(Circular) (Six-base) MAPSORT of: pFN21AE0388.seq Check: 8468 from: 1 to: 6535 pFN21AE0388 6535 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4345 278 Size: 53 83 186 936 2809 2468 Fragments arranged by size: 2809 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3878 1937 Size: 1941 4594 Acc65I G'GTAC_C Cuts at: 3856 3856 Size: 6535 AclI AA'CG_TT Cuts at: 1563 4662 5035 1563 Size: 3099 373 3063 Fragments arranged by size: 3099 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2520 2598 4598 5982 6250 1632 Size: 888 78 2000 1384 268 1917 Fragments arranged by size: 2000 1917 1384 888 268 78 AfeI AGC'GCT Cuts at: 3577 3577 Size: 6535 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6338 AflIII A'CryG_T Cuts at: 1203 2681 5434 6411 1203 Size: 1478 2753 977 1327 Fragments arranged by size: 2753 1478 1327 977 AgeI A'CCGG_T Cuts at: 5366 5366 Size: 6535 AhdI GACnn_n'nnGTC Cuts at: 5264 5264 Size: 6535 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6535 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6535 AlwNI CAG_nnn'CTG Cuts at: 2854 3423 3787 4278 5850 2854 Size: 569 364 491 1572 3539 Fragments arranged by size: 3539 1572 569 491 364 ApaI G_GGCC'C Cuts at: 2059 2074 2190 2059 Size: 15 116 6404 Fragments arranged by size: 6404 116 15 ApaLI G'TGCA_C Cuts at: 4592 5748 4592 Size: 1156 5379 ApoI r'AATT_y Cuts at: 1493 2323 2536 2548 2563 3129 3844 4070 4222 Size: 830 213 12 15 566 715 226 152 Cuts at: 4222 6331 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 830 715 566 226 213 152 15 12 AseI AT'TA_AT Cuts at: 160 1032 5089 160 Size: 872 4057 1606 Fragments arranged by size: 4057 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6535 AvaI C'yCGr_G Cuts at: 1870 1943 2050 3860 1870 Size: 73 107 1810 4545 Fragments arranged by size: 4545 1810 107 73 BamHI G'GATC_C Cuts at: 3865 4232 3865 Size: 367 6168 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3856 5311 618 Size: 325 217 627 2069 1455 1842 Fragments arranged by size: 2069 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2059 2074 2190 3854 1391 Size: 668 15 116 1664 4072 Fragments arranged by size: 4072 1664 668 116 15 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3167 928 Size: 971 1268 4296 Fragments arranged by size: 4296 1268 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4726 4760 1903 Size: 34 50 34 2705 34 3678 Fragments arranged by size: 3678 2705 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4427 5637 4427 Size: 1210 5325 BclI T'GATC_A Cuts at: 1548 1548 Size: 6535 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3824 5146 136 Size: 107 122 71 631 605 2152 1322 1525 Fragments arranged by size: 2152 1525 1322 631 605 122 107 71 BglII A'GATC_T Cuts at: 6530 6530 Size: 6535 BlpI GC'TnA_GC Cuts at: 3952 3952 Size: 6535 Bme1580I G_kGCm'C Cuts at: 1790 2059 2074 2190 4596 5752 1790 Size: 269 15 116 2406 1156 2573 Fragments arranged by size: 2573 2406 1156 269 116 15 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6535 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2307 4266 5224 Size: 416 53 83 399 24 890 1959 958 Cuts at: 5224 442 Size: 1753 Fragments arranged by size: 1959 1753 958 890 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6535 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3021 3021 Size: 6535 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3457 5195 1239 Size: 2218 1738 2579 Fragments arranged by size: 2579 2218 1738 Bpu10I CC'TnA_GC Cuts at: 2207 2207 Size: 6535 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2956 4660 5525 5823 2956 Size: 1704 865 298 3668 Fragments arranged by size: 3668 1704 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5198 882 Size: 617 3699 2219 Fragments arranged by size: 3699 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3643 4326 493 Size: 711 2439 683 2702 Fragments arranged by size: 2702 2439 711 683 BsaBI GATnn'nnATC Cuts at: 6529 6529 Size: 6535 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4342 4724 5359 275 Size: 53 83 186 936 2809 382 635 1451 Fragments arranged by size: 2809 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4968 5366 5640 5787 6198 4968 Size: 398 274 147 411 5305 Fragments arranged by size: 5305 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2156 2186 1178 Size: 30 165 30 753 30 5527 Fragments arranged by size: 5527 753 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3779 1186 Size: 2593 3942 BseYI C'CCAG_C Cuts at: 2023 2189 2371 3699 3747 5738 6234 2023 Size: 166 182 1328 48 1991 496 2324 Fragments arranged by size: 2324 1991 1328 496 182 166 48 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2315 2736 1163 Size: 573 579 421 4962 Fragments arranged by size: 4962 579 573 421 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2033 3985 4746 5774 1540 Size: 275 186 32 1952 761 1028 2301 Fragments arranged by size: 2301 1952 1028 761 275 186 32 BsiHKAI G_wGCw'C Cuts at: 4596 4681 5752 4596 Size: 85 1071 5379 Fragments arranged by size: 5379 1071 85 BsmI GAATG_Cn' Cuts at: 4046 4139 4046 Size: 93 6442 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2059 2074 2190 3854 4596 4681 5752 Size: 399 269 15 116 1664 742 85 1071 Cuts at: 5752 1391 Size: 2174 Fragments arranged by size: 2174 1664 1071 742 399 269 116 85 15 BspEI T'CCGG_A Cuts at: 6198 6198 Size: 6535 BspHI T'CATG_A Cuts at: 3618 4424 6154 3618 Size: 806 1730 3999 Fragments arranged by size: 3999 1730 806 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3890 844 Size: 3046 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4422 1394 Size: 429 2599 3507 Fragments arranged by size: 3507 2599 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2406 5030 5204 65 Size: 1183 1158 2624 174 1396 Fragments arranged by size: 2624 1396 1183 1158 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2226 5179 5366 1700 Size: 110 416 2953 187 2869 Fragments arranged by size: 2953 2869 416 187 110 BsrGI T'GTAC_A Cuts at: 96 2656 96 Size: 2560 3975 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6535 BssSI C'ACGA_G Cuts at: 4595 5607 4595 Size: 1012 5523 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6535 BstBI TT'CG_AA Cuts at: 5339 5339 Size: 6535 BstEII G'GTnAC_C Cuts at: 1184 3686 1184 Size: 2502 4033 BstXI CCAn_nnnn'nTGG Cuts at: 3534 3534 Size: 6535 BstYI r'GATC_y Cuts at: 1972 3073 3757 3865 4232 4617 4634 6086 6172 Size: 1101 684 108 367 385 17 1452 86 Cuts at: 6172 6184 6530 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1101 684 385 367 346 108 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2066 2899 2066 Size: 833 5702 BtgI C'CryG_G Cuts at: 513 1065 2059 2153 2181 3049 3234 3489 513 Size: 552 994 94 28 868 185 255 3559 Fragments arranged by size: 3559 994 868 552 255 185 94 28 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5796 Fragments arranged by size: 5796 683 56 BtsI GCAGTG_nn' Cuts at: 4051 4845 4865 4051 Size: 794 20 5721 Fragments arranged by size: 5721 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6535 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6500 DraI TTT'AAA Cuts at: 2569 3839 2569 Size: 1270 5265 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5542 809 Size: 507 4226 1802 Fragments arranged by size: 4226 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2030 3381 3666 Size: 54 1425 50 275 18 200 1351 285 Cuts at: 3666 3982 4871 6500 8 Size: 316 889 1629 43 Fragments arranged by size: 1629 1425 1351 889 316 285 275 200 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2030 3982 1537 Size: 275 218 1952 4090 Fragments arranged by size: 4090 1952 275 218 EarI CTCTTCn'nnn_ Cuts at: 1353 4465 1353 Size: 3112 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5122 5496 5642 387 Size: 1064 138 3533 374 146 1280 Fragments arranged by size: 3533 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2520 2598 3457 4598 5195 5982 6250 Size: 393 888 78 859 1141 597 787 268 Cuts at: 6250 1239 Size: 1524 Fragments arranged by size: 1524 1141 888 859 787 597 393 268 78 EcoNI CCTnn'n_nnAGG Cuts at: 2210 2210 Size: 6535 EcoO109I rG'GnC_Cy Cuts at: 2055 2070 2071 2186 3825 2055 Size: 15 1 115 1639 4765 Fragments arranged by size: 4765 1639 115 15 1 EcoRI G'AATT_C Cuts at: 3844 4222 3844 Size: 378 6157 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2950 2950 Size: 6535 FspI TGC'GCA Cuts at: 5041 5041 Size: 6535 HaeII r_GCGC'y Cuts at: 1678 2136 3579 5682 1678 Size: 458 1443 2103 2531 Fragments arranged by size: 2531 2103 1443 458 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2156 2188 2836 2868 5176 Size: 32 135 32 584 32 648 32 2308 Cuts at: 5176 5208 5250 5282 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2308 648 584 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2419 3879 4125 669 Size: 988 281 481 1460 246 3079 Fragments arranged by size: 3079 1460 988 481 281 246 HindIII A'AGCT_T Cuts at: 748 3120 748 Size: 2372 4163 HpaI GTT'AAC Cuts at: 4125 4125 Size: 6535 KpnI G_GTAC'C Cuts at: 3860 3860 Size: 6535 MfeI C'AATT_G Cuts at: 4134 4134 Size: 6535 MluI A'CGCG_T Cuts at: 2681 6411 2681 Size: 3730 2805 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2693 3515 5649 5833 1305 Size: 1388 822 2134 184 2007 Fragments arranged by size: 2134 2007 1388 822 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3383 10 Size: 54 1425 343 1551 3162 Fragments arranged by size: 3162 1551 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4493 4852 5011 518 Size: 648 3327 359 159 2042 Fragments arranged by size: 3327 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2061 2089 2116 2155 2183 3787 3951 4628 Size: 455 28 27 39 28 1604 164 677 Cuts at: 4628 5776 6021 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1604 1148 677 455 245 164 39 28 28 27 NaeI GCC'GGC Cuts at: 2228 2228 Size: 6535 NcoI C'CATG_G Cuts at: 513 1065 3049 3234 3489 513 Size: 552 1984 185 255 3559 Fragments arranged by size: 3559 1984 552 255 185 NdeI CA'TA_TG Cuts at: 387 387 Size: 6535 NgoMIV G'CCGG_C Cuts at: 2226 2226 Size: 6535 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6535 NotI GC'GGCC_GC Cuts at: 3982 3982 Size: 6535 NspI r_CATG'y Cuts at: 1132 1229 2348 2663 3893 5438 1132 Size: 97 1119 315 1230 1545 2229 Fragments arranged by size: 2229 1545 1230 1119 315 97 PasI CC'CwG_GG Cuts at: 1348 3820 1348 Size: 2472 4063 PciI A'CATG_T Cuts at: 5434 5434 Size: 6535 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6535 PmeI GTTT'AAAC Cuts at: 3839 3839 Size: 6535 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4604 4636 6117 6149 4604 Size: 32 1481 32 4990 Fragments arranged by size: 4990 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6535 PsiI TTA'TAA Cuts at: 4105 4105 Size: 6535 PspOMI G'GGCC_C Cuts at: 2055 2070 2186 2055 Size: 15 116 6404 Fragments arranged by size: 6404 116 15 PstI C_TGCA'G Cuts at: 830 2172 3887 830 Size: 1342 1715 3478 Fragments arranged by size: 3478 1715 1342 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6535 PvuII CAG'CTG Cuts at: 3787 3787 Size: 6535 SacII CC_GC'GG Cuts at: 2062 2156 2184 2062 Size: 94 28 6413 Fragments arranged by size: 6413 94 28 SalI G'TCGA_C Cuts at: 1936 3877 1936 Size: 1941 4594 SbfI CC_TGCA'GG Cuts at: 3887 3887 Size: 6535 ScaI AGT'ACT Cuts at: 4783 6495 4783 Size: 1712 4823 SexAI A'CCwGG_T Cuts at: 3336 3336 Size: 6535 SfcI C'TryA_G Cuts at: 826 1045 2168 3883 5018 5699 5890 826 Size: 219 1123 1715 1135 681 191 1471 Fragments arranged by size: 1715 1471 1135 1123 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6535 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6535 SmaI CCC'GGG Cuts at: 1872 2052 3862 1872 Size: 180 1810 4545 Fragments arranged by size: 4545 1810 180 SmlI C'TyrA_G Cuts at: 820 1017 1943 2935 4639 5540 5802 820 Size: 197 926 992 1704 901 262 1553 Fragments arranged by size: 1704 1553 992 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4326 493 Size: 3833 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6535 SphI G_CATG'C Cuts at: 1132 2348 3893 1132 Size: 1216 1545 3774 Fragments arranged by size: 3774 1545 1216 SspI AAT'ATT Cuts at: 5 52 2724 2742 4459 5 Size: 47 2672 18 1717 2081 Fragments arranged by size: 2672 2081 1717 47 18 StuI AGG'CCT Cuts at: 1517 1517 Size: 6535 StyI C'CwwG_G Cuts at: 513 1065 2355 2619 2815 3049 3234 3489 3974 Size: 552 1290 264 196 234 185 255 485 Cuts at: 3974 513 Size: 3074 Fragments arranged by size: 3074 1290 552 485 264 255 234 196 185 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2225 2852 4562 4579 4732 4917 5362 Size: 1255 645 627 1710 17 153 185 445 Cuts at: 5362 325 Size: 1498 Fragments arranged by size: 1710 1498 1255 645 627 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2480 2656 3016 Size: 274 80 33 51 1443 503 176 360 Cuts at: 3016 4781 6493 96 Size: 1765 1712 138 Fragments arranged by size: 1765 1712 1443 503 360 274 176 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2367 2399 6495 6527 2367 Size: 32 4096 32 2375 Fragments arranged by size: 4096 2375 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6535 XbaI T'CTAG_A Cuts at: 3871 3871 Size: 6535 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6535 XmaI C'CCGG_G Cuts at: 1870 2050 3860 1870 Size: 180 1810 4545 Fragments arranged by size: 4545 1810 180 XmnI GAAnn'nnTTC Cuts at: 4664 4664 Size: 6535 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4343 276 Size: 53 83 186 936 2809 2468 Fragments arranged by size: 2809 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI XcmI