(Circular) (Six-base) MAPSORT of: pFN21AB8543.seq Check: 2897 from: 1 to: 7645 pFN21AB8543 7645 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3962 3962 Size: 7645 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5455 278 Size: 53 83 186 936 3919 2468 Fragments arranged by size: 3919 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4988 1937 Size: 3051 4594 Acc65I G'GTAC_C Cuts at: 4966 4966 Size: 7645 AclI AA'CG_TT Cuts at: 1563 5772 6145 1563 Size: 4209 373 3063 Fragments arranged by size: 4209 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2934 3258 3371 3500 5708 7092 7360 1632 Size: 1302 324 113 129 2208 1384 268 1917 Fragments arranged by size: 2208 1917 1384 1302 324 268 129 113 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7448 AflIII A'CryG_T Cuts at: 1203 4329 4645 6544 7521 1203 Size: 3126 316 1899 977 1327 Fragments arranged by size: 3126 1899 1327 977 316 AgeI A'CCGG_T Cuts at: 6476 6476 Size: 7645 AhdI GACnn_n'nnGTC Cuts at: 2360 6374 2360 Size: 4014 3631 AleI CACnn'nnGTG Cuts at: 1166 3593 3880 1166 Size: 2427 287 4931 Fragments arranged by size: 4931 2427 287 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4411 1184 Size: 3227 4418 AlwNI CAG_nnn'CTG Cuts at: 2227 2513 3501 3739 4051 4903 5388 6960 2227 Size: 286 988 238 312 852 485 1572 2912 Fragments arranged by size: 2912 1572 988 852 485 312 286 238 ApaI G_GGCC'C Cuts at: 2087 2456 3416 3559 3733 3826 4239 2087 Size: 369 960 143 174 93 413 5493 Fragments arranged by size: 5493 960 413 369 174 143 93 ApaLI G'TGCA_C Cuts at: 5702 6858 5702 Size: 1156 6489 ApoI r'AATT_y Cuts at: 1493 2880 4670 4954 5180 5332 7441 1493 Size: 1387 1790 284 226 152 2109 1697 Fragments arranged by size: 2109 1790 1697 1387 284 226 152 AseI AT'TA_AT Cuts at: 160 1032 6199 160 Size: 872 5167 1606 Fragments arranged by size: 5167 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7645 AvaI C'yCGr_G Cuts at: 1870 1943 2078 2210 3552 3776 4970 1870 Size: 73 135 132 1342 224 1194 4545 Fragments arranged by size: 4545 1342 1194 224 135 132 73 BamHI G'GATC_C Cuts at: 4975 5342 4975 Size: 367 7278 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2192 2330 3374 3484 3781 Size: 325 217 627 405 138 1044 110 297 Cuts at: 3781 4244 4966 6421 618 Size: 463 722 1455 1842 Fragments arranged by size: 1842 1455 1044 722 627 463 405 325 297 217 138 110 BanII G_rGCy'C Cuts at: 1391 2037 2087 2144 2267 2352 2456 2484 2799 Size: 646 50 57 123 85 104 28 315 Cuts at: 2799 2877 3257 3416 3471 3559 3733 3777 3826 Size: 78 380 159 55 88 174 44 49 Cuts at: 3826 4093 4239 4323 4964 1391 Size: 267 146 84 641 4072 Fragments arranged by size: 4072 646 641 380 315 267 174 159 146 123 104 88 85 84 78 57 55 50 49 44 28 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2922 2976 3437 928 Size: 971 1023 54 461 5136 Fragments arranged by size: 5136 1023 971 461 54 BbvCI CC'TCA_GC Cuts at: 4032 4301 4032 Size: 269 7376 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3767 3801 5836 5870 1903 Size: 34 1830 34 2035 34 3678 Fragments arranged by size: 3678 2035 1830 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5537 6747 5537 Size: 1210 6435 BclI T'GATC_A Cuts at: 1548 1548 Size: 7645 BfrBI ATG'CAT Cuts at: 4398 4398 Size: 7645 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2834 3136 3206 Size: 107 122 71 631 605 1162 302 70 Cuts at: 3206 3420 6256 136 Size: 214 2836 1525 Fragments arranged by size: 2836 1525 1162 631 605 302 214 122 107 71 70 BglII A'GATC_T Cuts at: 4558 7640 4558 Size: 3082 4563 BlpI GC'TnA_GC Cuts at: 3809 4828 5062 3809 Size: 1019 234 6392 Fragments arranged by size: 6392 1019 234 Bme1580I G_kGCm'C Cuts at: 1790 2087 2195 2323 2456 3379 3416 3489 3559 Size: 297 108 128 133 923 37 73 70 Cuts at: 3559 3733 3826 4239 5706 6862 1790 Size: 174 93 413 1467 1156 2573 Fragments arranged by size: 2573 1467 1156 923 413 297 174 133 128 108 93 73 70 37 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7645 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2270 2393 2777 Size: 416 53 83 399 24 853 123 384 Cuts at: 2777 5376 6334 442 Size: 2599 958 1753 Fragments arranged by size: 2599 1753 958 853 416 399 384 123 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3875 1056 Size: 2819 4826 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2411 2816 2411 Size: 405 7240 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2051 2195 2441 2570 2628 3165 3294 3313 Size: 812 144 246 129 58 537 129 19 Cuts at: 3313 3338 3347 3478 4073 4178 4199 4479 4823 Size: 25 9 131 595 105 21 280 344 Cuts at: 4823 6305 1239 Size: 1482 2579 Fragments arranged by size: 2579 1482 812 595 537 344 280 246 144 131 129 129 105 58 25 21 19 9 Bpu10I CC'TnA_GC Cuts at: 3251 4032 4188 4216 4301 3251 Size: 781 156 28 85 6595 Fragments arranged by size: 6595 781 156 85 28 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5770 6635 6933 5770 Size: 865 298 6482 Fragments arranged by size: 6482 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2384 4154 6308 882 Size: 617 885 1770 2154 2219 Fragments arranged by size: 2219 2154 1770 885 617 BsaAI yAC'GTr Cuts at: 493 1204 5436 493 Size: 711 4232 2702 Fragments arranged by size: 4232 2702 711 BsaBI GATnn'nnATC Cuts at: 7639 7639 Size: 7645 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5452 5834 6469 275 Size: 53 83 186 936 3919 382 635 1451 Fragments arranged by size: 3919 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4908 6078 6476 6750 6897 7308 4908 Size: 1170 398 274 147 411 5245 Fragments arranged by size: 5245 1170 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4545 4575 4619 4649 1178 Size: 30 165 30 3142 30 44 30 4174 Fragments arranged by size: 4174 3142 165 44 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3297 3656 3659 4001 4364 4476 1186 Size: 2111 359 3 342 363 112 4355 Fragments arranged by size: 4355 2111 363 359 342 112 3 BseYI C'CCAG_C Cuts at: 2104 2338 2449 2748 2788 3625 4638 4725 6848 Size: 234 111 299 40 837 1013 87 2123 Cuts at: 6848 7344 2104 Size: 496 2405 Fragments arranged by size: 2405 2123 1013 837 496 299 234 111 87 40 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2750 3525 3773 3906 3928 4306 4496 Size: 573 1014 775 248 133 22 378 190 Cuts at: 4496 1163 Size: 4312 Fragments arranged by size: 4312 1014 775 573 378 248 190 133 22 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2046 2432 5095 5856 6884 1540 Size: 275 186 45 386 2663 761 1028 2301 Fragments arranged by size: 2663 2301 1028 761 386 275 186 45 BsiHKAI G_wGCw'C Cuts at: 2484 2877 3777 4185 4323 5706 5791 6862 2484 Size: 393 900 408 138 1383 85 1071 3267 Fragments arranged by size: 3267 1383 1071 900 408 393 138 85 BsmI GAATG_Cn' Cuts at: 5156 5249 5156 Size: 93 7552 BsmBI CGTCTCn'nnnn_ Cuts at: 2417 4204 2417 Size: 1787 5858 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2087 2144 2195 2267 2323 2352 Size: 399 247 50 57 51 72 56 29 Cuts at: 2352 2456 2484 2799 2877 3257 3379 3416 3471 Size: 104 28 315 78 380 122 37 55 Cuts at: 3471 3489 3559 3733 3777 3826 4093 4185 4239 Size: 18 70 174 44 49 267 92 54 Cuts at: 4239 4323 4964 5706 5791 6862 1391 Size: 84 641 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 641 399 380 315 267 247 174 122 104 92 85 84 78 72 70 57 56 55 54 51 50 49 44 37 29 28 18 BspEI T'CCGG_A Cuts at: 7308 7308 Size: 7645 BspHI T'CATG_A Cuts at: 3769 4795 5534 7264 3769 Size: 1026 739 1730 4150 Fragments arranged by size: 4150 1730 1026 739 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2722 3747 3962 5000 844 Size: 1878 1025 215 1038 3489 Fragments arranged by size: 3489 1878 1038 1025 215 BsrBI CCG'CTC Cuts at: 1394 1823 5532 1394 Size: 429 3709 3507 Fragments arranged by size: 3709 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2933 6140 6314 65 Size: 1183 1685 3207 174 1396 Fragments arranged by size: 3207 1685 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2092 2152 2431 3030 3567 4013 4247 Size: 110 282 60 279 599 537 446 234 Cuts at: 4247 6289 6476 1700 Size: 2042 187 2869 Fragments arranged by size: 2869 2042 599 537 446 282 279 234 187 110 60 BsrGI T'GTAC_A Cuts at: 96 3231 96 Size: 3135 4510 BssHII G'CGCG_C Cuts at: 1925 2238 1925 Size: 313 7332 BssSI C'ACGA_G Cuts at: 5705 6717 5705 Size: 1012 6633 BstAPI GCAn_nnn'nTGC Cuts at: 794 2446 4903 794 Size: 1652 2457 3536 Fragments arranged by size: 3536 2457 1652 BstBI TT'CG_AA Cuts at: 6449 6449 Size: 7645 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7645 BstXI CCAn_nnnn'nTGG Cuts at: 2011 4085 2011 Size: 2074 5571 BstYI r'GATC_y Cuts at: 1972 3102 3904 4098 4152 4558 4975 5342 5727 Size: 1130 802 194 54 406 417 367 385 Cuts at: 5727 5744 7196 7282 7294 7640 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1130 802 417 406 385 367 346 194 86 54 17 12 Bsu36I CC'TnA_GG Cuts at: 2123 2123 Size: 7645 BtgI C'CryG_G Cuts at: 513 1065 2004 2087 2411 2485 2544 2641 3263 Size: 552 939 83 324 74 59 97 622 Cuts at: 3263 4231 4845 513 Size: 968 614 3313 Fragments arranged by size: 3313 968 939 622 614 552 324 97 83 74 59 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2890 487 Size: 683 56 1664 5242 Fragments arranged by size: 5242 1664 683 56 BtsI GCAGTG_nn' Cuts at: 2070 2321 3403 3828 5161 5955 5975 2070 Size: 251 1082 425 1333 794 20 3740 Fragments arranged by size: 3740 1333 1082 794 425 251 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7645 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3424 3459 529 Size: 35 2860 35 4715 Fragments arranged by size: 4715 2860 35 35 DraI TTT'AAA Cuts at: 3154 4949 3154 Size: 1795 5850 DraIII CAC_nnn'GTG Cuts at: 4284 4284 Size: 7645 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6652 809 Size: 507 5336 1802 Fragments arranged by size: 5336 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2090 2429 3207 Size: 54 1425 50 275 18 260 339 778 Cuts at: 3207 3714 4263 5092 5981 7610 8 Size: 507 549 829 889 1629 43 Fragments arranged by size: 1629 1425 889 829 778 549 507 339 275 260 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2429 5092 1537 Size: 275 617 2663 4090 Fragments arranged by size: 4090 2663 617 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2193 2660 5575 1353 Size: 840 467 2915 3423 Fragments arranged by size: 3423 2915 840 467 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2114 6232 6606 6752 387 Size: 1064 138 525 4118 374 146 1280 Fragments arranged by size: 4118 1280 1064 525 374 146 138 EcoICRI GAG'CTC Cuts at: 2482 2875 3775 4321 2482 Size: 393 900 546 5806 Fragments arranged by size: 5806 900 546 393 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2195 2441 2570 2628 2934 3165 Size: 393 419 144 246 129 58 306 231 Cuts at: 3165 3258 3294 3313 3338 3347 3371 3478 3500 Size: 93 36 19 25 9 24 107 22 Cuts at: 3500 4073 4178 4199 4479 4823 5708 6305 7092 Size: 573 105 21 280 344 885 597 787 Cuts at: 7092 7360 1239 Size: 268 1524 Fragments arranged by size: 1524 885 787 597 573 419 393 344 306 280 268 246 231 144 129 107 105 93 58 36 25 24 22 21 19 9 EcoNI CCTnn'n_nnAGG Cuts at: 2657 3674 3848 3946 4031 2657 Size: 1017 174 98 85 6271 Fragments arranged by size: 6271 1017 174 98 85 EcoO109I rG'GnC_Cy Cuts at: 2026 2083 2207 2271 2342 2734 2752 2771 3412 Size: 57 124 64 71 392 18 19 641 Cuts at: 3412 3413 3679 3736 3823 4236 2026 Size: 1 266 57 87 413 5435 Fragments arranged by size: 5435 641 413 392 266 124 87 71 64 57 57 19 18 1 EcoRI G'AATT_C Cuts at: 2880 4954 5332 2880 Size: 2074 378 5193 Fragments arranged by size: 5193 2074 378 FspI TGC'GCA Cuts at: 6151 6151 Size: 7645 HaeII r_GCGC'y Cuts at: 1678 2012 2159 2312 2819 6792 1678 Size: 334 147 153 507 3973 2531 Fragments arranged by size: 3973 2531 507 334 153 147 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2346 2378 4545 4577 4619 Size: 32 135 32 774 32 2167 32 42 Cuts at: 4619 4651 4733 4765 6286 6318 6360 6392 1373 Size: 32 82 32 1521 32 42 32 2626 Fragments arranged by size: 2626 2167 1521 774 135 82 42 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4989 5235 669 Size: 988 281 3051 246 3079 Fragments arranged by size: 3079 3051 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7645 HpaI GTT'AAC Cuts at: 5235 5235 Size: 7645 KpnI G_GTAC'C Cuts at: 4970 4970 Size: 7645 MfeI C'AATT_G Cuts at: 3087 5244 3087 Size: 2157 5488 MluI A'CGCG_T Cuts at: 4645 7521 4645 Size: 2876 4769 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2066 4066 6759 6943 1305 Size: 761 2000 2693 184 2007 Fragments arranged by size: 2693 2007 2000 761 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3716 4265 10 Size: 54 1425 343 1884 549 3390 Fragments arranged by size: 3390 1884 1425 549 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3593 3710 3880 4328 4334 5603 5962 Size: 648 2427 117 170 448 6 1269 359 Cuts at: 5962 6121 518 Size: 159 2042 Fragments arranged by size: 2427 2042 1269 648 448 359 170 159 117 6 MspA1I CmG'CkG Cuts at: 1606 2371 2413 2487 2788 3593 3693 3805 4729 Size: 765 42 74 301 805 100 112 924 Cuts at: 4729 4775 5061 5738 6886 7131 1606 Size: 46 286 677 1148 245 2120 Fragments arranged by size: 2120 1148 924 805 765 677 301 286 245 112 100 74 46 42 NaeI GCC'GGC Cuts at: 2094 2154 2433 3569 4015 2094 Size: 60 279 1136 446 5724 Fragments arranged by size: 5724 1136 446 279 60 NcoI C'CATG_G Cuts at: 513 1065 2004 3263 4231 4845 513 Size: 552 939 1259 968 614 3313 Fragments arranged by size: 3313 1259 968 939 614 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7645 NgoMIV G'CCGG_C Cuts at: 2092 2152 2431 3567 4013 2092 Size: 60 279 1136 446 5724 Fragments arranged by size: 5724 1136 446 279 60 NheI G'CTAG_C Cuts at: 1052 3871 1052 Size: 2819 4826 NotI GC'GGCC_GC Cuts at: 5092 5092 Size: 7645 NsiI A_TGCA'T Cuts at: 4400 4400 Size: 7645 NspI r_CATG'y Cuts at: 1132 1229 4333 5003 6548 1132 Size: 97 3104 670 1545 2229 Fragments arranged by size: 3104 2229 1545 670 97 PasI CC'CwG_GG Cuts at: 1348 2456 3048 3844 1348 Size: 1108 592 796 5149 Fragments arranged by size: 5149 1108 796 592 PciI A'CATG_T Cuts at: 4329 6544 4329 Size: 2215 5430 PflMI CCAn_nnn'nTGG Cuts at: 1108 2513 3089 3291 3425 1108 Size: 1405 576 202 134 5328 Fragments arranged by size: 5328 1405 576 202 134 PfoI T'CCnGG_A Cuts at: 2028 2404 3057 3105 4155 4563 2028 Size: 376 653 48 1050 408 5110 Fragments arranged by size: 5110 1050 653 408 376 48 PmeI GTTT'AAAC Cuts at: 4949 4949 Size: 7645 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5714 5746 7227 7259 5714 Size: 32 1481 32 6100 Fragments arranged by size: 6100 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2026 2207 2734 2752 2771 3679 3736 2026 Size: 181 527 18 19 908 57 5935 Fragments arranged by size: 5935 908 527 181 57 19 18 PshAI GACnn'nnGTC Cuts at: 1613 2523 1613 Size: 910 6735 PsiI TTA'TAA Cuts at: 5215 5215 Size: 7645 PspOMI G'GGCC_C Cuts at: 2083 2452 3412 3555 3729 3822 4235 2083 Size: 369 960 143 174 93 413 5493 Fragments arranged by size: 5493 960 413 369 174 143 93 PspXI vC'TCGA_Gb Cuts at: 3776 3776 Size: 7645 PstI C_TGCA'G Cuts at: 830 2134 3540 3577 4065 4137 4390 4863 4997 Size: 1304 1406 37 488 72 253 473 134 Cuts at: 4997 830 Size: 3478 Fragments arranged by size: 3478 1406 1304 488 473 253 134 72 37 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7645 PvuII CAG'CTG Cuts at: 3593 3693 4729 4775 3593 Size: 100 1036 46 6463 Fragments arranged by size: 6463 1036 100 46 RsrII CG'GwC_CG Cuts at: 2354 2408 2354 Size: 54 7591 SacI G_AGCT'C Cuts at: 2484 2877 3777 4323 2484 Size: 393 900 546 5806 Fragments arranged by size: 5806 900 546 393 SacII CC_GC'GG Cuts at: 2414 2488 2414 Size: 74 7571 SalI G'TCGA_C Cuts at: 1936 4987 1936 Size: 3051 4594 SanDI GG'GwC_CC Cuts at: 2207 2771 2207 Size: 564 7081 SapI GCTCTTCn'nnn_ Cuts at: 2193 2193 Size: 7645 SbfI CC_TGCA'GG Cuts at: 2134 4997 2134 Size: 2863 4782 ScaI AGT'ACT Cuts at: 5893 7605 5893 Size: 1712 5933 SexAI A'CCwGG_T Cuts at: 4819 4819 Size: 7645 SfcI C'TryA_G Cuts at: 826 1045 2130 2995 3454 3536 3573 4061 4133 Size: 219 1085 865 459 82 37 488 72 Cuts at: 4133 4386 4859 4993 6128 6809 7000 826 Size: 253 473 134 1135 681 191 1471 Fragments arranged by size: 1471 1135 1085 865 681 488 473 459 253 219 191 134 82 72 37 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7645 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7645 SmaI CCC'GGG Cuts at: 1872 2080 2212 3554 4972 1872 Size: 208 132 1342 1418 4545 Fragments arranged by size: 4545 1418 1342 208 132 SmlI C'TyrA_G Cuts at: 820 1017 1943 3776 5749 6650 6912 820 Size: 197 926 1833 1973 901 262 1553 Fragments arranged by size: 1973 1833 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5436 493 Size: 4943 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7645 SphI G_CATG'C Cuts at: 1132 5003 1132 Size: 3871 3774 SspI AAT'ATT Cuts at: 5 52 5569 5 Size: 47 5517 2081 Fragments arranged by size: 5517 2081 47 StuI AGG'CCT Cuts at: 1517 2128 3315 3323 4221 1517 Size: 611 1187 8 898 4941 Fragments arranged by size: 4941 1187 898 611 8 StyI C'CwwG_G Cuts at: 513 1065 2004 2647 3263 3324 3463 4231 4266 Size: 552 939 643 616 61 139 768 35 Cuts at: 4266 4845 5084 513 Size: 579 239 3074 Fragments arranged by size: 3074 939 768 643 616 579 552 239 139 61 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2060 2266 2659 2665 3233 3617 4115 Size: 1255 480 206 393 6 568 384 498 Cuts at: 4115 5672 5689 5842 6027 6472 325 Size: 1557 17 153 185 445 1498 Fragments arranged by size: 1557 1498 1255 568 498 480 445 393 384 206 185 153 17 6 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3231 5891 7603 Size: 274 80 33 51 1443 1254 2660 1712 Cuts at: 7603 96 Size: 138 Fragments arranged by size: 2660 1712 1443 1254 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2400 2432 7605 7637 2400 Size: 32 5173 32 2408 Fragments arranged by size: 5173 2408 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2502 1316 Size: 1186 6459 XbaI T'CTAG_A Cuts at: 4981 4981 Size: 7645 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3202 4238 3202 Size: 1036 6609 XhoI C'TCGA_G Cuts at: 1943 3776 1943 Size: 1833 5812 XmaI C'CCGG_G Cuts at: 1870 2078 2210 3552 4970 1870 Size: 208 132 1342 1418 4545 Fragments arranged by size: 4545 1418 1342 208 132 XmnI GAAnn'nnTTC Cuts at: 5774 5774 Size: 7645 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5453 276 Size: 53 83 186 936 3919 2468 Fragments arranged by size: 3919 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BsiWI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PsrI SfiI SfoI SrfI SwaI