(Circular) (Six-base) MAPSORT of: pFN21AB7280.seq Check: 3799 from: 1 to: 8713 pFN21AB7280 8713 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2504 5439 2504 Size: 2935 5778 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2196 3549 5787 6523 Size: 53 83 186 936 660 1353 2238 736 Cuts at: 6523 278 Size: 2468 Fragments arranged by size: 2468 2238 1353 936 736 660 186 83 53 AccI GT'mk_AC Cuts at: 1937 4143 6056 1937 Size: 2206 1913 4594 Fragments arranged by size: 4594 2206 1913 Acc65I G'GTAC_C Cuts at: 2247 6034 2247 Size: 3787 4926 AclI AA'CG_TT Cuts at: 1563 6840 7213 1563 Size: 5277 373 3063 Fragments arranged by size: 5277 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2693 3038 3370 4294 4694 5765 6776 8160 Size: 1061 345 332 924 400 1071 1011 1384 Cuts at: 8160 8428 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1071 1061 1011 924 400 345 332 268 AfeI AGC'GCT Cuts at: 3886 3886 Size: 8713 AflII C'TTAA_G Cuts at: 820 1017 3923 820 Size: 197 2906 5610 Fragments arranged by size: 5610 2906 197 AflIII A'CryG_T Cuts at: 1203 4146 7612 8589 1203 Size: 2943 3466 977 1327 Fragments arranged by size: 3466 2943 1327 977 AgeI A'CCGG_T Cuts at: 7544 7544 Size: 8713 AhdI GACnn_n'nnGTC Cuts at: 4139 7442 4139 Size: 3303 5410 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8713 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8713 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5705 5737 5705 Size: 32 8681 AlwNI CAG_nnn'CTG Cuts at: 2957 5632 5732 6456 8028 2957 Size: 2675 100 724 1572 3642 Fragments arranged by size: 3642 2675 1572 724 100 ApaI G_GGCC'C Cuts at: 2318 3053 3345 3372 3648 3801 3810 3864 4032 Size: 735 292 27 276 153 9 54 168 Cuts at: 4032 4337 4545 4637 4673 4952 4980 5036 5046 Size: 305 208 92 36 279 28 56 10 Cuts at: 5046 5055 2318 Size: 9 5976 Fragments arranged by size: 5976 735 305 292 279 276 208 168 153 92 56 54 36 28 27 10 9 9 ApaLI G'TGCA_C Cuts at: 5123 5426 6770 7926 5123 Size: 303 1344 1156 5910 Fragments arranged by size: 5910 1344 1156 303 ApoI r'AATT_y Cuts at: 1493 6022 6248 6400 8509 1493 Size: 4529 226 152 2109 1697 Fragments arranged by size: 4529 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 7267 160 Size: 872 6235 1606 Fragments arranged by size: 6235 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8713 AvaI C'yCGr_G Cuts at: 1870 1943 2081 2768 2778 3100 3278 3462 3596 Size: 73 138 687 10 322 178 184 134 Cuts at: 3596 3613 3989 4129 4270 4381 4445 4604 4784 Size: 17 376 140 141 111 64 159 180 Cuts at: 4784 5135 5381 5750 6038 1870 Size: 351 246 369 288 4545 Fragments arranged by size: 4545 687 376 369 351 322 288 246 184 180 178 159 141 140 138 134 111 73 64 17 10 BamHI G'GATC_C Cuts at: 3077 3931 4163 6043 6410 3077 Size: 854 232 1880 367 5380 Fragments arranged by size: 5380 1880 854 367 232 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2016 2043 2247 2397 Size: 325 217 627 221 8 27 204 150 Cuts at: 2397 2603 3200 3689 4325 4652 4941 5069 5594 Size: 206 597 489 636 327 289 128 525 Cuts at: 5594 5793 6034 7489 618 Size: 199 241 1455 1842 Fragments arranged by size: 1842 1455 636 627 597 525 489 327 325 289 241 221 217 206 204 199 150 128 27 8 BanII G_rGCy'C Cuts at: 1391 2318 2613 2702 2769 2935 3053 3345 3372 Size: 927 295 89 67 166 118 292 27 Cuts at: 3372 3648 3721 3766 3801 3810 3864 4032 4250 Size: 276 73 45 35 9 54 168 218 Cuts at: 4250 4337 4545 4637 4673 4785 4952 4980 5036 Size: 87 208 92 36 112 167 28 56 Cuts at: 5036 5046 5055 5175 5388 5407 6032 1391 Size: 10 9 120 213 19 625 4072 Fragments arranged by size: 4072 927 625 295 292 276 218 213 208 168 167 166 120 118 112 92 89 87 73 67 56 54 45 36 35 28 27 19 10 9 9 BbeI G_GCGC'C Cuts at: 2012 2047 2607 3693 4656 4945 5073 2012 Size: 35 560 1086 963 289 128 5652 Fragments arranged by size: 5652 1086 963 560 289 128 35 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3246 928 Size: 971 1347 6395 Fragments arranged by size: 6395 1347 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2749 2783 4307 4341 6904 6938 1903 Size: 34 812 34 1524 34 2563 34 3678 Fragments arranged by size: 3678 2563 1524 812 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6605 7815 6605 Size: 1210 7503 BclI T'GATC_A Cuts at: 1548 1548 Size: 8713 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2038 3057 3066 Size: 107 122 71 631 605 366 1019 9 Cuts at: 3066 3367 3510 3805 4008 4036 4113 4320 4818 Size: 301 143 295 203 28 77 207 498 Cuts at: 4818 4827 4836 4845 4896 4956 4965 5031 5050 Size: 9 9 9 51 60 9 66 19 Cuts at: 5050 7324 136 Size: 2274 1525 Fragments arranged by size: 2274 1525 1019 631 605 498 366 301 295 207 203 143 122 107 77 71 66 60 51 28 19 9 9 9 9 9 BglII A'GATC_T Cuts at: 8708 8708 Size: 8713 BlpI GC'TnA_GC Cuts at: 2667 6130 2667 Size: 3463 5250 Bme1580I G_kGCm'C Cuts at: 1790 2021 2318 2400 3053 3116 3345 3372 3648 Size: 231 297 82 653 63 229 27 276 Cuts at: 3648 3801 3810 3864 4032 4330 4337 4545 4637 Size: 153 9 54 168 298 7 208 92 Cuts at: 4637 4673 4952 4980 5036 5046 5055 5127 5430 Size: 36 279 28 56 10 9 72 303 Cuts at: 5430 5597 5673 5758 6774 7930 1790 Size: 167 76 85 1016 1156 2573 Fragments arranged by size: 2573 1156 1016 653 303 298 297 279 276 231 229 208 168 167 153 92 85 82 76 72 63 56 54 36 28 27 10 9 9 7 BmgBI CAC'GTC Cuts at: 1318 2056 5874 1318 Size: 738 3818 4157 Fragments arranged by size: 4157 3818 738 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3110 3669 6444 Size: 416 53 83 399 24 1693 559 2775 Cuts at: 6444 7402 442 Size: 958 1753 Fragments arranged by size: 2775 1753 1693 958 559 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8713 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5908 5908 Size: 8713 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2609 2615 2883 3270 3348 3464 4521 4539 Size: 1370 6 268 387 78 116 1057 18 Cuts at: 4539 4596 4623 4792 5234 7373 1239 Size: 57 27 169 442 2139 2579 Fragments arranged by size: 2579 2139 1370 1057 442 387 268 169 116 78 57 27 18 6 Bpu10I CC'TnA_GC Cuts at: 2622 4172 2622 Size: 1550 7163 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5669 6838 7703 8001 5669 Size: 1169 865 298 6381 Fragments arranged by size: 6381 1169 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3110 3312 4737 7376 882 Size: 617 1611 202 1425 2639 2219 Fragments arranged by size: 2639 2219 1611 1425 617 202 BsaAI yAC'GTr Cuts at: 493 1204 2536 2967 4147 4844 5425 6504 493 Size: 711 1332 431 1180 697 581 1079 2702 Fragments arranged by size: 2702 1332 1180 1079 711 697 581 431 BsaBI GATnn'nnATC Cuts at: 8707 8707 Size: 8713 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2044 2193 2604 Size: 53 83 186 936 476 35 149 411 Cuts at: 2604 2775 3546 3690 4653 4942 5070 5784 6520 Size: 171 771 144 963 289 128 714 736 Cuts at: 6520 6902 7537 275 Size: 382 635 1451 Fragments arranged by size: 1451 963 936 771 736 714 635 476 411 382 289 186 171 149 144 128 83 53 35 BsaWI w'CCGG_w Cuts at: 2747 2982 5199 7146 7544 7818 7965 8376 2747 Size: 235 2217 1947 398 274 147 411 3084 Fragments arranged by size: 3084 2217 1947 411 398 274 235 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2808 2838 3807 3837 5402 Size: 30 165 30 1405 30 969 30 1565 Cuts at: 5402 5432 5705 5735 5872 5902 1178 Size: 30 273 30 137 30 3989 Fragments arranged by size: 3989 1565 1405 969 273 165 137 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2138 2561 2810 2918 5065 5183 5902 1186 Size: 952 423 249 108 2147 118 719 3997 Fragments arranged by size: 3997 2147 952 719 423 249 118 108 BseYI C'CCAG_C Cuts at: 2163 2258 2365 3142 4025 5113 5388 5705 5709 Size: 95 107 777 883 1088 275 317 4 Cuts at: 5709 5920 7916 8412 2163 Size: 211 1996 496 2464 Fragments arranged by size: 2464 1996 1088 883 777 496 317 275 211 107 95 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2408 2454 5207 5270 5300 1163 Size: 573 672 46 2753 63 30 4576 Fragments arranged by size: 4576 2753 672 573 63 46 30 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4199 4400 4436 5438 5659 6163 Size: 275 186 2198 201 36 1002 221 504 Cuts at: 6163 6924 7952 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 2198 1028 1002 761 504 275 221 201 186 36 BsiHKAI G_wGCw'C Cuts at: 2061 2935 3739 5127 5175 5319 5430 5493 5979 Size: 874 804 1388 48 144 111 63 486 Cuts at: 5979 6774 6859 7930 2061 Size: 795 85 1071 2844 Fragments arranged by size: 2844 1388 1071 874 804 795 486 144 111 85 63 48 BsmI GAATG_Cn' Cuts at: 2565 4521 6224 6317 2565 Size: 1956 1703 93 4961 Fragments arranged by size: 4961 1956 1703 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2792 5864 2792 Size: 3072 5641 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2061 2318 2400 2613 2702 2769 Size: 399 231 40 257 82 213 89 67 Cuts at: 2769 2935 3053 3116 3345 3372 3648 3721 3739 Size: 166 118 63 229 27 276 73 18 Cuts at: 3739 3766 3801 3810 3864 4032 4250 4330 4337 Size: 27 35 9 54 168 218 80 7 Cuts at: 4337 4545 4637 4673 4785 4952 4980 5036 5046 Size: 208 92 36 112 167 28 56 10 Cuts at: 5046 5055 5127 5175 5319 5388 5407 5430 5493 Size: 9 72 48 144 69 19 23 63 Cuts at: 5493 5597 5673 5758 5979 6032 6774 6859 7930 Size: 104 76 85 221 53 742 85 1071 Cuts at: 7930 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 276 257 231 229 221 218 213 208 168 167 166 144 118 112 104 92 89 85 85 82 80 76 73 72 69 67 63 63 56 54 53 48 40 36 35 28 27 27 23 19 18 10 9 9 7 BspEI T'CCGG_A Cuts at: 2747 2982 5199 8376 2747 Size: 235 2217 3177 3084 Fragments arranged by size: 3177 3084 2217 235 BspHI T'CATG_A Cuts at: 5997 6602 8332 5997 Size: 605 1730 6378 Fragments arranged by size: 6378 1730 605 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2158 2504 3781 4231 5439 6068 844 Size: 1314 346 1277 450 1208 629 3489 Fragments arranged by size: 3489 1314 1277 1208 629 450 346 BsrBI CCG'CTC Cuts at: 1394 1823 2636 3750 6600 1394 Size: 429 813 1114 2850 3507 Fragments arranged by size: 3507 2850 1114 813 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4020 4585 7208 7382 65 Size: 1183 2772 565 2623 174 1396 Fragments arranged by size: 2772 2623 1396 1183 565 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3560 4194 5437 5485 7357 7544 1700 Size: 110 1750 634 1243 48 1872 187 2869 Fragments arranged by size: 2869 1872 1750 1243 634 187 110 48 BsrGI T'GTAC_A Cuts at: 96 5634 96 Size: 5538 3175 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8713 BssSI C'ACGA_G Cuts at: 3738 5126 5235 6773 7785 3738 Size: 1388 109 1538 1012 4666 Fragments arranged by size: 4666 1538 1388 1012 109 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8713 BstBI TT'CG_AA Cuts at: 7517 7517 Size: 8713 BstEII G'GTnAC_C Cuts at: 1184 2170 1184 Size: 986 7727 BstYI r'GATC_y Cuts at: 1972 3077 3422 3931 4075 4163 6043 6410 6795 Size: 1105 345 509 144 88 1880 367 385 Cuts at: 6795 6812 8264 8350 8362 8708 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1880 1452 1105 509 385 367 346 345 144 88 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4188 4911 5077 5789 4188 Size: 723 166 712 7112 Fragments arranged by size: 7112 723 712 166 BtgI C'CryG_G Cuts at: 513 1065 2004 2027 2033 2100 2355 2913 5064 Size: 552 939 23 6 67 255 558 2151 Cuts at: 5064 5274 5306 5542 5580 5898 513 Size: 210 32 236 38 318 3328 Fragments arranged by size: 3328 2151 939 558 552 318 255 236 210 67 38 32 23 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2742 487 Size: 683 56 1516 6458 Fragments arranged by size: 6458 1516 683 56 BtsI GCAGTG_nn' Cuts at: 2282 2707 6229 7023 7043 2282 Size: 425 3522 794 20 3952 Fragments arranged by size: 3952 3522 794 425 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8713 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8678 DraI TTT'AAA Cuts at: 6017 6017 Size: 8713 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2192 2978 4166 4383 4437 7720 809 Size: 507 876 786 1188 217 54 3283 1802 Fragments arranged by size: 3283 1802 1188 876 786 507 217 54 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2024 2030 2233 Size: 54 1425 50 275 18 194 6 203 Cuts at: 2233 2300 2482 3058 4196 4397 4433 5157 5256 Size: 67 182 576 1138 201 36 724 99 Cuts at: 5256 5361 5435 5895 6160 7049 8678 8 Size: 105 74 460 265 889 1629 43 Fragments arranged by size: 1629 1425 1138 889 724 576 460 275 265 203 201 194 182 105 99 74 67 54 50 43 36 18 6 EagI C'GGCC_G Cuts at: 1537 1812 4196 4397 4433 5435 6160 1537 Size: 275 2384 201 36 1002 725 4090 Fragments arranged by size: 4090 2384 1002 725 275 201 36 EarI CTCTTCn'nnn_ Cuts at: 1353 2270 2618 3631 5807 6643 1353 Size: 917 348 1013 2176 836 3423 Fragments arranged by size: 3423 2176 1013 917 836 348 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5893 7300 7674 7820 387 Size: 1064 138 4304 1407 374 146 1280 Fragments arranged by size: 4304 1407 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2933 5173 2933 Size: 2240 6473 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2609 2615 2693 2883 3038 3270 3348 Size: 393 977 6 78 190 155 232 78 Cuts at: 3348 3370 3464 4294 4521 4539 4596 4623 4694 Size: 22 94 830 227 18 57 27 71 Cuts at: 4694 4792 5234 5765 6776 7373 8160 8428 1239 Size: 98 442 531 1011 597 787 268 1524 Fragments arranged by size: 1524 1011 977 830 787 597 531 442 393 268 232 227 190 155 98 94 78 78 71 57 27 22 18 6 EcoNI CCTnn'n_nnAGG Cuts at: 2717 2717 Size: 8713 EcoO109I rG'GnC_Cy Cuts at: 2152 2314 2584 2686 3068 3131 3173 3182 3299 Size: 162 270 102 382 63 42 9 117 Cuts at: 3299 3341 3342 3350 3359 3368 3369 3377 3448 Size: 42 1 8 9 9 1 8 71 Cuts at: 3448 3458 3554 3590 3617 3626 3644 3645 3788 Size: 10 96 36 27 9 18 1 143 Cuts at: 3788 3798 3807 3860 3861 3902 4029 4133 4184 Size: 10 9 53 1 41 127 104 51 Cuts at: 4184 4229 4274 4334 4376 4532 4541 4542 4550 Size: 45 45 60 42 156 9 1 8 Cuts at: 4550 4633 4634 4643 4670 4865 4916 4948 4949 Size: 83 1 9 27 195 51 32 1 Cuts at: 4949 4967 4976 4977 5033 5043 5052 5629 2152 Size: 18 9 1 56 10 9 577 5236 Fragments arranged by size: 5236 577 382 270 195 162 156 143 127 117 104 102 96 83 71 63 60 56 53 51 51 45 45 42 42 42 41 36 32 27 27 18 18 10 10 10 9 9 9 9 9 9 9 9 9 8 8 8 1 1 1 1 1 1 1 1 EcoRI G'AATT_C Cuts at: 6022 6400 6022 Size: 378 8335 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3421 3421 Size: 8713 FseI GG_CCGG'CC Cuts at: 4198 4198 Size: 8713 FspI TGC'GCA Cuts at: 2681 7219 2681 Size: 4538 4175 HaeII r_GCGC'y Cuts at: 1678 2012 2047 2607 3693 3888 4656 4945 5073 Size: 334 35 560 1086 195 768 289 128 Cuts at: 5073 7860 1678 Size: 2787 2531 Fragments arranged by size: 2787 2531 1086 768 560 334 289 195 128 35 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2729 2761 2945 2977 3807 Size: 32 135 32 1157 32 184 32 830 Cuts at: 3807 3839 4125 4157 4849 4866 4881 4898 5872 Size: 32 286 32 692 17 15 17 974 Cuts at: 5872 5904 5944 5976 7354 7386 7428 7460 1373 Size: 32 40 32 1378 32 42 32 2626 Fragments arranged by size: 2626 1378 1157 974 830 692 286 184 135 42 40 32 32 32 32 32 32 32 32 32 32 17 17 15 HincII GTy'rAC Cuts at: 669 1657 1938 3873 5680 6057 6303 669 Size: 988 281 1935 1807 377 246 3079 Fragments arranged by size: 3079 1935 1807 988 377 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8713 HpaI GTT'AAC Cuts at: 6303 6303 Size: 8713 KasI G'GCGC_C Cuts at: 2008 2043 2603 3689 4652 4941 5069 2008 Size: 35 560 1086 963 289 128 5652 Fragments arranged by size: 5652 1086 963 560 289 128 35 KpnI G_GTAC'C Cuts at: 2251 6038 2251 Size: 3787 4926 MfeI C'AATT_G Cuts at: 6312 6312 Size: 8713 MluI A'CGCG_T Cuts at: 8589 8589 Size: 8713 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3310 3567 3952 4131 4695 7827 8011 1305 Size: 2005 257 385 179 564 3132 184 2007 Fragments arranged by size: 3132 2007 2005 564 385 257 184 179 MscI TGG'CCA Cuts at: 10 64 1489 1832 2026 2235 5363 10 Size: 54 1425 343 194 209 3128 3360 Fragments arranged by size: 3360 3128 1425 343 209 194 54 MslI CAynn'nnrTG Cuts at: 518 1166 3413 5153 6671 7030 7189 518 Size: 648 2247 1740 1518 359 159 2042 Fragments arranged by size: 2247 2042 1740 1518 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2035 2102 2763 3830 5212 5284 5308 5544 Size: 429 67 661 1067 1382 72 24 236 Cuts at: 5544 5709 5743 6129 6806 7954 8199 1606 Size: 165 34 386 677 1148 245 2120 Fragments arranged by size: 2120 1382 1148 1067 677 661 429 386 245 236 165 72 67 34 24 NaeI GCC'GGC Cuts at: 3562 4196 5439 3562 Size: 634 1243 6836 Fragments arranged by size: 6836 1243 634 NarI GG'CG_CC Cuts at: 2009 2044 2604 3690 4653 4942 5070 2009 Size: 35 560 1086 963 289 128 5652 Fragments arranged by size: 5652 1086 963 560 289 128 35 NcoI C'CATG_G Cuts at: 513 1065 2004 2027 2355 5064 513 Size: 552 939 23 328 2709 4162 Fragments arranged by size: 4162 2709 939 552 328 23 NdeI CA'TA_TG Cuts at: 387 387 Size: 8713 NgoMIV G'CCGG_C Cuts at: 3560 4194 5437 3560 Size: 634 1243 6836 Fragments arranged by size: 6836 1243 634 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8713 NotI GC'GGCC_GC Cuts at: 6160 6160 Size: 8713 NspI r_CATG'y Cuts at: 1132 1229 5533 6071 7616 1132 Size: 97 4304 538 1545 2229 Fragments arranged by size: 4304 2229 1545 538 97 PasI CC'CwG_GG Cuts at: 1348 2318 2990 3127 3179 3323 3794 3803 4033 Size: 970 672 137 52 144 471 9 230 Cuts at: 4033 4330 4853 4973 5039 5048 1348 Size: 297 523 120 66 9 5013 Fragments arranged by size: 5013 970 672 523 471 297 230 144 137 120 66 52 9 9 PciI A'CATG_T Cuts at: 7612 7612 Size: 8713 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8713 PfoI T'CCnGG_A Cuts at: 2049 3481 3631 4216 4426 4612 4639 2049 Size: 1432 150 585 210 186 27 6123 Fragments arranged by size: 6123 1432 585 210 186 150 27 PmeI GTTT'AAAC Cuts at: 6017 6017 Size: 8713 PmlI CAC'GTG Cuts at: 2967 4844 2967 Size: 1877 6836 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5705 5737 6782 6814 8295 8327 5705 Size: 32 1045 32 1481 32 6091 Fragments arranged by size: 6091 1481 1045 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2152 2686 3131 3173 3299 3350 3377 3458 3554 Size: 534 445 42 126 51 27 81 96 Cuts at: 3554 3590 3617 3902 4133 4184 4229 4274 4376 Size: 36 27 285 231 51 45 45 102 Cuts at: 4376 4532 4643 4865 4916 5629 2152 Size: 156 111 222 51 713 5236 Fragments arranged by size: 5236 713 534 445 285 231 222 156 126 111 102 96 81 51 51 51 45 45 42 36 27 27 PshAI GACnn'nnGTC Cuts at: 1613 3297 1613 Size: 1684 7029 PsiI TTA'TAA Cuts at: 6283 6283 Size: 8713 PspOMI G'GGCC_C Cuts at: 2314 3049 3341 3368 3644 3797 3806 3860 4028 Size: 735 292 27 276 153 9 54 168 Cuts at: 4028 4333 4541 4633 4669 4948 4976 5032 5042 Size: 305 208 92 36 279 28 56 10 Cuts at: 5042 5051 2314 Size: 9 5976 Fragments arranged by size: 5976 735 305 292 279 276 208 168 153 92 56 54 36 28 27 10 9 9 PspXI vC'TCGA_Gb Cuts at: 3462 3462 Size: 8713 PstI C_TGCA'G Cuts at: 830 2142 3139 4228 5628 6065 830 Size: 1312 997 1089 1400 437 3478 Fragments arranged by size: 3478 1400 1312 1089 997 437 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8713 PvuII CAG'CTG Cuts at: 5212 5284 5709 5212 Size: 72 425 8216 Fragments arranged by size: 8216 425 72 SacI G_AGCT'C Cuts at: 2935 5175 2935 Size: 2240 6473 SacII CC_GC'GG Cuts at: 2036 2103 5309 5545 2036 Size: 67 3206 236 5204 Fragments arranged by size: 5204 3206 236 67 SalI G'TCGA_C Cuts at: 1936 6055 1936 Size: 4119 4594 SanDI GG'GwC_CC Cuts at: 2152 2686 3590 3617 4274 4916 2152 Size: 534 904 27 657 642 5949 Fragments arranged by size: 5949 904 657 642 534 27 SapI GCTCTTCn'nnn_ Cuts at: 2618 5807 2618 Size: 3189 5524 SbfI CC_TGCA'GG Cuts at: 3139 4228 5628 6065 3139 Size: 1089 1400 437 5787 Fragments arranged by size: 5787 1400 1089 437 ScaI AGT'ACT Cuts at: 6961 8673 6961 Size: 1712 7001 SexAI A'CCwGG_T Cuts at: 2428 3091 5496 2428 Size: 663 2405 5645 Fragments arranged by size: 5645 2405 663 SfcI C'TryA_G Cuts at: 826 1045 2138 3135 4224 5624 6061 7196 7877 Size: 219 1093 997 1089 1400 437 1135 681 Cuts at: 7877 8068 826 Size: 191 1471 Fragments arranged by size: 1471 1400 1135 1093 1089 997 681 437 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3057 3066 3367 3805 4036 4113 4827 4836 4896 Size: 9 301 438 231 77 714 9 60 Cuts at: 4896 4956 4965 5031 5050 3057 Size: 60 9 66 19 6720 Fragments arranged by size: 6720 714 438 301 231 77 66 60 60 19 9 9 9 SfoI GGC'GCC Cuts at: 2010 2045 2605 3691 4654 4943 5071 2010 Size: 35 560 1086 963 289 128 5652 Fragments arranged by size: 5652 1086 963 560 289 128 35 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8713 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8713 SmaI CCC'GGG Cuts at: 1872 2780 3102 3280 3598 3615 3991 4131 4272 Size: 908 322 178 318 17 376 140 141 Cuts at: 4272 4383 4447 4606 4786 5752 6040 1872 Size: 111 64 159 180 966 288 4545 Fragments arranged by size: 4545 966 908 376 322 318 288 180 178 159 141 140 111 64 17 SmlI C'TyrA_G Cuts at: 820 1017 1943 3462 3923 5684 6817 7718 7980 Size: 197 926 1519 461 1761 1133 901 262 Cuts at: 7980 820 Size: 1553 Fragments arranged by size: 1761 1553 1519 1133 926 901 461 262 197 SnaBI TAC'GTA Cuts at: 493 6504 493 Size: 6011 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8713 SphI G_CATG'C Cuts at: 1132 5533 6071 1132 Size: 4401 538 3774 Fragments arranged by size: 4401 3774 538 SspI AAT'ATT Cuts at: 5 52 6637 5 Size: 47 6585 2081 Fragments arranged by size: 6585 2081 47 StuI AGG'CCT Cuts at: 1517 3504 5909 1517 Size: 1987 2405 4321 Fragments arranged by size: 4321 2405 1987 StyI C'CwwG_G Cuts at: 513 1065 2004 2027 2146 2355 2856 3303 3355 Size: 552 939 23 119 209 501 447 52 Cuts at: 3355 4233 4278 4353 4495 4647 4869 5064 6009 Size: 878 45 75 142 152 222 195 945 Cuts at: 6009 6152 513 Size: 143 3074 Fragments arranged by size: 3074 945 939 878 552 501 447 222 209 195 152 143 142 119 75 52 45 23 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2189 2562 3668 4761 5870 6740 6757 Size: 1255 609 373 1106 1093 1109 870 17 Cuts at: 6757 6910 7095 7540 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1109 1106 1093 870 609 445 373 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5634 6959 8671 Size: 274 80 33 51 1443 3657 1325 1712 Cuts at: 8671 96 Size: 138 Fragments arranged by size: 3657 1712 1443 1325 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2274 2306 3258 3290 5402 5434 8673 8705 2274 Size: 32 952 32 2112 32 3239 32 2282 Fragments arranged by size: 3239 2282 2112 952 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4091 1316 Size: 2775 5938 XbaI T'CTAG_A Cuts at: 6049 6049 Size: 8713 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2362 2710 2854 4240 4663 2362 Size: 348 144 1386 423 6412 Fragments arranged by size: 6412 1386 423 348 144 XhoI C'TCGA_G Cuts at: 1943 3462 1943 Size: 1519 7194 XmaI C'CCGG_G Cuts at: 1870 2778 3100 3278 3596 3613 3989 4129 4270 Size: 908 322 178 318 17 376 140 141 Cuts at: 4270 4381 4445 4604 4784 5750 6038 1870 Size: 111 64 159 180 966 288 4545 Fragments arranged by size: 4545 966 908 376 322 318 288 180 178 159 141 140 111 64 17 XmnI GAAnn'nnTTC Cuts at: 5195 5993 6842 5195 Size: 798 849 7066 Fragments arranged by size: 7066 849 798 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2194 3547 5785 6521 Size: 53 83 186 936 660 1353 2238 736 Cuts at: 6521 276 Size: 2468 Fragments arranged by size: 2468 2238 1353 936 736 660 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbvCI BfrBI BsiWI BstXI BstZ17I DraIII EcoRV FspAI NruI NsiI PacI PsrI RsrII SrfI SwaI