(Circular) (Six-base) MAPSORT of: pFN21AE0384.seq Check: 9855 from: 1 to: 6172 pFN21AE0384 6172 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2790 3982 278 Size: 53 83 186 936 1254 1192 2468 Fragments arranged by size: 2468 1254 1192 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3515 1937 Size: 1578 4594 Acc65I G'GTAC_C Cuts at: 3493 3493 Size: 6172 AclI AA'CG_TT Cuts at: 1563 4299 4672 1563 Size: 2736 373 3063 Fragments arranged by size: 3063 2736 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3113 4235 5619 5887 1632 Size: 1481 1122 1384 268 1917 Fragments arranged by size: 1917 1481 1384 1122 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5975 AflIII A'CryG_T Cuts at: 1203 2883 5071 6048 1203 Size: 1680 2188 977 1327 Fragments arranged by size: 2188 1680 1327 977 AgeI A'CCGG_T Cuts at: 5003 5003 Size: 6172 AhdI GACnn_n'nnGTC Cuts at: 4901 4901 Size: 6172 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6172 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2960 1184 Size: 1776 4396 AlwNI CAG_nnn'CTG Cuts at: 2645 2698 2710 3346 3915 5487 2645 Size: 53 12 636 569 1572 3330 Fragments arranged by size: 3330 1572 636 569 53 12 ApaI G_GGCC'C Cuts at: 2135 2184 2238 2293 2493 2520 2135 Size: 49 54 55 200 27 5787 Fragments arranged by size: 5787 200 55 54 49 27 ApaLI G'TGCA_C Cuts at: 2257 4229 5385 2257 Size: 1972 1156 3044 Fragments arranged by size: 3044 1972 1156 ApoI r'AATT_y Cuts at: 1493 3481 3707 3859 5968 1493 Size: 1988 226 152 2109 1697 Fragments arranged by size: 2109 1988 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4726 160 Size: 872 3694 1606 Fragments arranged by size: 3694 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6172 AvaI C'yCGr_G Cuts at: 1870 1943 2110 2128 2195 2231 2912 2999 3497 Size: 73 167 18 67 36 681 87 498 Cuts at: 3497 1870 Size: 4545 Fragments arranged by size: 4545 681 498 167 87 73 67 36 18 BamHI G'GATC_C Cuts at: 3225 3502 3869 3225 Size: 277 367 5528 Fragments arranged by size: 5528 367 277 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2021 2087 2114 2200 2483 Size: 325 217 627 234 66 27 86 283 Cuts at: 2483 2963 2975 3108 3204 3493 4948 618 Size: 480 12 133 96 289 1455 1842 Fragments arranged by size: 1842 1455 627 480 325 289 283 234 217 133 96 86 66 27 12 BanII G_rGCy'C Cuts at: 1391 2135 2184 2238 2293 2433 2493 2520 2919 Size: 744 49 54 55 140 60 27 399 Cuts at: 2919 3045 3182 3491 1391 Size: 126 137 309 4072 Fragments arranged by size: 4072 744 399 309 140 137 126 60 55 54 49 27 BbeI G_GCGC'C Cuts at: 2091 2118 2487 2091 Size: 27 369 5776 Fragments arranged by size: 5776 369 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3405 928 Size: 971 1506 3695 Fragments arranged by size: 3695 1506 971 BbvCI CC'TCA_GC Cuts at: 2738 2827 2738 Size: 89 6083 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4363 4397 1903 Size: 34 50 34 2342 34 3678 Fragments arranged by size: 3678 2342 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3244 4064 5274 3244 Size: 820 1210 4142 Fragments arranged by size: 4142 1210 820 BclI T'GATC_A Cuts at: 1548 3057 1548 Size: 1509 4663 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2066 2233 Size: 107 122 71 631 605 337 57 167 Cuts at: 2233 2481 3281 4783 136 Size: 248 800 1502 1525 Fragments arranged by size: 1525 1502 800 631 605 337 248 167 122 107 71 57 BglII A'GATC_T Cuts at: 6167 6167 Size: 6172 BlpI GC'TnA_GC Cuts at: 2808 3589 2808 Size: 781 5391 Bme1580I G_kGCm'C Cuts at: 1790 2052 2135 2184 2203 2238 2261 2293 2493 Size: 262 83 49 19 35 23 32 200 Cuts at: 2493 2520 2655 2978 3000 4233 5389 1790 Size: 27 135 323 22 1233 1156 2573 Fragments arranged by size: 2573 1233 1156 323 262 200 135 83 49 35 32 27 23 22 19 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6172 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2633 3298 3903 Size: 416 53 83 399 24 1216 665 605 Cuts at: 3903 4861 442 Size: 958 1753 Fragments arranged by size: 1753 1216 958 665 605 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6172 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2462 2564 2570 2612 2705 2738 2826 2834 Size: 1223 102 6 42 93 33 88 8 Cuts at: 2834 4832 1239 Size: 1998 2579 Fragments arranged by size: 2579 1998 1223 102 93 88 42 33 8 6 Bpu10I CC'TnA_GC Cuts at: 2147 2738 2827 3086 2147 Size: 591 89 259 5233 Fragments arranged by size: 5233 591 259 89 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4297 5162 5460 4297 Size: 865 298 5009 Fragments arranged by size: 5009 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4835 882 Size: 617 3336 2219 Fragments arranged by size: 3336 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3963 493 Size: 711 2759 2702 Fragments arranged by size: 2759 2702 711 BsaBI GATnn'nnATC Cuts at: 2861 3230 6166 2861 Size: 369 2936 2867 Fragments arranged by size: 2936 2867 369 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2088 2115 2484 2556 Size: 53 83 186 936 555 27 369 72 Cuts at: 2556 2787 3979 4361 4996 275 Size: 231 1192 382 635 1451 Fragments arranged by size: 1451 1192 936 635 555 382 369 231 186 83 72 53 27 BsaWI w'CCGG_w Cuts at: 3182 4605 5003 5277 5424 5835 3182 Size: 1423 398 274 147 411 3519 Fragments arranged by size: 3519 1423 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2807 2837 1178 Size: 30 165 30 1404 30 4513 Fragments arranged by size: 4513 1404 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6172 BseYI C'CCAG_C Cuts at: 2292 2443 2632 3075 3376 5375 5871 2292 Size: 151 189 443 301 1999 496 2593 Fragments arranged by size: 2593 1999 496 443 301 189 151 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2141 3346 1163 Size: 573 405 1205 3989 Fragments arranged by size: 3989 1205 573 405 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2106 2361 2587 3622 4383 5411 Size: 275 186 105 255 226 1035 761 1028 Cuts at: 5411 1540 Size: 2301 Fragments arranged by size: 2301 1035 1028 761 275 255 226 186 105 BsiHKAI G_wGCw'C Cuts at: 2261 2357 2433 2820 2919 4233 4318 5389 2261 Size: 96 76 387 99 1314 85 1071 3044 Fragments arranged by size: 3044 1314 1071 387 99 96 85 76 BsmI GAATG_Cn' Cuts at: 2924 3683 3776 2924 Size: 759 93 5320 Fragments arranged by size: 5320 759 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2135 2184 2203 2238 2261 2293 Size: 399 262 83 49 19 35 23 32 Cuts at: 2293 2357 2433 2493 2520 2655 2820 2919 2978 Size: 64 76 60 27 135 165 99 59 Cuts at: 2978 3000 3045 3182 3491 4233 4318 5389 1391 Size: 22 45 137 309 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 309 262 165 137 135 99 85 83 76 64 60 59 49 45 35 32 27 23 22 19 BspEI T'CCGG_A Cuts at: 5835 5835 Size: 6172 BspHI T'CATG_A Cuts at: 4061 5791 4061 Size: 1730 4442 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3527 844 Size: 2683 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2460 4059 1394 Size: 429 637 1599 3507 Fragments arranged by size: 3507 1599 637 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3297 3351 4667 4841 65 Size: 1183 2049 54 1316 174 1396 Fragments arranged by size: 2049 1396 1316 1183 174 54 BsrFI r'CCGG_y Cuts at: 1700 1810 2435 2657 2754 2966 3264 4816 5003 Size: 110 625 222 97 212 298 1552 187 Cuts at: 5003 1700 Size: 2869 Fragments arranged by size: 2869 1552 625 298 222 212 187 110 97 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6172 BssHII G'CGCG_C Cuts at: 1925 2151 2214 2396 2563 1925 Size: 226 63 182 167 5534 Fragments arranged by size: 5534 226 182 167 63 BssSI C'ACGA_G Cuts at: 4232 5244 4232 Size: 1012 5160 BstAPI GCAn_nnn'nTGC Cuts at: 794 2224 794 Size: 1430 4742 BstBI TT'CG_AA Cuts at: 4976 4976 Size: 6172 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6172 BstYI r'GATC_y Cuts at: 1972 3225 3502 3869 4254 4271 5723 5809 5821 Size: 1253 277 367 385 17 1452 86 12 Cuts at: 5821 6167 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1253 385 367 346 277 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2205 2505 3045 513 Size: 552 1140 300 540 3640 Fragments arranged by size: 3640 1140 552 540 300 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2023 3106 487 Size: 683 56 797 1083 3553 Fragments arranged by size: 3553 1083 797 683 56 BtsI GCAGTG_nn' Cuts at: 3363 3371 3688 4482 4502 3363 Size: 8 317 794 20 5033 Fragments arranged by size: 5033 794 317 20 8 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6172 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6137 DraI TTT'AAA Cuts at: 3476 3476 Size: 6172 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5179 809 Size: 507 3863 1802 Fragments arranged by size: 3863 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 2208 2358 Size: 54 1425 50 275 18 228 150 150 Cuts at: 2358 2508 2968 3262 3619 4508 6137 8 Size: 150 460 294 357 889 1629 43 Fragments arranged by size: 1629 1425 889 460 357 294 275 228 150 150 150 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2358 3619 1537 Size: 275 546 1261 4090 Fragments arranged by size: 4090 1261 546 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2328 4102 1353 Size: 975 1774 3423 Fragments arranged by size: 3423 1774 975 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4759 5133 5279 387 Size: 1064 138 3170 374 146 1280 Fragments arranged by size: 3170 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2431 2917 2431 Size: 486 5686 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2462 2564 2570 2612 2705 2738 2826 Size: 393 830 102 6 42 93 33 88 Cuts at: 2826 2834 3113 4235 4832 5619 5887 1239 Size: 8 279 1122 597 787 268 1524 Fragments arranged by size: 1524 1122 830 787 597 393 279 268 102 93 88 42 33 8 6 EcoNI CCTnn'n_nnAGG Cuts at: 2594 2594 Size: 6172 EcoO109I rG'GnC_Cy Cuts at: 2181 2235 2289 2490 2517 2823 3343 2181 Size: 54 54 201 27 306 520 5010 Fragments arranged by size: 5010 520 306 201 54 54 27 EcoRI G'AATT_C Cuts at: 3481 3859 3481 Size: 378 5794 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3457 3457 Size: 6172 FspI TGC'GCA Cuts at: 4678 4678 Size: 6172 HaeII r_GCGC'y Cuts at: 1678 2078 2091 2118 2316 2384 2487 5319 1678 Size: 400 13 27 198 68 103 2832 2531 Fragments arranged by size: 2832 2531 400 198 103 68 27 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2807 2839 4813 4845 4887 Size: 32 135 32 1235 32 1974 32 42 Cuts at: 4887 4919 1373 Size: 32 2626 Fragments arranged by size: 2626 1974 1235 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3516 3762 669 Size: 988 281 1578 246 3079 Fragments arranged by size: 3079 1578 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6172 HpaI GTT'AAC Cuts at: 3762 3762 Size: 6172 KasI G'GCGC_C Cuts at: 2087 2114 2483 2087 Size: 27 369 5776 Fragments arranged by size: 5776 369 27 KpnI G_GTAC'C Cuts at: 3497 3497 Size: 6172 MfeI C'AATT_G Cuts at: 3771 3771 Size: 6172 MluI A'CGCG_T Cuts at: 6048 6048 Size: 6172 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2763 5286 5470 1305 Size: 1458 2523 184 2007 Fragments arranged by size: 2523 2007 1458 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4350 Fragments arranged by size: 4350 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4130 4489 4648 518 Size: 648 2964 359 159 2042 Fragments arranged by size: 2964 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2140 2207 2224 2266 2368 2451 2627 2683 Size: 534 67 17 42 102 83 176 56 Cuts at: 2683 2695 2961 3079 3588 4265 5413 5658 1606 Size: 12 266 118 509 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 534 509 266 245 176 118 102 83 67 56 42 17 12 NaeI GCC'GGC Cuts at: 2756 2756 Size: 6172 NarI GG'CG_CC Cuts at: 2088 2115 2484 2088 Size: 27 369 5776 Fragments arranged by size: 5776 369 27 NcoI C'CATG_G Cuts at: 513 1065 2505 3045 513 Size: 552 1440 540 3640 Fragments arranged by size: 3640 1440 552 540 NdeI CA'TA_TG Cuts at: 387 387 Size: 6172 NgoMIV G'CCGG_C Cuts at: 2754 2754 Size: 6172 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6172 NotI GC'GGCC_GC Cuts at: 3619 3619 Size: 6172 NspI r_CATG'y Cuts at: 1132 1229 2887 2941 3530 5075 1132 Size: 97 1658 54 589 1545 2229 Fragments arranged by size: 2229 1658 1545 589 97 54 PasI CC'CwG_GG Cuts at: 1348 2520 3256 3393 1348 Size: 1172 736 137 4127 Fragments arranged by size: 4127 1172 736 137 PciI A'CATG_T Cuts at: 2883 5071 2883 Size: 2188 3984 PflMI CCAn_nnn'nTGG Cuts at: 1108 2710 3346 1108 Size: 1602 636 3934 Fragments arranged by size: 3934 1602 636 PmeI GTTT'AAAC Cuts at: 3476 3476 Size: 6172 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4241 4273 5754 5786 4241 Size: 32 1481 32 4627 Fragments arranged by size: 4627 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2823 3343 2823 Size: 520 5652 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6172 PsiI TTA'TAA Cuts at: 3742 3742 Size: 6172 PspOMI G'GGCC_C Cuts at: 2131 2180 2234 2289 2489 2516 2131 Size: 49 54 55 200 27 5787 Fragments arranged by size: 5787 200 55 54 49 27 PstI C_TGCA'G Cuts at: 830 2352 2388 2796 3177 3524 830 Size: 1522 36 408 381 347 3478 Fragments arranged by size: 3478 1522 408 381 347 36 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6172 PvuII CAG'CTG Cuts at: 2368 2627 2683 2695 3079 2368 Size: 259 56 12 384 5461 Fragments arranged by size: 5461 384 259 56 12 SacI G_AGCT'C Cuts at: 2433 2919 2433 Size: 486 5686 SacII CC_GC'GG Cuts at: 2208 2208 Size: 6172 SalI G'TCGA_C Cuts at: 1936 3514 1936 Size: 1578 4594 SapI GCTCTTCn'nnn_ Cuts at: 2328 2328 Size: 6172 SbfI CC_TGCA'GG Cuts at: 2796 3524 2796 Size: 728 5444 ScaI AGT'ACT Cuts at: 4420 6132 4420 Size: 1712 4460 SfcI C'TryA_G Cuts at: 826 1045 2348 2384 2792 2833 3173 3520 4655 Size: 219 1303 36 408 41 340 347 1135 Cuts at: 4655 5336 5527 826 Size: 681 191 1471 Fragments arranged by size: 1471 1303 1135 681 408 347 340 219 191 41 36 SfoI GGC'GCC Cuts at: 2089 2116 2485 2089 Size: 27 369 5776 Fragments arranged by size: 5776 369 27 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6172 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6172 SmaI CCC'GGG Cuts at: 1872 2130 2197 2233 3001 3499 1872 Size: 258 67 36 768 498 4545 Fragments arranged by size: 4545 768 498 258 67 36 SmlI C'TyrA_G Cuts at: 820 1017 1943 4276 5177 5439 820 Size: 197 926 2333 901 262 1553 Fragments arranged by size: 2333 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3963 493 Size: 3470 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6172 SphI G_CATG'C Cuts at: 1132 3530 1132 Size: 2398 3774 SrfI GCCC'GGGC Cuts at: 2233 3001 2233 Size: 768 5404 SspI AAT'ATT Cuts at: 5 52 4096 5 Size: 47 4044 2081 Fragments arranged by size: 4044 2081 47 StuI AGG'CCT Cuts at: 1517 3284 3416 1517 Size: 1767 132 4273 Fragments arranged by size: 4273 1767 132 StyI C'CwwG_G Cuts at: 513 1065 2505 3045 3465 3611 513 Size: 552 1440 540 420 146 3074 Fragments arranged by size: 3074 1440 552 540 420 146 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2092 3248 3293 4199 4216 4369 4554 Size: 1255 512 1156 45 906 17 153 185 Cuts at: 4554 4999 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1156 906 512 445 185 153 45 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3019 4418 6130 Size: 274 80 33 51 1443 1042 1399 1712 Cuts at: 6130 96 Size: 138 Fragments arranged by size: 1712 1443 1399 1042 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6132 6164 6132 Size: 32 6140 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6172 XbaI T'CTAG_A Cuts at: 3508 3508 Size: 6172 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2050 2050 Size: 6172 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6172 XmaI C'CCGG_G Cuts at: 1870 2128 2195 2231 2999 3497 1870 Size: 258 67 36 768 498 4545 Fragments arranged by size: 4545 768 498 258 67 36 XmnI GAAnn'nnTTC Cuts at: 4301 4301 Size: 6172 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2788 3980 276 Size: 53 83 186 936 1254 1192 2468 Fragments arranged by size: 2468 1254 1192 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SanDI SexAI SfiI SwaI