################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AviII AvrII BamHI BbeI BbrPI BciVI BclI BfrI BlnI BsaAI BsaBI BsaHI BseAI BsiWI BsmI BspDI BspEI BsrBI BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI DrdI Eco47III EcoO65I EcoRV FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI MamI MroI NarI NdeI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI SalI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AhdI 350 GACCGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 117 CCTGAGG CC^T[ACGT]AGG AspEI 350 GACCGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 494 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BcgI 1773 CGAGGTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 2553 AGATCT A^GATCT BsaWI 2903 TCCGGT [AT]^CCGG[AT] Bsp1407I 1226 TGTACA T^GTACA BspLU11I 2324 ACATGT A^CATGT BsrDI 929 CATTGC GCAATG(2/0) BsrGI 1226 TGTACA T^GTACA BssHII 233 GCGCGC G^CGCGC BstXI 2073 CCAGGCCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 117 CCTGAGG CC^T[ACGT]AGG DraI 1147 TTTAAA TTT^AAA DraIII 2274 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 424 CGGCCG C^GGCCG Eam1105I 350 GACCGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 424 CGGCCG C^GGCCG Eco52I 424 CGGCCG C^GGCCG Eco81I 117 CCTGAGG CC^T[ACGT]AGG EcoRI 875 GAATTC G^AATTC EcoT22I 2391 ATGCAT ATGCA^T MaeII 2699 ACGT A^CGT MfeI 1082 CAATTG C^AATTG MluI 2640 ACGCGT A^CGCGT MunI 1082 CAATTG C^AATTG NheI 1866 GCTAGC G^CTAGC NsiI 2391 ATGCAT ATGCA^T NspI 2324 ACATGT [GA]CATG^[CT] PaeR7I 1771 CTCGAG C^TCGAG PflFI 494 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 514 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 193 GAAGAGC GCTCTTC(1/4) SbfI 124 CCTGCAGG CCTGCA^GG SexAI 2814 ACCAGGT A^CC[AT]GGT SmlI 1771 CTCGAG C^T[CT][GA]AG Sse8387I 124 CCTGCAGG CCTGCA^GG SspBI 1226 TGTACA T^GTACA Tth111I 494 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1771 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcsI 875 GAATTC [GA]^AATT[CT] 2665 AAATTC AflIII 2324 ACATGT A^C[GA][CT]GT 2640 ACGCGT ApoI 875 GAATTC [GA]^AATT[CT] 2665 AAATTC BalI 1709 TGGCCA TGG^CCA 2258 TGGCCA BbvCI 2295 CCTCAGC CCTCAGC(-5/-2) 2026 GCTGAGG BlpI 1803 GCTCAGC GC^T[ACGT]AGC 2822 GCTCAGC Bpu1102I 1803 GCTCAGC GC^T[ACGT]AGC 2822 GCTCAGC BsaI 385 GAGACC GGTCTC(1/5) 2155 GAGACC BsiEI 38 CGACCG CG[GA][CT]^CG 424 CGGCCG BsmBI 418 GAGACG CGTCTC(1/5) 2205 GAGACG BspHI 1764 TCATGA T^CATGA 2790 TCATGA BstAPI 435 GCAAGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2892 GCAGGAGCTGC CelII 1803 GCTCAGC GC^T[ACGT]AGC 2822 GCTCAGC CpoI 348 CGGACCG CG^G[AT]CCG 402 CGGACCG EarI 649 CTCTTC CTCTTC(1/4) 193 GAAGAG Ksp632I 649 CTCTTC CTCTTC(1/4) 193 GAAGAG KspI 406 CCGCGG CCGC^GG 480 CCGCGG MluNI 1709 TGGCCA TGG^CCA 2258 TGGCCA MscI 1709 TGGCCA TGG^CCA 2258 TGGCCA RcaI 1764 TCATGA T^CATGA 2790 TCATGA RsrII 348 CGGACCG CG^G[AT]CCG 402 CGGACCG SacII 406 CCGCGG CCGC^GG 480 CCGCGG XcmI 1190 CCAGTGCCTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2226 CCATGGGCCCCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI