(Linear) (Six-base) MAPSORT of: KIBB8543.seq Check: 1252 from: 1 to: 2940 KIBB8543.orf With 182 enzymes: SgfI * July 15, 2010 18:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1957 2940 Size: 1957 983 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 929 1253 1366 1495 2940 Size: 929 324 113 129 1445 Fragments arranged by size: 1445 929 324 129 113 AflIII A'CryG_T Cuts at: 0 2324 2640 2940 Size: 2324 316 300 Fragments arranged by size: 2324 316 300 AhdI GACnn_n'nnGTC Cuts at: 0 355 2940 Size: 355 2585 AleI CACnn'nnGTG Cuts at: 0 1588 1875 2940 Size: 1588 287 1065 Fragments arranged by size: 1588 1065 287 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2406 2940 Size: 2406 534 AlwNI CAG_nnn'CTG Cuts at: 0 222 508 1496 1734 2046 2898 2940 Size: 222 286 988 238 312 852 42 Fragments arranged by size: 988 852 312 286 238 222 42 ApaI G_GGCC'C Cuts at: 0 82 451 1411 1554 1728 1821 2234 2940 Size: 82 369 960 143 174 93 413 706 Fragments arranged by size: 960 706 413 369 174 143 93 82 ApoI r'AATT_y Cuts at: 0 875 2665 2940 Size: 875 1790 275 Fragments arranged by size: 1790 875 275 AvaI C'yCGr_G Cuts at: 0 73 205 1547 1771 2940 Size: 73 132 1342 224 1169 Fragments arranged by size: 1342 1169 224 132 73 BanI G'GyrC_C Cuts at: 0 187 325 1369 1479 1776 2239 2940 Size: 187 138 1044 110 297 463 701 Fragments arranged by size: 1044 701 463 297 187 138 110 BanII G_rGCy'C Cuts at: 0 32 82 139 262 347 451 479 794 Size: 32 50 57 123 85 104 28 315 Cuts at: 794 872 1252 1411 1466 1554 1728 1772 1821 Size: 78 380 159 55 88 174 44 49 Cuts at: 1821 2088 2234 2318 2940 Size: 267 146 84 622 Fragments arranged by size: 622 380 315 267 174 159 146 123 104 88 85 84 78 57 55 50 49 44 32 28 BbsI GAAGACnn'nnnn_ Cuts at: 0 917 971 1432 2940 Size: 917 54 461 1508 Fragments arranged by size: 1508 917 461 54 BbvCI CC'TCA_GC Cuts at: 0 2027 2296 2940 Size: 2027 269 644 Fragments arranged by size: 2027 644 269 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1762 1796 2940 Size: 1762 34 1144 Fragments arranged by size: 1762 1144 34 BfrBI ATG'CAT Cuts at: 0 2393 2940 Size: 2393 547 BglI GCCn_nnn'nGGC Cuts at: 0 829 1131 1201 1415 2940 Size: 829 302 70 214 1525 Fragments arranged by size: 1525 829 302 214 70 BglII A'GATC_T Cuts at: 0 2553 2940 Size: 2553 387 BlpI GC'TnA_GC Cuts at: 0 1804 2823 2940 Size: 1804 1019 117 Fragments arranged by size: 1804 1019 117 Bme1580I G_kGCm'C Cuts at: 0 82 190 318 451 1374 1411 1484 1554 Size: 82 108 128 133 923 37 73 70 Cuts at: 1554 1728 1821 2234 2940 Size: 174 93 413 706 Fragments arranged by size: 923 706 413 174 133 128 108 93 82 73 70 37 BmrI ACTGGGnnnn_n' Cuts at: 0 265 388 772 2940 Size: 265 123 384 2168 Fragments arranged by size: 2168 384 265 123 BmtI G_CTAG'C Cuts at: 0 1870 2940 Size: 1870 1070 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 406 811 2940 Size: 406 405 2129 Fragments arranged by size: 2129 406 405 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 46 190 436 565 623 1160 1289 1308 Size: 46 144 246 129 58 537 129 19 Cuts at: 1308 1333 1342 1473 2068 2173 2194 2474 2818 Size: 25 9 131 595 105 21 280 344 Cuts at: 2818 2940 Size: 122 Fragments arranged by size: 595 537 344 280 246 144 131 129 129 122 105 58 46 25 21 19 9 Bpu10I CC'TnA_GC Cuts at: 0 1246 2027 2183 2211 2296 2940 Size: 1246 781 156 28 85 644 Fragments arranged by size: 1246 781 644 156 85 28 BsaI GGTCTCn'nnnn_ Cuts at: 0 379 2149 2940 Size: 379 1770 791 Fragments arranged by size: 1770 791 379 BsaWI w'CCGG_w Cuts at: 0 2903 2940 Size: 2903 37 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2540 2570 2614 2644 2940 Size: 2540 30 44 30 296 Fragments arranged by size: 2540 296 44 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1292 1651 1654 1996 2359 2471 2940 Size: 1292 359 3 342 363 112 469 Fragments arranged by size: 1292 469 363 359 342 112 3 BseYI C'CCAG_C Cuts at: 0 99 333 444 743 783 1620 2633 2720 Size: 99 234 111 299 40 837 1013 87 Cuts at: 2720 2940 Size: 220 Fragments arranged by size: 1013 837 299 234 220 111 99 87 40 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 745 1520 1768 1901 1923 2301 2491 2940 Size: 745 775 248 133 22 378 190 449 Fragments arranged by size: 775 745 449 378 248 190 133 22 BsiEI CG_ry'CG Cuts at: 0 41 427 2940 Size: 41 386 2513 Fragments arranged by size: 2513 386 41 BsiHKAI G_wGCw'C Cuts at: 0 479 872 1772 2180 2318 2940 Size: 479 393 900 408 138 622 Fragments arranged by size: 900 622 479 408 393 138 BsmBI CGTCTCn'nnnn_ Cuts at: 0 412 2199 2940 Size: 412 1787 741 Fragments arranged by size: 1787 741 412 Bsp1286I G_dGCh'C Cuts at: 0 32 82 139 190 262 318 347 451 Size: 32 50 57 51 72 56 29 104 Cuts at: 451 479 794 872 1252 1374 1411 1466 1484 Size: 28 315 78 380 122 37 55 18 Cuts at: 1484 1554 1728 1772 1821 2088 2180 2234 2318 Size: 70 174 44 49 267 92 54 84 Cuts at: 2318 2940 Size: 622 Fragments arranged by size: 622 380 315 267 174 122 104 92 84 78 72 70 57 56 55 54 51 50 49 44 37 32 29 28 18 BspHI T'CATG_A Cuts at: 0 1764 2790 2940 Size: 1764 1026 150 Fragments arranged by size: 1764 1026 150 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 717 1742 1957 2940 Size: 717 1025 215 983 Fragments arranged by size: 1025 983 717 215 BsrDI GCAATG_nn' Cuts at: 0 928 2940 Size: 928 2012 BsrFI r'CCGG_y Cuts at: 0 87 147 426 1025 1562 2008 2242 2940 Size: 87 60 279 599 537 446 234 698 Fragments arranged by size: 698 599 537 446 279 234 87 60 BsrGI T'GTAC_A Cuts at: 0 1226 2940 Size: 1226 1714 BssHII G'CGCG_C Cuts at: 0 233 2940 Size: 233 2707 BstAPI GCAn_nnn'nTGC Cuts at: 0 441 2898 2940 Size: 441 2457 42 Fragments arranged by size: 2457 441 42 BstXI CCAn_nnnn'nTGG Cuts at: 0 2080 2940 Size: 2080 860 BstYI r'GATC_y Cuts at: 0 1097 1899 2093 2147 2553 2940 Size: 1097 802 194 54 406 387 Fragments arranged by size: 1097 802 406 387 194 54 Bsu36I CC'TnA_GG Cuts at: 0 118 2940 Size: 118 2822 BtgI C'CryG_G Cuts at: 0 82 406 480 539 636 1258 2226 2840 Size: 82 324 74 59 97 622 968 614 Cuts at: 2840 2940 Size: 100 Fragments arranged by size: 968 622 614 324 100 97 82 74 59 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 885 2940 Size: 885 2055 BtsI GCAGTG_nn' Cuts at: 0 65 316 1398 1823 2940 Size: 65 251 1082 425 1117 Fragments arranged by size: 1117 1082 425 251 65 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1419 1454 2940 Size: 1419 35 1486 Fragments arranged by size: 1486 1419 35 DraI TTT'AAA Cuts at: 0 1149 2940 Size: 1149 1791 DraIII CAC_nnn'GTG Cuts at: 0 2279 2940 Size: 2279 661 EaeI y'GGCC_r Cuts at: 0 85 424 1202 1709 2258 2940 Size: 85 339 778 507 549 682 Fragments arranged by size: 778 682 549 507 339 85 EagI C'GGCC_G Cuts at: 0 424 2940 Size: 424 2516 EarI CTCTTCn'nnn_ Cuts at: 0 188 655 2940 Size: 188 467 2285 Fragments arranged by size: 2285 467 188 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 109 2940 Size: 109 2831 EcoICRI GAG'CTC Cuts at: 0 477 870 1770 2316 2940 Size: 477 393 900 546 624 Fragments arranged by size: 900 624 546 477 393 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 46 190 436 565 623 929 1160 1253 Size: 46 144 246 129 58 306 231 93 Cuts at: 1253 1289 1308 1333 1342 1366 1473 1495 2068 Size: 36 19 25 9 24 107 22 573 Cuts at: 2068 2173 2194 2474 2818 2940 Size: 105 21 280 344 122 Fragments arranged by size: 573 344 306 280 246 231 144 129 122 107 105 93 58 46 36 25 24 22 21 19 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 652 1669 1843 1941 2026 2940 Size: 652 1017 174 98 85 914 Fragments arranged by size: 1017 914 652 174 98 85 EcoO109I rG'GnC_Cy Cuts at: 0 21 78 202 266 337 729 747 766 Size: 21 57 124 64 71 392 18 19 Cuts at: 766 1407 1408 1674 1731 1818 2231 2940 Size: 641 1 266 57 87 413 709 Fragments arranged by size: 709 641 413 392 266 124 87 71 64 57 57 21 19 18 1 EcoRI G'AATT_C Cuts at: 0 875 2940 Size: 875 2065 HaeII r_GCGC'y Cuts at: 0 7 154 307 814 2940 Size: 7 147 153 507 2126 Fragments arranged by size: 2126 507 153 147 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 341 373 2540 2572 2614 2646 2728 2760 Size: 341 32 2167 32 42 32 82 32 Cuts at: 2760 2940 Size: 180 Fragments arranged by size: 2167 341 180 82 42 32 32 32 32 MfeI C'AATT_G Cuts at: 0 1082 2940 Size: 1082 1858 MluI A'CGCG_T Cuts at: 0 2640 2940 Size: 2640 300 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 61 2061 2940 Size: 61 2000 879 Fragments arranged by size: 2000 879 61 MscI TGG'CCA Cuts at: 0 1711 2260 2940 Size: 1711 549 680 Fragments arranged by size: 1711 680 549 MslI CAynn'nnrTG Cuts at: 0 1588 1705 1875 2323 2329 2940 Size: 1588 117 170 448 6 611 Fragments arranged by size: 1588 611 448 170 117 6 MspA1I CmG'CkG Cuts at: 0 366 408 482 783 1588 1688 1800 2724 Size: 366 42 74 301 805 100 112 924 Cuts at: 2724 2770 2940 Size: 46 170 Fragments arranged by size: 924 805 366 301 170 112 100 74 46 42 NaeI GCC'GGC Cuts at: 0 89 149 428 1564 2010 2940 Size: 89 60 279 1136 446 930 Fragments arranged by size: 1136 930 446 279 89 60 NcoI C'CATG_G Cuts at: 0 1258 2226 2840 2940 Size: 1258 968 614 100 Fragments arranged by size: 1258 968 614 100 NgoMIV G'CCGG_C Cuts at: 0 87 147 426 1562 2008 2940 Size: 87 60 279 1136 446 932 Fragments arranged by size: 1136 932 446 279 87 60 NheI G'CTAG_C Cuts at: 0 1866 2940 Size: 1866 1074 NsiI A_TGCA'T Cuts at: 0 2395 2940 Size: 2395 545 NspI r_CATG'y Cuts at: 0 2328 2940 Size: 2328 612 PasI CC'CwG_GG Cuts at: 0 451 1043 1839 2940 Size: 451 592 796 1101 Fragments arranged by size: 1101 796 592 451 PciI A'CATG_T Cuts at: 0 2324 2940 Size: 2324 616 PflMI CCAn_nnn'nTGG Cuts at: 0 508 1084 1286 1420 2940 Size: 508 576 202 134 1520 Fragments arranged by size: 1520 576 508 202 134 PfoI T'CCnGG_A Cuts at: 0 23 399 1052 1100 2150 2558 2940 Size: 23 376 653 48 1050 408 382 Fragments arranged by size: 1050 653 408 382 376 48 23 PpuMI rG'GwC_Cy Cuts at: 0 21 202 729 747 766 1674 1731 2940 Size: 21 181 527 18 19 908 57 1209 Fragments arranged by size: 1209 908 527 181 57 21 19 18 PshAI GACnn'nnGTC Cuts at: 0 518 2940 Size: 518 2422 PspOMI G'GGCC_C Cuts at: 0 78 447 1407 1550 1724 1817 2230 2940 Size: 78 369 960 143 174 93 413 710 Fragments arranged by size: 960 710 413 369 174 143 93 78 PspXI vC'TCGA_Gb Cuts at: 0 1771 2940 Size: 1771 1169 PstI C_TGCA'G Cuts at: 0 129 1535 1572 2060 2132 2385 2858 2940 Size: 129 1406 37 488 72 253 473 82 Fragments arranged by size: 1406 488 473 253 129 82 72 37 PvuII CAG'CTG Cuts at: 0 1588 1688 2724 2770 2940 Size: 1588 100 1036 46 170 Fragments arranged by size: 1588 1036 170 100 46 RsrII CG'GwC_CG Cuts at: 0 349 403 2940 Size: 349 54 2537 Fragments arranged by size: 2537 349 54 SacI G_AGCT'C Cuts at: 0 479 872 1772 2318 2940 Size: 479 393 900 546 622 Fragments arranged by size: 900 622 546 479 393 SacII CC_GC'GG Cuts at: 0 409 483 2940 Size: 409 74 2457 Fragments arranged by size: 2457 409 74 SanDI GG'GwC_CC Cuts at: 0 202 766 2940 Size: 202 564 2174 Fragments arranged by size: 2174 564 202 SapI GCTCTTCn'nnn_ Cuts at: 0 188 2940 Size: 188 2752 SbfI CC_TGCA'GG Cuts at: 0 129 2940 Size: 129 2811 SexAI A'CCwGG_T Cuts at: 0 2814 2940 Size: 2814 126 SfcI C'TryA_G Cuts at: 0 125 990 1449 1531 1568 2056 2128 2381 Size: 125 865 459 82 37 488 72 253 Cuts at: 2381 2854 2940 Size: 473 86 Fragments arranged by size: 865 488 473 459 253 125 86 82 72 37 SmaI CCC'GGG Cuts at: 0 75 207 1549 2940 Size: 75 132 1342 1391 Fragments arranged by size: 1391 1342 132 75 SmlI C'TyrA_G Cuts at: 0 1771 2940 Size: 1771 1169 StuI AGG'CCT Cuts at: 0 123 1310 1318 2216 2940 Size: 123 1187 8 898 724 Fragments arranged by size: 1187 898 724 123 8 StyI C'CwwG_G Cuts at: 0 642 1258 1319 1458 2226 2261 2840 2940 Size: 642 616 61 139 768 35 579 100 Fragments arranged by size: 768 642 616 579 139 100 61 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 55 261 654 660 1228 1612 2110 2940 Size: 55 206 393 6 568 384 498 830 Fragments arranged by size: 830 568 498 393 384 206 55 6 TatI w'GTAC_w Cuts at: 0 1226 2940 Size: 1226 1714 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 395 427 2940 Size: 395 32 2513 Fragments arranged by size: 2513 395 32 Tth111I GACn'n_nGTC Cuts at: 0 497 2940 Size: 497 2443 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1197 2233 2940 Size: 1197 1036 707 Fragments arranged by size: 1197 1036 707 XhoI C'TCGA_G Cuts at: 0 1771 2940 Size: 1771 1169 XmaI C'CCGG_G Cuts at: 0 73 205 1547 2940 Size: 73 132 1342 1393 Fragments arranged by size: 1393 1342 132 73 Enzymes that do cut: AarI AcuI AflIII AhdI AleI AlfI AlwNI ApaI ApoI AvaI BanI BanII BbsI BbvCI BcgI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BsaI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssHII BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NheI NsiI NspI PasI PciI PflMI PfoI PpuMI PshAI PspOMI PspXI PstI PvuII RsrII SacI SacII SanDI SapI SbfI SexAI SfcI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI BclI BmgBI BpuEI BsaAI BsaBI BsaHI BsiWI BsmI BspEI BsrBI BssSI BstBI BstEII BstZ17I ClaI DrdI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI NarI NdeI NotI NruI PacI PmeI PmlI PpiI PsiI PsrI PvuI SalI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI XmnI ZraI