(Circular) (Six-base) MAPSORT of: pFN21AE0387.seq Check: 2159 from: 1 to: 6514 pFN21AE0387 6514 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3743 3743 Size: 6514 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4324 278 Size: 53 83 186 936 2788 2468 Fragments arranged by size: 2788 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2176 2553 3857 1937 Size: 239 377 1304 4594 Fragments arranged by size: 4594 1304 377 239 Acc65I G'GTAC_C Cuts at: 3835 3835 Size: 6514 AclI AA'CG_TT Cuts at: 1563 2189 3813 4641 5014 1563 Size: 626 1624 828 373 3063 Fragments arranged by size: 3063 1624 828 626 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2237 2545 2600 4577 5961 6229 1632 Size: 605 308 55 1977 1384 268 1917 Fragments arranged by size: 1977 1917 1384 605 308 268 55 AfeI AGC'GCT Cuts at: 2420 2420 Size: 6514 AflII C'TTAA_G Cuts at: 820 1017 3173 820 Size: 197 2156 4161 Fragments arranged by size: 4161 2156 197 AflIII A'CryG_T Cuts at: 1203 5413 6390 1203 Size: 4210 977 1327 Fragments arranged by size: 4210 1327 977 AgeI A'CCGG_T Cuts at: 5345 5345 Size: 6514 AhdI GACnn_n'nnGTC Cuts at: 5243 5243 Size: 6514 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6514 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6514 AlwNI CAG_nnn'CTG Cuts at: 2215 3766 4257 5829 2215 Size: 1551 491 1572 2900 Fragments arranged by size: 2900 1572 1551 491 ApaLI G'TGCA_C Cuts at: 4571 5727 4571 Size: 1156 5358 ApoI r'AATT_y Cuts at: 1493 3347 3800 3823 4049 4201 6310 1493 Size: 1854 453 23 226 152 2109 1697 Fragments arranged by size: 2109 1854 1697 453 226 152 23 AseI AT'TA_AT Cuts at: 160 1032 5068 160 Size: 872 4036 1606 Fragments arranged by size: 4036 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6514 AvaI C'yCGr_G Cuts at: 1870 1943 3839 1870 Size: 73 1896 4545 Fragments arranged by size: 4545 1896 73 BamHI G'GATC_C Cuts at: 2892 3844 4211 2892 Size: 952 367 5195 Fragments arranged by size: 5195 952 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2117 2146 2397 3835 5290 Size: 325 217 627 330 29 251 1438 1455 Cuts at: 5290 618 Size: 1842 Fragments arranged by size: 1842 1455 1438 627 330 325 251 217 29 BanII G_rGCy'C Cuts at: 1391 2168 3369 3446 3833 1391 Size: 777 1201 77 387 4072 Fragments arranged by size: 4072 1201 777 387 77 BbeI G_GCGC'C Cuts at: 2150 2150 Size: 6514 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2290 2601 928 Size: 971 391 311 4841 Fragments arranged by size: 4841 971 391 311 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4705 4739 1903 Size: 34 2768 34 3678 Fragments arranged by size: 3678 2768 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4406 5616 4406 Size: 1210 5304 BclI T'GATC_A Cuts at: 1548 1548 Size: 6514 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2684 5125 136 Size: 107 122 71 631 605 1012 2441 1525 Fragments arranged by size: 2441 1525 1012 631 605 122 107 71 BglII A'GATC_T Cuts at: 3024 6509 3024 Size: 3485 3029 BlpI GC'TnA_GC Cuts at: 2256 3931 2256 Size: 1675 4839 Bme1580I G_kGCm'C Cuts at: 1790 2795 3135 3407 4575 5731 1790 Size: 1005 340 272 1168 1156 2573 Fragments arranged by size: 2573 1168 1156 1005 340 272 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6514 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2610 2700 2881 Size: 416 53 83 399 24 1193 90 181 Cuts at: 2881 4245 5203 442 Size: 1364 958 1753 Fragments arranged by size: 1753 1364 1193 958 416 399 181 90 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6514 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2271 2271 Size: 6514 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2447 2508 2889 3085 3544 3564 5174 1239 Size: 1208 61 381 196 459 20 1610 2579 Fragments arranged by size: 2579 1610 1208 459 381 196 61 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4639 5504 5802 4639 Size: 865 298 5351 Fragments arranged by size: 5351 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3540 5177 882 Size: 617 2041 1637 2219 Fragments arranged by size: 2219 2041 1637 617 BsaAI yAC'GTr Cuts at: 493 1204 4305 493 Size: 711 3101 2702 Fragments arranged by size: 3101 2702 711 BsaBI GATnn'nnATC Cuts at: 2897 6508 2897 Size: 3611 2903 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2147 4321 4703 5338 Size: 53 83 186 936 614 2174 382 635 Cuts at: 5338 275 Size: 1451 Fragments arranged by size: 2174 1451 936 635 614 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2244 4947 5345 5619 5766 6177 2244 Size: 2703 398 274 147 411 2581 Fragments arranged by size: 2703 2581 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6289 Fragments arranged by size: 6289 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2477 1186 Size: 1291 5223 BseYI C'CCAG_C Cuts at: 2671 2927 3254 3552 3756 5717 6213 2671 Size: 256 327 298 204 1961 496 2972 Fragments arranged by size: 2972 1961 496 327 298 256 204 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3438 1163 Size: 573 1702 4239 Fragments arranged by size: 4239 1702 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3150 3964 4725 5753 1540 Size: 275 186 1149 814 761 1028 2301 Fragments arranged by size: 2301 1149 1028 814 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2511 2577 4575 4660 5731 2511 Size: 66 1998 85 1071 3294 Fragments arranged by size: 3294 1998 1071 85 66 BsmI GAATG_Cn' Cuts at: 2673 4025 4118 2673 Size: 1352 93 5069 Fragments arranged by size: 5069 1352 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2168 2511 2577 2795 3135 3369 3407 Size: 399 378 343 66 218 340 234 38 Cuts at: 3407 3446 3833 4575 4660 5731 1391 Size: 39 387 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 387 378 343 340 234 218 85 66 39 38 BspEI T'CCGG_A Cuts at: 2244 6177 2244 Size: 3933 2581 BspHI T'CATG_A Cuts at: 3726 4403 6133 3726 Size: 677 1730 4107 Fragments arranged by size: 4107 1730 677 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3348 3496 3743 3869 844 Size: 2504 148 247 126 3489 Fragments arranged by size: 3489 2504 247 148 126 BsrBI CCG'CTC Cuts at: 1394 1823 2104 4401 1394 Size: 429 281 2297 3507 Fragments arranged by size: 3507 2297 429 281 BsrDI GCAATG_nn' Cuts at: 65 1248 2763 5009 5183 65 Size: 1183 1515 2246 174 1396 Fragments arranged by size: 2246 1515 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2149 2957 3228 5158 5345 1700 Size: 110 339 808 271 1930 187 2869 Fragments arranged by size: 2869 1930 808 339 271 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6514 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6514 BssSI C'ACGA_G Cuts at: 4574 5586 4574 Size: 1012 5502 BstAPI GCAn_nnn'nTGC Cuts at: 794 2849 3699 794 Size: 2055 850 3609 Fragments arranged by size: 3609 2055 850 BstBI TT'CG_AA Cuts at: 2632 5318 2632 Size: 2686 3828 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6514 BstYI r'GATC_y Cuts at: 1972 2892 3024 3390 3720 3844 4211 4596 4613 Size: 920 132 366 330 124 367 385 17 Cuts at: 4613 6065 6151 6163 6509 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 920 385 367 366 346 330 132 124 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2973 3360 3478 513 Size: 552 1908 387 118 3549 Fragments arranged by size: 3549 1908 552 387 118 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5775 Fragments arranged by size: 5775 683 56 BtsI GCAGTG_nn' Cuts at: 3709 3767 4030 4824 4844 3709 Size: 58 263 794 20 5379 Fragments arranged by size: 5379 794 263 58 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6514 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6479 DraI TTT'AAA Cuts at: 3818 3818 Size: 6514 DraIII CAC_nnn'GTG Cuts at: 2506 2723 2506 Size: 217 6297 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5521 809 Size: 507 4205 1802 Fragments arranged by size: 4205 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2955 3147 3183 Size: 54 1425 50 275 18 1125 192 36 Cuts at: 3183 3961 4850 6479 8 Size: 778 889 1629 43 Fragments arranged by size: 1629 1425 1125 889 778 275 192 54 50 43 36 18 EagI C'GGCC_G Cuts at: 1537 1812 3147 3961 1537 Size: 275 1335 814 4090 Fragments arranged by size: 4090 1335 814 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4444 1353 Size: 3091 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2026 5101 5475 5621 387 Size: 1064 138 437 3075 374 146 1280 Fragments arranged by size: 3075 1280 1064 437 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2237 2447 2508 2545 2600 2889 3085 Size: 393 605 210 61 37 55 289 196 Cuts at: 3085 3544 3564 4577 5174 5961 6229 1239 Size: 459 20 1013 597 787 268 1524 Fragments arranged by size: 1524 1013 787 605 597 459 393 289 268 210 196 61 55 37 20 EcoNI CCTnn'n_nnAGG Cuts at: 3100 3100 Size: 6514 EcoO109I rG'GnC_Cy Cuts at: 2661 2676 3142 3337 3435 2661 Size: 15 466 195 98 5740 Fragments arranged by size: 5740 466 195 98 15 EcoRI G'AATT_C Cuts at: 3823 4201 3823 Size: 378 6136 EcoRV GAT'ATC Cuts at: 2392 2392 Size: 6514 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3589 3589 Size: 6514 FspI TGC'GCA Cuts at: 5020 5020 Size: 6514 HaeII r_GCGC'y Cuts at: 1678 2045 2093 2150 2422 3229 5661 1678 Size: 367 48 57 272 807 2432 2531 Fragments arranged by size: 2531 2432 807 367 272 57 48 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2302 2331 2334 2351 2363 Size: 32 135 32 730 29 3 17 12 Cuts at: 2363 2383 2462 2494 3519 3551 5155 5187 5229 Size: 20 79 32 1025 32 1604 32 42 Cuts at: 5229 5261 1373 Size: 32 2626 Fragments arranged by size: 2626 1604 1025 730 135 79 42 32 32 32 32 32 32 29 20 17 12 3 HincII GTy'rAC Cuts at: 669 1657 1938 2177 2554 2737 3858 4104 669 Size: 988 281 239 377 183 1121 246 3079 Fragments arranged by size: 3079 1121 988 377 281 246 239 183 HindIII A'AGCT_T Cuts at: 748 748 Size: 6514 HpaI GTT'AAC Cuts at: 4104 4104 Size: 6514 KasI G'GCGC_C Cuts at: 2146 2146 Size: 6514 KpnI G_GTAC'C Cuts at: 3839 3839 Size: 6514 MfeI C'AATT_G Cuts at: 4113 4113 Size: 6514 MluI A'CGCG_T Cuts at: 6390 6390 Size: 6514 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5628 5812 1305 Size: 4323 184 2007 Fragments arranged by size: 4323 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4692 Fragments arranged by size: 4692 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4472 4831 4990 518 Size: 648 3306 359 159 2042 Fragments arranged by size: 3306 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3254 3640 3690 3930 4607 5755 6000 1606 Size: 1648 386 50 240 677 1148 245 2120 Fragments arranged by size: 2120 1648 1148 677 386 245 240 50 NaeI GCC'GGC Cuts at: 2151 3230 2151 Size: 1079 5435 NarI GG'CG_CC Cuts at: 2147 2147 Size: 6514 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5962 NdeI CA'TA_TG Cuts at: 387 387 Size: 6514 NgoMIV G'CCGG_C Cuts at: 2149 3228 2149 Size: 1079 5435 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6514 NotI GC'GGCC_GC Cuts at: 3961 3961 Size: 6514 NspI r_CATG'y Cuts at: 1132 1229 3505 3872 5417 1132 Size: 97 2276 367 1545 2229 Fragments arranged by size: 2276 2229 1545 367 97 PasI CC'CwG_GG Cuts at: 1348 2123 1348 Size: 775 5739 PciI A'CATG_T Cuts at: 5413 5413 Size: 6514 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6514 PmeI GTTT'AAAC Cuts at: 3818 3818 Size: 6514 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4583 4615 6096 6128 4583 Size: 32 1481 32 4969 Fragments arranged by size: 4969 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2661 3142 3337 2661 Size: 481 195 5838 Fragments arranged by size: 5838 481 195 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6514 PsiI TTA'TAA Cuts at: 4084 4084 Size: 6514 PstI C_TGCA'G Cuts at: 830 2034 2916 3379 3704 3866 830 Size: 1204 882 463 325 162 3478 Fragments arranged by size: 3478 1204 882 463 325 162 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6514 PvuII CAG'CTG Cuts at: 3640 3690 3640 Size: 50 6464 SalI G'TCGA_C Cuts at: 1936 2175 2552 3856 1936 Size: 239 377 1304 4594 Fragments arranged by size: 4594 1304 377 239 SbfI CC_TGCA'GG Cuts at: 2034 3866 2034 Size: 1832 4682 ScaI AGT'ACT Cuts at: 4762 6474 4762 Size: 1712 4802 SfcI C'TryA_G Cuts at: 826 1045 2030 2912 3118 3375 3700 3862 4997 Size: 219 985 882 206 257 325 162 1135 Cuts at: 4997 5678 5869 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 985 882 681 325 257 219 206 191 162 SfoI GGC'GCC Cuts at: 2148 2148 Size: 6514 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6514 SgrAI Cr'CCGG_yG Cuts at: 1700 2149 1700 Size: 449 6065 SmaI CCC'GGG Cuts at: 1872 3841 1872 Size: 1969 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3173 4618 5519 5781 820 Size: 197 926 1230 1445 901 262 1553 Fragments arranged by size: 1553 1445 1230 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4305 493 Size: 3812 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6514 SphI G_CATG'C Cuts at: 1132 3872 1132 Size: 2740 3774 SspI AAT'ATT Cuts at: 5 52 4438 5 Size: 47 4386 2081 Fragments arranged by size: 4386 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6514 StyI C'CwwG_G Cuts at: 513 1065 3438 3953 513 Size: 552 2373 515 3074 Fragments arranged by size: 3074 2373 552 515 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2315 2415 3770 4541 4558 4711 4896 Size: 1255 735 100 1355 771 17 153 185 Cuts at: 4896 5341 325 Size: 445 1498 Fragments arranged by size: 1498 1355 1255 771 735 445 185 153 100 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2545 2880 3213 Size: 274 80 33 51 1443 568 335 333 Cuts at: 3213 4760 6472 96 Size: 1547 1712 138 Fragments arranged by size: 1712 1547 1443 568 335 333 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2969 3001 6474 6506 2969 Size: 32 3473 32 2977 Fragments arranged by size: 3473 2977 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6514 XbaI T'CTAG_A Cuts at: 3850 3850 Size: 6514 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3251 3680 3251 Size: 429 6085 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6514 XmaI C'CCGG_G Cuts at: 1870 3839 1870 Size: 1969 4545 XmnI GAAnn'nnTTC Cuts at: 2987 4643 2987 Size: 1656 4858 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4322 276 Size: 53 83 186 936 2788 2468 Fragments arranged by size: 2788 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI