(Circular) (Six-base) MAPSORT of: pFN21AE0383.seq Check: 4984 from: 1 to: 7849 pFN21AE0383 7849 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3485 3485 Size: 7849 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5659 278 Size: 53 83 186 936 4123 2468 Fragments arranged by size: 4123 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2605 5192 1937 Size: 668 2587 4594 Fragments arranged by size: 4594 2587 668 Acc65I G'GTAC_C Cuts at: 5170 5170 Size: 7849 AclI AA'CG_TT Cuts at: 1563 5976 6349 1563 Size: 4413 373 3063 Fragments arranged by size: 4413 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3971 4065 4832 4868 5912 7296 7564 1632 Size: 2339 94 767 36 1044 1384 268 1917 Fragments arranged by size: 2339 1917 1384 1044 767 268 94 36 AfeI AGC'GCT Cuts at: 2714 2714 Size: 7849 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7652 AflIII A'CryG_T Cuts at: 1203 4008 4306 6748 7725 1203 Size: 2805 298 2442 977 1327 Fragments arranged by size: 2805 2442 1327 977 298 AgeI A'CCGG_T Cuts at: 6680 6680 Size: 7849 AhdI GACnn_n'nnGTC Cuts at: 2815 6578 2815 Size: 3763 4086 AleI CACnn'nnGTG Cuts at: 1166 3278 1166 Size: 2112 5737 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2990 3053 4082 1184 Size: 1806 63 1029 4951 Fragments arranged by size: 4951 1806 1029 63 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3711 3743 3711 Size: 32 7817 AlwNI CAG_nnn'CTG Cuts at: 3625 4285 4705 4876 5592 7164 3625 Size: 660 420 171 716 1572 4310 Fragments arranged by size: 4310 1572 716 660 420 171 ApaLI G'TGCA_C Cuts at: 5906 7062 5906 Size: 1156 6693 ApoI r'AATT_y Cuts at: 1493 3539 3589 4413 5158 5384 5536 7645 1493 Size: 2046 50 824 745 226 152 2109 1697 Fragments arranged by size: 2109 2046 1697 824 745 226 152 50 AseI AT'TA_AT Cuts at: 160 1032 5142 6403 160 Size: 872 4110 1261 1606 Fragments arranged by size: 4110 1606 1261 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7849 AvaI C'yCGr_G Cuts at: 1870 1943 5174 1870 Size: 73 3231 4545 Fragments arranged by size: 4545 3231 73 BamHI G'GATC_C Cuts at: 5179 5546 5179 Size: 367 7482 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2204 4188 4365 5170 6625 Size: 325 217 627 417 1984 177 805 1455 Cuts at: 6625 618 Size: 1842 Fragments arranged by size: 1984 1842 1455 805 627 417 325 217 177 BanII G_rGCy'C Cuts at: 1391 2196 2638 2877 5168 1391 Size: 805 442 239 2291 4072 Fragments arranged by size: 4072 2291 805 442 239 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2922 3510 4398 928 Size: 971 1023 588 888 4379 Fragments arranged by size: 4379 1023 971 888 588 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6040 6074 1903 Size: 34 4103 34 3678 Fragments arranged by size: 4103 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5741 6951 5741 Size: 1210 6639 BclI T'GATC_A Cuts at: 1548 2997 1548 Size: 1449 6400 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2246 6460 136 Size: 107 122 71 631 605 574 4214 1525 Fragments arranged by size: 4214 1525 631 605 574 122 107 71 BglII A'GATC_T Cuts at: 3421 7844 3421 Size: 4423 3426 BlpI GC'TnA_GC Cuts at: 2963 4019 5266 2963 Size: 1056 1247 5546 Fragments arranged by size: 5546 1247 1056 Bme1580I G_kGCm'C Cuts at: 1790 5910 7066 1790 Size: 4120 1156 2573 Fragments arranged by size: 4120 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2014 1318 Size: 696 7153 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5580 6538 442 Size: 416 53 83 399 24 4163 958 1753 Fragments arranged by size: 4163 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7849 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4209 4710 5035 4209 Size: 501 325 7023 Fragments arranged by size: 7023 501 325 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2450 2921 2941 4009 4508 4547 4898 6509 Size: 1211 471 20 1068 499 39 351 1611 Cuts at: 6509 1239 Size: 2579 Fragments arranged by size: 2579 1611 1211 1068 499 471 351 39 20 Bpu10I CC'TnA_GC Cuts at: 4619 4619 Size: 7849 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3163 3446 3925 4987 5005 5974 6839 7137 3163 Size: 283 479 1062 18 969 865 298 3875 Fragments arranged by size: 3875 1062 969 865 479 298 283 18 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3126 6512 882 Size: 617 1627 3386 2219 Fragments arranged by size: 3386 2219 1627 617 BsaAI yAC'GTr Cuts at: 493 1204 3610 5640 493 Size: 711 2406 2030 2702 Fragments arranged by size: 2702 2406 2030 711 BsaBI GATnn'nnATC Cuts at: 7843 7843 Size: 7849 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5656 6038 6673 275 Size: 53 83 186 936 4123 382 635 1451 Fragments arranged by size: 4123 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3288 6282 6680 6954 7101 7512 3288 Size: 2994 398 274 147 411 3625 Fragments arranged by size: 3625 2994 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2090 2120 3123 3153 4520 Size: 30 165 30 687 30 1003 30 1367 Cuts at: 4520 4550 1178 Size: 30 4477 Fragments arranged by size: 4477 1367 1003 687 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2044 2171 2576 2885 3140 5085 1186 Size: 858 127 405 309 255 1945 3950 Fragments arranged by size: 3950 1945 858 405 309 255 127 BseYI C'CCAG_C Cuts at: 2244 4521 7052 7548 2244 Size: 2277 2531 496 2545 Fragments arranged by size: 2545 2531 2277 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7276 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3714 4179 5299 6060 7088 1540 Size: 275 186 1713 465 1120 761 1028 2301 Fragments arranged by size: 2301 1713 1120 1028 761 465 275 186 BsiHKAI G_wGCw'C Cuts at: 2196 2638 2877 5910 5995 7066 2196 Size: 442 239 3033 85 1071 2979 Fragments arranged by size: 3033 2979 1071 442 239 85 BsmI GAATG_Cn' Cuts at: 4675 4981 5360 5453 4675 Size: 306 379 93 7071 Fragments arranged by size: 7071 379 306 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2196 2638 2877 5168 5910 5995 7066 Size: 399 406 442 239 2291 742 85 1071 Cuts at: 7066 1391 Size: 2174 Fragments arranged by size: 2291 2174 1071 742 442 406 399 239 85 BspEI T'CCGG_A Cuts at: 7512 7512 Size: 7849 BspHI T'CATG_A Cuts at: 3000 4002 5738 7468 3000 Size: 1002 1736 1730 3381 Fragments arranged by size: 3381 1736 1730 1002 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3485 5204 844 Size: 2641 1719 3489 Fragments arranged by size: 3489 2641 1719 BsrBI CCG'CTC Cuts at: 1394 1823 5736 1394 Size: 429 3913 3507 Fragments arranged by size: 3913 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2734 4066 6344 6518 65 Size: 1183 1486 1332 2278 174 1396 Fragments arranged by size: 2278 1486 1396 1332 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6493 6680 1700 Size: 110 4683 187 2869 Fragments arranged by size: 4683 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2133 2548 4862 96 Size: 2037 415 2314 3083 Fragments arranged by size: 3083 2314 2037 415 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7849 BssSI C'ACGA_G Cuts at: 2558 2813 3524 5909 6921 2558 Size: 255 711 2385 1012 3486 Fragments arranged by size: 3486 2385 1012 711 255 BstAPI GCAn_nnn'nTGC Cuts at: 794 2932 794 Size: 2138 5711 BstBI TT'CG_AA Cuts at: 2309 6653 2309 Size: 4344 3505 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7849 BstXI CCAn_nnnn'nTGG Cuts at: 2620 2620 Size: 7849 BstYI r'GATC_y Cuts at: 1972 2801 3339 3421 4355 4872 5179 5546 5931 Size: 829 538 82 934 517 307 367 385 Cuts at: 5931 5948 7400 7486 7498 7844 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 934 829 538 517 385 367 346 307 86 82 17 12 Bsu36I CC'TnA_GG Cuts at: 4220 4220 Size: 7849 BtgI C'CryG_G Cuts at: 513 1065 2619 3153 3714 4920 513 Size: 552 1554 534 561 1206 3442 Fragments arranged by size: 3442 1554 1206 561 552 534 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7110 Fragments arranged by size: 7110 683 56 BtsI GCAGTG_nn' Cuts at: 3820 4382 5365 6159 6179 3820 Size: 562 983 794 20 5490 Fragments arranged by size: 5490 983 794 562 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7849 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7814 DraI TTT'AAA Cuts at: 5153 5153 Size: 7849 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3512 6856 809 Size: 507 2196 3344 1802 Fragments arranged by size: 3344 2196 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2622 2661 3375 Size: 54 1425 50 275 18 792 39 714 Cuts at: 3375 3711 4176 4401 5296 6185 7814 8 Size: 336 465 225 895 889 1629 43 Fragments arranged by size: 1629 1425 895 889 792 714 465 336 275 225 54 50 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 3711 4176 5296 1537 Size: 275 1899 465 1120 4090 Fragments arranged by size: 4090 1899 1120 465 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3176 5027 5034 5779 1353 Size: 1823 1851 7 745 3423 Fragments arranged by size: 3423 1851 1823 745 7 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2067 6436 6810 6956 387 Size: 1064 138 478 4369 374 146 1280 Fragments arranged by size: 4369 1280 1064 478 374 146 138 EcoICRI GAG'CTC Cuts at: 2194 2636 2875 2194 Size: 442 239 7168 Fragments arranged by size: 7168 442 239 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2450 2921 2941 3971 4009 4065 4508 Size: 393 818 471 20 1030 38 56 443 Cuts at: 4508 4547 4832 4868 4898 5912 6509 7296 7564 Size: 39 285 36 30 1014 597 787 268 Cuts at: 7564 1239 Size: 1524 Fragments arranged by size: 1524 1030 1014 818 787 597 471 443 393 285 268 56 39 38 36 30 20 EcoNI CCTnn'n_nnAGG Cuts at: 4591 4591 Size: 7849 EcoO109I rG'GnC_Cy Cuts at: 2809 2905 3187 3820 4151 4843 2809 Size: 96 282 633 331 692 5815 Fragments arranged by size: 5815 692 633 331 282 96 EcoRI G'AATT_C Cuts at: 5158 5536 5158 Size: 378 7471 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2197 3440 4741 2197 Size: 1243 1301 5305 Fragments arranged by size: 5305 1301 1243 FspI TGC'GCA Cuts at: 6355 6355 Size: 7849 HaeII r_GCGC'y Cuts at: 1678 2716 3625 4052 6996 1678 Size: 1038 909 427 2944 2531 Fragments arranged by size: 2944 2531 1038 909 427 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2801 2833 2864 2896 3123 Size: 32 135 32 1229 32 31 32 227 Cuts at: 3123 3155 3619 3651 4076 4108 4520 4552 4739 Size: 32 464 32 425 32 412 32 187 Cuts at: 4739 4771 5028 5060 5117 5149 6490 6522 6564 Size: 32 257 32 57 32 1341 32 42 Cuts at: 6564 6596 1373 Size: 32 2626 Fragments arranged by size: 2626 1341 1229 464 425 412 257 227 187 135 57 42 32 32 32 32 32 32 32 32 32 32 32 32 32 31 HincII GTy'rAC Cuts at: 669 1657 1938 5193 5439 669 Size: 988 281 3255 246 3079 Fragments arranged by size: 3255 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 4334 748 Size: 3586 4263 HpaI GTT'AAC Cuts at: 5439 5439 Size: 7849 KpnI G_GTAC'C Cuts at: 5174 5174 Size: 7849 MfeI C'AATT_G Cuts at: 5448 5448 Size: 7849 MluI A'CGCG_T Cuts at: 7725 7725 Size: 7849 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4368 6963 7147 1305 Size: 3063 2595 184 2007 Fragments arranged by size: 3063 2595 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2624 2663 4403 10 Size: 54 1425 343 792 39 1740 3456 Fragments arranged by size: 3456 1740 1425 792 343 54 39 MslI CAynn'nnrTG Cuts at: 518 1166 3259 3278 3730 3792 4007 5807 6166 Size: 648 2093 19 452 62 215 1800 359 Cuts at: 6166 6325 518 Size: 159 2042 Fragments arranged by size: 2093 2042 1800 648 452 359 215 159 62 19 MspA1I CmG'CkG Cuts at: 1606 2244 4525 5265 5942 7090 7335 1606 Size: 638 2281 740 677 1148 245 2120 Fragments arranged by size: 2281 2120 1148 740 677 638 245 NcoI C'CATG_G Cuts at: 513 1065 3153 4920 513 Size: 552 2088 1767 3442 Fragments arranged by size: 3442 2088 1767 552 NdeI CA'TA_TG Cuts at: 387 2495 387 Size: 2108 5741 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7849 NotI GC'GGCC_GC Cuts at: 4176 5296 4176 Size: 1120 6729 NspI r_CATG'y Cuts at: 1132 1229 3030 3391 4310 5207 6752 1132 Size: 97 1801 361 919 897 1545 2229 Fragments arranged by size: 2229 1801 1545 919 897 361 97 PasI CC'CwG_GG Cuts at: 1348 2599 1348 Size: 1251 6598 PciI A'CATG_T Cuts at: 4306 6748 4306 Size: 2442 5407 PflMI CCAn_nnn'nTGG Cuts at: 1108 2220 4921 1108 Size: 1112 2701 4036 Fragments arranged by size: 4036 2701 1112 PfoI T'CCnGG_A Cuts at: 3852 4875 3852 Size: 1023 6826 PmeI GTTT'AAAC Cuts at: 5153 5153 Size: 7849 PmlI CAC'GTG Cuts at: 3610 3610 Size: 7849 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5918 5950 7431 7463 5918 Size: 32 1481 32 6304 Fragments arranged by size: 6304 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2809 2905 3187 3820 2809 Size: 96 282 633 6838 Fragments arranged by size: 6838 633 282 96 PshAI GACnn'nnGTC Cuts at: 1613 3452 1613 Size: 1839 6010 PsiI TTA'TAA Cuts at: 5419 5419 Size: 7849 PstI C_TGCA'G Cuts at: 830 4377 4701 5201 830 Size: 3547 324 500 3478 Fragments arranged by size: 3547 3478 500 324 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7849 PvuII CAG'CTG Cuts at: 2244 4525 2244 Size: 2281 5568 SacI G_AGCT'C Cuts at: 2196 2638 2877 2196 Size: 442 239 7168 Fragments arranged by size: 7168 442 239 SalI G'TCGA_C Cuts at: 1936 5191 1936 Size: 3255 4594 SanDI GG'GwC_CC Cuts at: 3187 3820 3187 Size: 633 7216 SbfI CC_TGCA'GG Cuts at: 5201 5201 Size: 7849 ScaI AGT'ACT Cuts at: 2200 2567 6097 7809 2200 Size: 367 3530 1712 2240 Fragments arranged by size: 3530 2240 1712 367 SexAI A'CCwGG_T Cuts at: 2226 2226 Size: 7849 SfcI C'TryA_G Cuts at: 826 1045 4373 4697 5197 6332 7013 7204 826 Size: 219 3328 324 500 1135 681 191 1471 Fragments arranged by size: 3328 1471 1135 681 500 324 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7849 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7849 SmaI CCC'GGG Cuts at: 1872 5176 1872 Size: 3304 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3142 3425 3904 4966 5020 5953 Size: 197 926 1199 283 479 1062 54 933 Cuts at: 5953 6854 7116 820 Size: 901 262 1553 Fragments arranged by size: 1553 1199 1062 933 926 901 479 283 262 197 54 SnaBI TAC'GTA Cuts at: 493 5640 493 Size: 5147 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7849 SphI G_CATG'C Cuts at: 1132 5207 1132 Size: 4075 3774 SspI AAT'ATT Cuts at: 5 52 3965 5773 5 Size: 47 3913 1808 2081 Fragments arranged by size: 3913 2081 1808 47 StuI AGG'CCT Cuts at: 1517 3701 5015 1517 Size: 2184 1314 4351 Fragments arranged by size: 4351 2184 1314 StyI C'CwwG_G Cuts at: 513 1065 2385 2542 3153 3466 4920 5288 513 Size: 552 1320 157 611 313 1454 368 3074 Fragments arranged by size: 3074 1454 1320 611 552 368 313 157 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3394 5115 5876 5893 6046 6231 6676 Size: 1255 1814 1721 761 17 153 185 445 Cuts at: 6676 325 Size: 1498 Fragments arranged by size: 1814 1721 1498 1255 761 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2133 2198 2548 Size: 274 80 33 51 1443 156 65 350 Cuts at: 2548 2565 4862 6095 7807 96 Size: 17 2297 1233 1712 138 Fragments arranged by size: 2297 1712 1443 1233 350 274 156 138 80 65 51 33 17 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2023 2055 3350 3382 7809 7841 2023 Size: 32 1295 32 4427 32 2031 Fragments arranged by size: 4427 2031 1295 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2228 3137 1316 Size: 912 909 6028 Fragments arranged by size: 6028 912 909 XbaI T'CTAG_A Cuts at: 5185 5185 Size: 7849 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3110 4144 3110 Size: 1034 6815 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7849 XmaI C'CCGG_G Cuts at: 1870 5174 1870 Size: 3304 4545 XmnI GAAnn'nnTTC Cuts at: 5978 5978 Size: 7849 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5657 276 Size: 53 83 186 936 4123 2468 Fragments arranged by size: 4123 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI