################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BbeI BciVI BfrI BlnI BsaBI BseAI BsiWI Bsp1407I BspEI BspHI BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I CpoI DraI DraIII Eam1105I EarI EcoNI EcoO65I EcoRV EcoT22I FseI FspI HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XhoI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 682 GACGTC GACGT^C AcsI 235 GAATTC [GA]^AATT[CT] AcyI 682 GACGTC G[GA]^CG[CT]C AflIII 253 ACATGT A^C[GA][CT]GT AlwNI 1024 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 407 CCTCAGG CC^T[ACGT]AGG ApaI 124 GGGCCC GGGCC^C ApoI 235 GAATTC [GA]^AATT[CT] BamHI 1280 GGATCC G^GATCC BbrPI 146 CACGTG CAC^GTG BbvCI 1010 CCTCAGC CCTCAGC(-5/-2) BcgI 174 CGAGGGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 263 TGATCA T^GATCA BglI 1783 GCCCAGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 682 GACGTC G[GA]^CG[CT]C BsaWI 1518 TCCGGT [AT]^CCGG[AT] BspDI 688 ATCGAT AT^CGAT BspLU11I 253 ACATGT A^CATGT BspMI 772 ACCTGC ACCTGC(4/8) BsrFI 988 ACCGGC [GA]^CCGG[CT] BstAPI 544 GCAAACGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 407 CCTCAGG CC^T[ACGT]AGG Cfr10I 988 ACCGGC [GA]^CCGG[CT] ClaI 688 ATCGAT AT^CGAT DrdI 1417 GACTTCCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 407 CCTCAGG CC^T[ACGT]AGG EcoRI 235 GAATTC G^AATTC FbaI 263 TGATCA T^GATCA Hin1I 682 GACGTC G[GA]^CG[CT]C HincII 1841 GTCAAC GT[CT]^[GA]AC HindII 1841 GTCAAC GT[CT]^[GA]AC MseI 1826 TTAA T^TAA NcoI 836 CCATGG C^CATGG PmaCI 146 CACGTG CAC^GTG PmlI 146 CACGTG CAC^GTG PshAI 162 GACGACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 1312 GAGCTC GAGCT^C SexAI 611 ACCTGGT A^CC[AT]GGT Tru9I 1826 TTAA T^TAA Tsp509I 236 AATT ^AATT XbaI 55 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccII 904 CGCG CG^CG 1846 CGCG AspHI 49 GTGCTC G[AT]GC[AT]^C 1312 GAGCTC BbsI 1458 GAAGAC GAAGAC(2/6) 982 GTCTTC BglII 443 AGATCT A^GATCT 532 AGATCT BpmI 83 CTCCAG CTGGAG(16/14) 1234 CTGGAG BpuAI 1458 GAAGAC GAAGAC(2/6) 982 GTCTTC BsaAI 146 CACGTG [CT]AC^GT[GA] 961 TACGTG BsiHKAI 49 GTGCTC G[AT]GC[AT]^C 1312 GAGCTC BsmI 281 GCATTC GAATGC(1/-1) 1562 GCATTC BsrBI 36 CCGCTC CCGCTC(-3/-3) 1276 GAGCGG BssSI 862 CACGAG CACGAG(-5/-1) 1688 CTCGTG BstUI 904 CGCG CG^CG 1846 CGCG BstXI 716 CCACCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1173 CCAGCTTGGTGG DsaI 434 CCGTGG C^C[GA][CT]GG 836 CCATGG EcoT14I 836 CCATGG C^C[AT][AT]GG 998 CCAAGG MslI 777 CACAGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 783 CATGGCGGTG MvnI 904 CGCG CG^CG 1846 CGCG NspI 253 ACATGT [GA]CATG^[CT] 462 GCATGT PleI 1640 GACTC GAGTC(4/5) 1091 GAGTC PvuII 731 CAGCTG CAG^CTG 1348 CAGCTG SmaI 44 CCCGGG CCC^GGG 560 CCCGGG StyI 836 CCATGG C^C[AT][AT]GG 998 CCAAGG TfiI 302 GAATC G^A[AT]TC 878 GAATC XmaCI 44 CCCGGG C^CCGGG 560 CCCGGG XmaI 44 CCCGGG C^CCGGG 560 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 93 enzymes) # ######################################### AciI AfaI AluI AlwI Aor51HI AvaI AvaII BanI BanII BbvI BcnI BfaI BlpI BmyI Bpu1102I BsaI BsaJI BseRI BsgI BsiEI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII EaeI EagI EclXI Eco47III Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI TaqI TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII