################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AclI AcsI AgeI ApoI AscI AseI AsnI AvrII BbvCI BciVI BclI BfaI BglII BlnI BsaBI BsiWI BspDI BspLU11I BssHII Bst1107I BstAPI BstBI BstXI BstZ17I ClaI CpoI DraI DraIII EcoRI EcoRV EcoT22I FbaI HindIII HpaI MaeI MamI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PflMI PinAI PmeI PshBI Psp1406I PvuI RsrII SalI ScaI SfuI SgrAI SnaBI SpeI SspI SwaI Tsp509I Van91I XbaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 242 GGTACC G^GTACC AccI 2137 GTCTAC GT^[AC][GT]AC AflII 1918 CTTAAG C^TTAAG AflIII 2141 ACGTGT A^C[GA][CT]GT AhdI 2129 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 1879 AGCGCT AGC^GCT Asp718I 242 GGTACC G^GTACC AspEI 2129 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2083 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 674 TGCGCA TGC^GCA BbsI 1234 GAAGAC GAAGAC(2/6) BfrI 1918 CTTAAG C^TTAAG BlpI 661 GCTGAGC GC^T[ACGT]AGC Bpu1102I 661 GCTGAGC GC^T[ACGT]AGC BpuAI 1234 GAAGAC GAAGAC(2/6) Bsp1407I 3629 TGTACA T^GTACA BspHI 3992 TCATGA T^CATGA BsrGI 3629 TGTACA T^GTACA BstEII 165 GGTCACC G^GT[ACGT]ACC BstPI 165 GGTCACC G^GT[ACGT]ACC CelII 661 GCTGAGC GC^T[ACGT]AGC Eam1105I 2129 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1879 AGCGCT AGC^GCT EcoNI 708 CCTGGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 165 GGTCACC G^GT[ACGT]ACC FseI 2188 GGCCGGCC GGCCGG^CC FspI 674 TGCGCA TGC^GCA HgaI 769 GACGC GACGC(5/10) KpnI 242 GGTACC GGTAC^C MseI 1919 TTAA T^TAA NspI 3524 GCATGC [GA]CATG^[CT] PaeR7I 1457 CTCGAG C^TCGAG PflFI 2083 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 1288 GACGTAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 3992 TCATGA T^CATGA SphI 3524 GCATGC GCATG^C SspBI 3629 TGTACA T^GTACA Tru9I 1919 TTAA T^TAA Tth111I 2083 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1457 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### Alw44I 3118 GTGCAC G^TGCAC 3421 GTGCAC ApaLI 3118 GTGCAC G^TGCAC 3421 GTGCAC Asp700I 3186 GAACGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3984 GAACAGCTTC BbrPI 960 CACGTG CAC^GTG 2837 CACGTG BcgI 2313 CGACGGCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 755 GCAGCGGGCTCG BsmBI 793 GAGACG CGTCTC(1/5) 3865 GAGACG BsmI 554 GAATGC GAATGC(1/-1) 2510 GAATGC BsrBI 629 GAGCGG CCGCTC(-3/-3) 1743 GAGCGG BsrDI 2016 CATTGC GCAATG(2/0) 2573 GCAATG HincII 1866 GTCAAC GT[CT]^[GA]AC 3673 GTCAAC HindII 1866 GTCAAC GT[CT]^[GA]AC 3673 GTCAAC MslI 1404 CATTGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 3144 CATCTTCGTG PmaCI 960 CACGTG CAC^GTG 2837 CACGTG PmlI 960 CACGTG CAC^GTG 2837 CACGTG SacI 926 GAGCTC GAGCT^C 3166 GAGCTC SapI 3807 GAAGAGC GCTCTTC(1/4) 606 GCTCTTC StuI 1497 AGGCCT AGG^CCT 3902 AGGCCT TaqI 896 TCGA T^CGA 1458 TCGA TthHB8I 896 TCGA T^CGA 1458 TCGA XmnI 3186 GAACGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3984 GAACAGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 134 enzymes) # ######################################### AatII AccII AccIII AciI AcyI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BalI BamHI BanI BanII BbeI BbvI BcnI BglI BmyI BpmI BsaAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspEI BspMI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EagI EarI EclXI Eco52I Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV PleI PpuMI PstI PvuII RsaI SacII Sau3AI Sau96I SbfI ScrFI SexAI SfaNI SfcI SfiI SfoI SmaI SmlI Sse8387I StyI TfiI TseI Tsp45I TspRI XcmI XhoII XmaCI XmaI