(Circular) (Six-base) MAPSORT of: pFN21AE0393.seq Check: 8311 from: 1 to: 7570 pFN21AE0393 7570 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2651 4268 2651 Size: 1617 5953 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4731 5380 278 Size: 53 83 186 936 3195 649 2468 Fragments arranged by size: 3195 2468 936 649 186 83 53 AccI GT'mk_AC Cuts at: 1937 2067 2145 4014 4913 1937 Size: 130 78 1869 899 4594 Fragments arranged by size: 4594 1869 899 130 78 Acc65I G'GTAC_C Cuts at: 4891 4891 Size: 7570 AclI AA'CG_TT Cuts at: 1563 5697 6070 1563 Size: 4134 373 3063 Fragments arranged by size: 4134 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2621 2627 2750 2877 3083 3208 4757 5633 Size: 989 6 123 127 206 125 1549 876 Cuts at: 5633 7017 7285 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1549 1384 989 876 268 206 127 125 123 6 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7373 AflIII A'CryG_T Cuts at: 1203 2664 4413 4521 6469 7446 1203 Size: 1461 1749 108 1948 977 1327 Fragments arranged by size: 1948 1749 1461 1327 977 108 AgeI A'CCGG_T Cuts at: 6401 6401 Size: 7570 AhdI GACnn_n'nnGTC Cuts at: 2063 6299 2063 Size: 4236 3334 AleI CACnn'nnGTG Cuts at: 1166 3225 4828 1166 Size: 2059 1603 3908 Fragments arranged by size: 3908 2059 1603 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7570 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2503 2535 2503 Size: 32 7538 AlwNI CAG_nnn'CTG Cuts at: 2389 2791 3061 3658 3748 4114 4303 5313 6885 Size: 402 270 597 90 366 189 1010 1572 Cuts at: 6885 2389 Size: 3074 Fragments arranged by size: 3074 1572 1010 597 402 366 270 189 90 ApaI G_GGCC'C Cuts at: 4665 4839 4665 Size: 174 7396 ApaLI G'TGCA_C Cuts at: 2411 5627 6783 2411 Size: 3216 1156 3198 Fragments arranged by size: 3216 3198 1156 ApoI r'AATT_y Cuts at: 1493 4879 5105 5257 7366 1493 Size: 3386 226 152 2109 1697 Fragments arranged by size: 3386 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6124 160 Size: 872 5092 1606 Fragments arranged by size: 5092 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7570 AvaI C'yCGr_G Cuts at: 1870 1943 2904 3463 4437 4696 4895 1870 Size: 73 961 559 974 259 199 4545 Fragments arranged by size: 4545 974 961 559 259 199 73 BamHI G'GATC_C Cuts at: 4768 4900 5267 4768 Size: 132 367 7071 Fragments arranged by size: 7071 367 132 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2196 2350 2716 2876 3236 Size: 325 217 627 409 154 366 160 360 Cuts at: 3236 3268 3784 4394 4891 6346 618 Size: 32 516 610 497 1455 1842 Fragments arranged by size: 1842 1455 627 610 516 497 409 366 360 325 217 160 154 32 BanII G_rGCy'C Cuts at: 1391 2571 3210 3633 3872 4005 4230 4582 4665 Size: 1180 639 423 239 133 225 352 83 Cuts at: 4665 4807 4839 4889 1391 Size: 142 32 50 4072 Fragments arranged by size: 4072 1180 639 423 352 239 225 142 133 83 50 32 BbeI G_GCGC'C Cuts at: 3788 3788 Size: 7570 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2462 2969 928 Size: 971 563 507 5529 Fragments arranged by size: 5529 971 563 507 BbvCI CC'TCA_GC Cuts at: 2882 3284 2882 Size: 402 7168 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2279 2313 5761 5795 1903 Size: 34 342 34 3448 34 3678 Fragments arranged by size: 3678 3448 342 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5462 6672 5462 Size: 1210 6360 BclI T'GATC_A Cuts at: 1548 3024 1548 Size: 1476 6094 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3466 3782 6181 Size: 107 122 71 631 605 1794 316 2399 Cuts at: 6181 136 Size: 1525 Fragments arranged by size: 2399 1794 1525 631 605 316 122 107 71 BglII A'GATC_T Cuts at: 4281 7565 4281 Size: 3284 4286 BlpI GC'TnA_GC Cuts at: 4987 4987 Size: 7570 Bme1580I G_kGCm'C Cuts at: 1790 2199 2415 3093 3271 3279 3339 4146 4647 Size: 409 216 678 178 8 60 807 501 Cuts at: 4647 4665 4839 5631 6787 1790 Size: 18 174 792 1156 2573 Fragments arranged by size: 2573 1156 807 792 678 501 409 216 178 174 60 18 8 BmgBI CAC'GTC Cuts at: 1318 2380 3022 3646 1318 Size: 1062 642 624 5242 Fragments arranged by size: 5242 1062 642 624 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3627 4650 5301 Size: 416 53 83 399 24 2210 1023 651 Cuts at: 5301 6259 442 Size: 958 1753 Fragments arranged by size: 2210 1753 1023 958 651 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7570 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2058 3497 3527 4136 6230 1239 Size: 819 1439 30 609 2094 2579 Fragments arranged by size: 2579 2094 1439 819 609 30 Bpu10I CC'TnA_GC Cuts at: 2882 3177 3284 2882 Size: 295 107 7168 Fragments arranged by size: 7168 295 107 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5695 6560 6858 5695 Size: 865 298 6407 Fragments arranged by size: 6407 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2582 3898 4605 6233 882 Size: 617 1083 1316 707 1628 2219 Fragments arranged by size: 2219 1628 1316 1083 707 617 BsaAI yAC'GTr Cuts at: 493 1204 3079 3979 5361 493 Size: 711 1875 900 1382 2702 Fragments arranged by size: 2702 1875 1382 900 711 BsaBI GATnn'nnATC Cuts at: 7564 7564 Size: 7570 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3785 3813 4728 5377 Size: 53 83 186 936 2252 28 915 649 Cuts at: 5377 5759 6394 275 Size: 382 635 1451 Fragments arranged by size: 2252 1451 936 915 649 635 382 186 83 53 28 BsaWI w'CCGG_w Cuts at: 3487 6003 6401 6675 6822 7233 3487 Size: 2516 398 274 147 411 3824 Fragments arranged by size: 3824 2516 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4187 4217 4490 4520 4526 Size: 30 165 30 2784 30 273 30 6 Cuts at: 4526 4556 1178 Size: 30 4192 Fragments arranged by size: 4192 2784 273 165 30 30 30 30 30 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3295 3494 1186 Size: 2109 199 5262 Fragments arranged by size: 5262 2109 199 BseYI C'CCAG_C Cuts at: 2817 3049 3658 3705 4444 6773 7269 2817 Size: 232 609 47 739 2329 496 3118 Fragments arranged by size: 3118 2329 739 609 496 232 47 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2402 2804 2911 2918 3203 3435 3617 Size: 573 666 402 107 7 285 232 182 Cuts at: 3617 3629 3868 1163 Size: 12 239 4865 Fragments arranged by size: 4865 666 573 402 285 239 232 182 107 12 7 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2317 3416 5020 5781 6809 1540 Size: 275 186 316 1099 1604 761 1028 2301 Fragments arranged by size: 2301 1604 1099 1028 761 316 275 186 BsiHKAI G_wGCw'C Cuts at: 2415 2863 3210 3426 3675 4026 5631 5716 6787 Size: 448 347 216 249 351 1605 85 1071 Cuts at: 6787 2415 Size: 3198 Fragments arranged by size: 3198 1605 1071 448 351 347 249 216 85 BsmI GAATG_Cn' Cuts at: 4339 5081 5174 4339 Size: 742 93 6735 Fragments arranged by size: 6735 742 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2270 3859 2270 Size: 1589 5981 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2199 2415 2571 2863 3093 3210 3271 Size: 399 409 216 156 292 230 117 61 Cuts at: 3271 3279 3339 3426 3633 3675 3872 4005 4026 Size: 8 60 87 207 42 197 133 21 Cuts at: 4026 4146 4230 4582 4647 4665 4807 4839 4889 Size: 120 84 352 65 18 142 32 50 Cuts at: 4889 5631 5716 6787 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 409 399 352 292 230 216 207 197 156 142 133 120 117 87 85 84 65 61 60 50 42 32 21 18 8 BspEI T'CCGG_A Cuts at: 3487 7233 3487 Size: 3746 3824 BspHI T'CATG_A Cuts at: 5459 7189 5459 Size: 1730 5840 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2024 2222 2622 2651 2982 3225 4268 4925 Size: 1180 198 400 29 331 243 1043 657 Cuts at: 4925 844 Size: 3489 Fragments arranged by size: 3489 1180 1043 657 400 331 243 198 29 BsrBI CCG'CTC Cuts at: 1394 1823 3531 5457 1394 Size: 429 1708 1926 3507 Fragments arranged by size: 3507 1926 1708 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3175 3395 4627 6065 6239 65 Size: 1183 1927 220 1232 1438 174 1396 Fragments arranged by size: 1927 1438 1396 1232 1183 220 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2353 3133 3663 6214 6401 1700 Size: 110 543 780 530 2551 187 2869 Fragments arranged by size: 2869 2551 780 543 530 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7570 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7570 BssSI C'ACGA_G Cuts at: 5630 6642 5630 Size: 1012 6558 BstAPI GCAn_nnn'nTGC Cuts at: 794 2092 2791 3748 794 Size: 1298 699 957 4616 Fragments arranged by size: 4616 1298 957 699 BstBI TT'CG_AA Cuts at: 6374 6374 Size: 7570 BstEII G'GTnAC_C Cuts at: 1184 2011 3103 3160 3355 1184 Size: 827 1092 57 195 5399 Fragments arranged by size: 5399 1092 827 195 57 BstXI CCAn_nnnn'nTGG Cuts at: 2374 2549 2840 2944 3426 3865 4780 4830 2374 Size: 175 291 104 482 439 915 50 5114 Fragments arranged by size: 5114 915 482 439 291 175 104 50 BstYI r'GATC_y Cuts at: 1972 4281 4768 4900 5267 5652 5669 7121 7207 Size: 2309 487 132 367 385 17 1452 86 Cuts at: 7207 7219 7565 1972 Size: 12 346 1977 Fragments arranged by size: 2309 1977 1452 487 385 367 346 132 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4401 4401 Size: 7570 BtgI C'CryG_G Cuts at: 513 1065 2004 2712 2833 3351 3410 4839 513 Size: 552 939 708 121 518 59 1429 3244 Fragments arranged by size: 3244 1429 939 708 552 518 121 59 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2442 3697 487 Size: 683 56 1216 1255 4360 Fragments arranged by size: 4360 1255 1216 683 56 BtsI GCAGTG_nn' Cuts at: 2047 2186 2792 2831 2846 3093 3452 3992 4804 Size: 139 606 39 15 247 359 540 812 Cuts at: 4804 5086 5880 5900 2047 Size: 282 794 20 3717 Fragments arranged by size: 3717 812 794 606 540 359 282 247 139 39 20 15 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7570 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7535 DraI TTT'AAA Cuts at: 4874 4874 Size: 7570 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3287 6577 809 Size: 507 1971 3290 1802 Fragments arranged by size: 3290 1971 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2314 2355 3413 Size: 54 1425 50 275 18 484 41 1058 Cuts at: 3413 4842 5017 5906 7535 8 Size: 1429 175 889 1629 43 Fragments arranged by size: 1629 1429 1425 1058 889 484 275 175 54 50 43 41 18 EagI C'GGCC_G Cuts at: 1537 1812 2314 3413 5017 1537 Size: 275 502 1099 1604 4090 Fragments arranged by size: 4090 1604 1099 502 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3890 5500 1353 Size: 2537 1610 3423 Fragments arranged by size: 3423 2537 1610 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3886 4034 4151 6157 6531 6677 Size: 1064 138 2297 148 117 2006 374 146 Cuts at: 6677 387 Size: 1280 Fragments arranged by size: 2297 2006 1280 1064 374 148 146 138 117 EcoICRI GAG'CTC Cuts at: 3208 3208 Size: 7570 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2058 2621 2627 2750 2877 3083 3208 Size: 393 426 563 6 123 127 206 125 Cuts at: 3208 3497 3527 4136 4757 5633 6230 7017 7285 Size: 289 30 609 621 876 597 787 268 Cuts at: 7285 1239 Size: 1524 Fragments arranged by size: 1524 876 787 621 609 597 563 426 393 289 268 206 127 125 123 30 6 EcoNI CCTnn'n_nnAGG Cuts at: 3887 3887 Size: 7570 EcoO109I rG'GnC_Cy Cuts at: 2596 3058 3534 4300 4662 4835 2596 Size: 462 476 766 362 173 5331 Fragments arranged by size: 5331 766 476 462 362 173 EcoRI G'AATT_C Cuts at: 4879 5257 4879 Size: 378 7192 FseI GG_CCGG'CC Cuts at: 3667 3667 Size: 7570 FspI TGC'GCA Cuts at: 2285 6076 2285 Size: 3791 3779 HaeII r_GCGC'y Cuts at: 1678 2036 2241 3659 3788 4491 6717 1678 Size: 358 205 1418 129 703 2226 2531 Fragments arranged by size: 2531 2226 1418 703 358 205 129 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2049 2081 2516 2548 3854 Size: 32 135 32 477 32 435 32 1306 Cuts at: 3854 3886 4026 4058 4187 4219 6211 6243 6285 Size: 32 140 32 129 32 1992 32 42 Cuts at: 6285 6317 1373 Size: 32 2626 Fragments arranged by size: 2626 1992 1306 477 435 140 135 129 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2482 2557 2971 3298 4015 4914 Size: 988 281 544 75 414 327 717 899 Cuts at: 4914 5160 669 Size: 246 3079 Fragments arranged by size: 3079 988 899 717 544 414 327 281 246 75 HindIII A'AGCT_T Cuts at: 748 748 Size: 7570 HpaI GTT'AAC Cuts at: 5160 5160 Size: 7570 KasI G'GCGC_C Cuts at: 3784 3784 Size: 7570 KpnI G_GTAC'C Cuts at: 4895 4895 Size: 7570 MfeI C'AATT_G Cuts at: 5169 5169 Size: 7570 MluI A'CGCG_T Cuts at: 7446 7446 Size: 7570 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2511 3882 6684 6868 1305 Size: 1206 1371 2802 184 2007 Fragments arranged by size: 2802 2007 1371 1206 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4844 10 Size: 54 1425 343 3012 2736 Fragments arranged by size: 3012 2736 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2171 2372 3081 3225 3332 4152 Size: 648 843 162 201 709 144 107 820 Cuts at: 4152 4828 5528 5887 6046 518 Size: 676 700 359 159 2042 Fragments arranged by size: 2042 843 820 709 700 676 648 359 201 162 159 144 107 MspA1I CmG'CkG Cuts at: 1606 2821 3049 3412 3543 3601 3745 3834 4111 Size: 1215 228 363 131 58 144 89 277 Cuts at: 4111 4828 4986 5663 6811 7056 1606 Size: 717 158 677 1148 245 2120 Fragments arranged by size: 2120 1215 1148 717 677 363 277 245 228 158 144 131 89 58 NaeI GCC'GGC Cuts at: 2355 3135 3665 2355 Size: 780 530 6260 Fragments arranged by size: 6260 780 530 NarI GG'CG_CC Cuts at: 3785 3785 Size: 7570 NcoI C'CATG_G Cuts at: 513 1065 2004 2833 4839 513 Size: 552 939 829 2006 3244 Fragments arranged by size: 3244 2006 939 829 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7570 NgoMIV G'CCGG_C Cuts at: 2353 3133 3663 2353 Size: 780 530 6260 Fragments arranged by size: 6260 780 530 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7570 NotI GC'GGCC_GC Cuts at: 2314 3413 5017 2314 Size: 1099 1604 4867 Fragments arranged by size: 4867 1604 1099 NruI TCG'CGA Cuts at: 3825 3825 Size: 7570 NspI r_CATG'y Cuts at: 1132 1229 2099 2112 3145 4417 4525 4928 6473 Size: 97 870 13 1033 1272 108 403 1545 Cuts at: 6473 1132 Size: 2229 Fragments arranged by size: 2229 1545 1272 1033 870 403 108 97 13 PasI CC'CwG_GG Cuts at: 1348 2765 2854 2869 3263 1348 Size: 1417 89 15 394 5655 Fragments arranged by size: 5655 1417 394 89 15 PciI A'CATG_T Cuts at: 4413 4521 6469 4413 Size: 108 1948 5514 Fragments arranged by size: 5514 1948 108 PflMI CCAn_nnn'nTGG Cuts at: 1108 3658 1108 Size: 2550 5020 PfoI T'CCnGG_A Cuts at: 2154 2253 4565 2154 Size: 99 2312 5159 Fragments arranged by size: 5159 2312 99 PmeI GTTT'AAAC Cuts at: 4874 4874 Size: 7570 PmlI CAC'GTG Cuts at: 3079 3079 Size: 7570 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5639 5671 7152 7184 5639 Size: 32 1481 32 6025 Fragments arranged by size: 6025 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2596 3058 3534 4300 2596 Size: 462 476 766 5866 Fragments arranged by size: 5866 766 476 462 PshAI GACnn'nnGTC Cuts at: 1613 2594 1613 Size: 981 6589 PsiI TTA'TAA Cuts at: 5140 5140 Size: 7570 PspOMI G'GGCC_C Cuts at: 4661 4835 4661 Size: 174 7396 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3959 3991 3959 Size: 32 7538 PstI C_TGCA'G Cuts at: 830 2181 2826 3987 4922 830 Size: 1351 645 1161 935 3478 Fragments arranged by size: 3478 1351 1161 935 645 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7570 PvuII CAG'CTG Cuts at: 2821 3049 3601 3745 4111 4828 2821 Size: 228 552 144 366 717 5563 Fragments arranged by size: 5563 717 552 366 228 144 RsrII CG'GwC_CG Cuts at: 4087 4087 Size: 7570 SacI G_AGCT'C Cuts at: 3210 3210 Size: 7570 SacII CC_GC'GG Cuts at: 3413 3413 Size: 7570 SalI G'TCGA_C Cuts at: 1936 4013 4912 1936 Size: 2077 899 4594 Fragments arranged by size: 4594 2077 899 SbfI CC_TGCA'GG Cuts at: 4922 4922 Size: 7570 ScaI AGT'ACT Cuts at: 5818 7530 5818 Size: 1712 5858 SfcI C'TryA_G Cuts at: 826 1045 2177 2822 3907 3983 4030 4918 6053 Size: 219 1132 645 1085 76 47 888 1135 Cuts at: 6053 6734 6925 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1132 1085 888 681 645 219 191 76 47 SfoI GGC'GCC Cuts at: 3786 3786 Size: 7570 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7570 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7570 SmaI CCC'GGG Cuts at: 1872 2906 4698 4897 1872 Size: 1034 1792 199 4545 Fragments arranged by size: 4545 1792 1034 199 SmlI C'TyrA_G Cuts at: 820 1017 1943 5674 6575 6837 820 Size: 197 926 3731 901 262 1553 Fragments arranged by size: 3731 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5361 493 Size: 4868 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7570 SphI G_CATG'C Cuts at: 1132 2099 4928 1132 Size: 967 2829 3774 Fragments arranged by size: 3774 2829 967 SrfI GCCC'GGGC Cuts at: 4698 4698 Size: 7570 SspI AAT'ATT Cuts at: 5 52 5494 5 Size: 47 5442 2081 Fragments arranged by size: 5442 2081 47 StuI AGG'CCT Cuts at: 1517 4435 1517 Size: 2918 4652 StyI C'CwwG_G Cuts at: 513 1065 2004 2592 2833 3339 4839 5009 513 Size: 552 939 588 241 506 1500 170 3074 Fragments arranged by size: 3074 1500 939 588 552 506 241 170 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2846 4145 4358 5597 5614 5767 5952 Size: 1255 1266 1299 213 1239 17 153 185 Cuts at: 5952 6397 325 Size: 445 1498 Fragments arranged by size: 1498 1299 1266 1255 1239 445 213 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2474 4119 5816 Size: 274 80 33 51 1443 497 1645 1697 Cuts at: 5816 7528 96 Size: 1712 138 Fragments arranged by size: 1712 1697 1645 1443 497 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3944 3976 4526 4558 7530 7562 3944 Size: 32 550 32 2972 32 3952 Fragments arranged by size: 3952 2972 550 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4010 1316 Size: 2694 4876 XbaI T'CTAG_A Cuts at: 4906 4906 Size: 7570 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2941 3154 4780 4852 2941 Size: 213 1626 72 5659 Fragments arranged by size: 5659 1626 213 72 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7570 XmaI C'CCGG_G Cuts at: 1870 2904 4696 4895 1870 Size: 1034 1792 199 4545 Fragments arranged by size: 4545 1792 1034 199 XmnI GAAnn'nnTTC Cuts at: 5699 5699 Size: 7570 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4729 5378 276 Size: 53 83 186 936 3195 649 2468 Fragments arranged by size: 3195 2468 936 649 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FalI FspAI NsiI PacI PspXI SanDI SapI SexAI SfiI SwaI