(Circular) (Six-base) MAPSORT of: pFN21AE0395.seq Check: 875 from: 1 to: 8269 pFN21AE0395 8269 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4677 4677 Size: 8269 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6079 278 Size: 53 83 186 936 4543 2468 Fragments arranged by size: 4543 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3540 5612 1937 Size: 1603 2072 4594 Fragments arranged by size: 4594 2072 1603 Acc65I G'GTAC_C Cuts at: 2562 5590 2562 Size: 3028 5241 AclI AA'CG_TT Cuts at: 1563 6396 6769 1563 Size: 4833 373 3063 Fragments arranged by size: 4833 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2456 4430 6332 7716 7984 1632 Size: 824 1974 1902 1384 268 1917 Fragments arranged by size: 1974 1917 1902 1384 824 268 AfeI AGC'GCT Cuts at: 3172 3172 Size: 8269 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8072 AflIII A'CryG_T Cuts at: 1203 3969 4584 7168 8145 1203 Size: 2766 615 2584 977 1327 Fragments arranged by size: 2766 2584 1327 977 615 AgeI A'CCGG_T Cuts at: 7100 7100 Size: 8269 AhdI GACnn_n'nnGTC Cuts at: 4681 6998 4681 Size: 2317 5952 AleI CACnn'nnGTG Cuts at: 1166 2023 1166 Size: 857 7412 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8269 AlwNI CAG_nnn'CTG Cuts at: 2671 5359 5422 6012 7584 2671 Size: 2688 63 590 1572 3356 Fragments arranged by size: 3356 2688 1572 590 63 ApaI G_GGCC'C Cuts at: 3618 3999 4459 4797 3618 Size: 381 460 338 7090 Fragments arranged by size: 7090 460 381 338 ApaLI G'TGCA_C Cuts at: 5549 6326 7482 5549 Size: 777 1156 6336 Fragments arranged by size: 6336 1156 777 ApoI r'AATT_y Cuts at: 1493 2106 3509 4419 4957 5391 5410 5578 5804 Size: 613 1403 910 538 434 19 168 226 Cuts at: 5804 5956 8065 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1403 910 613 538 434 226 168 152 19 AseI AT'TA_AT Cuts at: 160 1032 3206 6823 160 Size: 872 2174 3617 1606 Fragments arranged by size: 3617 2174 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8269 AvaI C'yCGr_G Cuts at: 1870 1943 5088 5269 5594 1870 Size: 73 3145 181 325 4545 Fragments arranged by size: 4545 3145 325 181 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3300 3333 3300 Size: 33 8236 BamHI G'GATC_C Cuts at: 2152 5599 5966 2152 Size: 3447 367 4455 Fragments arranged by size: 4455 3447 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2562 5590 7045 618 Size: 325 217 627 775 3028 1455 1842 Fragments arranged by size: 3028 1842 1455 775 627 325 217 BanII G_rGCy'C Cuts at: 1391 2013 2175 3618 3761 3999 4459 4797 5012 Size: 622 162 1443 143 238 460 338 215 Cuts at: 5012 5588 1391 Size: 576 4072 Fragments arranged by size: 4072 1443 622 576 460 338 238 215 162 143 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2250 2331 928 Size: 971 351 81 6866 Fragments arranged by size: 6866 971 351 81 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2717 2751 6460 6494 1903 Size: 34 780 34 3709 34 3678 Fragments arranged by size: 3709 3678 780 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6161 7371 6161 Size: 1210 7059 BclI T'GATC_A Cuts at: 1548 4152 4711 1548 Size: 2604 559 5106 Fragments arranged by size: 5106 2604 559 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6880 136 Size: 107 122 71 631 605 5208 1525 Fragments arranged by size: 5208 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4723 8264 4723 Size: 3541 4728 BlpI GC'TnA_GC Cuts at: 5686 5686 Size: 8269 Bme1580I G_kGCm'C Cuts at: 1790 3618 3712 3999 4459 4797 5553 6330 7486 Size: 1828 94 287 460 338 756 777 1156 Cuts at: 7486 1790 Size: 2573 Fragments arranged by size: 2573 1828 1156 777 756 460 338 287 94 BmgBI CAC'GTC Cuts at: 1318 4267 4659 1318 Size: 2949 392 4928 Fragments arranged by size: 4928 2949 392 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2545 3098 4002 Size: 416 53 83 399 24 1128 553 904 Cuts at: 4002 4813 5550 6000 6958 442 Size: 811 737 450 958 1753 Fragments arranged by size: 1753 1128 958 904 811 737 553 450 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8269 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2032 5158 2032 Size: 3126 5143 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2006 2159 4102 4973 5072 5266 6929 1239 Size: 767 153 1943 871 99 194 1663 2579 Fragments arranged by size: 2579 1943 1663 871 767 194 153 99 Bpu10I CC'TnA_GC Cuts at: 3459 3459 Size: 8269 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2471 3914 5499 6394 7259 7557 2471 Size: 1443 1585 895 865 298 3183 Fragments arranged by size: 3183 1585 1443 895 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5128 6932 882 Size: 617 3629 1804 2219 Fragments arranged by size: 3629 2219 1804 617 BsaAI yAC'GTr Cuts at: 493 1204 6060 493 Size: 711 4856 2702 Fragments arranged by size: 4856 2702 711 BsaBI GATnn'nnATC Cuts at: 8263 8263 Size: 8269 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6076 6458 7093 275 Size: 53 83 186 936 4543 382 635 1451 Fragments arranged by size: 4543 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4998 6702 7100 7374 7521 7932 4998 Size: 1704 398 274 147 411 5335 Fragments arranged by size: 5335 1704 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3612 3642 5248 5278 1178 Size: 30 165 30 2209 30 1606 30 4169 Fragments arranged by size: 4169 2209 1606 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3611 3630 5215 1186 Size: 2425 19 1585 4240 Fragments arranged by size: 4240 2425 1585 19 BseYI C'CCAG_C Cuts at: 3469 3791 3901 5294 5535 7472 7968 3469 Size: 322 110 1393 241 1937 496 3770 Fragments arranged by size: 3770 1937 1393 496 322 241 110 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2974 3865 4649 4979 1163 Size: 573 1238 891 784 330 4453 Fragments arranged by size: 4453 1238 891 784 573 330 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5036 5719 6480 7508 1540 Size: 275 186 3035 683 761 1028 2301 Fragments arranged by size: 3035 2301 1028 761 683 275 186 BsiHKAI G_wGCw'C Cuts at: 2175 3167 3640 4542 5553 6330 6415 7486 2175 Size: 992 473 902 1011 777 85 1071 2958 Fragments arranged by size: 2958 1071 1011 992 902 777 473 85 BsmI GAATG_Cn' Cuts at: 5185 5780 5873 5185 Size: 595 93 7581 Fragments arranged by size: 7581 595 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3827 3827 Size: 8269 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2175 3167 3618 3640 3712 3761 Size: 399 223 162 992 451 22 72 49 Cuts at: 3761 3999 4459 4542 4797 5012 5553 5588 6330 Size: 238 460 83 255 215 541 35 742 Cuts at: 6330 6415 7486 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 992 742 541 460 451 399 255 238 223 215 162 85 83 72 49 35 22 BspEI T'CCGG_A Cuts at: 7932 7932 Size: 8269 BspHI T'CATG_A Cuts at: 6158 7888 6158 Size: 1730 6539 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4677 5624 844 Size: 3833 947 3489 Fragments arranged by size: 3833 3489 947 BsrBI CCG'CTC Cuts at: 1394 1823 2568 6156 1394 Size: 429 745 3588 3507 Fragments arranged by size: 3588 3507 745 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2289 2640 3870 6764 6938 65 Size: 1183 1041 351 1230 2894 174 1396 Fragments arranged by size: 2894 1396 1230 1183 1041 351 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2202 2559 4245 4757 6913 7100 1700 Size: 110 392 357 1686 512 2156 187 2869 Fragments arranged by size: 2869 2156 1686 512 392 357 187 110 BsrGI T'GTAC_A Cuts at: 96 4587 5424 96 Size: 4491 837 2941 Fragments arranged by size: 4491 2941 837 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8269 BssSI C'ACGA_G Cuts at: 6329 7341 6329 Size: 1012 7257 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8269 BstBI TT'CG_AA Cuts at: 7073 7073 Size: 8269 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8269 BstXI CCAn_nnnn'nTGG Cuts at: 4103 4103 Size: 8269 BstYI r'GATC_y Cuts at: 1972 2152 2667 4348 4723 5599 5966 6351 6368 Size: 180 515 1681 375 876 367 385 17 Cuts at: 6368 7820 7906 7918 8264 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1681 1452 876 515 385 375 367 346 180 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2272 3858 4012 4213 513 Size: 552 939 268 1586 154 201 4569 Fragments arranged by size: 4569 1586 939 552 268 201 154 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2742 487 Size: 683 56 1516 6014 Fragments arranged by size: 6014 1516 683 56 BtsI GCAGTG_nn' Cuts at: 2652 3913 5100 5785 6579 6599 2652 Size: 1261 1187 685 794 20 4322 Fragments arranged by size: 4322 1261 1187 794 685 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8269 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4131 4166 529 Size: 35 3567 35 4632 Fragments arranged by size: 4632 3567 35 35 DraI TTT'AAA Cuts at: 3046 5573 3046 Size: 2527 5742 DraIII CAC_nnn'GTG Cuts at: 2042 4945 2042 Size: 2903 5366 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4403 7276 809 Size: 507 3087 2873 1802 Fragments arranged by size: 3087 2873 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4074 4216 4247 Size: 54 1425 50 275 18 2244 142 31 Cuts at: 4247 5033 5716 6605 8234 8 Size: 786 683 889 1629 43 Fragments arranged by size: 2244 1629 1425 889 786 683 275 142 54 50 43 31 18 EagI C'GGCC_G Cuts at: 1537 1812 5033 5716 1537 Size: 275 3221 683 4090 Fragments arranged by size: 4090 3221 683 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2184 2782 2943 4290 6199 1353 Size: 831 598 161 1347 1909 3423 Fragments arranged by size: 3423 1909 1347 831 598 161 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5211 6856 7230 7376 387 Size: 1064 138 3622 1645 374 146 1280 Fragments arranged by size: 3622 1645 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2173 2173 Size: 8269 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2006 2159 2456 4102 4430 4973 5072 Size: 393 374 153 297 1646 328 543 99 Cuts at: 5072 5266 6332 6929 7716 7984 1239 Size: 194 1066 597 787 268 1524 Fragments arranged by size: 1646 1524 1066 787 597 543 393 374 328 297 268 194 153 99 EcoNI CCTnn'n_nnAGG Cuts at: 2407 2507 2407 Size: 100 8169 EcoO109I rG'GnC_Cy Cuts at: 3614 3946 4455 4456 4765 4793 4794 4895 5470 Size: 332 509 1 309 28 1 101 575 Cuts at: 5470 3614 Size: 6413 Fragments arranged by size: 6413 575 509 332 309 101 28 1 1 EcoRI G'AATT_C Cuts at: 5578 5956 5578 Size: 378 7891 FspI TGC'GCA Cuts at: 6775 6775 Size: 8269 HaeII r_GCGC'y Cuts at: 1678 3174 7416 1678 Size: 1496 4242 2531 Fragments arranged by size: 4242 2531 1496 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2582 2614 2852 2884 3392 Size: 32 135 32 1010 32 238 32 508 Cuts at: 3392 3424 4484 4516 4667 4699 5398 5430 6910 Size: 32 1060 32 151 32 699 32 1480 Cuts at: 6910 6942 6984 7016 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1480 1060 1010 699 508 238 151 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2451 5613 5859 669 Size: 988 281 513 3162 246 3079 Fragments arranged by size: 3162 3079 988 513 281 246 HindIII A'AGCT_T Cuts at: 748 2318 4085 5213 748 Size: 1570 1767 1128 3804 Fragments arranged by size: 3804 1767 1570 1128 HpaI GTT'AAC Cuts at: 5859 5859 Size: 8269 KpnI G_GTAC'C Cuts at: 2566 5594 2566 Size: 3028 5241 MfeI C'AATT_G Cuts at: 5868 5868 Size: 8269 MluI A'CGCG_T Cuts at: 8145 8145 Size: 8269 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2841 3600 7383 7567 1305 Size: 1536 759 3783 184 2007 Fragments arranged by size: 3783 2007 1536 759 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4076 4218 10 Size: 54 1425 343 2244 142 4061 Fragments arranged by size: 4061 2244 1425 343 142 54 MslI CAynn'nnrTG Cuts at: 518 1166 2023 2645 3135 4886 6227 6586 6745 Size: 648 857 622 490 1751 1341 359 159 Cuts at: 6745 518 Size: 2042 Fragments arranged by size: 2042 1751 1341 857 648 622 490 359 159 MspA1I CmG'CkG Cuts at: 1606 2179 3817 3901 4359 5422 5539 5685 6362 Size: 573 1638 84 458 1063 117 146 677 Cuts at: 6362 7510 7755 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1638 1148 1063 677 573 458 245 146 117 84 NaeI GCC'GGC Cuts at: 2204 4247 2204 Size: 2043 6226 NcoI C'CATG_G Cuts at: 513 1065 2004 2272 3858 4012 4213 513 Size: 552 939 268 1586 154 201 4569 Fragments arranged by size: 4569 1586 939 552 268 201 154 NdeI CA'TA_TG Cuts at: 387 3217 4233 5464 387 Size: 2830 1016 1231 3192 Fragments arranged by size: 3192 2830 1231 1016 NgoMIV G'CCGG_C Cuts at: 2202 4245 2202 Size: 2043 6226 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8269 NotI GC'GGCC_GC Cuts at: 5716 5716 Size: 8269 NspI r_CATG'y Cuts at: 1132 1229 3973 4477 4588 5627 7172 1132 Size: 97 2744 504 111 1039 1545 2229 Fragments arranged by size: 2744 2229 1545 1039 504 111 97 PasI CC'CwG_GG Cuts at: 1348 2254 2632 3674 1348 Size: 906 378 1042 5943 Fragments arranged by size: 5943 1042 906 378 PciI A'CATG_T Cuts at: 3969 4584 7168 3969 Size: 615 2584 5070 Fragments arranged by size: 5070 2584 615 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8269 PfoI T'CCnGG_A Cuts at: 2586 5252 2586 Size: 2666 5603 PmeI GTTT'AAAC Cuts at: 5573 5573 Size: 8269 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6338 6370 7851 7883 6338 Size: 32 1481 32 6724 Fragments arranged by size: 6724 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3946 4765 5470 3946 Size: 819 705 6745 Fragments arranged by size: 6745 819 705 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8269 PsiI TTA'TAA Cuts at: 2991 5839 2991 Size: 2848 5421 PspOMI G'GGCC_C Cuts at: 3614 3995 4455 4793 3614 Size: 381 460 338 7090 Fragments arranged by size: 7090 460 381 338 PstI C_TGCA'G Cuts at: 830 4364 5316 5621 830 Size: 3534 952 305 3478 Fragments arranged by size: 3534 3478 952 305 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8269 PvuII CAG'CTG Cuts at: 2179 3901 5422 5539 2179 Size: 1722 1521 117 4909 Fragments arranged by size: 4909 1722 1521 117 SacI G_AGCT'C Cuts at: 2175 2175 Size: 8269 SalI G'TCGA_C Cuts at: 1936 5611 1936 Size: 3675 4594 SbfI CC_TGCA'GG Cuts at: 5621 5621 Size: 8269 ScaI AGT'ACT Cuts at: 2555 5318 6517 8229 2555 Size: 2763 1199 1712 2595 Fragments arranged by size: 2763 2595 1712 1199 SexAI A'CCwGG_T Cuts at: 2328 2328 Size: 8269 SfcI C'TryA_G Cuts at: 826 1045 4360 4525 4719 5312 5451 5617 6752 Size: 219 3315 165 194 593 139 166 1135 Cuts at: 6752 7433 7624 826 Size: 681 191 1471 Fragments arranged by size: 3315 1471 1135 681 593 219 194 191 166 165 139 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8269 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8269 SmaI CCC'GGG Cuts at: 1872 5090 5596 1872 Size: 3218 506 4545 Fragments arranged by size: 4545 3218 506 SmlI C'TyrA_G Cuts at: 820 1017 1943 2486 3929 5514 6373 7274 7536 Size: 197 926 543 1443 1585 859 901 262 Cuts at: 7536 820 Size: 1553 Fragments arranged by size: 1585 1553 1443 926 901 859 543 262 197 SnaBI TAC'GTA Cuts at: 493 6060 493 Size: 5567 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8269 SphI G_CATG'C Cuts at: 1132 5627 1132 Size: 4495 3774 SrfI GCCC'GGGC Cuts at: 5090 5090 Size: 8269 SspI AAT'ATT Cuts at: 5 52 6193 5 Size: 47 6141 2081 Fragments arranged by size: 6141 2081 47 StuI AGG'CCT Cuts at: 1517 2402 5141 1517 Size: 885 2739 4645 Fragments arranged by size: 4645 2739 885 StyI C'CwwG_G Cuts at: 513 1065 2004 2116 2272 2403 2847 3568 3666 Size: 552 939 112 156 131 444 721 98 Cuts at: 3666 3858 4012 4213 5115 5708 513 Size: 192 154 201 902 593 3074 Fragments arranged by size: 3074 939 902 721 593 552 444 201 192 156 154 131 112 98 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4894 5001 6296 6313 6466 6651 7096 Size: 1255 3314 107 1295 17 153 185 445 Cuts at: 7096 325 Size: 1498 Fragments arranged by size: 3314 1498 1295 1255 445 185 153 107 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2439 2553 2673 Size: 274 80 33 51 1443 462 114 120 Cuts at: 2673 4587 5316 5424 5558 6515 8227 96 Size: 1914 729 108 134 957 1712 138 Fragments arranged by size: 1914 1712 1443 957 729 462 274 138 134 120 114 108 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3612 3644 4172 4204 8229 8261 3612 Size: 32 528 32 4025 32 3620 Fragments arranged by size: 4025 3620 528 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2330 4888 1316 Size: 1014 2558 4697 Fragments arranged by size: 4697 2558 1014 XbaI T'CTAG_A Cuts at: 5605 5605 Size: 8269 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4211 5563 4211 Size: 1352 6917 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8269 XmaI C'CCGG_G Cuts at: 1870 5088 5594 1870 Size: 3218 506 4545 Fragments arranged by size: 4545 3218 506 XmnI GAAnn'nnTTC Cuts at: 2791 6398 2791 Size: 3607 4662 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6077 276 Size: 53 83 186 936 4543 2468 Fragments arranged by size: 4543 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbeI BbvCI BfrBI BsiWI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SwaI