(Linear) (Six-base) MAPSORT of: KIBB8375.seq Check: 5830 from: 1 to: 1977 KIBB8375.orf With 182 enzymes: SgfI * July 15, 2010 18:31 .. AatII G_ACGT'C Cuts at: 0 686 1977 Size: 686 1291 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 470 1051 1732 1977 Size: 470 581 681 245 Fragments arranged by size: 681 581 470 245 AfeI AGC'GCT Cuts at: 0 922 951 1248 1648 1977 Size: 922 29 297 400 329 Fragments arranged by size: 922 400 329 297 29 AflIII A'CryG_T Cuts at: 0 253 1977 Size: 253 1724 AlwNI CAG_nnn'CTG Cuts at: 0 1029 1977 Size: 1029 948 ApaI G_GGCC'C Cuts at: 0 128 1977 Size: 128 1849 ApoI r'AATT_y Cuts at: 0 235 1977 Size: 235 1742 AvaI C'yCGr_G Cuts at: 0 44 560 1144 1977 Size: 44 516 584 833 Fragments arranged by size: 833 584 516 44 BamHI G'GATC_C Cuts at: 0 1280 1977 Size: 1280 697 BanI G'GyrC_C Cuts at: 0 118 519 930 1834 1977 Size: 118 401 411 904 143 Fragments arranged by size: 904 411 401 143 118 BanII G_rGCy'C Cuts at: 0 63 128 530 1202 1316 1580 1977 Size: 63 65 402 672 114 264 397 Fragments arranged by size: 672 402 397 264 114 65 63 BbsI GAAGACnn'nnnn_ Cuts at: 0 975 1465 1977 Size: 975 490 512 Fragments arranged by size: 975 512 490 BbvCI CC'TCA_GC Cuts at: 0 1011 1977 Size: 1011 966 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 163 197 1977 Size: 163 34 1780 Fragments arranged by size: 1780 163 34 BclI T'GATC_A Cuts at: 0 263 1977 Size: 263 1714 BglI GCCn_nnn'nGGC Cuts at: 0 1789 1977 Size: 1789 188 BglII A'GATC_T Cuts at: 0 443 532 1977 Size: 443 89 1445 Fragments arranged by size: 1445 443 89 BlpI GC'TnA_GC Cuts at: 0 22 940 1351 1977 Size: 22 918 411 626 Fragments arranged by size: 918 626 411 22 Bme1580I G_kGCm'C Cuts at: 0 128 1695 1837 1977 Size: 128 1567 142 140 Fragments arranged by size: 1567 142 140 128 BmgBI CAC'GTC Cuts at: 0 603 815 1323 1977 Size: 603 212 508 654 Fragments arranged by size: 654 603 508 212 BmrI ACTGGGnnnn_n' Cuts at: 0 194 1683 1977 Size: 194 1489 294 Fragments arranged by size: 1489 294 194 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 68 1255 1977 Size: 68 1187 722 Fragments arranged by size: 1187 722 68 Bpu10I CC'TnA_GC Cuts at: 0 1011 1294 1977 Size: 1011 283 683 Fragments arranged by size: 1011 683 283 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 829 1627 1786 1977 Size: 829 798 159 191 Fragments arranged by size: 829 798 191 159 BsaI GGTCTCn'nnnn_ Cuts at: 0 407 1431 1915 1977 Size: 407 1024 484 62 Fragments arranged by size: 1024 484 407 62 BsaAI yAC'GTr Cuts at: 0 148 963 1977 Size: 148 815 1014 Fragments arranged by size: 1014 815 148 BsaHI Gr'CG_yC Cuts at: 0 683 1977 Size: 683 1294 BsaWI w'CCGG_w Cuts at: 0 1518 1977 Size: 1518 459 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 538 568 1367 1397 1609 1639 1977 Size: 538 30 799 30 212 30 338 Fragments arranged by size: 799 538 338 212 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 382 1324 1396 1744 1977 Size: 382 942 72 348 233 Fragments arranged by size: 942 382 348 233 72 BseYI C'CCAG_C Cuts at: 0 711 1096 1502 1551 1621 1784 1960 1977 Size: 711 385 406 49 70 163 176 17 Fragments arranged by size: 711 406 385 176 163 70 49 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 250 757 759 871 1977 Size: 250 507 2 112 1106 Fragments arranged by size: 1106 507 250 112 2 BsiEI CG_ry'CG Cuts at: 0 434 1104 1260 1399 1715 1977 Size: 434 670 156 139 316 262 Fragments arranged by size: 670 434 316 262 156 139 BsiHKAI G_wGCw'C Cuts at: 0 53 1316 1977 Size: 53 1263 661 Fragments arranged by size: 1263 661 53 BsmI GAATG_Cn' Cuts at: 0 281 1562 1977 Size: 281 1281 415 Fragments arranged by size: 1281 415 281 BsmBI CGTCTCn'nnnn_ Cuts at: 0 17 154 805 1078 1728 1977 Size: 17 137 651 273 650 249 Fragments arranged by size: 651 650 273 249 137 17 Bsp1286I G_dGCh'C Cuts at: 0 53 63 128 530 1202 1316 1580 1695 Size: 53 10 65 402 672 114 264 115 Cuts at: 1695 1837 1977 Size: 142 140 Fragments arranged by size: 672 402 264 142 140 115 114 65 53 10 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 781 1977 Size: 781 1196 BsrBI CCG'CTC Cuts at: 0 38 1278 1977 Size: 38 1240 699 Fragments arranged by size: 1240 699 38 BsrFI r'CCGG_y Cuts at: 0 988 1977 Size: 988 989 BssSI C'ACGA_G Cuts at: 0 862 1688 1977 Size: 862 826 289 Fragments arranged by size: 862 826 289 BstAPI GCAn_nnn'nTGC Cuts at: 0 550 1977 Size: 550 1427 BstXI CCAn_nnnn'nTGG Cuts at: 0 723 1180 1977 Size: 723 457 797 Fragments arranged by size: 797 723 457 BstYI r'GATC_y Cuts at: 0 443 532 570 1280 1977 Size: 443 89 38 710 697 Fragments arranged by size: 710 697 443 89 38 Bsu36I CC'TnA_GG Cuts at: 0 408 1977 Size: 408 1569 BtgI C'CryG_G Cuts at: 0 434 836 1977 Size: 434 402 1141 Fragments arranged by size: 1141 434 402 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 121 1977 Size: 121 1856 BtsI GCAGTG_nn' Cuts at: 0 1365 1977 Size: 1365 612 ClaI AT'CG_AT Cuts at: 0 689 1977 Size: 689 1288 DrdI GACnn_nn'nnGTC Cuts at: 0 1423 1977 Size: 1423 554 EaeI y'GGCC_r Cuts at: 0 431 990 1101 1257 1696 1712 1977 Size: 431 559 111 156 439 16 265 Fragments arranged by size: 559 439 431 265 156 111 16 EagI C'GGCC_G Cuts at: 0 431 1101 1257 1712 1977 Size: 431 670 156 455 265 Fragments arranged by size: 670 455 431 265 156 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 30 1977 Size: 30 1947 EcoICRI GAG'CTC Cuts at: 0 1314 1977 Size: 1314 663 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 68 470 1051 1255 1732 1977 Size: 68 402 581 204 477 245 Fragments arranged by size: 581 477 402 245 204 68 EcoO109I rG'GnC_Cy Cuts at: 0 125 246 1055 1290 1678 1977 Size: 125 121 809 235 388 299 Fragments arranged by size: 809 388 299 235 125 121 EcoRI G'AATT_C Cuts at: 0 235 1977 Size: 235 1742 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 47 1977 Size: 47 1930 HaeII r_GCGC'y Cuts at: 0 924 953 1030 1250 1650 1977 Size: 924 29 77 220 400 327 Fragments arranged by size: 924 400 327 220 77 29 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 64 96 1522 1554 1977 Size: 64 32 1426 32 423 Fragments arranged by size: 1426 423 64 32 32 HincII GTy'rAC Cuts at: 0 1843 1977 Size: 1843 134 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 650 1952 1977 Size: 650 1302 25 Fragments arranged by size: 1302 650 25 MslI CAynn'nnrTG Cuts at: 0 781 787 1977 Size: 781 6 1190 Fragments arranged by size: 1190 781 6 MspA1I CmG'CkG Cuts at: 0 430 733 806 936 1100 1350 1788 1960 Size: 430 303 73 130 164 250 438 172 Cuts at: 1960 1977 Size: 17 Fragments arranged by size: 438 430 303 250 172 164 130 73 17 NcoI C'CATG_G Cuts at: 0 836 1977 Size: 836 1141 NspI r_CATG'y Cuts at: 0 257 466 1977 Size: 257 209 1511 Fragments arranged by size: 1511 257 209 PasI CC'CwG_GG Cuts at: 0 1886 1977 Size: 1886 91 PciI A'CATG_T Cuts at: 0 253 1977 Size: 253 1724 PflMI CCAn_nnn'nTGG Cuts at: 0 867 1044 1212 1977 Size: 867 177 168 765 Fragments arranged by size: 867 765 177 168 PfoI T'CCnGG_A Cuts at: 0 155 318 722 1232 1316 1604 1977 Size: 155 163 404 510 84 288 373 Fragments arranged by size: 510 404 373 288 163 155 84 PmlI CAC'GTG Cuts at: 0 148 1977 Size: 148 1829 PpuMI rG'GwC_Cy Cuts at: 0 246 1290 1678 1977 Size: 246 1044 388 299 Fragments arranged by size: 1044 388 299 246 PshAI GACnn'nnGTC Cuts at: 0 166 1977 Size: 166 1811 PsiI TTA'TAA Cuts at: 0 221 1977 Size: 221 1756 PspOMI G'GGCC_C Cuts at: 0 124 1977 Size: 124 1853 PstI C_TGCA'G Cuts at: 0 1122 1391 1589 1977 Size: 1122 269 198 388 Fragments arranged by size: 1122 388 269 198 PvuII CAG'CTG Cuts at: 0 733 1350 1977 Size: 733 617 627 Fragments arranged by size: 733 627 617 SacI G_AGCT'C Cuts at: 0 1316 1977 Size: 1316 661 SanDI GG'GwC_CC Cuts at: 0 1678 1977 Size: 1678 299 SexAI A'CCwGG_T Cuts at: 0 611 1977 Size: 611 1366 SfcI C'TryA_G Cuts at: 0 819 1118 1387 1585 1977 Size: 819 299 269 198 392 Fragments arranged by size: 819 392 299 269 198 SmaI CCC'GGG Cuts at: 0 46 562 1977 Size: 46 516 1415 Fragments arranged by size: 1415 516 46 SmlI C'TyrA_G Cuts at: 0 844 1642 1801 1977 Size: 844 798 159 176 Fragments arranged by size: 844 798 176 159 StuI AGG'CCT Cuts at: 0 458 476 491 1009 1977 Size: 458 18 15 518 968 Fragments arranged by size: 968 518 458 18 15 StyI C'CwwG_G Cuts at: 0 836 998 1977 Size: 836 162 979 Fragments arranged by size: 979 836 162 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1899 1977 Size: 1899 78 TatI w'GTAC_w Cuts at: 0 1331 1977 Size: 1331 646 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 137 169 1367 1399 1977 Size: 137 32 1198 32 578 Fragments arranged by size: 1198 578 137 32 32 XbaI T'CTAG_A Cuts at: 0 55 1977 Size: 55 1922 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 564 1209 1939 1977 Size: 564 645 730 38 Fragments arranged by size: 730 645 564 38 XmaI C'CCGG_G Cuts at: 0 44 560 1977 Size: 44 516 1417 Fragments arranged by size: 1417 516 44 ZraI GAC'GTC Cuts at: 0 684 1977 Size: 684 1293 Enzymes that do cut: AatII AcuI AfeI AflIII AlwNI ApaI ApoI AvaI BamHI BanI BanII BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DrdI EaeI EagI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII MmeI MslI MspA1I NcoI NspI PasI PciI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PstI PvuII SacI SanDI SexAI SfcI SmaI SmlI StuI StyI TaqII TatI TstI XbaI XcmI XmaI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AflII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BciVI BfrBI BmtI BplI BsaBI BsiWI BspEI BspHI BsrDI BsrGI BssHII BstBI BstEII BstZ17I CspCI DraI DraIII EarI EcoNI EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PpiI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XhoI XmnI