Restriction map for
pFN21AE0394
(Linear) (Six-base) MAPSORT of: KIEE0394.seq Check: 4038 from: 1 to: 3360
KIEE0394.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
Acc65I G'GTAC_C
Cuts at: 0 1611 1728 3360
Size: 1611 117 1632
Fragments arranged by size:
1632 1611 117
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1984 2207 3360
Size: 1984 223 1153
Fragments arranged by size:
1984 1153 223
AflIII A'CryG_T
Cuts at: 0 285 1417 2956 3059 3360
Size: 285 1132 1539 103 301
Fragments arranged by size:
1539 1132 301 285 103
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2159 3360
Size: 2159 1201
AlwNI CAG_nnn'CTG
Cuts at: 0 2295 2937 3360
Size: 2295 642 423
Fragments arranged by size:
2295 642 423
ApaI G_GGCC'C
Cuts at: 0 1580 2498 3360
Size: 1580 918 862
Fragments arranged by size:
1580 918 862
ApaLI G'TGCA_C
Cuts at: 0 1134 3360
Size: 1134 2226
ApoI r'AATT_y
Cuts at: 0 466 600 1562 2396 2404 2701 3360
Size: 466 134 962 834 8 297 659
Fragments arranged by size:
962 834 659 466 297 134 8
AseI AT'TA_AT
Cuts at: 0 434 518 3360
Size: 434 84 2842
Fragments arranged by size:
2842 434 84
AvaI C'yCGr_G
Cuts at: 0 986 1526 3360
Size: 986 540 1834
Fragments arranged by size:
1834 986 540
BanI G'GyrC_C
Cuts at: 0 1377 1611 1728 3198 3360
Size: 1377 234 117 1470 162
Fragments arranged by size:
1470 1377 234 162 117
BanII G_rGCy'C
Cuts at: 0 987 1391 1580 2498 3360
Size: 987 404 189 918 862
Fragments arranged by size:
987 918 862 404 189
BbsI GAAGACnn'nnnn_
Cuts at: 0 2389 3360
Size: 2389 971
BbvCI CC'TCA_GC
Cuts at: 0 1892 2930 3360
Size: 1892 1038 430
Fragments arranged by size:
1892 1038 430
BclI T'GATC_A
Cuts at: 0 260 849 3360
Size: 260 589 2511
Fragments arranged by size:
2511 589 260
BfrBI ATG'CAT
Cuts at: 0 338 3360
Size: 338 3022
BglI GCCn_nnn'nGGC
Cuts at: 0 152 991 3360
Size: 152 839 2369
Fragments arranged by size:
2369 839 152
Bme1580I G_kGCm'C
Cuts at: 0 1138 1580 2498 3360
Size: 1138 442 918 862
Fragments arranged by size:
1138 918 862 442
BmgBI CAC'GTC
Cuts at: 0 1324 3360
Size: 1324 2036
BmrI ACTGGGnnnn_n'
Cuts at: 0 3176 3360
Size: 3176 184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 123 263 651 662 1208 1471 1718
Size: 19 104 140 388 11 546 263 247
Cuts at: 1718 2440 3360
Size: 722 920
Fragments arranged by size:
920 722 546 388 263 247 140 104
19 11
Bpu10I CC'TnA_GC
Cuts at: 0 1265 1892 2930 3360
Size: 1265 627 1038 430
Fragments arranged by size:
1265 1038 627 430
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 1160 1286 1615 3360
Size: 481 679 126 329 1745
Fragments arranged by size:
1745 679 481 329 126
BsaAI yAC'GTr
Cuts at: 0 288 3360
Size: 288 3072
BsaBI GATnn'nnATC
Cuts at: 0 2044 3360
Size: 2044 1316
BsaWI w'CCGG_w
Cuts at: 0 420 3360
Size: 420 2940
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 605 635 1469 1499 1955 1985 3360
Size: 605 30 834 30 456 30 1375
Fragments arranged by size:
1375 834 605 456 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 265 1447 1738 2665 3226 3360
Size: 265 1182 291 927 561 134
Fragments arranged by size:
1182 927 561 291 265 134
BsiEI CG_ry'CG
Cuts at: 0 2991 3360
Size: 2991 369
BsiHKAI G_wGCw'C
Cuts at: 0 8 637 740 1138 1487 1677 1970 3360
Size: 8 629 103 398 349 190 293 1390
Fragments arranged by size:
1390 629 398 349 293 190 103 8
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1314 3360
Size: 1314 2046
Bsp1286I G_dGCh'C
Cuts at: 0 8 637 740 987 1138 1391 1487 1580
Size: 8 629 103 247 151 253 96 93
Cuts at: 1580 1677 1970 2498 3360
Size: 97 293 528 862
Fragments arranged by size:
862 629 528 293 253 247 151 103
97 96 93 8
BspHI T'CATG_A
Cuts at: 0 839 3360
Size: 839 2521
BsrDI GCAATG_nn'
Cuts at: 0 132 3360
Size: 132 3228
BsrGI T'GTAC_A
Cuts at: 0 879 2951 3360
Size: 879 2072 409
Fragments arranged by size:
2072 879 409
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1261 1672 3360
Size: 1261 411 1688
Fragments arranged by size:
1688 1261 411
BstBI TT'CG_AA
Cuts at: 0 1835 3360
Size: 1835 1525
BstYI r'GATC_y
Cuts at: 0 191 3360
Size: 191 3169
BtgI C'CryG_G
Cuts at: 0 87 3360
Size: 87 3273
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2019 3360
Size: 2019 1341
BtsI GCAGTG_nn'
Cuts at: 0 44 1217 2914 3360
Size: 44 1173 1697 446
Fragments arranged by size:
1697 1173 446 44
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1830 1865 3360
Size: 1830 35 1495
Fragments arranged by size:
1830 1495 35
EaeI y'GGCC_r
Cuts at: 0 2969 3140 3360
Size: 2969 171 220
Fragments arranged by size:
2969 220 171
EarI CTCTTCn'nnn_
Cuts at: 0 1545 2812 3118 3360
Size: 1545 1267 306 242
Fragments arranged by size:
1545 1267 306 242
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 123 263 651 662 1208 1471 1718
Size: 19 104 140 388 11 546 263 247
Cuts at: 1718 1984 2207 2440 3360
Size: 266 223 233 920
Fragments arranged by size:
920 546 388 266 263 247 233 223
140 104 19 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2870 3360
Size: 2870 490
EcoO109I rG'GnC_Cy
Cuts at: 0 1576 2691 3360
Size: 1576 1115 669
Fragments arranged by size:
1576 1115 669
EcoRI G'AATT_C
Cuts at: 0 2404 3360
Size: 2404 956
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 421 2014 3360
Size: 421 1593 1346
Fragments arranged by size:
1593 1346 421
HaeII r_GCGC'y
Cuts at: 0 2937 3360
Size: 2937 423
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 261 283 293 315 568 600 2069 2101
Size: 261 22 10 22 253 32 1469 32
Cuts at: 2101 2785 2817 3006 3038 3360
Size: 684 32 189 32 322
Fragments arranged by size:
1469 684 322 261 253 189 32 32
32 32 22 22 10
HincII GTy'rAC
Cuts at: 0 84 2106 2202 2336 2460 3360
Size: 84 2022 96 134 124 900
Fragments arranged by size:
2022 900 134 124 96 84
HindIII A'AGCT_T
Cuts at: 0 398 1354 2561 3096 3360
Size: 398 956 1207 535 264
Fragments arranged by size:
1207 956 535 398 264
KpnI G_GTAC'C
Cuts at: 0 1615 1732 3360
Size: 1615 117 1628
Fragments arranged by size:
1628 1615 117
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2643 3360
Size: 2643 717
MscI TGG'CCA
Cuts at: 0 2971 3142 3360
Size: 2971 171 218
Fragments arranged by size:
2971 218 171
MslI CAynn'nnrTG
Cuts at: 0 343 1880 2048 3360
Size: 343 1537 168 1312
Fragments arranged by size:
1537 1312 343 168
MspA1I CmG'CkG
Cuts at: 0 316 1111 2554 3360
Size: 316 795 1443 806
Fragments arranged by size:
1443 806 795 316
NcoI C'CATG_G
Cuts at: 0 87 3360
Size: 87 3273
NsiI A_TGCA'T
Cuts at: 0 340 3360
Size: 340 3020
NspI r_CATG'y
Cuts at: 0 1348 1421 2906 2960 3063 3328 3360
Size: 1348 73 1485 54 103 265 32
Fragments arranged by size:
1485 1348 265 103 73 54 32
PciI A'CATG_T
Cuts at: 0 1417 2956 3059 3360
Size: 1417 1539 103 301
Fragments arranged by size:
1539 1417 301 103
PflMI CCAn_nnn'nTGG
Cuts at: 0 1639 2295 3360
Size: 1639 656 1065
Fragments arranged by size:
1639 1065 656
PfoI T'CCnGG_A
Cuts at: 0 279 890 1487 1734 3360
Size: 279 611 597 247 1626
Fragments arranged by size:
1626 611 597 279 247
PmlI CAC'GTG
Cuts at: 0 288 3360
Size: 288 3072
PpuMI rG'GwC_Cy
Cuts at: 0 2691 3360
Size: 2691 669
PspOMI G'GGCC_C
Cuts at: 0 1576 2494 3360
Size: 1576 918 866
Fragments arranged by size:
1576 918 866
PvuII CAG'CTG
Cuts at: 0 1111 2554 3360
Size: 1111 1443 806
Fragments arranged by size:
1443 1111 806
SfcI C'TryA_G
Cuts at: 0 753 3360
Size: 753 2607
SmaI CCC'GGG
Cuts at: 0 988 3360
Size: 988 2372
SmlI C'TyrA_G
Cuts at: 0 496 1175 1301 1594 3360
Size: 496 679 126 293 1766
Fragments arranged by size:
1766 679 496 293 126
SpeI A'CTAG_T
Cuts at: 0 2505 3153 3360
Size: 2505 648 207
Fragments arranged by size:
2505 648 207
SphI G_CATG'C
Cuts at: 0 1348 3328 3360
Size: 1348 1980 32
Fragments arranged by size:
1980 1348 32
SspI AAT'ATT
Cuts at: 0 63 504 3360
Size: 63 441 2856
Fragments arranged by size:
2856 441 63
StuI AGG'CCT
Cuts at: 0 484 1270 1293 1760 2809 3360
Size: 484 786 23 467 1049 551
Fragments arranged by size:
1049 786 551 484 467 23
StyI C'CwwG_G
Cuts at: 0 87 1288 1382 3360
Size: 87 1201 94 1978
Fragments arranged by size:
1978 1201 94 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 707 1408 2302 2653 2977 3186 3360
Size: 707 701 894 351 324 209 174
Fragments arranged by size:
894 707 701 351 324 209 174
TatI w'GTAC_w
Cuts at: 0 879 2951 3266 3360
Size: 879 2072 315 94
Fragments arranged by size:
2072 879 315 94
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 51 83 680 712 3360
Size: 51 32 597 32 2648
Fragments arranged by size:
2648 597 51 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2110 3360
Size: 2110 1250
XbaI T'CTAG_A
Cuts at: 0 1719 3360
Size: 1719 1641
XmaI C'CCGG_G
Cuts at: 0 986 3360
Size: 986 2374
XmnI GAAnn'nnTTC
Cuts at: 0 2525 3360
Size: 2525 835
Enzymes that do cut:
Acc65I AcuI AflIII AlfI AlwNI ApaI ApaLI ApoI
AseI AvaI BanI BanII BbsI BbvCI BclI BfrBI
BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaAI
BsaBI BsaWI BsaXI BseRI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BsrDI BsrGI BstAPI BstBI BstYI BtgI BtgZI
BtsI CspCI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI
FalI HaeII Hin4I HincII HindIII KpnI MmeI MscI
MslI MspA1I NcoI NsiI NspI PciI PflMI PfoI
PmlI PpuMI PspOMI PvuII SfcI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AloI AscI AsiSI AvrII BaeI BamHI BbeI
BcgI BciVI BglII BlpI BmtI BplI BsaI BsaHI
BseYI BsgI BsiWI BsmI BspEI BspMI BsrBI BsrFI
BssHII BssSI BstEII BstXI BstZ17I Bsu36I ClaI DraI
DraIII DrdI EagI EciI EcoICRI EcoRV FseI FspI
FspAI HpaI KasI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI PasI PmeI PpiI
PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SrfI SwaI XcmI XhoI
ZraI