################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp718I AspEI BamHI BcgI BciVI BfrI BglII BsaBI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI Eam1105I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI HindIII HinfI HpaI KpnI MamI MfeI MroI MseI MunI NcoI NdeI NheI NruI NsiI NspV PacI PleI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SbfI SexAI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I XbaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AgeI 1150 ACCGGT A^CCGGT AlwI 354 GATCC GGATC(4/5) Asp700I 1566 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1681 GAGCAC G[AT]GC[AT]^C AspI 1414 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1386 CCTAGG C^CTAGG BbsI 1521 GAAGAC GAAGAC(2/6) BbvCI 2049 CCTCAGC CCTCAGC(-5/-2) BclI 89 TGATCA T^GATCA BlnI 1386 CCTAGG C^CTAGG BpuAI 1521 GAAGAC GAAGAC(2/6) BsaI 1147 GAGACC GGTCTC(1/5) BsaWI 1150 ACCGGT [AT]^CCGG[AT] BsiHKAI 1681 GAGCAC G[AT]GC[AT]^C BsmBI 1035 CGTCTC CGTCTC(1/5) BsmI 1259 GCATTC GAATGC(1/-1) BssHII 119 GCGCGC G^CGCGC BstYI 353 AGATCC [GA]^GATC[CT] DrdI 1027 GACGCGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1396 CCTTTTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 89 TGATCA T^GATCA HincII 574 GTCAAC GT[CT]^[GA]AC HindII 574 GTCAAC GT[CT]^[GA]AC MaeIII 1241 GTGAC ^GT[ACGT]AC MflI 353 AGATCC [GA]^GATC[CT] PaeR7I 1189 CTCGAG C^TCGAG PflFI 1414 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 1150 ACCGGT A^CCGGT ScaI 404 AGTACT AGT^ACT SphI 1649 GCATGC GCATG^C StuI 500 AGGCCT AGG^CCT Tsp45I 1241 GTGAC ^GT[GC]AC Tth111I 1414 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1994 CCAAGGTGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1189 CTCGAG C^TCGAG XhoII 353 AGATCC [GA]^GATC[CT] XmnI 1566 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AlwNI 37 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 826 CAGAAGCTG Aor51HI 1004 AGCGCT AGC^GCT 1335 AGCGCT AviII 1604 TGCGCA TGC^GCA 1688 TGCGCA BalI 1182 TGGCCA TGG^CCA 2024 TGGCCA BbrPI 1306 CACGTG CAC^GTG 1318 CACGTG BfaI 917 CTAG C^TAG 1387 CTAG BsgI 1984 CTGCAC GTGCAG(16/14) 100 GTGCAG BsrBI 1054 CCGCTC CCGCTC(-3/-3) 709 GAGCGG DpnII 90 GATC ^GATC 354 GATC DraIII 626 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG 1780 CACGCCGTG Eco47III 1004 AGCGCT AGC^GCT 1335 AGCGCT FspI 1604 TGCGCA TGC^GCA 1688 TGCGCA MaeI 917 CTAG C^TAG 1387 CTAG MboI 90 GATC ^GATC 354 GATC MluI 599 ACGCGT A^CGCGT 1640 ACGCGT MluNI 1182 TGGCCA TGG^CCA 2024 TGGCCA MscI 1182 TGGCCA TGG^CCA 2024 TGGCCA MslI 93 CATCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1770 CATGAAGATG NdeII 90 GATC ^GATC 354 GATC PflMI 198 CCATGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 825 CCAGAAGCTGG PmaCI 1306 CACGTG CAC^GTG 1318 CACGTG PmlI 1306 CACGTG CAC^GTG 1318 CACGTG PstI 46 CTGCAG CTGCA^G 349 CTGCAG SapI 681 GAAGAGC GCTCTTC(1/4) 1012 GAAGAGC Sau3AI 90 GATC ^GATC 354 GATC SfaNI 1776 GATGC GCATC(5/9) 1691 GCATC SfcI 46 CTGCAG C^T[GA][CT]AG 349 CTGCAG TspRI 583 CACTG CA[GC]TG[ACGT][ACGT]^ 1528 CACTG Van91I 198 CCATGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 825 CCAGAAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI ApaI AvaI AvaII BanI BanII BbeI BbvI BcnI BglI BlpI BmyI BpmI Bpu1102I BsaAI BsaHI BsaJI BseRI BsiEI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DraII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI KasI Ksp632I KspI MaeII MboII MnlI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NgoMIV NlaIII NlaIV NotI NspI PpuMI PvuII RsaI SacII Sau96I ScrFI SfiI SfoI SmaI SmlI StyI TaqI TseI TthHB8I XmaCI XmaI