(Linear) (Six-base) MAPSORT of: KIBB9759.seq Check: 839 from: 1 to: 2142 KIBB9759.orf With 182 enzymes: SgfI * July 15, 2010 18:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 422 2142 Size: 422 1720 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1948 2142 Size: 1948 194 AfeI AGC'GCT Cuts at: 0 1006 1337 2142 Size: 1006 331 805 Fragments arranged by size: 1006 805 331 AflIII A'CryG_T Cuts at: 0 599 1253 1640 2142 Size: 599 654 387 502 Fragments arranged by size: 654 599 502 387 AgeI A'CCGG_T Cuts at: 0 1150 2142 Size: 1150 992 AlwNI CAG_nnn'CTG Cuts at: 0 42 831 2142 Size: 42 789 1311 Fragments arranged by size: 1311 789 42 ApaI G_GGCC'C Cuts at: 0 562 1052 1103 1394 2108 2142 Size: 562 490 51 291 714 34 Fragments arranged by size: 714 562 490 291 51 34 AvaI C'yCGr_G Cuts at: 0 300 555 882 889 1042 1189 1228 1698 Size: 300 255 327 7 153 147 39 470 Cuts at: 1698 1879 2142 Size: 181 263 Fragments arranged by size: 470 327 300 263 255 181 153 147 39 7 AvrII C'CTAG_G Cuts at: 0 1386 2142 Size: 1386 756 BanI G'GyrC_C Cuts at: 0 18 322 702 1065 2142 Size: 18 304 380 363 1077 Fragments arranged by size: 1077 380 363 304 18 BanII G_rGCy'C Cuts at: 0 214 562 688 697 896 1052 1103 1229 Size: 214 348 126 9 199 156 51 126 Cuts at: 1229 1394 1431 1835 2108 2142 Size: 165 37 404 273 34 Fragments arranged by size: 404 348 273 214 199 165 156 126 126 51 37 34 9 BbeI G_GCGC'C Cuts at: 0 22 326 706 1069 2142 Size: 22 304 380 363 1073 Fragments arranged by size: 1073 380 363 304 22 BbsI GAAGACnn'nnnn_ Cuts at: 0 1528 2142 Size: 1528 614 BbvCI CC'TCA_GC Cuts at: 0 2050 2142 Size: 2050 92 BclI T'GATC_A Cuts at: 0 89 2142 Size: 89 2053 BglI GCCn_nnn'nGGC Cuts at: 0 304 331 692 711 1047 1357 1366 1375 Size: 304 27 361 19 336 310 9 9 Cuts at: 1375 1616 1804 1856 2142 Size: 241 188 52 286 Fragments arranged by size: 361 336 310 304 286 241 188 52 27 19 9 9 BlpI GC'TnA_GC Cuts at: 0 308 722 1921 2077 2142 Size: 308 414 1199 156 65 Fragments arranged by size: 1199 414 308 156 65 Bme1580I G_kGCm'C Cuts at: 0 562 1052 1103 1394 2108 2142 Size: 562 490 51 291 714 34 Fragments arranged by size: 714 562 490 291 51 34 BmgBI CAC'GTC Cuts at: 0 87 2142 Size: 87 2055 BmrI ACTGGGnnnn_n' Cuts at: 0 511 2142 Size: 511 1631 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 570 1008 2142 Size: 570 438 1134 Fragments arranged by size: 1134 570 438 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 15 853 2026 2142 Size: 15 838 1173 116 Fragments arranged by size: 1173 838 116 15 Bpu10I CC'TnA_GC Cuts at: 0 2050 2142 Size: 2050 92 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 142 373 1606 2142 Size: 142 231 1233 536 Fragments arranged by size: 1233 536 231 142 BsaI GGTCTCn'nnnn_ Cuts at: 0 1141 2142 Size: 1141 1001 BsaAI yAC'GTr Cuts at: 0 1254 1308 1320 2142 Size: 1254 54 12 822 Fragments arranged by size: 1254 822 54 12 BsaHI Gr'CG_yC Cuts at: 0 19 323 703 1066 1841 1886 2142 Size: 19 304 380 363 775 45 256 Fragments arranged by size: 775 380 363 304 256 45 19 BsaWI w'CCGG_w Cuts at: 0 1150 2142 Size: 1150 992 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 850 913 1159 1339 1342 2142 Size: 850 63 246 180 3 800 Fragments arranged by size: 850 800 246 180 63 3 BseYI C'CCAG_C Cuts at: 0 757 975 997 1168 1282 1372 2043 2142 Size: 757 218 22 171 114 90 671 99 Fragments arranged by size: 757 671 218 171 114 99 90 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 121 1969 2142 Size: 121 1848 173 Fragments arranged by size: 1848 173 121 BsiEI CG_ry'CG Cuts at: 0 218 255 287 293 463 620 715 903 Size: 218 37 32 6 170 157 95 188 Cuts at: 903 1034 1361 1799 1808 1913 1949 1955 2039 Size: 131 327 438 9 105 36 6 84 Cuts at: 2039 2069 2102 2115 2127 2142 Size: 30 33 13 12 15 Fragments arranged by size: 438 327 218 188 170 157 131 105 95 84 37 36 33 32 30 15 13 12 9 6 6 BsiHKAI G_wGCw'C Cuts at: 0 1685 2142 Size: 1685 457 BsmI GAATG_Cn' Cuts at: 0 1259 2142 Size: 1259 883 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1041 2142 Size: 1041 1101 Bsp1286I G_dGCh'C Cuts at: 0 214 562 688 697 896 1052 1103 1229 Size: 214 348 126 9 199 156 51 126 Cuts at: 1229 1394 1431 1685 1835 2108 2142 Size: 165 37 254 150 273 34 Fragments arranged by size: 348 273 254 214 199 165 156 150 126 126 51 37 34 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 191 356 422 2142 Size: 191 165 66 1720 Fragments arranged by size: 1720 191 165 66 BsrBI CCG'CTC Cuts at: 0 711 1056 2142 Size: 711 345 1086 Fragments arranged by size: 1086 711 345 BsrFI r'CCGG_y Cuts at: 0 250 489 763 1150 1381 1798 2142 Size: 250 239 274 387 231 417 344 Fragments arranged by size: 417 387 344 274 250 239 231 BssHII G'CGCG_C Cuts at: 0 119 2142 Size: 119 2023 BssSI C'ACGA_G Cuts at: 0 364 943 1516 2142 Size: 364 579 573 626 Fragments arranged by size: 626 579 573 364 BstAPI GCAn_nnn'nTGC Cuts at: 0 42 407 631 1265 1559 1983 2142 Size: 42 365 224 634 294 424 159 Fragments arranged by size: 634 424 365 294 224 159 42 BstYI r'GATC_y Cuts at: 0 353 2142 Size: 353 1789 BtgI C'CryG_G Cuts at: 0 287 293 396 542 569 698 1784 1949 Size: 287 6 103 146 27 129 1086 165 Cuts at: 1949 2142 Size: 193 Fragments arranged by size: 1086 287 193 165 146 129 103 27 6 BtsI GCAGTG_nn' Cuts at: 0 582 2142 Size: 582 1560 DraIII CAC_nnn'GTG Cuts at: 0 631 1785 2142 Size: 631 1154 357 Fragments arranged by size: 1154 631 357 DrdI GACnn_nn'nnGTC Cuts at: 0 1033 2142 Size: 1033 1109 EaeI y'GGCC_r Cuts at: 0 24 140 215 252 284 290 332 460 Size: 24 116 75 37 32 6 42 128 Cuts at: 460 617 712 900 1031 1182 1358 1367 1499 Size: 157 95 188 131 151 176 9 132 Cuts at: 1499 1796 1805 1910 1946 1952 2024 2036 2066 Size: 297 9 105 36 6 72 12 30 Cuts at: 2066 2099 2112 2142 Size: 33 13 30 Fragments arranged by size: 297 188 176 157 151 132 131 128 116 105 95 75 72 42 37 36 33 32 30 30 24 13 12 9 9 6 6 EagI C'GGCC_G Cuts at: 0 215 252 284 290 460 617 712 900 Size: 215 37 32 6 170 157 95 188 Cuts at: 900 1031 1358 1796 1805 1910 1946 1952 2036 Size: 131 327 438 9 105 36 6 84 Cuts at: 2036 2066 2099 2112 2142 Size: 30 33 13 30 Fragments arranged by size: 438 327 215 188 170 157 131 105 95 84 37 36 33 32 30 30 13 9 6 6 EarI CTCTTCn'nnn_ Cuts at: 0 118 676 1007 2142 Size: 118 558 331 1135 Fragments arranged by size: 1135 558 331 118 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 214 518 2142 Size: 214 304 1624 Fragments arranged by size: 1624 304 214 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 15 853 1948 2026 2142 Size: 15 838 1095 78 116 Fragments arranged by size: 1095 838 116 78 15 EcoNI CCTnn'n_nnAGG Cuts at: 0 1400 2142 Size: 1400 742 EcoO109I rG'GnC_Cy Cuts at: 0 471 607 886 1048 1049 1164 1390 1391 Size: 471 136 279 162 1 115 226 1 Cuts at: 1391 1494 2142 Size: 103 648 Fragments arranged by size: 648 471 279 226 162 136 115 103 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 93 1890 2142 Size: 93 1797 252 Fragments arranged by size: 1797 252 93 FspI TGC'GCA Cuts at: 0 1606 1690 2142 Size: 1606 84 452 Fragments arranged by size: 1606 452 84 FspAI rTGC'GCAy Cuts at: 0 1690 2142 Size: 1690 452 HaeII r_GCGC'y Cuts at: 0 22 152 326 373 396 645 706 764 Size: 22 130 174 47 23 249 61 58 Cuts at: 764 1008 1069 1339 1816 2005 2090 2142 Size: 244 61 270 477 189 85 52 Fragments arranged by size: 477 270 249 244 189 174 130 85 61 61 58 52 47 23 22 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 2116 2142 Size: 2116 26 HincII GTy'rAC Cuts at: 0 576 2142 Size: 576 1566 KasI G'GCGC_C Cuts at: 0 18 322 702 1065 2142 Size: 18 304 380 363 1077 Fragments arranged by size: 1077 380 363 304 18 MluI A'CGCG_T Cuts at: 0 599 1640 2142 Size: 599 1041 502 Fragments arranged by size: 1041 599 502 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 878 2142 Size: 878 1264 MscI TGG'CCA Cuts at: 0 1184 2026 2142 Size: 1184 842 116 Fragments arranged by size: 1184 842 116 MslI CAynn'nnrTG Cuts at: 0 97 1774 2142 Size: 97 1677 368 Fragments arranged by size: 1677 368 97 MspA1I CmG'CkG Cuts at: 0 39 289 544 571 647 700 717 816 Size: 39 250 255 27 76 53 17 99 Cuts at: 816 875 1296 1372 1466 1920 1951 2022 2142 Size: 59 421 76 94 454 31 71 120 Fragments arranged by size: 454 421 255 250 120 99 94 76 76 71 59 53 39 31 27 17 NaeI GCC'GGC Cuts at: 0 765 1383 1800 2142 Size: 765 618 417 342 Fragments arranged by size: 765 618 417 342 NarI GG'CG_CC Cuts at: 0 19 323 703 1066 2142 Size: 19 304 380 363 1076 Fragments arranged by size: 1076 380 363 304 19 NgoMIV G'CCGG_C Cuts at: 0 763 1381 1798 2142 Size: 763 618 417 344 Fragments arranged by size: 763 618 417 344 NotI GC'GGCC_GC Cuts at: 0 284 712 1946 2142 Size: 284 428 1234 196 Fragments arranged by size: 1234 428 284 196 NspI r_CATG'y Cuts at: 0 1605 1653 1689 2142 Size: 1605 48 36 453 Fragments arranged by size: 1605 453 48 36 PflMI CCAn_nnn'nTGG Cuts at: 0 204 831 2142 Size: 204 627 1311 Fragments arranged by size: 1311 627 204 PmlI CAC'GTG Cuts at: 0 1308 1320 2142 Size: 1308 12 822 Fragments arranged by size: 1308 822 12 PpuMI rG'GwC_Cy Cuts at: 0 607 886 1164 1494 2142 Size: 607 279 278 330 648 Fragments arranged by size: 648 607 330 279 278 PspOMI G'GGCC_C Cuts at: 0 558 1048 1099 1390 2104 2142 Size: 558 490 51 291 714 38 Fragments arranged by size: 714 558 490 291 51 38 PspXI vC'TCGA_Gb Cuts at: 0 1189 2142 Size: 1189 953 PstI C_TGCA'G Cuts at: 0 50 353 2142 Size: 50 303 1789 Fragments arranged by size: 1789 303 50 PvuII CAG'CTG Cuts at: 0 39 1296 1920 2142 Size: 39 1257 624 222 Fragments arranged by size: 1257 624 222 39 SacII CC_GC'GG Cuts at: 0 290 545 572 701 1952 2142 Size: 290 255 27 129 1251 190 Fragments arranged by size: 1251 290 255 190 129 27 SanDI GG'GwC_CC Cuts at: 0 607 886 2142 Size: 607 279 1256 Fragments arranged by size: 1256 607 279 SapI GCTCTTCn'nnn_ Cuts at: 0 676 1007 2142 Size: 676 331 1135 Fragments arranged by size: 1135 676 331 ScaI AGT'ACT Cuts at: 0 406 2142 Size: 406 1736 SfcI C'TryA_G Cuts at: 0 46 349 2142 Size: 46 303 1793 Fragments arranged by size: 1793 303 46 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1357 1366 1375 1804 2142 Size: 1357 9 9 429 338 Fragments arranged by size: 1357 429 338 9 9 SfoI GGC'GCC Cuts at: 0 20 324 704 1067 2142 Size: 20 304 380 363 1075 Fragments arranged by size: 1075 380 363 304 20 SmaI CCC'GGG Cuts at: 0 302 884 1044 2142 Size: 302 582 160 1098 Fragments arranged by size: 1098 582 302 160 SmlI C'TyrA_G Cuts at: 0 157 388 1189 1621 2142 Size: 157 231 801 432 521 Fragments arranged by size: 801 521 432 231 157 SphI G_CATG'C Cuts at: 0 1653 2142 Size: 1653 489 StuI AGG'CCT Cuts at: 0 502 2142 Size: 502 1640 StyI C'CwwG_G Cuts at: 0 68 1386 1994 2142 Size: 68 1318 608 148 Fragments arranged by size: 1318 608 148 68 TatI w'GTAC_w Cuts at: 0 404 2142 Size: 404 1738 Tth111I GACn'n_nGTC Cuts at: 0 1417 2142 Size: 1417 725 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2001 2142 Size: 2001 141 XhoI C'TCGA_G Cuts at: 0 1189 2142 Size: 1189 953 XmaI C'CCGG_G Cuts at: 0 300 882 1042 2142 Size: 300 582 160 1100 Fragments arranged by size: 1100 582 300 160 XmnI GAAnn'nnTTC Cuts at: 0 1570 2142 Size: 1570 572 Enzymes that do cut: AarI AcuI AfeI AflIII AgeI AlwNI ApaI AvaI AvrII BanI BanII BbeI BbsI BbvCI BclI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstAPI BstYI BtgI BtsI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII Hin4I HincII KasI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NotI NspI PflMI PmlI PpuMI PspOMI PspXI PstI PvuII SacII SanDI SapI ScaI SfcI SfiI SfoI SmaI SmlI SphI StuI StyI TatI Tth111I XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI BaeI BamHI BcgI BciVI BfrBI BglII BmtI BsaBI BsaXI BsiWI BspEI BspHI BsrDI BsrGI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI EcoICRI EcoRI EcoRV FseI HindIII HpaI KpnI MfeI NcoI NdeI NheI NruI NsiI PacI PasI PciI PfoI PmeI PpiI PshAI PsiI PsrI PvuI RsrII SacI SalI SbfI SexAI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TaqII TstI XbaI ZraI