(Circular) (Six-base) MAPSORT of: pFN21AE0394.seq Check: 9608 from: 1 to: 8065 pFN21AE0394 8065 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5875 278 Size: 53 83 186 936 4339 2468 Fragments arranged by size: 4339 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5408 1937 Size: 3471 4594 Acc65I G'GTAC_C Cuts at: 3616 3733 5386 3616 Size: 117 1653 6295 Fragments arranged by size: 6295 1653 117 AclI AA'CG_TT Cuts at: 1563 6192 6565 1563 Size: 4629 373 3063 Fragments arranged by size: 4629 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3989 4212 6128 7512 7780 1632 Size: 2357 223 1916 1384 268 1917 Fragments arranged by size: 2357 1917 1916 1384 268 223 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7868 AflIII A'CryG_T Cuts at: 1203 2290 3422 4961 5064 6964 7941 1203 Size: 1087 1132 1539 103 1900 977 1327 Fragments arranged by size: 1900 1539 1327 1132 1087 977 103 AgeI A'CCGG_T Cuts at: 6896 6896 Size: 8065 AhdI GACnn_n'nnGTC Cuts at: 6794 6794 Size: 8065 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8065 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4164 1184 Size: 2980 5085 AlwNI CAG_nnn'CTG Cuts at: 4300 4942 5808 7380 4300 Size: 642 866 1572 4985 Fragments arranged by size: 4985 1572 866 642 ApaI G_GGCC'C Cuts at: 3585 4503 3585 Size: 918 7147 ApaLI G'TGCA_C Cuts at: 3139 6122 7278 3139 Size: 2983 1156 3926 Fragments arranged by size: 3926 2983 1156 ApoI r'AATT_y Cuts at: 1493 2471 2605 3567 4401 4409 4706 5374 5600 Size: 978 134 962 834 8 297 668 226 Cuts at: 5600 5752 7861 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 978 962 834 668 297 226 152 134 8 AseI AT'TA_AT Cuts at: 160 1032 2439 2523 6619 160 Size: 872 1407 84 4096 1606 Fragments arranged by size: 4096 1606 1407 872 84 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8065 AvaI C'yCGr_G Cuts at: 1870 1943 2991 3531 5390 1870 Size: 73 1048 540 1859 4545 Fragments arranged by size: 4545 1859 1048 540 73 BamHI G'GATC_C Cuts at: 5395 5762 5395 Size: 367 7698 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3382 3616 3733 5203 5386 Size: 325 217 627 1595 234 117 1470 183 Cuts at: 5386 6841 618 Size: 1455 1842 Fragments arranged by size: 1842 1595 1470 1455 627 325 234 217 183 117 BanII G_rGCy'C Cuts at: 1391 2992 3396 3585 4503 5384 1391 Size: 1601 404 189 918 881 4072 Fragments arranged by size: 4072 1601 918 881 404 189 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4394 928 Size: 971 2495 4599 Fragments arranged by size: 4599 2495 971 BbvCI CC'TCA_GC Cuts at: 3897 4935 3897 Size: 1038 7027 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6256 6290 1903 Size: 34 4319 34 3678 Fragments arranged by size: 4319 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5957 7167 5957 Size: 1210 6855 BclI T'GATC_A Cuts at: 1548 2265 2854 1548 Size: 717 589 6759 Fragments arranged by size: 6759 717 589 BfrBI ATG'CAT Cuts at: 2343 2343 Size: 8065 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2157 2996 6676 Size: 107 122 71 631 605 485 839 3680 Cuts at: 6676 136 Size: 1525 Fragments arranged by size: 3680 1525 839 631 605 485 122 107 71 BglII A'GATC_T Cuts at: 8060 8060 Size: 8065 BlpI GC'TnA_GC Cuts at: 5482 5482 Size: 8065 Bme1580I G_kGCm'C Cuts at: 1790 3143 3585 4503 6126 7282 1790 Size: 1353 442 918 1623 1156 2573 Fragments arranged by size: 2573 1623 1353 1156 918 442 BmgBI CAC'GTC Cuts at: 1318 3329 1318 Size: 2011 6054 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5181 5796 6754 Size: 416 53 83 399 24 3764 615 958 Cuts at: 6754 442 Size: 1753 Fragments arranged by size: 3764 1753 958 615 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8065 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2024 2128 2268 2656 2667 3213 3476 3723 Size: 785 104 140 388 11 546 263 247 Cuts at: 3723 4445 6725 1239 Size: 722 2280 2579 Fragments arranged by size: 2579 2280 785 722 546 388 263 247 140 104 11 Bpu10I CC'TnA_GC Cuts at: 3270 3897 4935 3270 Size: 627 1038 6400 Fragments arranged by size: 6400 1038 627 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2486 3165 3291 3620 6190 7055 7353 2486 Size: 679 126 329 2570 865 298 3198 Fragments arranged by size: 3198 2570 865 679 329 298 126 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6728 882 Size: 617 5229 2219 Fragments arranged by size: 5229 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2293 5856 493 Size: 711 1089 3563 2702 Fragments arranged by size: 3563 2702 1089 711 BsaBI GATnn'nnATC Cuts at: 4049 8059 4049 Size: 4010 4055 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5872 6254 6889 275 Size: 53 83 186 936 4339 382 635 1451 Fragments arranged by size: 4339 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2425 6498 6896 7170 7317 7728 2425 Size: 4073 398 274 147 411 2762 Fragments arranged by size: 4073 2762 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2610 2640 3474 3504 3960 Size: 30 165 30 1207 30 834 30 456 Cuts at: 3960 3990 1178 Size: 30 5253 Fragments arranged by size: 5253 1207 834 456 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2270 3452 3743 4670 5231 1186 Size: 1084 1182 291 927 561 4020 Fragments arranged by size: 4020 1182 1084 927 561 291 BseYI C'CCAG_C Cuts at: 7268 7764 7268 Size: 496 7569 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7492 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4996 5515 6276 7304 1540 Size: 275 186 2995 519 761 1028 2301 Fragments arranged by size: 2995 2301 1028 761 519 275 186 BsiHKAI G_wGCw'C Cuts at: 2013 2642 2745 3143 3492 3682 3975 6126 6211 Size: 629 103 398 349 190 293 2151 85 Cuts at: 6211 7282 2013 Size: 1071 2796 Fragments arranged by size: 2796 2151 1071 629 398 349 293 190 103 85 BsmI GAATG_Cn' Cuts at: 5576 5669 5576 Size: 93 7972 BsmBI CGTCTCn'nnnn_ Cuts at: 3319 3319 Size: 8065 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2642 2745 2992 3143 3396 3492 Size: 399 223 629 103 247 151 253 96 Cuts at: 3492 3585 3682 3975 4503 5384 6126 6211 7282 Size: 93 97 293 528 881 742 85 1071 Cuts at: 7282 1391 Size: 2174 Fragments arranged by size: 2174 1071 881 742 629 528 399 293 253 247 223 151 103 97 96 93 85 BspEI T'CCGG_A Cuts at: 7728 7728 Size: 8065 BspHI T'CATG_A Cuts at: 2844 5954 7684 2844 Size: 3110 1730 3225 Fragments arranged by size: 3225 3110 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5420 844 Size: 4576 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5952 1394 Size: 429 4129 3507 Fragments arranged by size: 4129 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2137 6560 6734 65 Size: 1183 889 4423 174 1396 Fragments arranged by size: 4423 1396 1183 889 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6709 6896 1700 Size: 110 4899 187 2869 Fragments arranged by size: 4899 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2884 4956 96 Size: 2788 2072 3205 Fragments arranged by size: 3205 2788 2072 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8065 BssSI C'ACGA_G Cuts at: 6125 7137 6125 Size: 1012 7053 BstAPI GCAn_nnn'nTGC Cuts at: 794 3266 3677 794 Size: 2472 411 5182 Fragments arranged by size: 5182 2472 411 BstBI TT'CG_AA Cuts at: 3840 6869 3840 Size: 3029 5036 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8065 BstYI r'GATC_y Cuts at: 1972 2196 5395 5762 6147 6164 7616 7702 7714 Size: 224 3199 367 385 17 1452 86 12 Cuts at: 7714 8060 1972 Size: 346 1977 Fragments arranged by size: 3199 1977 1452 385 367 346 224 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2092 513 Size: 552 939 88 6486 Fragments arranged by size: 6486 939 552 88 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4024 487 Size: 683 56 2798 4528 Fragments arranged by size: 4528 2798 683 56 BtsI GCAGTG_nn' Cuts at: 2049 3222 4919 5581 6375 6395 2049 Size: 1173 1697 662 794 20 3719 Fragments arranged by size: 3719 1697 1173 794 662 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8065 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3835 3870 529 Size: 35 3271 35 4724 Fragments arranged by size: 4724 3271 35 35 DraI TTT'AAA Cuts at: 5369 5369 Size: 8065 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7072 809 Size: 507 5756 1802 Fragments arranged by size: 5756 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4974 5145 5512 Size: 54 1425 50 275 18 3144 171 367 Cuts at: 5512 6401 8030 8 Size: 889 1629 43 Fragments arranged by size: 3144 1629 1425 889 367 275 171 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5512 1537 Size: 275 3700 4090 Fragments arranged by size: 4090 3700 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3550 4817 5123 5995 1353 Size: 2197 1267 306 872 3423 Fragments arranged by size: 3423 2197 1267 872 306 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6652 7026 7172 387 Size: 1064 138 5063 374 146 1280 Fragments arranged by size: 5063 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2024 2128 2268 2656 2667 3213 3476 Size: 393 392 104 140 388 11 546 263 Cuts at: 3476 3723 3989 4212 4445 6128 6725 7512 7780 Size: 247 266 223 233 1683 597 787 268 Cuts at: 7780 1239 Size: 1524 Fragments arranged by size: 1683 1524 787 597 546 393 392 388 268 266 263 247 233 223 140 104 11 EcoNI CCTnn'n_nnAGG Cuts at: 4875 4875 Size: 8065 EcoO109I rG'GnC_Cy Cuts at: 3581 4696 3581 Size: 1115 6950 EcoRI G'AATT_C Cuts at: 4409 5374 5752 4409 Size: 965 378 6722 Fragments arranged by size: 6722 965 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2426 4019 2426 Size: 1593 6472 FspI TGC'GCA Cuts at: 6571 6571 Size: 8065 HaeII r_GCGC'y Cuts at: 1678 4942 7212 1678 Size: 3264 2270 2531 Fragments arranged by size: 3264 2531 2270 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2266 2288 2298 2320 2573 Size: 32 135 32 694 22 10 22 253 Cuts at: 2573 2605 4074 4106 4790 4822 5011 5043 6706 Size: 32 1469 32 684 32 189 32 1663 Cuts at: 6706 6738 6780 6812 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1663 1469 694 684 253 189 135 42 32 32 32 32 32 32 32 32 22 22 10 HincII GTy'rAC Cuts at: 669 1657 1938 2089 4111 4207 4341 4465 5409 Size: 988 281 151 2022 96 134 124 944 Cuts at: 5409 5655 669 Size: 246 3079 Fragments arranged by size: 3079 2022 988 944 281 246 151 134 124 96 HindIII A'AGCT_T Cuts at: 748 2403 3359 4566 5101 748 Size: 1655 956 1207 535 3712 Fragments arranged by size: 3712 1655 1207 956 535 HpaI GTT'AAC Cuts at: 5655 5655 Size: 8065 KpnI G_GTAC'C Cuts at: 3620 3737 5390 3620 Size: 117 1653 6295 Fragments arranged by size: 6295 1653 117 MfeI C'AATT_G Cuts at: 5664 5664 Size: 8065 MluI A'CGCG_T Cuts at: 7941 7941 Size: 8065 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4648 7179 7363 1305 Size: 3343 2531 184 2007 Fragments arranged by size: 3343 2531 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4976 5147 10 Size: 54 1425 343 3144 171 2928 Fragments arranged by size: 3144 2928 1425 343 171 54 MslI CAynn'nnrTG Cuts at: 518 1166 2348 3885 4053 6023 6382 6541 518 Size: 648 1182 1537 168 1970 359 159 2042 Fragments arranged by size: 2042 1970 1537 1182 648 359 168 159 MspA1I CmG'CkG Cuts at: 1606 2321 3116 4559 5481 6158 7306 7551 1606 Size: 715 795 1443 922 677 1148 245 2120 Fragments arranged by size: 2120 1443 1148 922 795 715 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2092 513 Size: 552 939 88 6486 Fragments arranged by size: 6486 939 552 88 NdeI CA'TA_TG Cuts at: 387 387 Size: 8065 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8065 NotI GC'GGCC_GC Cuts at: 5512 5512 Size: 8065 NsiI A_TGCA'T Cuts at: 2345 2345 Size: 8065 NspI r_CATG'y Cuts at: 1132 1229 3353 3426 4911 4965 5068 5333 5423 Size: 97 2124 73 1485 54 103 265 90 Cuts at: 5423 6968 1132 Size: 1545 2229 Fragments arranged by size: 2229 2124 1545 1485 265 103 97 90 73 54 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8065 PciI A'CATG_T Cuts at: 3422 4961 5064 6964 3422 Size: 1539 103 1900 4523 Fragments arranged by size: 4523 1900 1539 103 PflMI CCAn_nnn'nTGG Cuts at: 1108 3644 4300 1108 Size: 2536 656 4873 Fragments arranged by size: 4873 2536 656 PfoI T'CCnGG_A Cuts at: 2284 2895 3492 3739 2284 Size: 611 597 247 6610 Fragments arranged by size: 6610 611 597 247 PmeI GTTT'AAAC Cuts at: 5369 5369 Size: 8065 PmlI CAC'GTG Cuts at: 2293 2293 Size: 8065 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6134 6166 7647 7679 6134 Size: 32 1481 32 6520 Fragments arranged by size: 6520 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4696 4696 Size: 8065 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8065 PsiI TTA'TAA Cuts at: 5635 5635 Size: 8065 PspOMI G'GGCC_C Cuts at: 3581 4499 3581 Size: 918 7147 PstI C_TGCA'G Cuts at: 830 5417 830 Size: 4587 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8065 PvuII CAG'CTG Cuts at: 3116 4559 3116 Size: 1443 6622 SalI G'TCGA_C Cuts at: 1936 5407 1936 Size: 3471 4594 SbfI CC_TGCA'GG Cuts at: 5417 5417 Size: 8065 ScaI AGT'ACT Cuts at: 6313 8025 6313 Size: 1712 6353 SfcI C'TryA_G Cuts at: 826 1045 2758 5413 6548 7229 7420 826 Size: 219 1713 2655 1135 681 191 1471 Fragments arranged by size: 2655 1713 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8065 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8065 SmaI CCC'GGG Cuts at: 1872 2993 5392 1872 Size: 1121 2399 4545 Fragments arranged by size: 4545 2399 1121 SmlI C'TyrA_G Cuts at: 820 1017 1943 2501 3180 3306 3599 6169 7070 Size: 197 926 558 679 126 293 2570 901 Cuts at: 7070 7332 820 Size: 262 1553 Fragments arranged by size: 2570 1553 926 901 679 558 293 262 197 126 SnaBI TAC'GTA Cuts at: 493 5856 493 Size: 5363 2702 SpeI A'CTAG_T Cuts at: 152 4510 5158 152 Size: 4358 648 3059 Fragments arranged by size: 4358 3059 648 SphI G_CATG'C Cuts at: 1132 3353 5333 5423 1132 Size: 2221 1980 90 3774 Fragments arranged by size: 3774 2221 1980 90 SspI AAT'ATT Cuts at: 5 52 2068 2509 5989 5 Size: 47 2016 441 3480 2081 Fragments arranged by size: 3480 2081 2016 441 47 StuI AGG'CCT Cuts at: 1517 2489 3275 3298 3765 4814 1517 Size: 972 786 23 467 1049 4768 Fragments arranged by size: 4768 1049 972 786 467 23 StyI C'CwwG_G Cuts at: 513 1065 2004 2092 3293 3387 5504 513 Size: 552 939 88 1201 94 2117 3074 Fragments arranged by size: 3074 2117 1201 939 552 94 88 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2712 3413 4307 4658 4982 5191 6092 Size: 1255 1132 701 894 351 324 209 901 Cuts at: 6092 6109 6262 6447 6892 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1132 901 894 701 445 351 324 209 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2884 4956 5271 Size: 274 80 33 51 1443 907 2072 315 Cuts at: 5271 6311 8023 96 Size: 1040 1712 138 Fragments arranged by size: 2072 1712 1443 1040 907 315 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2056 2088 2685 2717 8025 8057 2056 Size: 32 597 32 5308 32 2064 Fragments arranged by size: 5308 2064 597 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4115 1316 Size: 2799 5266 XbaI T'CTAG_A Cuts at: 3724 5401 3724 Size: 1677 6388 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8065 XmaI C'CCGG_G Cuts at: 1870 2991 5390 1870 Size: 1121 2399 4545 Fragments arranged by size: 4545 2399 1121 XmnI GAAnn'nnTTC Cuts at: 4530 6194 4530 Size: 1664 6401 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5873 276 Size: 53 83 186 936 4339 2468 Fragments arranged by size: 4339 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI