################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## Acc65I AccII AccIII AciI AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BanI BbeI BbrPI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeII HgaI HhaI HinP1I HpaI KasI KpnI KspI MamI MluI MroI MslI MvnI NaeI NarI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SapI Sau96I SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 776 GACGTC GACGT^C AccI 773 GTCGAC GT^[AC][GT]AC AcyI 776 GACGTC G[GA]^CG[CT]C BalI 138 TGGCCA TGG^CCA BbsI 251 GAAGAC GAAGAC(2/6) BbvCI 735 GCTGAGG CCTCAGC(-5/-2) BcnI 679 CCCGG CC^[GC]GG BpmI 805 CTGGAG CTGGAG(16/14) BpuAI 251 GAAGAC GAAGAC(2/6) BsaHI 776 GACGTC G[GA]^CG[CT]C BseRI 503 CTCCTC GAGGAG(10/8) BsgI 124 GTGCAG GTGCAG(16/14) BsmAI 184 GAGAC GTCTC(1/5) Bsp1407I 525 TGTACA T^GTACA BsrDI 408 CATTGC GCAATG(2/0) BsrGI 525 TGTACA T^GTACA BstAPI 172 GCAGCAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 72 CCAGCATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 509 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 138 TGGCCA [CT]^GGCC[GA] EarI 319 GAAGAG CTCTTC(1/4) HaeIII 139 GGCC GG^CC HapII 680 CCGG C^CGG Hin1I 776 GACGTC G[GA]^CG[CT]C HincII 773 GTCGAC GT[CT]^[GA]AC HindII 773 GTCGAC GT[CT]^[GA]AC HindIII 844 AAGCTT A^AGCTT HpaII 680 CCGG C^CGG Ksp632I 319 GAAGAG CTCTTC(1/4) MaeIII 608 GTGAC ^GT[ACGT]AC MluNI 138 TGGCCA TGG^CCA MscI 138 TGGCCA TGG^CCA MspI 680 CCGG C^CGG NciI 679 CCCGG CC^[GC]GG NspI 611 ACATGC [GA]CATG^[CT] PflMI 699 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 129 GACTC GAGTC(4/5) SalI 773 GTCGAC G^TCGAC SexAI 510 ACCAGGT A^CC[AT]GGT SfaNI 673 GCATC GCATC(5/9) SspBI 525 TGTACA T^GTACA Tsp45I 608 GTGAC ^GT[GC]AC Van91I 699 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcsI 144 AAATTC [GA]^AATT[CT] 492 AAATTC AlwI 627 GATCC GGATC(4/5) 451 GGATC ApoI 144 AAATTC [GA]^AATT[CT] 492 AAATTC AspHI 500 GAGCTC G[AT]GC[AT]^C 632 GAGCTC BanII 500 GAGCTC G[GA]GC[CT]^C 632 GAGCTC BmyI 500 GAGCTC G[AGT]GC[ACT]^C 632 GAGCTC BsiHKAI 500 GAGCTC G[AT]GC[AT]^C 632 GAGCTC Bsp1286I 500 GAGCTC G[AGT]GC[ACT]^C 632 GAGCTC BsrI 136 ACTGG ACTGG(1/-1) 604 ACTGG BstYI 451 GGATCT [GA]^GATC[CT] 626 AGATCC Cac8I 474 GCAGGC GC[ACGT]^[ACGT]GC 705 GCTGGC DsaI 53 CCATGG C^C[GA][CT]GG 362 CCATGG EcoNI 257 CCTCTTATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 505 CCTCCACCAGG HphI 607 GGTGA GGTGA(8/7) 663 GGTGA MaeII 231 ACGT A^CGT 777 ACGT MfeI 176 CAATTG C^AATTG 521 CAATTG MflI 451 GGATCT [GA]^GATC[CT] 626 AGATCC MseI 29 TTAA T^TAA 481 TTAA MspA1I 545 CAGCTG C[AC]G^C[GT]G 703 CAGCTG MunI 176 CAATTG C^AATTG 521 CAATTG NcoI 53 CCATGG C^CATGG 362 CCATGG PstI 197 CTGCAG CTGCA^G 472 CTGCAG PvuII 545 CAGCTG CAG^CTG 703 CAGCTG SacI 500 GAGCTC GAGCT^C 632 GAGCTC TaqI 774 TCGA T^CGA 780 TCGA Tru9I 29 TTAA T^TAA 481 TTAA TspRI 603 CACTG CA[GC]TG[ACGT][ACGT]^ 821 CAGTG TthHB8I 774 TCGA T^CGA 780 TCGA XhoII 451 GGATCT [GA]^GATC[CT] 626 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AfaI AluI AlwNI BbvI BfaI BsaJI BsiYI BslI BsmFI BssKI BstNI DdeI DpnII EcoRII EcoT14I Fnu4HI HinfI ItaI MaeI MboI MboII MnlI MvaI MwoI NdeII NlaIII RsaI Sau3AI ScrFI SfcI StyI TfiI TseI Tsp509I