(Circular) (Six-base) MAPSORT of: pFN21AB8375.seq Check: 9536 from: 1 to: 6682 pFN21AB8375 6682 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2691 4492 278 Size: 53 83 186 936 1155 1801 2468 Fragments arranged by size: 2468 1801 1155 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4025 1937 Size: 2088 4594 Acc65I G'GTAC_C Cuts at: 4003 4003 Size: 6682 AclI AA'CG_TT Cuts at: 1563 4809 5182 1563 Size: 3246 373 3063 Fragments arranged by size: 3246 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2475 3056 3737 4745 6129 6397 1632 Size: 843 581 681 1008 1384 268 1917 Fragments arranged by size: 1917 1384 1008 843 681 581 268 AfeI AGC'GCT Cuts at: 2927 2956 3253 3653 2927 Size: 29 297 400 5956 Fragments arranged by size: 5956 400 297 29 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6485 AflIII A'CryG_T Cuts at: 1203 2258 5581 6558 1203 Size: 1055 3323 977 1327 Fragments arranged by size: 3323 1327 1055 977 AgeI A'CCGG_T Cuts at: 5513 5513 Size: 6682 AhdI GACnn_n'nnGTC Cuts at: 5411 5411 Size: 6682 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6682 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6682 AlwNI CAG_nnn'CTG Cuts at: 3034 4425 5997 3034 Size: 1391 1572 3719 Fragments arranged by size: 3719 1572 1391 ApaI G_GGCC'C Cuts at: 2133 2133 Size: 6682 ApaLI G'TGCA_C Cuts at: 4739 5895 4739 Size: 1156 5526 ApoI r'AATT_y Cuts at: 1493 2240 3991 4217 4369 6478 1493 Size: 747 1751 226 152 2109 1697 Fragments arranged by size: 2109 1751 1697 747 226 152 AseI AT'TA_AT Cuts at: 160 1032 5236 160 Size: 872 4204 1606 Fragments arranged by size: 4204 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6682 AvaI C'yCGr_G Cuts at: 1870 1943 2049 2565 3149 4007 1870 Size: 73 106 516 584 858 4545 Fragments arranged by size: 4545 858 584 516 106 73 BamHI G'GATC_C Cuts at: 3285 4012 4379 3285 Size: 727 367 5588 Fragments arranged by size: 5588 727 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2123 2524 2935 3839 4003 Size: 325 217 627 336 401 411 904 164 Cuts at: 4003 5458 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 904 627 411 401 336 325 217 164 BanII G_rGCy'C Cuts at: 1391 2068 2133 2535 3207 3321 3585 4001 1391 Size: 677 65 402 672 114 264 416 4072 Fragments arranged by size: 4072 677 672 416 402 264 114 65 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2980 3470 928 Size: 971 1081 490 4140 Fragments arranged by size: 4140 1081 971 490 BbvCI CC'TCA_GC Cuts at: 3016 3016 Size: 6682 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2168 2202 4873 4907 1903 Size: 34 50 34 147 34 2671 34 3678 Fragments arranged by size: 3678 2671 147 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4574 5784 4574 Size: 1210 5472 BclI T'GATC_A Cuts at: 1548 2268 1548 Size: 720 5962 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3794 5293 136 Size: 107 122 71 631 605 2122 1499 1525 Fragments arranged by size: 2122 1525 1499 631 605 122 107 71 BglII A'GATC_T Cuts at: 2448 2537 6677 2448 Size: 89 4140 2453 Fragments arranged by size: 4140 2453 89 BlpI GC'TnA_GC Cuts at: 2027 2945 3356 4099 2027 Size: 918 411 743 4610 Fragments arranged by size: 4610 918 743 411 Bme1580I G_kGCm'C Cuts at: 1790 2133 3700 3842 4743 5899 1790 Size: 343 1567 142 901 1156 2573 Fragments arranged by size: 2573 1567 1156 901 343 142 BmgBI CAC'GTC Cuts at: 1318 2608 2820 3328 1318 Size: 1290 212 508 4672 Fragments arranged by size: 4672 1290 508 212 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2199 3688 4413 Size: 416 53 83 399 24 782 1489 725 Cuts at: 4413 5371 442 Size: 958 1753 Fragments arranged by size: 1753 1489 958 782 725 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6682 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2073 3260 5342 1239 Size: 834 1187 2082 2579 Fragments arranged by size: 2579 2082 1187 834 Bpu10I CC'TnA_GC Cuts at: 3016 3299 3016 Size: 283 6399 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2834 3632 3791 4807 5672 5970 2834 Size: 798 159 1016 865 298 3546 Fragments arranged by size: 3546 1016 865 798 298 159 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2412 3436 3920 5345 882 Size: 617 913 1024 484 1425 2219 Fragments arranged by size: 2219 1425 1024 913 617 484 BsaAI yAC'GTr Cuts at: 493 1204 2153 2968 4473 493 Size: 711 949 815 1505 2702 Fragments arranged by size: 2702 1505 949 815 711 BsaBI GATnn'nnATC Cuts at: 6676 6676 Size: 6682 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2688 4489 4871 5506 Size: 53 83 186 936 1155 1801 382 635 Cuts at: 5506 275 Size: 1451 Fragments arranged by size: 1801 1451 1155 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3523 5115 5513 5787 5934 6345 3523 Size: 1592 398 274 147 411 3860 Fragments arranged by size: 3860 1592 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2543 2573 3372 3402 3614 Size: 30 165 30 1140 30 799 30 212 Cuts at: 3614 3644 1178 Size: 30 4216 Fragments arranged by size: 4216 1140 799 212 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2387 3329 3401 3749 1186 Size: 1201 942 72 348 4119 Fragments arranged by size: 4119 1201 942 348 72 BseYI C'CCAG_C Cuts at: 2716 3101 3507 3556 3626 3789 3965 5885 6381 Size: 385 406 49 70 163 176 1920 496 Cuts at: 6381 2716 Size: 3017 Fragments arranged by size: 3017 1920 496 406 385 176 163 70 49 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2255 2762 2764 2876 1163 Size: 573 519 507 2 112 4969 Fragments arranged by size: 4969 573 519 507 112 2 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2439 3109 3265 3404 3720 4132 Size: 275 186 438 670 156 139 316 412 Cuts at: 4132 4893 5921 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 761 670 438 412 316 275 186 156 139 BsiHKAI G_wGCw'C Cuts at: 2058 3321 4743 4828 5899 2058 Size: 1263 1422 85 1071 2841 Fragments arranged by size: 2841 1422 1263 1071 85 BsmI GAATG_Cn' Cuts at: 2286 3567 4193 4286 2286 Size: 1281 626 93 4682 Fragments arranged by size: 4682 1281 626 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2022 2159 2810 3083 3733 2022 Size: 137 651 273 650 4971 Fragments arranged by size: 4971 651 650 273 137 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2058 2068 2133 2535 3207 3321 3585 Size: 399 268 10 65 402 672 114 264 Cuts at: 3585 3700 3842 4001 4743 4828 5899 1391 Size: 115 142 159 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 672 402 399 268 264 159 142 115 114 85 65 10 BspEI T'CCGG_A Cuts at: 6345 6345 Size: 6682 BspHI T'CATG_A Cuts at: 4571 6301 4571 Size: 1730 4952 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2786 4037 844 Size: 1942 1251 3489 Fragments arranged by size: 3489 1942 1251 BsrBI CCG'CTC Cuts at: 1394 1823 2043 3283 4569 1394 Size: 429 220 1240 1286 3507 Fragments arranged by size: 3507 1286 1240 429 220 BsrDI GCAATG_nn' Cuts at: 65 1248 5177 5351 65 Size: 1183 3929 174 1396 Fragments arranged by size: 3929 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2993 5326 5513 1700 Size: 110 1183 2333 187 2869 Fragments arranged by size: 2869 2333 1183 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6682 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6682 BssSI C'ACGA_G Cuts at: 2867 3693 4742 5754 2867 Size: 826 1049 1012 3795 Fragments arranged by size: 3795 1049 1012 826 BstAPI GCAn_nnn'nTGC Cuts at: 794 2555 794 Size: 1761 4921 BstBI TT'CG_AA Cuts at: 5486 5486 Size: 6682 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6682 BstXI CCAn_nnnn'nTGG Cuts at: 2728 3185 2728 Size: 457 6225 BstYI r'GATC_y Cuts at: 1972 2448 2537 2575 3285 4012 4379 4764 4781 Size: 476 89 38 710 727 367 385 17 Cuts at: 4781 6233 6319 6331 6677 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 727 710 476 385 367 346 89 86 38 17 12 Bsu36I CC'TnA_GG Cuts at: 2413 2413 Size: 6682 BtgI C'CryG_G Cuts at: 513 1065 2439 2841 513 Size: 552 1374 402 4354 Fragments arranged by size: 4354 1374 552 402 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2126 487 Size: 683 56 900 5043 Fragments arranged by size: 5043 900 683 56 BtsI GCAGTG_nn' Cuts at: 3370 4198 4992 5012 3370 Size: 828 794 20 5040 Fragments arranged by size: 5040 828 794 20 ClaI AT'CG_AT Cuts at: 1554 2694 1554 Size: 1140 5542 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6647 DraI TTT'AAA Cuts at: 3986 3986 Size: 6682 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3428 5689 809 Size: 507 2112 2261 1802 Fragments arranged by size: 2261 2112 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2436 2995 3106 Size: 54 1425 50 275 18 606 559 111 Cuts at: 3106 3262 3701 3717 4129 5018 6647 8 Size: 156 439 16 412 889 1629 43 Fragments arranged by size: 1629 1425 889 606 559 439 412 275 156 111 54 50 43 18 16 EagI C'GGCC_G Cuts at: 1537 1812 2436 3106 3262 3717 4129 1537 Size: 275 624 670 156 455 412 4090 Fragments arranged by size: 4090 670 624 455 412 275 156 EarI CTCTTCn'nnn_ Cuts at: 1353 4612 1353 Size: 3259 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2035 5269 5643 5789 387 Size: 1064 138 446 3234 374 146 1280 Fragments arranged by size: 3234 1280 1064 446 374 146 138 EcoICRI GAG'CTC Cuts at: 3319 3319 Size: 6682 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2073 2475 3056 3260 3737 4745 5342 Size: 393 441 402 581 204 477 1008 597 Cuts at: 5342 6129 6397 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1008 787 597 581 477 441 402 393 268 204 EcoO109I rG'GnC_Cy Cuts at: 2130 2251 3060 3295 3683 2130 Size: 121 809 235 388 5129 Fragments arranged by size: 5129 809 388 235 121 EcoRI G'AATT_C Cuts at: 2240 3991 4369 2240 Size: 1751 378 4553 Fragments arranged by size: 4553 1751 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2052 2052 Size: 6682 FspI TGC'GCA Cuts at: 5188 5188 Size: 6682 HaeII r_GCGC'y Cuts at: 1678 2929 2958 3035 3255 3655 5829 1678 Size: 1251 29 77 220 400 2174 2531 Fragments arranged by size: 2531 2174 1251 400 220 77 29 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2069 2101 3527 3559 5323 Size: 32 135 32 497 32 1426 32 1764 Cuts at: 5323 5355 5397 5429 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1764 1426 497 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3848 4026 4272 669 Size: 988 281 1910 178 246 3079 Fragments arranged by size: 3079 1910 988 281 246 178 HindIII A'AGCT_T Cuts at: 748 748 Size: 6682 HpaI GTT'AAC Cuts at: 4272 4272 Size: 6682 KpnI G_GTAC'C Cuts at: 4007 4007 Size: 6682 MfeI C'AATT_G Cuts at: 4281 4281 Size: 6682 MluI A'CGCG_T Cuts at: 6558 6558 Size: 6682 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2655 3957 5796 5980 1305 Size: 1350 1302 1839 184 2007 Fragments arranged by size: 2007 1839 1350 1302 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4860 Fragments arranged by size: 4860 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2786 2792 4640 4999 5158 518 Size: 648 1620 6 1848 359 159 2042 Fragments arranged by size: 2042 1848 1620 648 359 159 6 MspA1I CmG'CkG Cuts at: 1606 2435 2738 2811 2941 3105 3355 3793 3965 Size: 829 303 73 130 164 250 438 172 Cuts at: 3965 4098 4775 5923 6168 1606 Size: 133 677 1148 245 2120 Fragments arranged by size: 2120 1148 829 677 438 303 250 245 172 164 133 130 73 NcoI C'CATG_G Cuts at: 513 1065 2841 513 Size: 552 1776 4354 Fragments arranged by size: 4354 1776 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6682 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6682 NotI GC'GGCC_GC Cuts at: 4129 4129 Size: 6682 NspI r_CATG'y Cuts at: 1132 1229 2262 2471 4040 5585 1132 Size: 97 1033 209 1569 1545 2229 Fragments arranged by size: 2229 1569 1545 1033 209 97 PasI CC'CwG_GG Cuts at: 1348 3891 1348 Size: 2543 4139 PciI A'CATG_T Cuts at: 2258 5581 2258 Size: 3323 3359 PflMI CCAn_nnn'nTGG Cuts at: 1108 2872 3049 3217 1108 Size: 1764 177 168 4573 Fragments arranged by size: 4573 1764 177 168 PfoI T'CCnGG_A Cuts at: 2160 2323 2727 3237 3321 3609 2160 Size: 163 404 510 84 288 5233 Fragments arranged by size: 5233 510 404 288 163 84 PmeI GTTT'AAAC Cuts at: 3986 3986 Size: 6682 PmlI CAC'GTG Cuts at: 2153 2153 Size: 6682 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4751 4783 6264 6296 4751 Size: 32 1481 32 5137 Fragments arranged by size: 5137 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2251 3295 3683 2251 Size: 1044 388 5250 Fragments arranged by size: 5250 1044 388 PshAI GACnn'nnGTC Cuts at: 1613 2171 1613 Size: 558 6124 PsiI TTA'TAA Cuts at: 2226 4252 2226 Size: 2026 4656 PspOMI G'GGCC_C Cuts at: 2129 2129 Size: 6682 PstI C_TGCA'G Cuts at: 830 3127 3396 3594 4034 830 Size: 2297 269 198 440 3478 Fragments arranged by size: 3478 2297 440 269 198 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6682 PvuII CAG'CTG Cuts at: 2738 3355 2738 Size: 617 6065 SacI G_AGCT'C Cuts at: 3321 3321 Size: 6682 SalI G'TCGA_C Cuts at: 1936 4024 1936 Size: 2088 4594 SanDI GG'GwC_CC Cuts at: 3683 3683 Size: 6682 SbfI CC_TGCA'GG Cuts at: 4034 4034 Size: 6682 ScaI AGT'ACT Cuts at: 4930 6642 4930 Size: 1712 4970 SexAI A'CCwGG_T Cuts at: 2616 2616 Size: 6682 SfcI C'TryA_G Cuts at: 826 1045 2824 3123 3392 3590 4030 5165 5846 Size: 219 1779 299 269 198 440 1135 681 Cuts at: 5846 6037 826 Size: 191 1471 Fragments arranged by size: 1779 1471 1135 681 440 299 269 219 198 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6682 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6682 SmaI CCC'GGG Cuts at: 1872 2051 2567 4009 1872 Size: 179 516 1442 4545 Fragments arranged by size: 4545 1442 516 179 SmlI C'TyrA_G Cuts at: 820 1017 1943 2849 3647 3806 4786 5687 5949 Size: 197 926 906 798 159 980 901 262 Cuts at: 5949 820 Size: 1553 Fragments arranged by size: 1553 980 926 906 901 798 262 197 159 SnaBI TAC'GTA Cuts at: 493 4473 493 Size: 3980 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6682 SphI G_CATG'C Cuts at: 1132 4040 1132 Size: 2908 3774 SspI AAT'ATT Cuts at: 5 52 4606 5 Size: 47 4554 2081 Fragments arranged by size: 4554 2081 47 StuI AGG'CCT Cuts at: 1517 2463 2481 2496 3014 1517 Size: 946 18 15 518 5185 Fragments arranged by size: 5185 946 518 18 15 StyI C'CwwG_G Cuts at: 513 1065 2841 3003 4121 513 Size: 552 1776 162 1118 3074 Fragments arranged by size: 3074 1776 1118 552 162 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3904 4709 4726 4879 5064 5509 325 Size: 1255 2324 805 17 153 185 445 1498 Fragments arranged by size: 2324 1498 1255 805 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3336 4928 6640 Size: 274 80 33 51 1443 1359 1592 1712 Cuts at: 6640 96 Size: 138 Fragments arranged by size: 1712 1592 1443 1359 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2142 2174 3372 3404 6642 6674 2142 Size: 32 1198 32 3238 32 2150 Fragments arranged by size: 3238 2150 1198 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6682 XbaI T'CTAG_A Cuts at: 2060 4018 2060 Size: 1958 4724 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2569 3214 3944 2569 Size: 645 730 5307 Fragments arranged by size: 5307 730 645 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6682 XmaI C'CCGG_G Cuts at: 1870 2049 2565 4007 1870 Size: 179 516 1442 4545 Fragments arranged by size: 4545 1442 516 179 XmnI GAAnn'nnTTC Cuts at: 4811 4811 Size: 6682 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2689 4490 276 Size: 53 83 186 936 1155 1801 2468 Fragments arranged by size: 2468 1801 1155 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI