(Circular) (Six-base) MAPSORT of: pFN21AB9759.seq Check: 2396 from: 1 to: 6847 pFN21AB9759 6847 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2427 2427 Size: 6847 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4657 278 Size: 53 83 186 936 3121 2468 Fragments arranged by size: 3121 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4190 1937 Size: 2253 4594 Acc65I G'GTAC_C Cuts at: 4168 4168 Size: 6847 AclI AA'CG_TT Cuts at: 1563 4974 5347 1563 Size: 3411 373 3063 Fragments arranged by size: 3411 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3953 4910 6294 6562 1632 Size: 2321 957 1384 268 1917 Fragments arranged by size: 2321 1917 1384 957 268 AfeI AGC'GCT Cuts at: 3011 3342 3011 Size: 331 6516 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6650 AflIII A'CryG_T Cuts at: 1203 2604 3258 3645 5746 6723 1203 Size: 1401 654 387 2101 977 1327 Fragments arranged by size: 2101 1401 1327 977 654 387 AgeI A'CCGG_T Cuts at: 3155 5678 3155 Size: 2523 4324 AhdI GACnn_n'nnGTC Cuts at: 5576 5576 Size: 6847 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6847 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6847 AlwNI CAG_nnn'CTG Cuts at: 2047 2836 4590 6162 2047 Size: 789 1754 1572 2732 Fragments arranged by size: 2732 1754 1572 789 ApaI G_GGCC'C Cuts at: 2567 3057 3108 3399 4113 2567 Size: 490 51 291 714 5301 Fragments arranged by size: 5301 714 490 291 51 ApaLI G'TGCA_C Cuts at: 4904 6060 4904 Size: 1156 5691 ApoI r'AATT_y Cuts at: 1493 4156 4382 4534 6643 1493 Size: 2663 226 152 2109 1697 Fragments arranged by size: 2663 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5401 160 Size: 872 4369 1606 Fragments arranged by size: 4369 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6847 AvaI C'yCGr_G Cuts at: 1870 1943 2305 2560 2887 2894 3047 3194 3233 Size: 73 362 255 327 7 153 147 39 Cuts at: 3233 3703 3884 4172 1870 Size: 470 181 288 4545 Fragments arranged by size: 4545 470 362 327 288 255 181 153 147 73 39 7 AvrII C'CTAG_G Cuts at: 3391 3391 Size: 6847 BamHI G'GATC_C Cuts at: 4177 4544 4177 Size: 367 6480 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2023 2327 2707 3070 4168 Size: 325 217 627 236 304 380 363 1098 Cuts at: 4168 5623 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1098 627 380 363 325 304 236 217 BanII G_rGCy'C Cuts at: 1391 2219 2567 2693 2702 2901 3057 3108 3234 Size: 828 348 126 9 199 156 51 126 Cuts at: 3234 3399 3436 3840 4113 4166 1391 Size: 165 37 404 273 53 4072 Fragments arranged by size: 4072 828 404 348 273 199 165 156 126 126 53 51 37 9 BbeI G_GCGC'C Cuts at: 2027 2331 2711 3074 2027 Size: 304 380 363 5800 Fragments arranged by size: 5800 380 363 304 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3533 928 Size: 971 1634 4242 Fragments arranged by size: 4242 1634 971 BbvCI CC'TCA_GC Cuts at: 4055 4055 Size: 6847 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5038 5072 1903 Size: 34 50 34 3017 34 3678 Fragments arranged by size: 3678 3017 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4739 5949 4739 Size: 1210 5637 BclI T'GATC_A Cuts at: 1548 2094 1548 Size: 546 6301 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2309 2336 2697 Size: 107 122 71 631 605 637 27 361 Cuts at: 2697 2716 3052 3362 3371 3380 3621 3809 3861 Size: 19 336 310 9 9 241 188 52 Cuts at: 3861 5458 136 Size: 1597 1525 Fragments arranged by size: 1597 1525 637 631 605 361 336 310 241 188 122 107 71 52 27 19 9 9 BglII A'GATC_T Cuts at: 6842 6842 Size: 6847 BlpI GC'TnA_GC Cuts at: 2313 2727 3926 4082 4264 2313 Size: 414 1199 156 182 4896 Fragments arranged by size: 4896 1199 414 182 156 Bme1580I G_kGCm'C Cuts at: 1790 2567 3057 3108 3399 4113 4908 6064 1790 Size: 777 490 51 291 714 795 1156 2573 Fragments arranged by size: 2573 1156 795 777 714 490 291 51 BmgBI CAC'GTC Cuts at: 1318 2092 1318 Size: 774 6073 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2516 4578 5536 Size: 416 53 83 399 24 1099 2062 958 Cuts at: 5536 442 Size: 1753 Fragments arranged by size: 2062 1753 1099 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6847 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2575 3013 2575 Size: 438 6409 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2020 2858 4031 5507 1239 Size: 781 838 1173 1476 2579 Fragments arranged by size: 2579 1476 1173 838 781 Bpu10I CC'TnA_GC Cuts at: 4055 4055 Size: 6847 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2147 2378 3611 4972 5837 6135 2147 Size: 231 1233 1361 865 298 2859 Fragments arranged by size: 2859 1361 1233 865 298 231 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3146 5510 882 Size: 617 1647 2364 2219 Fragments arranged by size: 2364 2219 1647 617 BsaAI yAC'GTr Cuts at: 493 1204 3259 3313 3325 4638 493 Size: 711 2055 54 12 1313 2702 Fragments arranged by size: 2702 2055 1313 711 54 12 BsaBI GATnn'nnATC Cuts at: 6841 6841 Size: 6847 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2024 2328 2708 3071 Size: 53 83 186 936 491 304 380 363 Cuts at: 3071 3846 3891 4654 5036 5671 275 Size: 775 45 763 382 635 1451 Fragments arranged by size: 1451 936 775 763 635 491 382 380 363 304 186 83 53 45 BsaWI w'CCGG_w Cuts at: 3155 5280 5678 5952 6099 6510 3155 Size: 2125 398 274 147 411 3492 Fragments arranged by size: 3492 2125 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6622 Fragments arranged by size: 6622 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2855 2918 3164 3344 3347 1186 Size: 1669 63 246 180 3 4686 Fragments arranged by size: 4686 1669 246 180 63 3 BseYI C'CCAG_C Cuts at: 2762 2980 3002 3173 3287 3377 4048 6050 6546 Size: 218 22 171 114 90 671 2002 496 Cuts at: 6546 2762 Size: 3063 Fragments arranged by size: 3063 2002 671 496 218 171 114 90 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2126 3974 1163 Size: 573 390 1848 4036 Fragments arranged by size: 4036 1848 573 390 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2223 2260 2292 2298 2468 2625 Size: 275 186 222 37 32 6 170 157 Cuts at: 2625 2720 2908 3039 3366 3804 3813 3918 3954 Size: 95 188 131 327 438 9 105 36 Cuts at: 3954 3960 4044 4074 4107 4120 4132 4297 5058 Size: 6 84 30 33 13 12 165 761 Cuts at: 5058 6086 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 438 327 275 222 188 186 170 165 157 131 105 95 84 37 36 33 32 30 13 12 9 6 6 BsiHKAI G_wGCw'C Cuts at: 3690 4908 4993 6064 3690 Size: 1218 85 1071 4473 Fragments arranged by size: 4473 1218 1071 85 BsmI GAATG_Cn' Cuts at: 3264 4358 4451 3264 Size: 1094 93 5660 Fragments arranged by size: 5660 1094 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3046 3046 Size: 6847 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2219 2567 2693 2702 2901 3057 3108 Size: 399 429 348 126 9 199 156 51 Cuts at: 3108 3234 3399 3436 3690 3840 4113 4166 4908 Size: 126 165 37 254 150 273 53 742 Cuts at: 4908 4993 6064 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 429 399 348 273 254 199 165 156 150 126 126 85 53 51 37 9 BspEI T'CCGG_A Cuts at: 6510 6510 Size: 6847 BspHI T'CATG_A Cuts at: 4736 6466 4736 Size: 1730 5117 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2196 2361 2427 4202 844 Size: 1352 165 66 1775 3489 Fragments arranged by size: 3489 1775 1352 165 66 BsrBI CCG'CTC Cuts at: 1394 1823 2716 3061 4734 1394 Size: 429 893 345 1673 3507 Fragments arranged by size: 3507 1673 893 429 345 BsrDI GCAATG_nn' Cuts at: 65 1248 5342 5516 65 Size: 1183 4094 174 1396 Fragments arranged by size: 4094 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2255 2494 2768 3155 3386 3803 5491 Size: 110 445 239 274 387 231 417 1688 Cuts at: 5491 5678 1700 Size: 187 2869 Fragments arranged by size: 2869 1688 445 417 387 274 239 231 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6847 BssHII G'CGCG_C Cuts at: 1925 2124 1925 Size: 199 6648 BssSI C'ACGA_G Cuts at: 2369 2948 3521 4907 5919 2369 Size: 579 573 1386 1012 3297 Fragments arranged by size: 3297 1386 1012 579 573 BstAPI GCAn_nnn'nTGC Cuts at: 794 2047 2412 2636 3270 3564 3988 794 Size: 1253 365 224 634 294 424 3653 Fragments arranged by size: 3653 1253 634 424 365 294 224 BstBI TT'CG_AA Cuts at: 5651 5651 Size: 6847 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6847 BstYI r'GATC_y Cuts at: 1972 2358 4177 4544 4929 4946 6398 6484 6496 Size: 386 1819 367 385 17 1452 86 12 Cuts at: 6496 6842 1972 Size: 346 1977 Fragments arranged by size: 1977 1819 1452 386 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2292 2298 2401 2547 2574 2703 3789 Size: 552 1227 6 103 146 27 129 1086 Cuts at: 3789 3954 513 Size: 165 3406 Fragments arranged by size: 3406 1227 1086 552 165 146 129 103 27 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6108 Fragments arranged by size: 6108 683 56 BtsI GCAGTG_nn' Cuts at: 2587 4363 5157 5177 2587 Size: 1776 794 20 4257 Fragments arranged by size: 4257 1776 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6847 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6812 DraI TTT'AAA Cuts at: 4151 4151 Size: 6847 DraIII CAC_nnn'GTG Cuts at: 2636 3790 2636 Size: 1154 5693 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3038 5854 809 Size: 507 1722 2816 1802 Fragments arranged by size: 2816 1802 1722 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2029 2145 2220 Size: 54 1425 50 275 18 199 116 75 Cuts at: 2220 2257 2289 2295 2337 2465 2622 2717 2905 Size: 37 32 6 42 128 157 95 188 Cuts at: 2905 3036 3187 3363 3372 3504 3801 3810 3915 Size: 131 151 176 9 132 297 9 105 Cuts at: 3915 3951 3957 4029 4041 4071 4104 4117 4294 Size: 36 6 72 12 30 33 13 177 Cuts at: 4294 5183 6812 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 297 275 199 188 177 176 157 151 132 131 128 116 105 95 75 72 54 50 43 42 37 36 33 32 30 18 13 12 9 9 6 6 EagI C'GGCC_G Cuts at: 1537 1812 2220 2257 2289 2295 2465 2622 2717 Size: 275 408 37 32 6 170 157 95 Cuts at: 2717 2905 3036 3363 3801 3810 3915 3951 3957 Size: 188 131 327 438 9 105 36 6 Cuts at: 3957 4041 4071 4104 4117 4294 1537 Size: 84 30 33 13 177 4090 Fragments arranged by size: 4090 438 408 327 275 188 177 170 157 131 105 95 84 37 36 33 32 30 13 9 6 6 EarI CTCTTCn'nnn_ Cuts at: 1353 2123 2681 3012 4777 1353 Size: 770 558 331 1765 3423 Fragments arranged by size: 3423 1765 770 558 331 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2219 2523 5434 5808 5954 387 Size: 1064 138 630 304 2911 374 146 1280 Fragments arranged by size: 2911 1280 1064 630 374 304 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2020 2858 3953 4031 4910 5507 6294 Size: 393 388 838 1095 78 879 597 787 Cuts at: 6294 6562 1239 Size: 268 1524 Fragments arranged by size: 1524 1095 879 838 787 597 393 388 268 78 EcoNI CCTnn'n_nnAGG Cuts at: 3405 3405 Size: 6847 EcoO109I rG'GnC_Cy Cuts at: 2476 2612 2891 3053 3054 3169 3395 3396 3499 Size: 136 279 162 1 115 226 1 103 Cuts at: 3499 2476 Size: 5824 Fragments arranged by size: 5824 279 226 162 136 115 103 1 1 EcoRI G'AATT_C Cuts at: 4156 4534 4156 Size: 378 6469 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2098 3895 2098 Size: 1797 5050 FspI TGC'GCA Cuts at: 3611 3695 5353 3611 Size: 84 1658 5105 Fragments arranged by size: 5105 1658 84 FspAI rTGC'GCAy Cuts at: 3695 3695 Size: 6847 HaeII r_GCGC'y Cuts at: 1678 2027 2157 2331 2378 2401 2650 2711 2769 Size: 349 130 174 47 23 249 61 58 Cuts at: 2769 3013 3074 3344 3821 4010 4095 5994 1678 Size: 244 61 270 477 189 85 1899 2531 Fragments arranged by size: 2531 1899 477 349 270 249 244 189 174 130 85 61 61 58 47 23 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4121 4153 5488 5520 5562 Size: 32 135 32 2549 32 1335 32 42 Cuts at: 5562 5594 1373 Size: 32 2626 Fragments arranged by size: 2626 2549 1335 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2581 4191 4437 669 Size: 988 281 643 1610 246 3079 Fragments arranged by size: 3079 1610 988 643 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6847 HpaI GTT'AAC Cuts at: 4437 4437 Size: 6847 KasI G'GCGC_C Cuts at: 2023 2327 2707 3070 2023 Size: 304 380 363 5800 Fragments arranged by size: 5800 380 363 304 KpnI G_GTAC'C Cuts at: 4172 4172 Size: 6847 MfeI C'AATT_G Cuts at: 4446 4446 Size: 6847 MluI A'CGCG_T Cuts at: 2604 3645 6723 2604 Size: 1041 3078 2728 Fragments arranged by size: 3078 2728 1041 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2883 5961 6145 1305 Size: 1578 3078 184 2007 Fragments arranged by size: 3078 2007 1578 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3189 4031 10 Size: 54 1425 343 1357 842 2826 Fragments arranged by size: 2826 1425 1357 842 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2102 3779 4805 5164 5323 518 Size: 648 936 1677 1026 359 159 2042 Fragments arranged by size: 2042 1677 1026 936 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2044 2294 2549 2576 2652 2705 2722 2821 Size: 438 250 255 27 76 53 17 99 Cuts at: 2821 2880 3301 3377 3471 3925 3956 4027 4263 Size: 59 421 76 94 454 31 71 236 Cuts at: 4263 4940 6088 6333 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 454 438 421 255 250 245 236 99 94 76 76 71 59 53 31 27 17 NaeI GCC'GGC Cuts at: 2770 3388 3805 2770 Size: 618 417 5812 Fragments arranged by size: 5812 618 417 NarI GG'CG_CC Cuts at: 2024 2328 2708 3071 2024 Size: 304 380 363 5800 Fragments arranged by size: 5800 380 363 304 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6295 NdeI CA'TA_TG Cuts at: 387 387 Size: 6847 NgoMIV G'CCGG_C Cuts at: 2768 3386 3803 2768 Size: 618 417 5812 Fragments arranged by size: 5812 618 417 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6847 NotI GC'GGCC_GC Cuts at: 2289 2717 3951 4294 2289 Size: 428 1234 343 4842 Fragments arranged by size: 4842 1234 428 343 NspI r_CATG'y Cuts at: 1132 1229 3610 3658 3694 4205 5750 1132 Size: 97 2381 48 36 511 1545 2229 Fragments arranged by size: 2381 2229 1545 511 97 48 36 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6847 PciI A'CATG_T Cuts at: 5746 5746 Size: 6847 PflMI CCAn_nnn'nTGG Cuts at: 1108 2209 2836 1108 Size: 1101 627 5119 Fragments arranged by size: 5119 1101 627 PmeI GTTT'AAAC Cuts at: 4151 4151 Size: 6847 PmlI CAC'GTG Cuts at: 3313 3325 3313 Size: 12 6835 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4916 4948 6429 6461 4916 Size: 32 1481 32 5302 Fragments arranged by size: 5302 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2612 2891 3169 3499 2612 Size: 279 278 330 5960 Fragments arranged by size: 5960 330 279 278 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6847 PsiI TTA'TAA Cuts at: 4417 4417 Size: 6847 PspOMI G'GGCC_C Cuts at: 2563 3053 3104 3395 4109 2563 Size: 490 51 291 714 5301 Fragments arranged by size: 5301 714 490 291 51 PspXI vC'TCGA_Gb Cuts at: 3194 3194 Size: 6847 PstI C_TGCA'G Cuts at: 830 2055 2358 4199 830 Size: 1225 303 1841 3478 Fragments arranged by size: 3478 1841 1225 303 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6847 PvuII CAG'CTG Cuts at: 2044 3301 3925 2044 Size: 1257 624 4966 Fragments arranged by size: 4966 1257 624 SacII CC_GC'GG Cuts at: 2295 2550 2577 2706 3957 2295 Size: 255 27 129 1251 5185 Fragments arranged by size: 5185 1251 255 129 27 SalI G'TCGA_C Cuts at: 1936 4189 1936 Size: 2253 4594 SanDI GG'GwC_CC Cuts at: 2612 2891 2612 Size: 279 6568 SapI GCTCTTCn'nnn_ Cuts at: 2681 3012 2681 Size: 331 6516 SbfI CC_TGCA'GG Cuts at: 4199 4199 Size: 6847 ScaI AGT'ACT Cuts at: 2411 5095 6807 2411 Size: 2684 1712 2451 Fragments arranged by size: 2684 2451 1712 SfcI C'TryA_G Cuts at: 826 1045 2051 2354 4195 5330 6011 6202 826 Size: 219 1006 303 1841 1135 681 191 1471 Fragments arranged by size: 1841 1471 1135 1006 681 303 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3362 3371 3380 3809 3362 Size: 9 9 429 6400 Fragments arranged by size: 6400 429 9 9 SfoI GGC'GCC Cuts at: 2025 2329 2709 3072 2025 Size: 304 380 363 5800 Fragments arranged by size: 5800 380 363 304 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6847 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6847 SmaI CCC'GGG Cuts at: 1872 2307 2889 3049 4174 1872 Size: 435 582 160 1125 4545 Fragments arranged by size: 4545 1125 582 435 160 SmlI C'TyrA_G Cuts at: 820 1017 1943 2162 2393 3194 3626 4951 5852 Size: 197 926 219 231 801 432 1325 901 Cuts at: 5852 6114 820 Size: 262 1553 Fragments arranged by size: 1553 1325 926 901 801 432 262 231 219 197 SnaBI TAC'GTA Cuts at: 493 4638 493 Size: 4145 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6847 SphI G_CATG'C Cuts at: 1132 3658 4205 1132 Size: 2526 547 3774 Fragments arranged by size: 3774 2526 547 SspI AAT'ATT Cuts at: 5 52 4771 5 Size: 47 4719 2081 Fragments arranged by size: 4719 2081 47 StuI AGG'CCT Cuts at: 1517 2507 1517 Size: 990 5857 StyI C'CwwG_G Cuts at: 513 1065 2073 3391 3999 4286 513 Size: 552 1008 1318 608 287 3074 Fragments arranged by size: 3074 1318 1008 608 552 287 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4874 4891 5044 5229 5674 325 Size: 1255 3294 17 153 185 445 1498 Fragments arranged by size: 3294 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2409 5093 6805 Size: 274 80 33 51 1443 432 2684 1712 Cuts at: 6805 96 Size: 138 Fragments arranged by size: 2684 1712 1443 432 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6807 6839 6807 Size: 32 6815 Tth111I GACn'n_nGTC Cuts at: 1316 3422 1316 Size: 2106 4741 XbaI T'CTAG_A Cuts at: 4183 4183 Size: 6847 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4006 4006 Size: 6847 XhoI C'TCGA_G Cuts at: 1943 3194 1943 Size: 1251 5596 XmaI C'CCGG_G Cuts at: 1870 2305 2887 3047 4172 1870 Size: 435 582 160 1125 4545 Fragments arranged by size: 4545 1125 582 435 160 XmnI GAAnn'nnTTC Cuts at: 3575 4976 3575 Size: 1401 5446 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4655 276 Size: 53 83 186 936 3121 2468 Fragments arranged by size: 3121 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI NruI NsiI PacI PfoI PsrI RsrII SacI SexAI SrfI SwaI