(Circular) (Six-base) MAPSORT of: pFN21AE0385.seq Check: 6699 from: 1 to: 7000 pFN21AE0385 7000 bp With 182 enzymes: SgfI * February 7, 2012 16:05 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4810 278 Size: 53 83 186 936 3274 2468 Fragments arranged by size: 3274 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2691 4343 1937 Size: 754 1652 4594 Fragments arranged by size: 4594 1652 754 Acc65I G'GTAC_C Cuts at: 3119 4321 3119 Size: 1202 5798 AclI AA'CG_TT Cuts at: 1563 5127 5500 1563 Size: 3564 373 3063 Fragments arranged by size: 3564 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2562 3162 5063 6447 6715 1632 Size: 930 600 1901 1384 268 1917 Fragments arranged by size: 1917 1901 1384 930 600 268 AfeI AGC'GCT Cuts at: 2083 2083 Size: 7000 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6803 AflIII A'CryG_T Cuts at: 1203 3028 3564 3787 5899 6876 1203 Size: 1825 536 223 2112 977 1327 Fragments arranged by size: 2112 1825 1327 977 536 223 AgeI A'CCGG_T Cuts at: 5831 5831 Size: 7000 AhdI GACnn_n'nnGTC Cuts at: 3327 5729 3327 Size: 2402 4598 AleI CACnn'nnGTG Cuts at: 1166 2326 2616 1166 Size: 1160 290 5550 Fragments arranged by size: 5550 1160 290 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7000 AlwNI CAG_nnn'CTG Cuts at: 2306 4743 6315 2306 Size: 2437 1572 2991 Fragments arranged by size: 2991 2437 1572 ApaLI G'TGCA_C Cuts at: 5057 6213 5057 Size: 1156 5844 ApoI r'AATT_y Cuts at: 1493 2123 2956 3231 3459 4309 4535 4687 6796 Size: 630 833 275 228 850 226 152 2109 Cuts at: 6796 1493 Size: 1697 Fragments arranged by size: 2109 1697 850 833 630 275 228 226 152 AseI AT'TA_AT Cuts at: 160 1032 3477 3899 4022 5554 160 Size: 872 2445 422 123 1532 1606 Fragments arranged by size: 2445 1606 1532 872 422 123 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7000 AvaI C'yCGr_G Cuts at: 1870 1943 4325 1870 Size: 73 2382 4545 Fragments arranged by size: 4545 2382 73 AvrII C'CTAG_G Cuts at: 3203 3203 Size: 7000 BamHI G'GATC_C Cuts at: 2918 4330 4697 2918 Size: 1412 367 5221 Fragments arranged by size: 5221 1412 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3119 4321 5776 618 Size: 325 217 627 1332 1202 1455 1842 Fragments arranged by size: 1842 1455 1332 1202 627 325 217 BanII G_rGCy'C Cuts at: 1391 4319 1391 Size: 2928 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2705 928 Size: 971 806 5223 Fragments arranged by size: 5223 971 806 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5191 5225 1903 Size: 34 3254 34 3678 Fragments arranged by size: 3678 3254 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3838 4892 6102 3838 Size: 1054 1210 4736 Fragments arranged by size: 4736 1210 1054 BclI T'GATC_A Cuts at: 1548 3679 1548 Size: 2131 4869 BfrBI ATG'CAT Cuts at: 3703 3703 Size: 7000 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2046 5611 136 Size: 107 122 71 631 605 374 3565 1525 Fragments arranged by size: 3565 1525 631 605 374 122 107 71 BglII A'GATC_T Cuts at: 3258 6995 3258 Size: 3737 3263 BlpI GC'TnA_GC Cuts at: 4417 4417 Size: 7000 Bme1580I G_kGCm'C Cuts at: 1790 2756 5061 6217 1790 Size: 966 2305 1156 2573 Fragments arranged by size: 2573 2305 1156 966 BmgBI CAC'GTC Cuts at: 1318 2029 3031 1318 Size: 711 1002 5287 Fragments arranged by size: 5287 1002 711 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4731 5689 442 Size: 416 53 83 399 24 3314 958 1753 Fragments arranged by size: 3314 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7000 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3265 3265 Size: 7000 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2454 3060 3531 4176 5660 1239 Size: 1215 606 471 645 1484 2579 Fragments arranged by size: 2579 1484 1215 645 606 471 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3257 3642 5125 5990 6288 3257 Size: 385 1483 865 298 3969 Fragments arranged by size: 3969 1483 865 385 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4029 5663 882 Size: 617 2530 1634 2219 Fragments arranged by size: 2530 2219 1634 617 BsaAI yAC'GTr Cuts at: 493 1204 2866 4791 493 Size: 711 1662 1925 2702 Fragments arranged by size: 2702 1925 1662 711 BsaBI GATnn'nnATC Cuts at: 4024 6994 4024 Size: 2970 4030 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4051 4807 5189 5824 Size: 53 83 186 936 2518 756 382 635 Cuts at: 5824 275 Size: 1451 Fragments arranged by size: 2518 1451 936 756 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5433 5831 6105 6252 6663 5433 Size: 398 274 147 411 5770 Fragments arranged by size: 5770 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2789 2819 3033 3036 3063 Size: 30 165 30 1386 30 214 3 27 Cuts at: 3063 3066 1178 Size: 3 5112 Fragments arranged by size: 5112 1386 214 165 30 30 30 27 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2451 3057 3254 1186 Size: 838 427 606 197 4932 Fragments arranged by size: 4932 838 606 427 197 BseYI C'CCAG_C Cuts at: 2078 2306 6203 6699 2078 Size: 228 3897 496 2379 Fragments arranged by size: 3897 2379 496 228 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6427 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3451 4450 5211 6239 1540 Size: 275 186 1450 999 761 1028 2301 Fragments arranged by size: 2301 1450 1028 999 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2385 2563 2911 2944 3023 5061 5146 6217 2385 Size: 178 348 33 79 2038 85 1071 3168 Fragments arranged by size: 3168 2038 1071 348 178 85 79 33 BsmI GAATG_Cn' Cuts at: 3766 4511 4604 3766 Size: 745 93 6162 Fragments arranged by size: 6162 745 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2385 2563 2756 2911 2944 3023 4319 Size: 399 595 178 193 155 33 79 1296 Cuts at: 4319 5061 5146 6217 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1296 1071 742 595 399 193 178 155 85 79 33 BspEI T'CCGG_A Cuts at: 6663 6663 Size: 7000 BspHI T'CATG_A Cuts at: 2551 2847 4231 4889 6619 2551 Size: 296 1384 658 1730 2932 Fragments arranged by size: 2932 1730 1384 658 296 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4120 4355 844 Size: 3276 235 3489 Fragments arranged by size: 3489 3276 235 BsrBI CCG'CTC Cuts at: 1394 1823 4887 1394 Size: 429 3064 3507 Fragments arranged by size: 3507 3064 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2586 5495 5669 65 Size: 1183 1338 2909 174 1396 Fragments arranged by size: 2909 1396 1338 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2036 2065 5644 5831 1700 Size: 110 226 29 3579 187 2869 Fragments arranged by size: 3579 2869 226 187 110 29 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7000 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7000 BssSI C'ACGA_G Cuts at: 5060 6072 5060 Size: 1012 5988 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7000 BstBI TT'CG_AA Cuts at: 2148 5804 2148 Size: 3656 3344 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7000 BstXI CCAn_nnnn'nTGG Cuts at: 3331 3647 3725 3331 Size: 316 78 6606 Fragments arranged by size: 6606 316 78 BstYI r'GATC_y Cuts at: 1972 2918 3258 3740 4330 4697 5082 5099 6551 Size: 946 340 482 590 367 385 17 1452 Cuts at: 6551 6637 6649 6995 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 946 590 482 385 367 346 340 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2756 3646 3718 3736 513 Size: 552 939 752 890 72 18 3777 Fragments arranged by size: 3777 939 890 752 552 72 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3847 487 Size: 683 56 2621 3640 Fragments arranged by size: 3640 2621 683 56 BtsI GCAGTG_nn' Cuts at: 4516 5310 5330 4516 Size: 794 20 6186 Fragments arranged by size: 6186 794 20 ClaI AT'CG_AT Cuts at: 1554 3197 1554 Size: 1643 5357 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4161 4196 529 Size: 35 3597 35 3333 Fragments arranged by size: 3597 3333 35 35 DraI TTT'AAA Cuts at: 2293 4304 2293 Size: 2011 4989 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6007 809 Size: 507 4691 1802 Fragments arranged by size: 4691 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 3448 4447 Size: 54 1425 50 275 18 204 1414 999 Cuts at: 4447 5336 6965 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1414 999 889 275 204 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3448 4447 1537 Size: 275 1636 999 4090 Fragments arranged by size: 4090 1636 999 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4930 1353 Size: 3577 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5587 5961 6107 387 Size: 1064 138 3998 374 146 1280 Fragments arranged by size: 3998 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2454 2562 3060 3162 3531 4176 5063 Size: 393 822 108 498 102 369 645 887 Cuts at: 5063 5660 6447 6715 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 887 822 787 645 597 498 393 369 268 108 102 EcoRI G'AATT_C Cuts at: 4309 4687 4309 Size: 378 6622 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3140 3140 Size: 7000 FseI GG_CCGG'CC Cuts at: 2040 2040 Size: 7000 FspI TGC'GCA Cuts at: 5506 5506 Size: 7000 HaeII r_GCGC'y Cuts at: 1678 2085 6147 1678 Size: 407 4062 2531 Fragments arranged by size: 4062 2531 407 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3313 3345 3529 3561 3846 Size: 32 135 32 1741 32 184 32 285 Cuts at: 3846 3878 5641 5673 5715 5747 1373 Size: 32 1763 32 42 32 2626 Fragments arranged by size: 2626 1763 1741 285 184 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2692 3570 4344 4590 669 Size: 988 281 754 878 774 246 3079 Fragments arranged by size: 3079 988 878 774 754 281 246 HindIII A'AGCT_T Cuts at: 748 2718 748 Size: 1970 5030 HpaI GTT'AAC Cuts at: 3570 4590 3570 Size: 1020 5980 KpnI G_GTAC'C Cuts at: 3123 4325 3123 Size: 1202 5798 MfeI C'AATT_G Cuts at: 4599 4599 Size: 7000 MluI A'CGCG_T Cuts at: 6876 6876 Size: 7000 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3565 6114 6298 1305 Size: 2260 2549 184 2007 Fragments arranged by size: 2549 2260 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5178 Fragments arranged by size: 5178 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2326 2556 2616 3563 3639 3645 3702 Size: 648 1160 230 60 947 76 6 57 Cuts at: 3702 3723 3818 4958 5317 5476 518 Size: 21 95 1140 359 159 2042 Fragments arranged by size: 2042 1160 1140 947 648 359 230 159 95 76 60 57 21 6 MspA1I CmG'CkG Cuts at: 1606 4416 5093 6241 6486 1606 Size: 2810 677 1148 245 2120 Fragments arranged by size: 2810 2120 1148 677 245 NaeI GCC'GGC Cuts at: 2038 2038 Size: 7000 NcoI C'CATG_G Cuts at: 513 1065 2004 2756 3646 3718 513 Size: 552 939 752 890 72 3795 Fragments arranged by size: 3795 939 890 752 552 72 NdeI CA'TA_TG Cuts at: 387 2155 2571 3699 4206 387 Size: 1768 416 1128 507 3181 Fragments arranged by size: 3181 1768 1128 507 416 NgoMIV G'CCGG_C Cuts at: 2036 2036 Size: 7000 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7000 NotI GC'GGCC_GC Cuts at: 4447 4447 Size: 7000 NsiI A_TGCA'T Cuts at: 3705 3705 Size: 7000 NspI r_CATG'y Cuts at: 1132 1229 2300 3361 3568 3791 4358 5903 1132 Size: 97 1071 1061 207 223 567 1545 2229 Fragments arranged by size: 2229 1545 1071 1061 567 223 207 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7000 PciI A'CATG_T Cuts at: 3564 3787 5899 3564 Size: 223 2112 4665 Fragments arranged by size: 4665 2112 223 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7000 PfoI T'CCnGG_A Cuts at: 2853 2853 Size: 7000 PmeI GTTT'AAAC Cuts at: 4304 4304 Size: 7000 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5069 5101 6582 6614 5069 Size: 32 1481 32 5455 Fragments arranged by size: 5455 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7000 PsiI TTA'TAA Cuts at: 3159 4570 3159 Size: 1411 5589 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3819 3851 3819 Size: 32 6968 PstI C_TGCA'G Cuts at: 830 4352 830 Size: 3522 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7000 SalI G'TCGA_C Cuts at: 1936 2690 4342 1936 Size: 754 1652 4594 Fragments arranged by size: 4594 1652 754 SbfI CC_TGCA'GG Cuts at: 4352 4352 Size: 7000 ScaI AGT'ACT Cuts at: 2637 4222 5248 6960 2637 Size: 1585 1026 1712 2677 Fragments arranged by size: 2677 1712 1585 1026 SfcI C'TryA_G Cuts at: 826 1045 4348 5483 6164 6355 826 Size: 219 3303 1135 681 191 1471 Fragments arranged by size: 3303 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7000 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7000 SmaI CCC'GGG Cuts at: 1872 4327 1872 Size: 2455 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3236 3657 5104 6005 6267 820 Size: 197 926 1293 421 1447 901 262 1553 Fragments arranged by size: 1553 1447 1293 926 901 421 262 197 SnaBI TAC'GTA Cuts at: 493 4791 493 Size: 4298 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7000 SphI G_CATG'C Cuts at: 1132 4358 1132 Size: 3226 3774 SspI AAT'ATT Cuts at: 5 52 2289 3903 4924 5 Size: 47 2237 1614 1021 2081 Fragments arranged by size: 2237 2081 1614 1021 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7000 StyI C'CwwG_G Cuts at: 513 1065 2004 2059 2756 3203 3646 3718 4439 Size: 552 939 55 697 447 443 72 721 Cuts at: 4439 513 Size: 3074 Fragments arranged by size: 3074 939 721 697 552 447 443 72 55 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2298 3158 5027 5044 5197 5382 5827 Size: 1255 718 860 1869 17 153 185 445 Cuts at: 5827 325 Size: 1498 Fragments arranged by size: 1869 1498 1255 860 718 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2635 4123 4220 Size: 274 80 33 51 1443 658 1488 97 Cuts at: 4220 5246 6958 96 Size: 1026 1712 138 Fragments arranged by size: 1712 1488 1443 1026 658 274 138 97 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3036 3068 6960 6992 3036 Size: 32 3892 32 3044 Fragments arranged by size: 3892 3044 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7000 XbaI T'CTAG_A Cuts at: 4336 4336 Size: 7000 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7000 XmaI C'CCGG_G Cuts at: 1870 4325 1870 Size: 2455 4545 XmnI GAAnn'nnTTC Cuts at: 5129 5129 Size: 7000 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4808 276 Size: 53 83 186 936 3274 2468 Fragments arranged by size: 3274 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FspAI KasI NarI NruI PacI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI