(Linear) (Six-base) MAPSORT of: KIBB7280.seq Check: 9325 from: 1 to: 4008 KIBB7280.orf With 182 enzymes: SgfI * July 15, 2010 18:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 499 3434 4008 Size: 499 2935 574 Fragments arranged by size: 2935 574 499 AatII G_ACGT'C Cuts at: 0 191 1544 3782 4008 Size: 191 1353 2238 226 Fragments arranged by size: 2238 1353 226 191 AccI GT'mk_AC Cuts at: 0 2138 4008 Size: 2138 1870 Acc65I G'GTAC_C Cuts at: 0 242 4008 Size: 242 3766 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 688 1033 1365 2289 2689 3760 4008 Size: 688 345 332 924 400 1071 248 Fragments arranged by size: 1071 924 688 400 345 332 248 AfeI AGC'GCT Cuts at: 0 1881 4008 Size: 1881 2127 AflII C'TTAA_G Cuts at: 0 1918 4008 Size: 1918 2090 AflIII A'CryG_T Cuts at: 0 2141 4008 Size: 2141 1867 AhdI GACnn_n'nnGTC Cuts at: 0 2134 4008 Size: 2134 1874 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 3700 3732 4008 Size: 3700 32 276 Fragments arranged by size: 3700 276 32 AlwNI CAG_nnn'CTG Cuts at: 0 952 3627 3727 4008 Size: 952 2675 100 281 Fragments arranged by size: 2675 952 281 100 ApaI G_GGCC'C Cuts at: 0 313 1048 1340 1367 1643 1796 1805 1859 Size: 313 735 292 27 276 153 9 54 Cuts at: 1859 2027 2332 2540 2632 2668 2947 2975 3031 Size: 168 305 208 92 36 279 28 56 Cuts at: 3031 3041 3050 4008 Size: 10 9 958 Fragments arranged by size: 958 735 313 305 292 279 276 208 168 153 92 56 54 36 28 27 10 9 9 ApaLI G'TGCA_C Cuts at: 0 3118 3421 4008 Size: 3118 303 587 Fragments arranged by size: 3118 587 303 AvaI C'yCGr_G Cuts at: 0 76 763 773 1095 1273 1457 1591 1608 Size: 76 687 10 322 178 184 134 17 Cuts at: 1608 1984 2124 2265 2376 2440 2599 2779 3130 Size: 376 140 141 111 64 159 180 351 Cuts at: 3130 3376 3745 4008 Size: 246 369 263 Fragments arranged by size: 687 376 369 351 322 263 246 184 180 178 159 141 140 134 111 76 64 17 10 BamHI G'GATC_C Cuts at: 0 1072 1926 2158 4008 Size: 1072 854 232 1850 Fragments arranged by size: 1850 1072 854 232 BanI G'GyrC_C Cuts at: 0 3 11 38 242 392 598 1195 1684 Size: 3 8 27 204 150 206 597 489 Cuts at: 1684 2320 2647 2936 3064 3589 3788 4008 Size: 636 327 289 128 525 199 220 Fragments arranged by size: 636 597 525 489 327 289 220 206 204 199 150 128 27 8 3 BanII G_rGCy'C Cuts at: 0 313 608 697 764 930 1048 1340 1367 Size: 313 295 89 67 166 118 292 27 Cuts at: 1367 1643 1716 1761 1796 1805 1859 2027 2245 Size: 276 73 45 35 9 54 168 218 Cuts at: 2245 2332 2540 2632 2668 2780 2947 2975 3031 Size: 87 208 92 36 112 167 28 56 Cuts at: 3031 3041 3050 3170 3383 3402 4008 Size: 10 9 120 213 19 606 Fragments arranged by size: 606 313 295 292 276 218 213 208 168 167 166 120 118 112 92 89 87 73 67 56 54 45 36 35 28 27 19 10 9 9 BbeI G_GCGC'C Cuts at: 0 7 42 602 1688 2651 2940 3068 4008 Size: 7 35 560 1086 963 289 128 940 Fragments arranged by size: 1086 963 940 560 289 128 35 7 BbsI GAAGACnn'nnnn_ Cuts at: 0 1241 4008 Size: 1241 2767 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 744 778 2302 2336 4008 Size: 744 34 1524 34 1672 Fragments arranged by size: 1672 1524 744 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 33 1052 1061 1362 1505 1800 2003 2031 Size: 33 1019 9 301 143 295 203 28 Cuts at: 2031 2108 2315 2813 2822 2831 2840 2891 2951 Size: 77 207 498 9 9 9 51 60 Cuts at: 2951 2960 3026 3045 4008 Size: 9 66 19 963 Fragments arranged by size: 1019 963 498 301 295 207 203 143 77 66 60 51 33 28 19 9 9 9 9 9 BlpI GC'TnA_GC Cuts at: 0 662 4008 Size: 662 3346 Bme1580I G_kGCm'C Cuts at: 0 16 313 395 1048 1111 1340 1367 1643 Size: 16 297 82 653 63 229 27 276 Cuts at: 1643 1796 1805 1859 2027 2325 2332 2540 2632 Size: 153 9 54 168 298 7 208 92 Cuts at: 2632 2668 2947 2975 3031 3041 3050 3122 3425 Size: 36 279 28 56 10 9 72 303 Cuts at: 3425 3592 3668 3753 4008 Size: 167 76 85 255 Fragments arranged by size: 653 303 298 297 279 276 255 229 208 168 167 153 92 85 82 76 72 63 56 54 36 28 27 16 10 9 9 7 BmgBI CAC'GTC Cuts at: 0 51 3869 4008 Size: 51 3818 139 Fragments arranged by size: 3818 139 51 BmrI ACTGGGnnnn_n' Cuts at: 0 1105 1664 4008 Size: 1105 559 2344 Fragments arranged by size: 2344 1105 559 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 3903 4008 Size: 3903 105 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 604 610 878 1265 1343 1459 2516 2534 Size: 604 6 268 387 78 116 1057 18 Cuts at: 2534 2591 2618 2787 3229 4008 Size: 57 27 169 442 779 Fragments arranged by size: 1057 779 604 442 387 268 169 116 78 57 27 18 6 Bpu10I CC'TnA_GC Cuts at: 0 617 2167 4008 Size: 617 1550 1841 Fragments arranged by size: 1841 1550 617 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 3664 4008 Size: 3664 344 BsaI GGTCTCn'nnnn_ Cuts at: 0 1105 1307 2732 4008 Size: 1105 202 1425 1276 Fragments arranged by size: 1425 1276 1105 202 BsaAI yAC'GTr Cuts at: 0 531 962 2142 2839 3420 4008 Size: 531 431 1180 697 581 588 Fragments arranged by size: 1180 697 588 581 531 431 BsaHI Gr'CG_yC Cuts at: 0 4 39 188 599 770 1541 1685 2648 Size: 4 35 149 411 171 771 144 963 Cuts at: 2648 2937 3065 3779 4008 Size: 289 128 714 229 Fragments arranged by size: 963 771 714 411 289 229 171 149 144 128 35 4 BsaWI w'CCGG_w Cuts at: 0 742 977 3194 4008 Size: 742 235 2217 814 Fragments arranged by size: 2217 814 742 235 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 803 833 1802 1832 3397 3427 3700 3730 Size: 803 30 969 30 1565 30 273 30 Cuts at: 3730 3867 3897 4008 Size: 137 30 111 Fragments arranged by size: 1565 969 803 273 137 111 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 133 556 805 913 3060 3178 3897 4008 Size: 133 423 249 108 2147 118 719 111 Fragments arranged by size: 2147 719 423 249 133 118 111 108 BseYI C'CCAG_C Cuts at: 0 158 253 360 1137 2020 3108 3383 3700 Size: 158 95 107 777 883 1088 275 317 Cuts at: 3700 3704 3915 4008 Size: 4 211 93 Fragments arranged by size: 1088 883 777 317 275 211 158 107 95 93 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 403 449 3202 3265 3295 4008 Size: 403 46 2753 63 30 713 Fragments arranged by size: 2753 713 403 63 46 30 BsiEI CG_ry'CG Cuts at: 0 2194 2395 2431 3433 3654 4008 Size: 2194 201 36 1002 221 354 Fragments arranged by size: 2194 1002 354 221 201 36 BsiHKAI G_wGCw'C Cuts at: 0 56 930 1734 3122 3170 3314 3425 3488 Size: 56 874 804 1388 48 144 111 63 Cuts at: 3488 3974 4008 Size: 486 34 Fragments arranged by size: 1388 874 804 486 144 111 63 56 48 34 BsmI GAATG_Cn' Cuts at: 0 560 2516 4008 Size: 560 1956 1492 Fragments arranged by size: 1956 1492 560 BsmBI CGTCTCn'nnnn_ Cuts at: 0 787 3859 4008 Size: 787 3072 149 Fragments arranged by size: 3072 787 149 Bsp1286I G_dGCh'C Cuts at: 0 16 56 313 395 608 697 764 930 Size: 16 40 257 82 213 89 67 166 Cuts at: 930 1048 1111 1340 1367 1643 1716 1734 1761 Size: 118 63 229 27 276 73 18 27 Cuts at: 1761 1796 1805 1859 2027 2245 2325 2332 2540 Size: 35 9 54 168 218 80 7 208 Cuts at: 2540 2632 2668 2780 2947 2975 3031 3041 3050 Size: 92 36 112 167 28 56 10 9 Cuts at: 3050 3122 3170 3314 3383 3402 3425 3488 3592 Size: 72 48 144 69 19 23 63 104 Cuts at: 3592 3668 3753 3974 4008 Size: 76 85 221 34 Fragments arranged by size: 276 257 229 221 218 213 208 168 167 166 144 118 112 104 92 89 85 82 80 76 73 72 69 67 63 63 56 54 48 40 36 35 34 28 27 27 23 19 18 16 10 9 9 7 BspEI T'CCGG_A Cuts at: 0 742 977 3194 4008 Size: 742 235 2217 814 Fragments arranged by size: 2217 814 742 235 BspHI T'CATG_A Cuts at: 0 3992 4008 Size: 3992 16 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 153 499 1776 2226 3434 4008 Size: 153 346 1277 450 1208 574 Fragments arranged by size: 1277 1208 574 450 346 153 BsrBI CCG'CTC Cuts at: 0 631 1745 4008 Size: 631 1114 2263 Fragments arranged by size: 2263 1114 631 BsrDI GCAATG_nn' Cuts at: 0 2015 2580 4008 Size: 2015 565 1428 Fragments arranged by size: 2015 1428 565 BsrFI r'CCGG_y Cuts at: 0 1555 2189 3432 3480 4008 Size: 1555 634 1243 48 528 Fragments arranged by size: 1555 1243 634 528 48 BsrGI T'GTAC_A Cuts at: 0 3629 4008 Size: 3629 379 BssSI C'ACGA_G Cuts at: 0 1733 3121 3230 4008 Size: 1733 1388 109 778 Fragments arranged by size: 1733 1388 778 109 BstEII G'GTnAC_C Cuts at: 0 165 4008 Size: 165 3843 BstYI r'GATC_y Cuts at: 0 1072 1417 1926 2070 2158 4008 Size: 1072 345 509 144 88 1850 Fragments arranged by size: 1850 1072 509 345 144 88 Bsu36I CC'TnA_GG Cuts at: 0 2183 2906 3072 3784 4008 Size: 2183 723 166 712 224 Fragments arranged by size: 2183 723 712 224 166 BtgI C'CryG_G Cuts at: 0 22 28 95 350 908 3059 3269 3301 Size: 22 6 67 255 558 2151 210 32 Cuts at: 3301 3537 3575 3893 4008 Size: 236 38 318 115 Fragments arranged by size: 2151 558 318 255 236 210 115 67 38 32 22 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 737 4008 Size: 737 3271 BtsI GCAGTG_nn' Cuts at: 0 277 702 4008 Size: 277 425 3306 Fragments arranged by size: 3306 425 277 DrdI GACnn_nn'nnGTC Cuts at: 0 187 973 2161 2378 2432 4008 Size: 187 786 1188 217 54 1576 Fragments arranged by size: 1576 1188 786 217 187 54 EaeI y'GGCC_r Cuts at: 0 19 25 228 295 477 1053 2191 2392 Size: 19 6 203 67 182 576 1138 201 Cuts at: 2392 2428 3152 3251 3356 3430 3890 4008 Size: 36 724 99 105 74 460 118 Fragments arranged by size: 1138 724 576 460 203 201 182 118 105 99 74 67 36 19 6 EagI C'GGCC_G Cuts at: 0 2191 2392 2428 3430 4008 Size: 2191 201 36 1002 578 Fragments arranged by size: 2191 1002 578 201 36 EarI CTCTTCn'nnn_ Cuts at: 0 265 613 1626 3802 4008 Size: 265 348 1013 2176 206 Fragments arranged by size: 2176 1013 348 265 206 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 3888 4008 Size: 3888 120 EcoICRI GAG'CTC Cuts at: 0 928 3168 4008 Size: 928 2240 840 Fragments arranged by size: 2240 928 840 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 604 610 688 878 1033 1265 1343 1365 Size: 604 6 78 190 155 232 78 22 Cuts at: 1365 1459 2289 2516 2534 2591 2618 2689 2787 Size: 94 830 227 18 57 27 71 98 Cuts at: 2787 3229 3760 4008 Size: 442 531 248 Fragments arranged by size: 830 604 531 442 248 232 227 190 155 98 94 78 78 71 57 27 22 18 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 712 4008 Size: 712 3296 EcoO109I rG'GnC_Cy Cuts at: 0 147 309 579 681 1063 1126 1168 1177 Size: 147 162 270 102 382 63 42 9 Cuts at: 1177 1294 1336 1337 1345 1354 1363 1364 1372 Size: 117 42 1 8 9 9 1 8 Cuts at: 1372 1443 1453 1549 1585 1612 1621 1639 1640 Size: 71 10 96 36 27 9 18 1 Cuts at: 1640 1783 1793 1802 1855 1856 1897 2024 2128 Size: 143 10 9 53 1 41 127 104 Cuts at: 2128 2179 2224 2269 2329 2371 2527 2536 2537 Size: 51 45 45 60 42 156 9 1 Cuts at: 2537 2545 2628 2629 2638 2665 2860 2911 2943 Size: 8 83 1 9 27 195 51 32 Cuts at: 2943 2944 2962 2971 2972 3028 3038 3047 3624 Size: 1 18 9 1 56 10 9 577 Cuts at: 3624 4008 Size: 384 Fragments arranged by size: 577 384 382 270 195 162 156 147 143 127 117 104 102 96 83 71 63 60 56 53 51 51 45 45 42 42 42 41 36 32 27 27 18 18 10 10 10 9 9 9 9 9 9 9 9 9 8 8 8 1 1 1 1 1 1 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1416 4008 Size: 1416 2592 FseI GG_CCGG'CC Cuts at: 0 2193 4008 Size: 2193 1815 FspI TGC'GCA Cuts at: 0 676 4008 Size: 676 3332 HaeII r_GCGC'y Cuts at: 0 7 42 602 1688 1883 2651 2940 3068 Size: 7 35 560 1086 195 768 289 128 Cuts at: 3068 4008 Size: 940 Fragments arranged by size: 1086 940 768 560 289 195 128 35 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 724 756 940 972 1802 1834 2120 2152 Size: 724 32 184 32 830 32 286 32 Cuts at: 2152 2844 2861 2876 2893 3867 3899 3939 3971 Size: 692 17 15 17 974 32 40 32 Cuts at: 3971 4008 Size: 37 Fragments arranged by size: 974 830 724 692 286 184 40 37 32 32 32 32 32 32 17 17 15 HincII GTy'rAC Cuts at: 0 1868 3675 4008 Size: 1868 1807 333 Fragments arranged by size: 1868 1807 333 KasI G'GCGC_C Cuts at: 0 3 38 598 1684 2647 2936 3064 4008 Size: 3 35 560 1086 963 289 128 944 Fragments arranged by size: 1086 963 944 560 289 128 35 3 KpnI G_GTAC'C Cuts at: 0 246 4008 Size: 246 3762 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1305 1562 1947 2126 2690 4008 Size: 1305 257 385 179 564 1318 Fragments arranged by size: 1318 1305 564 385 257 179 MscI TGG'CCA Cuts at: 0 21 230 3358 4008 Size: 21 209 3128 650 Fragments arranged by size: 3128 650 209 21 MslI CAynn'nnrTG Cuts at: 0 1408 3148 4008 Size: 1408 1740 860 Fragments arranged by size: 1740 1408 860 MspA1I CmG'CkG Cuts at: 0 30 97 758 1825 3207 3279 3303 3539 Size: 30 67 661 1067 1382 72 24 236 Cuts at: 3539 3704 3738 4008 Size: 165 34 270 Fragments arranged by size: 1382 1067 661 270 236 165 72 67 34 30 24 NaeI GCC'GGC Cuts at: 0 1557 2191 3434 4008 Size: 1557 634 1243 574 Fragments arranged by size: 1557 1243 634 574 NarI GG'CG_CC Cuts at: 0 4 39 599 1685 2648 2937 3065 4008 Size: 4 35 560 1086 963 289 128 943 Fragments arranged by size: 1086 963 943 560 289 128 35 4 NcoI C'CATG_G Cuts at: 0 22 350 3059 4008 Size: 22 328 2709 949 Fragments arranged by size: 2709 949 328 22 NgoMIV G'CCGG_C Cuts at: 0 1555 2189 3432 4008 Size: 1555 634 1243 576 Fragments arranged by size: 1555 1243 634 576 NspI r_CATG'y Cuts at: 0 3528 4008 Size: 3528 480 PasI CC'CwG_GG Cuts at: 0 313 985 1122 1174 1318 1789 1798 2028 Size: 313 672 137 52 144 471 9 230 Cuts at: 2028 2325 2848 2968 3034 3043 4008 Size: 297 523 120 66 9 965 Fragments arranged by size: 965 672 523 471 313 297 230 144 137 120 66 52 9 9 PfoI T'CCnGG_A Cuts at: 0 44 1476 1626 2211 2421 2607 2634 4008 Size: 44 1432 150 585 210 186 27 1374 Fragments arranged by size: 1432 1374 585 210 186 150 44 27 PmlI CAC'GTG Cuts at: 0 962 2839 4008 Size: 962 1877 1169 Fragments arranged by size: 1877 1169 962 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 3700 3732 4008 Size: 3700 32 276 Fragments arranged by size: 3700 276 32 PpuMI rG'GwC_Cy Cuts at: 0 147 681 1126 1168 1294 1345 1372 1453 Size: 147 534 445 42 126 51 27 81 Cuts at: 1453 1549 1585 1612 1897 2128 2179 2224 2269 Size: 96 36 27 285 231 51 45 45 Cuts at: 2269 2371 2527 2638 2860 2911 3624 4008 Size: 102 156 111 222 51 713 384 Fragments arranged by size: 713 534 445 384 285 231 222 156 147 126 111 102 96 81 51 51 51 45 45 42 36 27 27 PshAI GACnn'nnGTC Cuts at: 0 1292 4008 Size: 1292 2716 PspOMI G'GGCC_C Cuts at: 0 309 1044 1336 1363 1639 1792 1801 1855 Size: 309 735 292 27 276 153 9 54 Cuts at: 1855 2023 2328 2536 2628 2664 2943 2971 3027 Size: 168 305 208 92 36 279 28 56 Cuts at: 3027 3037 3046 4008 Size: 10 9 962 Fragments arranged by size: 962 735 309 305 292 279 276 208 168 153 92 56 54 36 28 27 10 9 9 PspXI vC'TCGA_Gb Cuts at: 0 1457 4008 Size: 1457 2551 PstI C_TGCA'G Cuts at: 0 137 1134 2223 3623 4008 Size: 137 997 1089 1400 385 Fragments arranged by size: 1400 1089 997 385 137 PvuII CAG'CTG Cuts at: 0 3207 3279 3704 4008 Size: 3207 72 425 304 Fragments arranged by size: 3207 425 304 72 SacI G_AGCT'C Cuts at: 0 930 3170 4008 Size: 930 2240 838 Fragments arranged by size: 2240 930 838 SacII CC_GC'GG Cuts at: 0 31 98 3304 3540 4008 Size: 31 67 3206 236 468 Fragments arranged by size: 3206 468 236 67 31 SanDI GG'GwC_CC Cuts at: 0 147 681 1585 1612 2269 2911 4008 Size: 147 534 904 27 657 642 1097 Fragments arranged by size: 1097 904 657 642 534 147 27 SapI GCTCTTCn'nnn_ Cuts at: 0 613 3802 4008 Size: 613 3189 206 Fragments arranged by size: 3189 613 206 SbfI CC_TGCA'GG Cuts at: 0 1134 2223 3623 4008 Size: 1134 1089 1400 385 Fragments arranged by size: 1400 1134 1089 385 SexAI A'CCwGG_T Cuts at: 0 423 1086 3491 4008 Size: 423 663 2405 517 Fragments arranged by size: 2405 663 517 423 SfcI C'TryA_G Cuts at: 0 133 1130 2219 3619 4008 Size: 133 997 1089 1400 389 Fragments arranged by size: 1400 1089 997 389 133 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1052 1061 1362 1800 2031 2108 2822 2831 Size: 1052 9 301 438 231 77 714 9 Cuts at: 2831 2891 2951 2960 3026 3045 4008 Size: 60 60 9 66 19 963 Fragments arranged by size: 1052 963 714 438 301 231 77 66 60 60 19 9 9 9 SfoI GGC'GCC Cuts at: 0 5 40 600 1686 2649 2938 3066 4008 Size: 5 35 560 1086 963 289 128 942 Fragments arranged by size: 1086 963 942 560 289 128 35 5 SmaI CCC'GGG Cuts at: 0 775 1097 1275 1593 1610 1986 2126 2267 Size: 775 322 178 318 17 376 140 141 Cuts at: 2267 2378 2442 2601 2781 3747 4008 Size: 111 64 159 180 966 261 Fragments arranged by size: 966 775 376 322 318 261 180 178 159 141 140 111 64 17 SmlI C'TyrA_G Cuts at: 0 1457 1918 3679 4008 Size: 1457 461 1761 329 Fragments arranged by size: 1761 1457 461 329 SphI G_CATG'C Cuts at: 0 3528 4008 Size: 3528 480 StuI AGG'CCT Cuts at: 0 1499 3904 4008 Size: 1499 2405 104 Fragments arranged by size: 2405 1499 104 StyI C'CwwG_G Cuts at: 0 22 141 350 851 1298 1350 2228 2273 Size: 22 119 209 501 447 52 878 45 Cuts at: 2273 2348 2490 2642 2864 3059 4008 Size: 75 142 152 222 195 949 Fragments arranged by size: 949 878 501 447 222 209 195 152 142 119 75 52 45 22 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 184 557 1663 2756 3865 4008 Size: 184 373 1106 1093 1109 143 Fragments arranged by size: 1109 1106 1093 373 184 143 TatI w'GTAC_w Cuts at: 0 3629 4008 Size: 3629 379 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 269 301 1253 1285 3397 3429 4008 Size: 269 32 952 32 2112 32 579 Fragments arranged by size: 2112 952 579 269 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 2086 4008 Size: 2086 1922 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 357 705 849 2235 2658 4008 Size: 357 348 144 1386 423 1350 Fragments arranged by size: 1386 1350 423 357 348 144 XhoI C'TCGA_G Cuts at: 0 1457 4008 Size: 1457 2551 XmaI C'CCGG_G Cuts at: 0 773 1095 1273 1591 1608 1984 2124 2265 Size: 773 322 178 318 17 376 140 141 Cuts at: 2265 2376 2440 2599 2779 3745 4008 Size: 111 64 159 180 966 263 Fragments arranged by size: 966 773 376 322 318 263 180 178 159 141 140 111 64 17 XmnI GAAnn'nnTTC Cuts at: 0 3190 3988 4008 Size: 3190 798 20 Fragments arranged by size: 3190 798 20 ZraI GAC'GTC Cuts at: 0 189 1542 3780 4008 Size: 189 1353 2238 228 Fragments arranged by size: 2238 1353 228 189 Enzymes that do cut: AarI AatII AccI Acc65I AcuI AfeI AflII AflIII AhdI AloI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI BcgI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstEII BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI FspI HaeII Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PfoI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI PvuII SacI SacII SanDI SapI SbfI SexAI SfcI SfiI SfoI SmaI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AclI AgeI AleI AlfI ApoI AscI AseI AsiSI AvrII BaeI BbvCI BciVI BclI BfrBI BglII BmtI BsaBI BsiWI BssHII BstAPI BstBI BstXI BstZ17I ClaI CspCI DraI DraIII EcoRI EcoRV FspAI HindIII HpaI MfeI MluI NdeI NheI NotI NruI NsiI PacI PciI PflMI PmeI PsiI PsrI PvuI RsrII SalI ScaI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XbaI