Restriction map for
pFN21AE0395
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# NO CUT ENZYMES ( 91 enzymes) #
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AatII AccIII AclI AcyI AflII
AgeI AocI AscI AviII AvrII
BbeI BbrPI BbvCI BciVI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BseAI BsiWI BspDI
BspEI BspHI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HgaI Hin1I HpaI KasI
KspI MamI MfeI MluI MroI
MunI NarI NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 557 GGTACC G^GTACC
AccI 1534 GTAGAC GT^[AC][GT]AC
AccII 3026 CGCG CG^CG
AhdI 2671 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3544 GTGCAC G^TGCAC
Aor51HI 1165 AGCGCT AGC^GCT
ApaLI 3544 GTGCAC G^TGCAC
AseI 1200 ATTAAT AT^TAAT
AsnI 1200 ATTAAT AT^TAAT
Asp700I 782 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 557 GGTACC G^GTACC
AspEI 2671 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 147 GGATCC G^GATCC
BanI 557 GGTACC G^G[CT][GA]CC
BcgI 723 CGATGAGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2718 AGATCT A^GATCT
BsaI 3117 GGTCTC GGTCTC(1/5)
BsaWI 2993 ACCGGA [AT]^CCGG[AT]
BsiEI 3028 CGGCCG CG[GA][CT]^CG
BsmBI 1828 GAGACG CGTCTC(1/5)
BsmI 3180 GCATTC GAATGC(1/-1)
BspMI 2663 ACCTGC ACCTGC(4/8)
BsrBI 561 CCGCTC CCGCTC(-3/-3)
BstUI 3026 CGCG CG^CG
BstXI 2091 CCAAGTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 1039 TTTAAA TTT^AAA
DrdI 2392 GACTCCTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3028 CGGCCG C^GGCCG
Eam1105I 2671 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3028 CGGCCG C^GGCCG
Eco47III 1165 AGCGCT AGC^GCT
Eco52I 3028 CGGCCG C^GGCCG
HaeII 1165 AGCGCT [GA]GCGC^[CT]
HincII 444 GTCAAC GT[CT]^[GA]AC
HindII 444 GTCAAC GT[CT]^[GA]AC
KpnI 557 GGTACC GGTAC^C
MvnI 3026 CGCG CG^CG
PshBI 1200 ATTAAT AT^TAAT
SacI 166 GAGCTC GAGCT^C
SexAI 323 ACCTGGT A^CC[AT]GGT
SmaI 3083 CCCGGG CCC^GGG
XcmI 2199 CCAGACCCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 3083 CCCGGG C^CCGGG
XmaI 3083 CCCGGG C^CCGGG
XmnI 782 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AflIII 1964 ACATGT A^C[GA][CT]GT
2579 ACATGT
AspI 322 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2880 GACCATGTC
AvaI 3083 CCCGGG C^[CT]CG[GA]G
3264 CTCGGG
BalI 2069 TGGCCA TGG^CCA
2211 TGGCCA
BbsI 238 GAAGAC GAAGAC(2/6)
319 GAAGAC
BclI 2147 TGATCA T^GATCA
2706 TGATCA
BpuAI 238 GAAGAC GAAGAC(2/6)
319 GAAGAC
BsoBI 3083 CCCGGG C^[CT]CG[GA]G
3264 CTCGGG
Bsp1407I 2582 TGTACA T^GTACA
3419 TGTACA
BspLU11I 1964 ACATGT A^CATGT
2579 ACATGT
BsrGI 2582 TGTACA T^GTACA
3419 TGTACA
DraIII 32 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2935 CACCATGTG
EcoNI 398 CCTTGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
498 CCTGGGGGAGG
FbaI 2147 TGATCA T^GATCA
2706 TGATCA
MluNI 2069 TGGCCA TGG^CCA
2211 TGGCCA
MscI 2069 TGGCCA TGG^CCA
2211 TGGCCA
NaeI 197 GCCGGC GCC^GGC
2240 GCCGGC
NgoMIV 197 GCCGGC G^CCGGC
2240 GCCGGC
PflFI 322 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2880 GACCATGTC
PstI 2355 CTGCAG CTGCA^G
3307 CTGCAG
ScaI 548 AGTACT AGT^ACT
3311 AGTACT
SspBI 2582 TGTACA T^GTACA
3419 TGTACA
StuI 395 AGGCCT AGG^CCT
3134 AGGCCT
Tsp45I 1904 GTGAC ^GT[GC]AC
2849 GTCAC
Tth111I 322 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2880 GACCATGTC
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# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
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AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI AspHI AvaII
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV NspI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII