(Circular) (Six-base) MAPSORT of: pFN21AE0389.seq Check: 7620 from: 1 to: 7213 pFN21AE0389 7213 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2790 3327 2790 Size: 537 6676 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5023 278 Size: 53 83 186 936 3487 2468 Fragments arranged by size: 3487 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3675 4556 1937 Size: 1738 881 4594 Fragments arranged by size: 4594 1738 881 Acc65I G'GTAC_C Cuts at: 4534 4534 Size: 7213 AclI AA'CG_TT Cuts at: 1563 5340 5713 1563 Size: 3777 373 3063 Fragments arranged by size: 3777 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2321 2614 3427 3844 5276 6660 6928 1632 Size: 689 293 813 417 1432 1384 268 1917 Fragments arranged by size: 1917 1432 1384 813 689 417 293 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7016 AflIII A'CryG_T Cuts at: 1203 3218 6112 7089 1203 Size: 2015 2894 977 1327 Fragments arranged by size: 2894 2015 1327 977 AgeI A'CCGG_T Cuts at: 3264 6044 3264 Size: 2780 4433 AhdI GACnn_n'nnGTC Cuts at: 2302 5942 2302 Size: 3640 3573 AleI CACnn'nnGTG Cuts at: 1166 3223 3722 1166 Size: 2057 499 4657 Fragments arranged by size: 4657 2057 499 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7213 AlwNI CAG_nnn'CTG Cuts at: 3403 3481 3601 3912 4956 6528 3403 Size: 78 120 311 1044 1572 4088 Fragments arranged by size: 4088 1572 1044 311 120 78 ApaI G_GGCC'C Cuts at: 4109 4354 4109 Size: 245 6968 ApaLI G'TGCA_C Cuts at: 5270 6426 5270 Size: 1156 6057 ApoI r'AATT_y Cuts at: 1493 4522 4748 4900 7009 1493 Size: 3029 226 152 2109 1697 Fragments arranged by size: 3029 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 2066 2066 Size: 7213 AseI AT'TA_AT Cuts at: 160 1032 5767 160 Size: 872 4735 1606 Fragments arranged by size: 4735 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7213 AvaI C'yCGr_G Cuts at: 1870 1943 2860 2879 3502 3843 4055 4538 1870 Size: 73 917 19 623 341 212 483 4545 Fragments arranged by size: 4545 917 623 483 341 212 73 19 BamHI G'GATC_C Cuts at: 2976 4543 4910 2976 Size: 1567 367 5279 Fragments arranged by size: 5279 1567 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3298 3358 3388 4304 4534 Size: 325 217 627 1511 60 30 916 230 Cuts at: 4534 5989 618 Size: 1455 1842 Fragments arranged by size: 1842 1511 1455 916 627 325 230 217 60 30 BanII G_rGCy'C Cuts at: 1391 2751 2867 4062 4109 4354 4497 4532 1391 Size: 1360 116 1195 47 245 143 35 4072 Fragments arranged by size: 4072 1360 1195 245 143 116 47 35 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4100 928 Size: 971 2201 4041 Fragments arranged by size: 4041 2201 971 BbvCI CC'TCA_GC Cuts at: 3849 3849 Size: 7213 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5404 5438 1903 Size: 34 3467 34 3678 Fragments arranged by size: 3678 3467 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5105 6315 5105 Size: 1210 6003 BclI T'GATC_A Cuts at: 1548 3822 1548 Size: 2274 4939 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3507 5824 136 Size: 107 122 71 631 605 1835 2317 1525 Fragments arranged by size: 2317 1835 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3855 7208 3855 Size: 3353 3860 BlpI GC'TnA_GC Cuts at: 2698 3374 4630 2698 Size: 676 1256 5281 Fragments arranged by size: 5281 1256 676 Bme1580I G_kGCm'C Cuts at: 1790 3303 3363 3747 4109 4307 4354 5274 6430 Size: 1513 60 384 362 198 47 920 1156 Cuts at: 6430 1790 Size: 2573 Fragments arranged by size: 2573 1513 1156 920 384 362 198 60 47 BmgBI CAC'GTC Cuts at: 1318 2757 3221 1318 Size: 1439 464 5310 Fragments arranged by size: 5310 1439 464 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3297 3703 4944 Size: 416 53 83 399 24 1880 406 1241 Cuts at: 4944 5902 442 Size: 958 1753 Fragments arranged by size: 1880 1753 1241 958 416 406 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7213 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2241 2915 3278 3574 4330 5873 1239 Size: 1002 674 363 296 756 1543 2579 Fragments arranged by size: 2579 1543 1002 756 674 363 296 Bpu10I CC'TnA_GC Cuts at: 3849 3849 Size: 7213 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3070 5338 6203 6501 3070 Size: 2268 865 298 3782 Fragments arranged by size: 3782 2268 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2032 3744 5876 882 Size: 617 533 1712 2132 2219 Fragments arranged by size: 2219 2132 1712 617 533 BsaAI yAC'GTr Cuts at: 493 1204 3040 5004 493 Size: 711 1836 1964 2702 Fragments arranged by size: 2702 1964 1836 711 BsaBI GATnn'nnATC Cuts at: 7207 7207 Size: 7213 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5020 5402 6037 275 Size: 53 83 186 936 3487 382 635 1451 Fragments arranged by size: 3487 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3264 5646 6044 6318 6465 6876 3264 Size: 2382 398 274 147 411 3601 Fragments arranged by size: 3601 2382 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3179 3209 3476 3506 4020 Size: 30 165 30 1776 30 267 30 514 Cuts at: 4020 4050 4178 4208 4412 4442 1178 Size: 30 128 30 204 30 3949 Fragments arranged by size: 3949 1776 514 267 204 165 128 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2852 3866 4377 4391 1186 Size: 1666 1014 511 14 4008 Fragments arranged by size: 4008 1666 1014 511 14 BseYI C'CCAG_C Cuts at: 2056 2140 3470 3569 6416 6912 2056 Size: 84 1330 99 2847 496 2357 Fragments arranged by size: 2847 2357 1330 496 99 84 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2760 3238 1163 Size: 573 1024 478 5138 Fragments arranged by size: 5138 1024 573 478 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2500 4295 4663 5424 6452 1540 Size: 275 186 499 1795 368 761 1028 2301 Fragments arranged by size: 2301 1795 1028 761 499 368 275 186 BsiHKAI G_wGCw'C Cuts at: 2751 3882 5274 5359 6430 2751 Size: 1131 1392 85 1071 3534 Fragments arranged by size: 3534 1392 1131 1071 85 BsmI GAATG_Cn' Cuts at: 3815 4724 4817 3815 Size: 909 93 6211 Fragments arranged by size: 6211 909 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2800 2800 Size: 7213 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2751 2867 3303 3363 3747 3882 4062 Size: 399 961 116 436 60 384 135 180 Cuts at: 4062 4109 4307 4354 4497 4532 5274 5359 6430 Size: 47 198 47 143 35 742 85 1071 Cuts at: 6430 1391 Size: 2174 Fragments arranged by size: 2174 1071 961 742 436 399 384 198 180 143 135 116 85 60 47 47 35 BspEI T'CCGG_A Cuts at: 6876 6876 Size: 7213 BspHI T'CATG_A Cuts at: 2751 3819 5102 6832 2751 Size: 1068 1283 1730 3132 Fragments arranged by size: 3132 1730 1283 1068 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2790 3158 3327 3969 4568 844 Size: 1946 368 169 642 599 3489 Fragments arranged by size: 3489 1946 642 599 368 169 BsrBI CCG'CTC Cuts at: 1394 1823 2646 2842 3784 3789 3945 5100 1394 Size: 429 823 196 942 5 156 1155 3507 Fragments arranged by size: 3507 1155 942 823 429 196 156 5 BsrDI GCAATG_nn' Cuts at: 65 1248 2548 2634 5708 5882 65 Size: 1183 1300 86 3074 174 1396 Fragments arranged by size: 3074 1396 1300 1183 174 86 BsrFI r'CCGG_y Cuts at: 1700 1810 2009 3264 4290 5857 6044 1700 Size: 110 199 1255 1026 1567 187 2869 Fragments arranged by size: 2869 1567 1255 1026 199 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7213 BssHII G'CGCG_C Cuts at: 1925 2066 3811 1925 Size: 141 1745 5327 Fragments arranged by size: 5327 1745 141 BssSI C'ACGA_G Cuts at: 2109 3419 3452 3940 5273 6285 2109 Size: 1310 33 488 1333 1012 3037 Fragments arranged by size: 3037 1333 1310 1012 488 33 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7213 BstBI TT'CG_AA Cuts at: 6017 6017 Size: 7213 BstEII G'GTnAC_C Cuts at: 1184 3547 3649 1184 Size: 2363 102 4748 Fragments arranged by size: 4748 2363 102 BstYI r'GATC_y Cuts at: 1972 2280 2976 3522 3855 4543 4910 5295 5312 Size: 308 696 546 333 688 367 385 17 Cuts at: 5312 6764 6850 6862 7208 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 696 688 546 385 367 346 333 308 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2441 2739 3680 2441 Size: 298 941 5974 Fragments arranged by size: 5974 941 298 BtgI C'CryG_G Cuts at: 513 1065 2004 2514 2600 3086 4224 4308 4497 Size: 552 939 510 86 486 1138 84 189 Cuts at: 4497 513 Size: 3229 Fragments arranged by size: 3229 1138 939 552 510 486 189 86 84 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4000 487 Size: 683 56 2774 3700 Fragments arranged by size: 3700 2774 683 56 BtsI GCAGTG_nn' Cuts at: 2036 4729 5523 5543 2036 Size: 2693 794 20 3706 Fragments arranged by size: 3706 2693 794 20 ClaI AT'CG_AT Cuts at: 1554 2505 1554 Size: 951 6262 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3975 4010 529 Size: 35 3411 35 3732 Fragments arranged by size: 3732 3411 35 35 DraI TTT'AAA Cuts at: 4517 4517 Size: 7213 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4004 6220 809 Size: 507 2688 2216 1802 Fragments arranged by size: 2688 2216 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2497 3456 Size: 54 1425 50 275 18 177 490 959 Cuts at: 3456 4292 4368 4660 5549 7178 8 Size: 836 76 292 889 1629 43 Fragments arranged by size: 1629 1425 959 889 836 490 292 275 177 76 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2497 4292 4660 1537 Size: 275 685 1795 368 4090 Fragments arranged by size: 4090 1795 685 368 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3971 5143 1353 Size: 2618 1172 3423 Fragments arranged by size: 3423 2618 1172 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5800 6174 6320 387 Size: 1064 138 4211 374 146 1280 Fragments arranged by size: 4211 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2749 2749 Size: 7213 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2241 2321 2614 2915 3278 3427 3574 Size: 393 609 80 293 301 363 149 147 Cuts at: 3574 3844 4330 5276 5873 6660 6928 1239 Size: 270 486 946 597 787 268 1524 Fragments arranged by size: 1524 946 787 609 597 486 393 363 301 293 270 268 149 147 80 EcoNI CCTnn'n_nnAGG Cuts at: 3869 3869 Size: 7213 EcoO109I rG'GnC_Cy Cuts at: 2145 3478 3684 3703 4105 4106 4350 4351 2145 Size: 1333 206 19 402 1 244 1 5007 Fragments arranged by size: 5007 1333 402 244 206 19 1 1 EcoRI G'AATT_C Cuts at: 4522 4900 4522 Size: 378 6835 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3910 3910 Size: 7213 FseI GG_CCGG'CC Cuts at: 2013 2013 Size: 7213 FspI TGC'GCA Cuts at: 5719 5719 Size: 7213 HaeII r_GCGC'y Cuts at: 1678 6360 1678 Size: 4682 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2137 2169 2288 2320 3498 Size: 32 135 32 565 32 119 32 1178 Cuts at: 3498 3530 4020 4052 4178 4210 5854 5886 5928 Size: 32 490 32 126 32 1644 32 42 Cuts at: 5928 5960 1373 Size: 32 2626 Fragments arranged by size: 2626 1644 1178 565 490 135 126 119 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3046 3919 4557 4803 669 Size: 988 281 1108 873 638 246 3079 Fragments arranged by size: 3079 1108 988 873 638 281 246 HindIII A'AGCT_T Cuts at: 748 2088 3892 748 Size: 1340 1804 4069 Fragments arranged by size: 4069 1804 1340 HpaI GTT'AAC Cuts at: 4803 4803 Size: 7213 KpnI G_GTAC'C Cuts at: 4538 4538 Size: 7213 MfeI C'AATT_G Cuts at: 4812 4812 Size: 7213 MluI A'CGCG_T Cuts at: 7089 7089 Size: 7213 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2844 3031 3601 6327 6511 1305 Size: 1539 187 570 2726 184 2007 Fragments arranged by size: 2726 2007 1539 570 187 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3458 4370 10 Size: 54 1425 343 1626 912 2853 Fragments arranged by size: 2853 1626 1425 912 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3223 3545 3722 3740 4203 5171 5530 Size: 648 2057 322 177 18 463 968 359 Cuts at: 5530 5689 518 Size: 159 2042 Fragments arranged by size: 2057 2042 968 648 463 359 322 177 159 18 MspA1I CmG'CkG Cuts at: 1606 2496 3838 4022 4112 4257 4629 5306 6454 Size: 890 1342 184 90 145 372 677 1148 Cuts at: 6454 6699 1606 Size: 245 2120 Fragments arranged by size: 2120 1342 1148 890 677 372 245 184 145 90 NaeI GCC'GGC Cuts at: 2011 2011 Size: 7213 NcoI C'CATG_G Cuts at: 513 1065 2004 2600 4224 4308 4497 513 Size: 552 939 596 1624 84 189 3229 Fragments arranged by size: 3229 1624 939 596 552 189 84 NdeI CA'TA_TG Cuts at: 387 4340 387 Size: 3953 3260 NgoMIV G'CCGG_C Cuts at: 2009 2009 Size: 7213 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7213 NotI GC'GGCC_GC Cuts at: 4660 4660 Size: 7213 NspI r_CATG'y Cuts at: 1132 1229 4571 6116 1132 Size: 97 3342 1545 2229 Fragments arranged by size: 3342 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7213 PciI A'CATG_T Cuts at: 6112 6112 Size: 7213 PflMI CCAn_nnn'nTGG Cuts at: 1108 3001 1108 Size: 1893 5320 PfoI T'CCnGG_A Cuts at: 2555 3344 3728 3828 2555 Size: 789 384 100 5940 Fragments arranged by size: 5940 789 384 100 PmeI GTTT'AAAC Cuts at: 4517 4517 Size: 7213 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5282 5314 6795 6827 5282 Size: 32 1481 32 5668 Fragments arranged by size: 5668 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2145 3478 3684 2145 Size: 1333 206 5674 Fragments arranged by size: 5674 1333 206 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7213 PsiI TTA'TAA Cuts at: 4783 4783 Size: 7213 PspOMI G'GGCC_C Cuts at: 4105 4350 4105 Size: 245 6968 PstI C_TGCA'G Cuts at: 830 2424 2540 2991 3155 3408 3935 4272 4565 Size: 1594 116 451 164 253 527 337 293 Cuts at: 4565 830 Size: 3478 Fragments arranged by size: 3478 1594 527 451 337 293 253 164 116 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7213 RsrII CG'GwC_CG Cuts at: 2453 2453 Size: 7213 SacI G_AGCT'C Cuts at: 2751 2751 Size: 7213 SalI G'TCGA_C Cuts at: 1936 4555 1936 Size: 2619 4594 SanDI GG'GwC_CC Cuts at: 2145 2145 Size: 7213 SapI GCTCTTCn'nnn_ Cuts at: 3971 3971 Size: 7213 SbfI CC_TGCA'GG Cuts at: 4565 4565 Size: 7213 ScaI AGT'ACT Cuts at: 5461 7173 5461 Size: 1712 5501 SexAI A'CCwGG_T Cuts at: 3753 3753 Size: 7213 SfcI C'TryA_G Cuts at: 826 1045 2420 2536 2987 3151 3404 3931 4244 Size: 219 1375 116 451 164 253 527 313 Cuts at: 4244 4268 4561 5696 6377 6568 826 Size: 24 293 1135 681 191 1471 Fragments arranged by size: 1471 1375 1135 681 527 451 313 293 253 219 191 164 116 24 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7213 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7213 SmaI CCC'GGG Cuts at: 1872 2862 3504 3845 4540 1872 Size: 990 642 341 695 4545 Fragments arranged by size: 4545 990 695 642 341 SmlI C'TyrA_G Cuts at: 820 1017 1943 3049 5317 6218 6480 820 Size: 197 926 1106 2268 901 262 1553 Fragments arranged by size: 2268 1553 1106 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5004 493 Size: 4511 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7213 SphI G_CATG'C Cuts at: 1132 4571 1132 Size: 3439 3774 SspI AAT'ATT Cuts at: 5 52 2293 5137 5 Size: 47 2241 2844 2081 Fragments arranged by size: 2844 2241 2081 47 StuI AGG'CCT Cuts at: 1517 3587 1517 Size: 2070 5143 StyI C'CwwG_G Cuts at: 513 1065 2004 2600 3363 3986 4224 4308 4497 Size: 552 939 596 763 623 238 84 189 Cuts at: 4497 4652 513 Size: 155 3074 Fragments arranged by size: 3074 939 763 623 596 552 238 189 155 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2048 2441 4348 5240 5257 5410 5595 Size: 1255 468 393 1907 892 17 153 185 Cuts at: 5595 6040 325 Size: 445 1498 Fragments arranged by size: 1907 1498 1255 892 468 445 393 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3483 5459 7171 Size: 274 80 33 51 1443 1506 1976 1712 Cuts at: 7171 96 Size: 138 Fragments arranged by size: 1976 1712 1506 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3179 3211 4456 4488 7173 7205 3179 Size: 32 1245 32 2685 32 3187 Fragments arranged by size: 3187 2685 1245 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2477 3607 1316 Size: 1161 1130 4922 Fragments arranged by size: 4922 1161 1130 XbaI T'CTAG_A Cuts at: 3136 4549 3136 Size: 1413 5800 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3754 4439 3754 Size: 685 6528 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7213 XmaI C'CCGG_G Cuts at: 1870 2860 3502 3843 4538 1870 Size: 990 642 341 695 4545 Fragments arranged by size: 4545 990 695 642 341 XmnI GAAnn'nnTTC Cuts at: 5342 5342 Size: 7213 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5021 276 Size: 53 83 186 936 3487 2468 Fragments arranged by size: 3487 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoRV FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI PvuII SacII SfiI SfoI SrfI SwaI