600 2112 Size: 238 320 4690 1240 3983 Fragments arranged by size: 4690 3983 1240 320 238 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8279 276 Size: 53 83 186 936 6745 2468 Fragments arranged by size: 6745 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI FseI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI RsrII SfiI SfoI SrfI SwaI