(Circular) (Six-base) MAPSORT of: pFN21AE0391.seq Check: 1803 from: 1 to: 8296 pFN21AE0391 8296 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6106 278 Size: 53 83 186 936 4570 2468 Fragments arranged by size: 4570 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2612 5543 5575 5639 1937 Size: 675 2931 32 64 4594 Fragments arranged by size: 4594 2931 675 64 32 Acc65I G'GTAC_C Cuts at: 5617 5617 Size: 8296 AclI AA'CG_TT Cuts at: 1563 3400 5595 6423 6796 1563 Size: 1837 2195 828 373 3063 Fragments arranged by size: 3063 2195 1837 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2171 2823 3684 4184 4637 5075 5294 6359 Size: 539 652 861 500 453 438 219 1065 Cuts at: 6359 7743 8011 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1065 861 652 539 500 453 438 268 219 AfeI AGC'GCT Cuts at: 2862 2862 Size: 8296 AflII C'TTAA_G Cuts at: 820 1017 2771 5315 820 Size: 197 1754 2544 3801 Fragments arranged by size: 3801 2544 1754 197 AflIII A'CryG_T Cuts at: 1203 2343 3147 7195 8172 1203 Size: 1140 804 4048 977 1327 Fragments arranged by size: 4048 1327 1140 977 804 AgeI A'CCGG_T Cuts at: 7127 7127 Size: 8296 AhdI GACnn_n'nnGTC Cuts at: 7025 7025 Size: 8296 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8296 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2834 2981 1184 Size: 1650 147 6499 Fragments arranged by size: 6499 1650 147 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3364 3396 3364 Size: 32 8264 AlwNI CAG_nnn'CTG Cuts at: 2659 6039 7611 2659 Size: 3380 1572 3344 Fragments arranged by size: 3380 3344 1572 ApaI G_GGCC'C Cuts at: 2687 2687 Size: 8296 ApaLI G'TGCA_C Cuts at: 6353 7509 6353 Size: 1156 7140 ApoI r'AATT_y Cuts at: 1493 2095 2399 2752 3158 3585 3687 3950 4583 Size: 602 304 353 406 427 102 263 633 Cuts at: 4583 4685 4760 4798 4862 5292 5366 5513 5605 Size: 102 75 38 64 430 74 147 92 Cuts at: 5605 5831 5983 8092 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 633 602 430 427 406 353 304 263 226 152 147 102 102 92 75 74 64 38 AseI AT'TA_AT Cuts at: 160 1032 2778 6850 160 Size: 872 1746 4072 1606 Fragments arranged by size: 4072 1746 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8296 AvaI C'yCGr_G Cuts at: 1870 1943 4404 4809 5621 1870 Size: 73 2461 405 812 4545 Fragments arranged by size: 4545 2461 812 405 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2553 2586 2553 Size: 33 8263 BamHI G'GATC_C Cuts at: 2505 5626 5993 2505 Size: 3121 367 4808 Fragments arranged by size: 4808 3121 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4227 4437 5617 7072 618 Size: 325 217 627 2440 210 1180 1455 1842 Fragments arranged by size: 2440 1842 1455 1180 627 325 217 210 BanII G_rGCy'C Cuts at: 1391 2687 4816 5615 1391 Size: 1296 2129 799 4072 Fragments arranged by size: 4072 2129 1296 799 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2394 3050 4151 5529 928 Size: 971 495 656 1101 1378 3695 Fragments arranged by size: 3695 1378 1101 971 656 495 BbvCI CC'TCA_GC Cuts at: 4104 4104 Size: 8296 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6487 6521 1903 Size: 34 4550 34 3678 Fragments arranged by size: 4550 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6188 7398 6188 Size: 1210 7086 BclI T'GATC_A Cuts at: 1548 5212 1548 Size: 3664 4632 BfrBI ATG'CAT Cuts at: 5476 5476 Size: 8296 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6907 136 Size: 107 122 71 631 605 5235 1525 Fragments arranged by size: 5235 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3753 8291 3753 Size: 4538 3758 BlpI GC'TnA_GC Cuts at: 4444 5713 4444 Size: 1269 7027 Bme1580I G_kGCm'C Cuts at: 1790 2687 2877 6357 7513 1790 Size: 897 190 3480 1156 2573 Fragments arranged by size: 3480 2573 1156 897 190 BmgBI CAC'GTC Cuts at: 1318 2884 3139 1318 Size: 1566 255 6475 Fragments arranged by size: 6475 1566 255 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6027 6985 442 Size: 416 53 83 399 24 4610 958 1753 Fragments arranged by size: 4610 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4844 1056 Size: 3788 4508 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4196 4262 4323 6956 1239 Size: 2957 66 61 2633 2579 Fragments arranged by size: 2957 2633 2579 66 61 Bpu10I CC'TnA_GC Cuts at: 4104 4520 4104 Size: 416 7880 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2917 3611 5192 6421 7286 7584 2917 Size: 694 1581 1229 865 298 3629 Fragments arranged by size: 3629 1581 1229 865 694 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6959 882 Size: 617 5460 2219 Fragments arranged by size: 5460 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3150 3512 6087 493 Size: 711 1946 362 2575 2702 Fragments arranged by size: 2702 2575 1946 711 362 BsaBI GATnn'nnATC Cuts at: 8290 8290 Size: 8296 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6103 6485 7120 275 Size: 53 83 186 936 4570 382 635 1451 Fragments arranged by size: 4570 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6729 7127 7401 7548 7959 6729 Size: 398 274 147 411 7066 Fragments arranged by size: 7066 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3218 3248 1178 Size: 30 165 30 1815 30 6226 Fragments arranged by size: 6226 1815 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2103 3239 4686 5313 1186 Size: 917 1136 1447 627 4169 Fragments arranged by size: 4169 1447 1136 917 627 BseYI C'CCAG_C Cuts at: 7499 7995 7499 Size: 496 7800 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2281 2794 3503 5397 1163 Size: 573 545 513 709 1894 4062 Fragments arranged by size: 4062 1894 709 573 545 513 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5746 6507 7535 1540 Size: 275 186 3745 761 1028 2301 Fragments arranged by size: 3745 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4163 6357 6442 7513 4163 Size: 2194 85 1071 4946 Fragments arranged by size: 4946 2194 1071 85 BsmI GAATG_Cn' Cuts at: 3043 4821 5478 5807 5900 3043 Size: 1778 657 329 93 5439 Fragments arranged by size: 5439 1778 657 329 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2687 2877 4163 4816 5615 6357 6442 Size: 399 897 190 1286 653 799 742 85 Cuts at: 6442 7513 1391 Size: 1071 2174 Fragments arranged by size: 2174 1286 1071 897 799 742 653 399 190 85 BspEI T'CCGG_A Cuts at: 7959 7959 Size: 8296 BspHI T'CATG_A Cuts at: 4641 6185 7915 4641 Size: 1544 1730 5022 Fragments arranged by size: 5022 1730 1544 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3730 5035 5651 844 Size: 2886 1305 616 3489 Fragments arranged by size: 3489 2886 1305 616 BsrBI CCG'CTC Cuts at: 1394 1823 6183 1394 Size: 429 4360 3507 Fragments arranged by size: 4360 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2154 6791 6965 65 Size: 1183 906 4637 174 1396 Fragments arranged by size: 4637 1396 1183 906 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6940 7127 1700 Size: 110 5130 187 2869 Fragments arranged by size: 5130 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 5376 96 Size: 5280 3016 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8296 BssSI C'ACGA_G Cuts at: 6356 7368 6356 Size: 1012 7284 BstAPI GCAn_nnn'nTGC Cuts at: 794 4244 4466 794 Size: 3450 222 4624 Fragments arranged by size: 4624 3450 222 BstBI TT'CG_AA Cuts at: 7100 7100 Size: 8296 BstEII G'GTnAC_C Cuts at: 1184 5022 1184 Size: 3838 4458 BstYI r'GATC_y Cuts at: 1972 2254 2505 3753 4384 5527 5626 5993 6378 Size: 282 251 1248 631 1143 99 367 385 Cuts at: 6378 6395 7847 7933 7945 8291 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1248 1143 631 385 367 346 282 251 99 86 17 12 BstZ17I GTA'TAC Cuts at: 2613 5544 2613 Size: 2931 5365 BtgI C'CryG_G Cuts at: 513 1065 2877 513 Size: 552 1812 5932 Fragments arranged by size: 5932 1812 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7557 Fragments arranged by size: 7557 683 56 BtsI GCAGTG_nn' Cuts at: 2256 5082 5812 6606 6626 2256 Size: 2826 730 794 20 3926 Fragments arranged by size: 3926 2826 794 730 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8296 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5058 5093 529 Size: 35 4494 35 3732 Fragments arranged by size: 4494 3732 35 35 DraI TTT'AAA Cuts at: 4629 5600 4629 Size: 971 7325 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7303 809 Size: 507 5987 1802 Fragments arranged by size: 5987 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3859 4941 4974 Size: 54 1425 50 275 18 2029 1082 33 Cuts at: 4974 5743 6632 8261 8 Size: 769 889 1629 43 Fragments arranged by size: 2029 1629 1425 1082 889 769 275 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 5743 1537 Size: 275 3931 4090 Fragments arranged by size: 4090 3931 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2842 3455 3996 4783 5037 5142 6226 1353 Size: 1489 613 541 787 254 105 1084 3423 Fragments arranged by size: 3423 1489 1084 787 613 541 254 105 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6883 7257 7403 387 Size: 1064 138 5294 374 146 1280 Fragments arranged by size: 5294 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2171 2823 3684 4184 4196 4262 4323 Size: 393 539 652 861 500 12 66 61 Cuts at: 4323 4637 5075 5294 6359 6956 7743 8011 1239 Size: 314 438 219 1065 597 787 268 1524 Fragments arranged by size: 1524 1065 861 787 652 597 539 500 438 393 314 268 219 66 61 12 EcoO109I rG'GnC_Cy Cuts at: 2683 5008 2683 Size: 2325 5971 EcoRI G'AATT_C Cuts at: 2399 4583 5605 5983 2399 Size: 2184 1022 378 4712 Fragments arranged by size: 4712 2184 1022 378 EcoRV GAT'ATC Cuts at: 3681 3681 Size: 8296 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3105 3105 Size: 8296 FspI TGC'GCA Cuts at: 6802 6802 Size: 8296 HaeII r_GCGC'y Cuts at: 1678 2653 2864 7443 1678 Size: 975 211 4579 2531 Fragments arranged by size: 4579 2531 975 211 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2036 2042 2068 2074 3218 Size: 32 135 32 464 6 26 6 1144 Cuts at: 3218 3231 3250 3263 3464 3496 4138 4170 5204 Size: 13 19 13 201 32 642 32 1034 Cuts at: 5204 5236 5519 5551 6937 6969 7011 7043 1373 Size: 32 283 32 1386 32 42 32 2626 Fragments arranged by size: 2626 1386 1144 1034 642 464 283 201 135 42 32 32 32 32 32 32 32 32 26 19 13 13 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 4806 5485 5640 5886 669 Size: 988 281 2868 679 155 246 3079 Fragments arranged by size: 3079 2868 988 679 281 246 155 HindIII A'AGCT_T Cuts at: 748 748 Size: 8296 HpaI GTT'AAC Cuts at: 4806 5485 5886 4806 Size: 679 401 7216 Fragments arranged by size: 7216 679 401 KpnI G_GTAC'C Cuts at: 5621 5621 Size: 8296 MfeI C'AATT_G Cuts at: 3810 3855 4857 5895 3810 Size: 45 1002 1038 6211 Fragments arranged by size: 6211 1038 1002 45 MluI A'CGCG_T Cuts at: 8172 8172 Size: 8296 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2974 7410 7594 1305 Size: 1669 4436 184 2007 Fragments arranged by size: 4436 2007 1669 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3861 4943 4976 10 Size: 54 1425 343 2029 1082 33 3330 Fragments arranged by size: 3330 2029 1425 1082 343 54 33 MslI CAynn'nnrTG Cuts at: 518 1166 2166 6254 6613 6772 518 Size: 648 1000 4088 359 159 2042 Fragments arranged by size: 4088 2042 1000 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4166 4204 5712 6389 7537 7782 1606 Size: 2560 38 1508 677 1148 245 2120 Fragments arranged by size: 2560 2120 1508 1148 677 245 38 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7744 NdeI CA'TA_TG Cuts at: 387 387 Size: 8296 NheI G'CTAG_C Cuts at: 1052 4840 1052 Size: 3788 4508 NotI GC'GGCC_GC Cuts at: 5743 5743 Size: 8296 NsiI A_TGCA'T Cuts at: 5478 5478 Size: 8296 NspI r_CATG'y Cuts at: 1132 1229 2347 3709 4078 5654 7199 1132 Size: 97 1118 1362 369 1576 1545 2229 Fragments arranged by size: 2229 1576 1545 1362 1118 369 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8296 PciI A'CATG_T Cuts at: 2343 7195 2343 Size: 4852 3444 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8296 PfoI T'CCnGG_A Cuts at: 4278 4339 4278 Size: 61 8235 PmeI GTTT'AAAC Cuts at: 5600 5600 Size: 8296 PmlI CAC'GTG Cuts at: 3150 3150 Size: 8296 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2136 2168 6365 6397 7878 7910 2136 Size: 32 4197 32 1481 32 2522 Fragments arranged by size: 4197 2522 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 5008 5008 Size: 8296 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8296 PsiI TTA'TAA Cuts at: 4038 5866 4038 Size: 1828 6468 PspOMI G'GGCC_C Cuts at: 2683 2683 Size: 8296 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2217 2249 5121 5153 2217 Size: 32 2872 32 5360 Fragments arranged by size: 5360 2872 32 32 PstI C_TGCA'G Cuts at: 830 2141 3727 3844 4213 4224 4578 5648 830 Size: 1311 1586 117 369 11 354 1070 3478 Fragments arranged by size: 3478 1586 1311 1070 369 354 117 11 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8296 PvuII CAG'CTG Cuts at: 4204 4204 Size: 8296 SalI G'TCGA_C Cuts at: 1936 5638 1936 Size: 3702 4594 SapI GCTCTTCn'nnn_ Cuts at: 5037 5037 Size: 8296 SbfI CC_TGCA'GG Cuts at: 3727 5648 3727 Size: 1921 6375 ScaI AGT'ACT Cuts at: 6544 8256 6544 Size: 1712 6584 SfcI C'TryA_G Cuts at: 826 1045 2137 3217 3723 3840 4209 4220 4574 Size: 219 1092 1080 506 117 369 11 354 Cuts at: 4574 5062 5644 6779 7460 7651 826 Size: 488 582 1135 681 191 1471 Fragments arranged by size: 1471 1135 1092 1080 681 582 506 488 369 354 219 191 117 11 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8296 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8296 SmaI CCC'GGG Cuts at: 1872 5623 1872 Size: 3751 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2771 2896 3626 5171 5315 6400 Size: 197 926 828 125 730 1545 144 1085 Cuts at: 6400 7301 7563 820 Size: 901 262 1553 Fragments arranged by size: 1553 1545 1085 926 901 828 730 262 197 144 125 SnaBI TAC'GTA Cuts at: 493 6087 493 Size: 5594 2702 SpeI A'CTAG_T Cuts at: 152 2566 152 Size: 2414 5882 SphI G_CATG'C Cuts at: 1132 4078 5654 1132 Size: 2946 1576 3774 Fragments arranged by size: 3774 2946 1576 SspI AAT'ATT Cuts at: 5 52 2428 3572 4412 6220 5 Size: 47 2376 1144 840 1808 2081 Fragments arranged by size: 2376 2081 1808 1144 840 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8296 StyI C'CwwG_G Cuts at: 513 1065 5735 513 Size: 552 4670 3074 Fragments arranged by size: 4670 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2100 6323 6340 6493 6678 7123 325 Size: 1255 520 4223 17 153 185 445 1498 Fragments arranged by size: 4223 1498 1255 520 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4309 5376 6542 Size: 274 80 33 51 1443 2332 1067 1166 Cuts at: 6542 8254 96 Size: 1712 138 Fragments arranged by size: 2332 1712 1443 1166 1067 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4312 4344 4956 4988 8256 8288 4312 Size: 32 612 32 3268 32 4320 Fragments arranged by size: 4320 3268 612 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4150 1316 Size: 2834 5462 XbaI T'CTAG_A Cuts at: 3610 5632 3610 Size: 2022 6274 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3692 3692 Size: 8296 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8296 XmaI C'CCGG_G Cuts at: 1870 5621 1870 Size: 3751 4545 XmnI GAAnn'nnTTC Cuts at: 5338 6425 5338 Size: 1087 7209 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6104 276 Size: 53 83 186 936 4570 2468 Fragments arranged by size: 4570 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BbeI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI