(Circular) (Six-base) MAPSORT of: pFN21AE0380.seq Check: 2146 from: 1 to: 6448 pFN21AE0380 6448 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4258 278 Size: 53 83 186 936 2722 2468 Fragments arranged by size: 2722 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3791 1937 Size: 1854 4594 Acc65I G'GTAC_C Cuts at: 3769 3769 Size: 6448 AclI AA'CG_TT Cuts at: 1563 4575 4948 1563 Size: 3012 373 3063 Fragments arranged by size: 3063 3012 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2573 3177 4511 5895 6163 1632 Size: 941 604 1334 1384 268 1917 Fragments arranged by size: 1917 1384 1334 941 604 268 AfeI AGC'GCT Cuts at: 2363 2561 2363 Size: 198 6250 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6251 AflIII A'CryG_T Cuts at: 1203 3474 5347 6324 1203 Size: 2271 1873 977 1327 Fragments arranged by size: 2271 1873 1327 977 AgeI A'CCGG_T Cuts at: 5279 5279 Size: 6448 AhdI GACnn_n'nnGTC Cuts at: 3422 5177 3422 Size: 1755 4693 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6448 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2357 2779 1184 Size: 1173 422 4853 Fragments arranged by size: 4853 1173 422 AlwNI CAG_nnn'CTG Cuts at: 2876 4191 5763 2876 Size: 1315 1572 3561 Fragments arranged by size: 3561 1572 1315 ApaLI G'TGCA_C Cuts at: 4505 5661 4505 Size: 1156 5292 ApoI r'AATT_y Cuts at: 1493 3026 3173 3757 3983 4135 6244 1493 Size: 1533 147 584 226 152 2109 1697 Fragments arranged by size: 2109 1697 1533 584 226 152 147 AseI AT'TA_AT Cuts at: 160 1032 5002 160 Size: 872 3970 1606 Fragments arranged by size: 3970 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6448 AvaI C'yCGr_G Cuts at: 1870 1943 2177 2357 3773 1870 Size: 73 234 180 1416 4545 Fragments arranged by size: 4545 1416 234 180 73 BamHI G'GATC_C Cuts at: 2736 3778 4145 2736 Size: 1042 367 5039 Fragments arranged by size: 5039 1042 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2212 3769 5224 618 Size: 325 217 627 425 1557 1455 1842 Fragments arranged by size: 1842 1557 1455 627 425 325 217 BanII G_rGCy'C Cuts at: 1391 2108 2638 2826 3186 3767 1391 Size: 717 530 188 360 581 4072 Fragments arranged by size: 4072 717 581 530 360 188 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5477 BbvCI CC'TCA_GC Cuts at: 2324 2324 Size: 6448 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3557 3591 4639 4673 1903 Size: 34 1620 34 1048 34 3678 Fragments arranged by size: 3678 1620 1048 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2883 3312 3538 4340 5550 2883 Size: 429 226 802 1210 3781 Fragments arranged by size: 3781 1210 802 429 226 BclI T'GATC_A Cuts at: 1548 1548 Size: 6448 BfrBI ATG'CAT Cuts at: 3559 3559 Size: 6448 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5059 136 Size: 107 122 71 631 605 3387 1525 Fragments arranged by size: 3387 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6443 6443 Size: 6448 BlpI GC'TnA_GC Cuts at: 3865 3865 Size: 6448 Bme1580I G_kGCm'C Cuts at: 1790 2768 3211 4509 5665 1790 Size: 978 443 1298 1156 2573 Fragments arranged by size: 2573 1298 1156 978 443 BmgBI CAC'GTC Cuts at: 1318 3477 1318 Size: 2159 4289 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4179 5137 442 Size: 416 53 83 399 24 2762 958 1753 Fragments arranged by size: 2762 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6448 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3038 3038 Size: 6448 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2738 2866 3248 3335 5108 1239 Size: 1499 128 382 87 1773 2579 Fragments arranged by size: 2579 1773 1499 382 128 87 Bpu10I CC'TnA_GC Cuts at: 2324 2324 Size: 6448 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2504 4573 5438 5736 2504 Size: 2069 865 298 3216 Fragments arranged by size: 3216 2069 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2928 5111 882 Size: 617 1429 2183 2219 Fragments arranged by size: 2219 2183 1429 617 BsaAI yAC'GTr Cuts at: 493 1204 2862 4239 493 Size: 711 1658 1377 2702 Fragments arranged by size: 2702 1658 1377 711 BsaBI GATnn'nnATC Cuts at: 2315 6442 2315 Size: 4127 2321 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2651 4255 4637 5272 Size: 53 83 186 936 1118 1604 382 635 Cuts at: 5272 275 Size: 1451 Fragments arranged by size: 1604 1451 1118 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4881 5279 5553 5700 6111 4881 Size: 398 274 147 411 5218 Fragments arranged by size: 5218 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2051 2081 3284 3314 1178 Size: 30 165 30 648 30 1203 30 4312 Fragments arranged by size: 4312 1203 648 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2285 2303 2762 3027 1186 Size: 1099 18 459 265 4607 Fragments arranged by size: 4607 1099 459 265 18 BseYI C'CCAG_C Cuts at: 2101 2227 2644 5651 6147 2101 Size: 126 417 3007 496 2402 Fragments arranged by size: 3007 2402 496 417 126 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2288 2327 3171 3685 1163 Size: 573 552 39 844 514 3926 Fragments arranged by size: 3926 844 573 552 514 39 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2056 2207 2332 3898 4659 5687 Size: 275 186 55 151 125 1566 761 1028 Cuts at: 5687 1540 Size: 2301 Fragments arranged by size: 2301 1566 1028 761 275 186 151 125 55 BsiHKAI G_wGCw'C Cuts at: 2076 2550 3186 4509 4594 5665 2076 Size: 474 636 1323 85 1071 2859 Fragments arranged by size: 2859 1323 1071 636 474 85 BsmI GAATG_Cn' Cuts at: 3959 4052 3959 Size: 93 6355 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2108 2550 2638 2768 2826 3186 Size: 399 286 32 442 88 130 58 360 Cuts at: 3186 3211 3767 4509 4594 5665 1391 Size: 25 556 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 556 442 399 360 286 130 88 85 58 32 25 BspEI T'CCGG_A Cuts at: 6111 6111 Size: 6448 BspHI T'CATG_A Cuts at: 4337 6067 4337 Size: 1730 4718 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3803 844 Size: 2959 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4335 1394 Size: 429 2512 3507 Fragments arranged by size: 3507 2512 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3522 4943 5117 65 Size: 1183 2274 1421 174 1396 Fragments arranged by size: 2274 1421 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2191 2209 2215 5092 5279 1700 Size: 110 381 18 6 2877 187 2869 Fragments arranged by size: 2877 2869 381 187 110 18 6 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6448 BssHII G'CGCG_C Cuts at: 1925 2232 1925 Size: 307 6141 BssSI C'ACGA_G Cuts at: 4508 5520 4508 Size: 1012 5436 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6448 BstBI TT'CG_AA Cuts at: 2799 5252 2799 Size: 2453 3995 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6448 BstYI r'GATC_y Cuts at: 1972 2736 3039 3614 3778 4145 4530 4547 5999 Size: 764 303 575 164 367 385 17 1452 Cuts at: 5999 6085 6097 6443 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 764 575 385 367 346 303 164 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3321 3513 3321 Size: 192 6256 BtgI C'CryG_G Cuts at: 513 1065 2036 2201 513 Size: 552 971 165 4760 Fragments arranged by size: 4760 971 552 165 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2682 3179 487 Size: 683 56 1456 497 3756 Fragments arranged by size: 3756 1456 683 497 56 BtsI GCAGTG_nn' Cuts at: 2339 2763 3053 3964 4758 4778 2339 Size: 424 290 911 794 20 4009 Fragments arranged by size: 4009 911 794 424 290 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6448 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6413 DraI TTT'AAA Cuts at: 3178 3752 3178 Size: 574 5874 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5455 809 Size: 507 4139 1802 Fragments arranged by size: 4139 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2053 2094 2204 Size: 54 1425 50 275 18 223 41 110 Cuts at: 2204 2565 3088 3135 3895 4784 6413 8 Size: 361 523 47 760 889 1629 43 Fragments arranged by size: 1629 1425 889 760 523 361 275 223 110 54 50 47 43 41 18 EagI C'GGCC_G Cuts at: 1537 1812 2053 2204 3895 1537 Size: 275 241 151 1691 4090 Fragments arranged by size: 4090 1691 275 241 151 EarI CTCTTCn'nnn_ Cuts at: 1353 2739 4378 1353 Size: 1386 1639 3423 Fragments arranged by size: 3423 1639 1386 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5035 5409 5555 387 Size: 1064 138 3446 374 146 1280 Fragments arranged by size: 3446 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3184 3184 Size: 6448 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2573 2738 2866 3177 3248 3335 4511 Size: 393 941 165 128 311 71 87 1176 Cuts at: 4511 5108 5895 6163 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1176 941 787 597 393 311 268 165 128 87 71 EcoO109I rG'GnC_Cy Cuts at: 2156 2623 2156 Size: 467 5981 EcoRI G'AATT_C Cuts at: 3757 4135 3757 Size: 378 6070 EcoRV GAT'ATC Cuts at: 2500 2500 Size: 6448 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2537 2578 3304 3616 2537 Size: 41 726 312 5369 Fragments arranged by size: 5369 726 312 41 FspI TGC'GCA Cuts at: 4954 4954 Size: 6448 HaeII r_GCGC'y Cuts at: 1678 2032 2066 2084 2365 2563 5595 1678 Size: 354 34 18 281 198 3032 2531 Fragments arranged by size: 3032 2531 354 281 198 34 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2321 2353 3286 3318 3408 Size: 32 135 32 749 32 933 32 90 Cuts at: 3408 3440 3461 3493 3599 3631 5089 5121 5163 Size: 32 21 32 106 32 1458 32 42 Cuts at: 5163 5195 1373 Size: 32 2626 Fragments arranged by size: 2626 1458 933 749 135 106 90 42 32 32 32 32 32 32 32 32 32 21 HincII GTy'rAC Cuts at: 669 1657 1938 2454 3792 4038 669 Size: 988 281 516 1338 246 3079 Fragments arranged by size: 3079 1338 988 516 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6448 HpaI GTT'AAC Cuts at: 4038 4038 Size: 6448 KpnI G_GTAC'C Cuts at: 3773 3773 Size: 6448 MfeI C'AATT_G Cuts at: 4047 4047 Size: 6448 MluI A'CGCG_T Cuts at: 6324 6324 Size: 6448 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2951 5562 5746 1305 Size: 1646 2611 184 2007 Fragments arranged by size: 2611 2007 1646 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2567 3090 3137 10 Size: 54 1425 343 735 523 47 3321 Fragments arranged by size: 3321 1425 735 523 343 54 47 MslI CAynn'nnrTG Cuts at: 518 1166 3073 3527 4406 4765 4924 518 Size: 648 1907 454 879 359 159 2042 Fragments arranged by size: 2042 1907 879 648 454 359 159 MspA1I CmG'CkG Cuts at: 1606 2038 2203 2260 2374 2725 3155 3864 4541 Size: 432 165 57 114 351 430 709 677 Cuts at: 4541 5689 5934 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 709 677 432 430 351 245 165 114 57 NaeI GCC'GGC Cuts at: 2217 2217 Size: 6448 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5896 NdeI CA'TA_TG Cuts at: 387 387 Size: 6448 NgoMIV G'CCGG_C Cuts at: 2215 2215 Size: 6448 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6448 NotI GC'GGCC_GC Cuts at: 2204 3895 2204 Size: 1691 4757 NsiI A_TGCA'T Cuts at: 3561 3561 Size: 6448 NspI r_CATG'y Cuts at: 1132 1229 3317 3745 3806 5351 1132 Size: 97 2088 428 61 1545 2229 Fragments arranged by size: 2229 2088 1545 428 97 61 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6448 PciI A'CATG_T Cuts at: 5347 5347 Size: 6448 PflMI CCAn_nnn'nTGG Cuts at: 1108 3412 1108 Size: 2304 4144 PfoI T'CCnGG_A Cuts at: 2682 3252 2682 Size: 570 5878 PmeI GTTT'AAAC Cuts at: 3752 3752 Size: 6448 PmlI CAC'GTG Cuts at: 2862 2862 Size: 6448 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4517 4549 6030 6062 4517 Size: 32 1481 32 4903 Fragments arranged by size: 4903 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2156 2156 Size: 6448 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6448 PsiI TTA'TAA Cuts at: 2439 4018 2439 Size: 1579 4869 PstI C_TGCA'G Cuts at: 830 2259 2265 2286 2733 3800 830 Size: 1429 6 21 447 1067 3478 Fragments arranged by size: 3478 1429 1067 447 21 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6448 PvuII CAG'CTG Cuts at: 2260 3155 2260 Size: 895 5553 SacI G_AGCT'C Cuts at: 3186 3186 Size: 6448 SacII CC_GC'GG Cuts at: 2039 2204 2039 Size: 165 6283 SalI G'TCGA_C Cuts at: 1936 3790 1936 Size: 1854 4594 SanDI GG'GwC_CC Cuts at: 2156 2156 Size: 6448 SbfI CC_TGCA'GG Cuts at: 3800 3800 Size: 6448 ScaI AGT'ACT Cuts at: 4696 6408 4696 Size: 1712 4736 SexAI A'CCwGG_T Cuts at: 3420 3420 Size: 6448 SfcI C'TryA_G Cuts at: 826 1045 2255 2261 2282 2533 2729 3796 4931 Size: 219 1210 6 21 251 196 1067 1135 Cuts at: 4931 5612 5803 826 Size: 681 191 1471 Fragments arranged by size: 1471 1210 1135 1067 681 251 219 196 191 21 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6448 SgrAI Cr'CCGG_yG Cuts at: 1700 2209 1700 Size: 509 5939 SmaI CCC'GGG Cuts at: 1872 2179 3775 1872 Size: 307 1596 4545 Fragments arranged by size: 4545 1596 307 SmlI C'TyrA_G Cuts at: 820 1017 1943 2483 4552 5453 5715 820 Size: 197 926 540 2069 901 262 1553 Fragments arranged by size: 2069 1553 926 901 540 262 197 SnaBI TAC'GTA Cuts at: 493 4239 493 Size: 3746 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6448 SphI G_CATG'C Cuts at: 1132 3806 1132 Size: 2674 3774 SspI AAT'ATT Cuts at: 5 52 2966 4372 5 Size: 47 2914 1406 2081 Fragments arranged by size: 2914 2081 1406 47 StuI AGG'CCT Cuts at: 1517 3331 1517 Size: 1814 4634 StyI C'CwwG_G Cuts at: 513 1065 2589 3887 513 Size: 552 1524 1298 3074 Fragments arranged by size: 3074 1524 1298 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3087 3518 4475 4492 4645 4830 5275 Size: 1255 1507 431 957 17 153 185 445 Cuts at: 5275 325 Size: 1498 Fragments arranged by size: 1507 1498 1255 957 445 431 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3471 4694 6406 Size: 274 80 33 51 1443 1494 1223 1712 Cuts at: 6406 96 Size: 138 Fragments arranged by size: 1712 1494 1443 1223 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3062 3094 3617 3649 6408 6440 3062 Size: 32 523 32 2759 32 3070 Fragments arranged by size: 3070 2759 523 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6448 XbaI T'CTAG_A Cuts at: 3784 3784 Size: 6448 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6448 XmaI C'CCGG_G Cuts at: 1870 2177 3773 1870 Size: 307 1596 4545 Fragments arranged by size: 4545 1596 307 XmnI GAAnn'nnTTC Cuts at: 2795 3725 4577 2795 Size: 930 852 4666 Fragments arranged by size: 4666 930 852 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4256 276 Size: 53 83 186 936 2722 2468 Fragments arranged by size: 2722 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI FseI FspAI KasI NarI NruI PacI PspOMI PspXI PsrI RsrII SapI SfiI SfoI SrfI SwaI XcmI