(Circular) (Six-base) MAPSORT of: pFN21AE0390.seq Check: 9182 from: 1 to: 6169 pFN21AE0390 6169 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3231 3979 278 Size: 53 83 186 936 1695 748 2468 Fragments arranged by size: 2468 1695 936 748 186 83 53 AccI GT'mk_AC Cuts at: 1937 3369 3512 1937 Size: 1432 143 4594 Fragments arranged by size: 4594 1432 143 Acc65I G'GTAC_C Cuts at: 3330 3490 3330 Size: 160 6009 AclI AA'CG_TT Cuts at: 1563 4296 4669 1563 Size: 2733 373 3063 Fragments arranged by size: 3063 2733 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2855 4232 5616 5884 1632 Size: 1223 1377 1384 268 1917 Fragments arranged by size: 1917 1384 1377 1223 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5972 AflIII A'CryG_T Cuts at: 1203 2667 5068 6045 1203 Size: 1464 2401 977 1327 Fragments arranged by size: 2401 1464 1327 977 AgeI A'CCGG_T Cuts at: 5000 5000 Size: 6169 AhdI GACnn_n'nnGTC Cuts at: 3232 4898 3232 Size: 1666 4503 AleI CACnn'nnGTG Cuts at: 1166 2321 1166 Size: 1155 5014 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6169 AlwNI CAG_nnn'CTG Cuts at: 2238 2401 2833 3188 3912 5484 2238 Size: 163 432 355 724 1572 2923 Fragments arranged by size: 2923 1572 724 432 355 163 ApaI G_GGCC'C Cuts at: 2491 2968 2491 Size: 477 5692 ApaLI G'TGCA_C Cuts at: 3044 4226 5382 3044 Size: 1182 1156 3831 Fragments arranged by size: 3831 1182 1156 ApoI r'AATT_y Cuts at: 1493 2690 3478 3704 3856 5965 1493 Size: 1197 788 226 152 2109 1697 Fragments arranged by size: 2109 1697 1197 788 226 152 AseI AT'TA_AT Cuts at: 160 1032 4723 160 Size: 872 3691 1606 Fragments arranged by size: 3691 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6169 AvaI C'yCGr_G Cuts at: 1870 1943 2096 2462 2656 2881 2954 3148 3404 Size: 73 153 366 194 225 73 194 256 Cuts at: 3404 3494 1870 Size: 90 4545 Fragments arranged by size: 4545 366 256 225 194 194 153 90 73 73 BamHI G'GATC_C Cuts at: 3499 3866 3499 Size: 367 5802 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2090 2948 3330 3490 4945 Size: 325 217 627 303 858 382 160 1455 Cuts at: 4945 618 Size: 1842 Fragments arranged by size: 1842 1455 858 627 382 325 303 217 160 BanII G_rGCy'C Cuts at: 1391 2307 2491 2759 2968 3488 1391 Size: 916 184 268 209 520 4072 Fragments arranged by size: 4072 916 520 268 209 184 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3202 928 Size: 971 1303 3895 Fragments arranged by size: 3895 1303 971 BbvCI CC'TCA_GC Cuts at: 2163 3009 2163 Size: 846 5323 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2862 2896 4360 4394 1903 Size: 34 925 34 1464 34 3678 Fragments arranged by size: 3678 1464 925 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4061 5271 4061 Size: 1210 4959 BclI T'GATC_A Cuts at: 1548 1548 Size: 6169 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2466 3359 4780 Size: 107 122 71 631 605 794 893 1421 Cuts at: 4780 136 Size: 1525 Fragments arranged by size: 1525 1421 893 794 631 605 122 107 71 BglII A'GATC_T Cuts at: 2058 6164 2058 Size: 4106 2063 BlpI GC'TnA_GC Cuts at: 3586 3586 Size: 6169 Bme1580I G_kGCm'C Cuts at: 1790 2130 2245 2313 2491 2951 2968 3048 4230 Size: 340 115 68 178 460 17 80 1182 Cuts at: 4230 5386 1790 Size: 1156 2573 Fragments arranged by size: 2573 1182 1156 460 340 178 115 80 68 17 BmgBI CAC'GTC Cuts at: 1318 2668 1318 Size: 1350 4819 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2117 3900 4858 Size: 416 53 83 399 24 700 1783 958 Cuts at: 4858 442 Size: 1753 Fragments arranged by size: 1783 1753 958 700 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6169 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2365 2365 Size: 6169 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2594 3047 3404 3412 4829 1239 Size: 1355 453 357 8 1417 2579 Fragments arranged by size: 2579 1417 1355 453 357 8 Bpu10I CC'TnA_GC Cuts at: 2163 3009 2163 Size: 846 5323 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3113 4294 5159 5457 3113 Size: 1181 865 298 3825 Fragments arranged by size: 3825 1181 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2521 4832 882 Size: 617 1022 2311 2219 Fragments arranged by size: 2311 2219 1022 617 BsaAI yAC'GTr Cuts at: 493 1204 2323 3373 3960 493 Size: 711 1119 1050 587 2702 Fragments arranged by size: 2702 1119 1050 711 587 BsaBI GATnn'nnATC Cuts at: 6163 6163 Size: 6169 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3228 3243 3976 4358 Size: 53 83 186 936 1695 15 733 382 Cuts at: 4358 4993 275 Size: 635 1451 Fragments arranged by size: 1695 1451 936 733 635 382 186 83 53 15 BsaWI w'CCGG_w Cuts at: 4602 5000 5274 5421 5832 4602 Size: 398 274 147 411 4939 Fragments arranged by size: 4939 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2501 2531 2882 2912 3128 Size: 30 165 30 1098 30 351 30 216 Cuts at: 3128 3158 1178 Size: 30 4189 Fragments arranged by size: 4189 1098 351 216 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2396 2591 3044 3409 1186 Size: 1210 195 453 365 3946 Fragments arranged by size: 3946 1210 453 365 195 BseYI C'CCAG_C Cuts at: 2294 2447 2710 2752 5372 5868 2294 Size: 153 263 42 2620 496 2595 Fragments arranged by size: 2620 2595 496 263 153 42 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2852 3236 3428 1163 Size: 573 1116 384 192 3904 Fragments arranged by size: 3904 1116 573 384 192 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3619 4380 5408 1540 Size: 275 186 1618 761 1028 2301 Fragments arranged by size: 2301 1618 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2882 3048 3075 3378 4230 4315 5386 2882 Size: 166 27 303 852 85 1071 3665 Fragments arranged by size: 3665 1071 852 303 166 85 27 BsmI GAATG_Cn' Cuts at: 3680 3773 3680 Size: 93 6076 BsmBI CGTCTCn'nnnn_ Cuts at: 2336 3299 2336 Size: 963 5206 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2130 2245 2307 2313 2491 2759 2882 Size: 399 340 115 62 6 178 268 123 Cuts at: 2882 2951 2968 3048 3075 3378 3488 4230 4315 Size: 69 17 80 27 303 110 742 85 Cuts at: 4315 5386 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 340 303 268 178 123 115 110 85 80 69 62 27 17 6 BspEI T'CCGG_A Cuts at: 5832 5832 Size: 6169 BspHI T'CATG_A Cuts at: 4058 5788 4058 Size: 1730 4439 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2553 2940 3524 844 Size: 1709 387 584 3489 Fragments arranged by size: 3489 1709 584 387 BsrBI CCG'CTC Cuts at: 1394 1823 2677 2854 4056 1394 Size: 429 854 177 1202 3507 Fragments arranged by size: 3507 1202 854 429 177 BsrDI GCAATG_nn' Cuts at: 65 1248 4664 4838 65 Size: 1183 3416 174 1396 Fragments arranged by size: 3416 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2065 3333 4813 5000 1700 Size: 110 255 1268 1480 187 2869 Fragments arranged by size: 2869 1480 1268 255 187 110 BsrGI T'GTAC_A Cuts at: 96 2898 96 Size: 2802 3367 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6169 BssSI C'ACGA_G Cuts at: 2306 2849 3074 3254 3302 4229 5241 2306 Size: 543 225 180 48 927 1012 3234 Fragments arranged by size: 3234 1012 927 543 225 180 48 BstAPI GCAn_nnn'nTGC Cuts at: 794 3322 794 Size: 2528 3641 BstBI TT'CG_AA Cuts at: 4973 4973 Size: 6169 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6169 BstYI r'GATC_y Cuts at: 1972 2058 2823 3165 3499 3866 4251 4268 5720 Size: 86 765 342 334 367 385 17 1452 Cuts at: 5720 5806 5818 6164 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 765 385 367 346 342 334 86 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2018 3189 2018 Size: 1171 4998 BtgI C'CryG_G Cuts at: 513 1065 2004 2086 2224 2520 2766 513 Size: 552 939 82 138 296 246 3916 Fragments arranged by size: 3916 939 552 296 246 138 82 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5430 Fragments arranged by size: 5430 683 56 BtsI GCAGTG_nn' Cuts at: 2880 3685 4479 4499 2880 Size: 805 794 20 4550 Fragments arranged by size: 4550 805 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6169 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6134 DraI TTT'AAA Cuts at: 3473 3473 Size: 6169 DraIII CAC_nnn'GTG Cuts at: 2875 2875 Size: 6169 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5176 809 Size: 507 3860 1802 Fragments arranged by size: 3860 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2063 2325 2678 Size: 54 1425 50 275 18 233 262 353 Cuts at: 2678 3057 3195 3271 3616 4505 6134 8 Size: 379 138 76 345 889 1629 43 Fragments arranged by size: 1629 1425 889 379 353 345 275 262 233 138 76 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3616 1537 Size: 275 1804 4090 Fragments arranged by size: 4090 1804 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2174 2244 4099 1353 Size: 821 70 1855 3423 Fragments arranged by size: 3423 1855 821 70 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2210 3377 4756 5130 5276 387 Size: 1064 138 621 1167 1379 374 146 1280 Fragments arranged by size: 1379 1280 1167 1064 621 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2594 2855 3047 3404 3412 4232 4829 Size: 393 962 261 192 357 8 820 597 Cuts at: 4829 5616 5884 1239 Size: 787 268 1524 Fragments arranged by size: 1524 962 820 787 597 393 357 268 261 192 8 EcoNI CCTnn'n_nnAGG Cuts at: 2249 2549 2816 2249 Size: 300 267 5602 Fragments arranged by size: 5602 300 267 EcoO109I rG'GnC_Cy Cuts at: 2100 2430 2653 2839 2965 2100 Size: 330 223 186 126 5304 Fragments arranged by size: 5304 330 223 186 126 EcoRI G'AATT_C Cuts at: 2690 3478 3856 2690 Size: 788 378 5003 Fragments arranged by size: 5003 788 378 FspI TGC'GCA Cuts at: 2921 4675 2921 Size: 1754 4415 HaeII r_GCGC'y Cuts at: 1678 2301 3188 5316 1678 Size: 623 887 2128 2531 Fragments arranged by size: 2531 2128 887 623 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3128 3160 3218 3250 4810 Size: 32 135 32 1556 32 58 32 1560 Cuts at: 4810 4842 4884 4916 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1560 1556 135 58 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3513 3759 669 Size: 988 281 1575 246 3079 Fragments arranged by size: 3079 1575 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6169 HpaI GTT'AAC Cuts at: 3759 3759 Size: 6169 KpnI G_GTAC'C Cuts at: 3334 3494 3334 Size: 160 6009 MfeI C'AATT_G Cuts at: 3768 3768 Size: 6169 MluI A'CGCG_T Cuts at: 6045 6045 Size: 6169 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2363 5283 5467 1305 Size: 1058 2920 184 2007 Fragments arranged by size: 2920 2007 1058 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2327 3273 10 Size: 54 1425 343 495 946 2906 Fragments arranged by size: 2906 1425 946 495 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2321 4127 4486 4645 518 Size: 648 1155 1806 359 159 2042 Fragments arranged by size: 2042 1806 1155 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2226 2401 2528 3585 4262 5410 5655 1606 Size: 620 175 127 1057 677 1148 245 2120 Fragments arranged by size: 2120 1148 1057 677 620 245 175 127 NaeI GCC'GGC Cuts at: 2067 2067 Size: 6169 NcoI C'CATG_G Cuts at: 513 1065 2004 2086 2520 513 Size: 552 939 82 434 4162 Fragments arranged by size: 4162 939 552 434 82 NdeI CA'TA_TG Cuts at: 387 387 Size: 6169 NgoMIV G'CCGG_C Cuts at: 2065 2065 Size: 6169 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6169 NotI GC'GGCC_GC Cuts at: 3616 3616 Size: 6169 NspI r_CATG'y Cuts at: 1132 1229 2644 3527 5072 1132 Size: 97 1415 883 1545 2229 Fragments arranged by size: 2229 1545 1415 883 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6169 PciI A'CATG_T Cuts at: 5068 5068 Size: 6169 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6169 PmeI GTTT'AAAC Cuts at: 3473 3473 Size: 6169 PmlI CAC'GTG Cuts at: 2323 2323 Size: 6169 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4238 4270 5751 5783 4238 Size: 32 1481 32 4624 Fragments arranged by size: 4624 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2100 2100 Size: 6169 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6169 PsiI TTA'TAA Cuts at: 3739 3739 Size: 6169 PspOMI G'GGCC_C Cuts at: 2487 2964 2487 Size: 477 5692 PstI C_TGCA'G Cuts at: 830 2550 2634 2937 3315 3521 830 Size: 1720 84 303 378 206 3478 Fragments arranged by size: 3478 1720 378 303 206 84 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6169 PvuII CAG'CTG Cuts at: 2401 2528 2401 Size: 127 6042 SacII CC_GC'GG Cuts at: 2227 2227 Size: 6169 SalI G'TCGA_C Cuts at: 1936 3511 1936 Size: 1575 4594 SanDI GG'GwC_CC Cuts at: 2100 2100 Size: 6169 SapI GCTCTTCn'nnn_ Cuts at: 2174 2174 Size: 6169 SbfI CC_TGCA'GG Cuts at: 2937 3521 2937 Size: 584 5585 ScaI AGT'ACT Cuts at: 4417 6129 4417 Size: 1712 4457 SfcI C'TryA_G Cuts at: 826 1045 2546 2630 2933 3311 3517 4652 5333 Size: 219 1501 84 303 378 206 1135 681 Cuts at: 5333 5524 826 Size: 191 1471 Fragments arranged by size: 1501 1471 1135 681 378 303 219 206 191 84 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6169 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6169 SmaI CCC'GGG Cuts at: 1872 2098 3496 1872 Size: 226 1398 4545 Fragments arranged by size: 4545 1398 226 SmlI C'TyrA_G Cuts at: 820 1017 1943 3128 4273 5174 5436 820 Size: 197 926 1185 1145 901 262 1553 Fragments arranged by size: 1553 1185 1145 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3960 493 Size: 3467 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6169 SphI G_CATG'C Cuts at: 1132 2644 3527 1132 Size: 1512 883 3774 Fragments arranged by size: 3774 1512 883 SspI AAT'ATT Cuts at: 5 52 4093 5 Size: 47 4041 2081 Fragments arranged by size: 4041 2081 47 StuI AGG'CCT Cuts at: 1517 2745 2939 1517 Size: 1228 194 4747 Fragments arranged by size: 4747 1228 194 StyI C'CwwG_G Cuts at: 513 1065 2004 2086 2328 2496 2520 2698 2817 Size: 552 939 82 242 168 24 178 119 Cuts at: 2817 3608 513 Size: 791 3074 Fragments arranged by size: 3074 939 791 552 242 178 168 119 82 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2333 2431 2702 4196 4213 4366 4551 Size: 1255 753 98 271 1494 17 153 185 Cuts at: 4551 4996 325 Size: 445 1498 Fragments arranged by size: 1498 1494 1255 753 445 271 185 153 98 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2898 4415 6127 Size: 274 80 33 51 1443 921 1517 1712 Cuts at: 6127 96 Size: 138 Fragments arranged by size: 1712 1517 1443 921 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2466 2498 2698 2730 2732 2764 6129 6161 2466 Size: 32 200 32 2 32 3365 32 2474 Fragments arranged by size: 3365 2474 200 32 32 32 32 2 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6169 XbaI T'CTAG_A Cuts at: 3505 3505 Size: 6169 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2320 2320 Size: 6169 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6169 XmaI C'CCGG_G Cuts at: 1870 2096 3494 1870 Size: 226 1398 4545 Fragments arranged by size: 4545 1398 226 XmnI GAAnn'nnTTC Cuts at: 4298 4298 Size: 6169 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3229 3977 276 Size: 53 83 186 936 1695 748 2468 Fragments arranged by size: 2468 1695 936 748 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SexAI SfiI SfoI SrfI SwaI