(Circular) (Six-base) MAPSORT of: pFN21AB6113.seq Check: 5729 from: 1 to: 6565 pFN21AB6113 6565 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4375 278 Size: 53 83 186 936 2839 2468 Fragments arranged by size: 2839 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3765 3908 1937 Size: 1828 143 4594 Fragments arranged by size: 4594 1828 143 Acc65I G'GTAC_C Cuts at: 3886 3886 Size: 6565 AclI AA'CG_TT Cuts at: 1563 4692 5065 1563 Size: 3129 373 3063 Fragments arranged by size: 3129 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2177 2508 2538 3246 4628 6012 6280 1632 Size: 545 331 30 708 1382 1384 268 1917 Fragments arranged by size: 1917 1384 1382 708 545 331 268 30 AfeI AGC'GCT Cuts at: 2306 2306 Size: 6565 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6368 AflIII A'CryG_T Cuts at: 1203 3760 5464 6441 1203 Size: 2557 1704 977 1327 Fragments arranged by size: 2557 1704 1327 977 AgeI A'CCGG_T Cuts at: 5396 5396 Size: 6565 AhdI GACnn_n'nnGTC Cuts at: 5294 5294 Size: 6565 AleI CACnn'nnGTG Cuts at: 1166 3098 1166 Size: 1932 4633 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6565 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2386 2418 2386 Size: 32 6533 AlwNI CAG_nnn'CTG Cuts at: 4308 5880 4308 Size: 1572 4993 ApaLI G'TGCA_C Cuts at: 4622 5778 4622 Size: 1156 5409 ApoI r'AATT_y Cuts at: 1493 3560 3874 4100 4252 6361 1493 Size: 2067 314 226 152 2109 1697 Fragments arranged by size: 2109 2067 1697 314 226 152 AseI AT'TA_AT Cuts at: 160 1032 5119 160 Size: 872 4087 1606 Fragments arranged by size: 4087 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6565 AvaI C'yCGr_G Cuts at: 1870 1943 3890 1870 Size: 73 1947 4545 Fragments arranged by size: 4545 1947 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2993 3026 3703 3736 2993 Size: 33 677 33 5822 Fragments arranged by size: 5822 677 33 33 BamHI G'GATC_C Cuts at: 3895 4262 3895 Size: 367 6198 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2353 3554 3886 5341 618 Size: 325 217 627 566 1201 332 1455 1842 Fragments arranged by size: 1842 1455 1201 627 566 332 325 217 BanII G_rGCy'C Cuts at: 1391 2175 3093 3884 1391 Size: 784 918 791 4072 Fragments arranged by size: 4072 918 791 784 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2496 3717 928 Size: 971 597 1221 3776 Fragments arranged by size: 3776 1221 971 597 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4756 4790 1903 Size: 34 2819 34 3678 Fragments arranged by size: 3678 2819 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3021 4457 5667 3021 Size: 1436 1210 3919 Fragments arranged by size: 3919 1436 1210 BclI T'GATC_A Cuts at: 1548 3532 1548 Size: 1984 4581 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2117 5176 136 Size: 107 122 71 631 605 445 3059 1525 Fragments arranged by size: 3059 1525 631 605 445 122 107 71 BglII A'GATC_T Cuts at: 6560 6560 Size: 6565 BlpI GC'TnA_GC Cuts at: 3982 3982 Size: 6565 Bme1580I G_kGCm'C Cuts at: 1790 2437 4626 5782 1790 Size: 647 2189 1156 2573 Fragments arranged by size: 2573 2189 1156 647 BmgBI CAC'GTC Cuts at: 1318 3763 1318 Size: 2445 4120 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3035 4296 5254 Size: 416 53 83 399 24 1618 1261 958 Cuts at: 5254 442 Size: 1753 Fragments arranged by size: 1753 1618 1261 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6565 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3610 3610 Size: 6565 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2268 2432 3302 3805 5225 1239 Size: 1029 164 870 503 1420 2579 Fragments arranged by size: 2579 1420 1029 870 503 164 Bpu10I CC'TnA_GC Cuts at: 2910 2910 Size: 6565 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4690 5555 5853 4690 Size: 865 298 5402 Fragments arranged by size: 5402 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3368 3790 5228 882 Size: 617 1869 422 1438 2219 Fragments arranged by size: 2219 1869 1438 617 422 BsaAI yAC'GTr Cuts at: 493 1204 4356 493 Size: 711 3152 2702 Fragments arranged by size: 3152 2702 711 BsaBI GATnn'nnATC Cuts at: 6559 6559 Size: 6565 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4372 4754 5389 275 Size: 53 83 186 936 2839 382 635 1451 Fragments arranged by size: 2839 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3006 3722 4998 5396 5670 5817 6228 3006 Size: 716 1276 398 274 147 411 3343 Fragments arranged by size: 3343 1276 716 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2386 2416 2979 3009 1178 Size: 30 165 30 983 30 563 30 4734 Fragments arranged by size: 4734 983 563 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3000 3233 1186 Size: 1814 233 4518 Fragments arranged by size: 4518 1814 233 BseYI C'CCAG_C Cuts at: 2307 3351 3382 5768 6264 2307 Size: 1044 31 2386 496 2608 Fragments arranged by size: 2608 2386 1044 496 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3281 1163 Size: 573 1545 4447 Fragments arranged by size: 4447 1545 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4015 4776 5804 1540 Size: 275 186 2014 761 1028 2301 Fragments arranged by size: 2301 2014 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2175 3093 3324 4626 4711 5782 2175 Size: 918 231 1302 85 1071 2958 Fragments arranged by size: 2958 1302 1071 918 231 85 BsmI GAATG_Cn' Cuts at: 2461 4076 4169 2461 Size: 1615 93 4857 Fragments arranged by size: 4857 1615 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2175 2437 3093 3324 3884 4626 4711 Size: 399 385 262 656 231 560 742 85 Cuts at: 4711 5782 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 656 560 399 385 262 231 85 BspEI T'CCGG_A Cuts at: 6228 6228 Size: 6565 BspHI T'CATG_A Cuts at: 4454 6184 4454 Size: 1730 4835 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3920 844 Size: 3076 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4452 1394 Size: 429 2629 3507 Fragments arranged by size: 3507 2629 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2532 5060 5234 65 Size: 1183 1284 2528 174 1396 Fragments arranged by size: 2528 1396 1284 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2298 5209 5396 1700 Size: 110 488 2911 187 2869 Fragments arranged by size: 2911 2869 488 187 110 BsrGI T'GTAC_A Cuts at: 96 3735 96 Size: 3639 2926 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6565 BssSI C'ACGA_G Cuts at: 4625 5637 4625 Size: 1012 5553 BstAPI GCAn_nnn'nTGC Cuts at: 794 2223 794 Size: 1429 5136 BstBI TT'CG_AA Cuts at: 5369 5369 Size: 6565 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6565 BstXI CCAn_nnnn'nTGG Cuts at: 2981 3301 2981 Size: 320 6245 BstYI r'GATC_y Cuts at: 1972 3895 4262 4647 4664 6116 6202 6214 6560 Size: 1923 367 385 17 1452 86 12 346 Cuts at: 6560 1972 Size: 1977 Fragments arranged by size: 1977 1923 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2565 2565 Size: 6565 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6013 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5826 Fragments arranged by size: 5826 683 56 BtsI GCAGTG_nn' Cuts at: 2576 3121 3781 4081 4875 4895 2576 Size: 545 660 300 794 20 4246 Fragments arranged by size: 4246 794 660 545 300 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6565 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6530 DraI TTT'AAA Cuts at: 2083 3869 2083 Size: 1786 4779 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3831 5572 809 Size: 507 2515 1741 1802 Fragments arranged by size: 2515 1802 1741 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3809 4012 4901 Size: 54 1425 50 275 18 1979 203 889 Cuts at: 4901 6530 8 Size: 1629 43 Fragments arranged by size: 1979 1629 1425 889 275 203 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4012 1537 Size: 275 2200 4090 Fragments arranged by size: 4090 2200 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2478 2586 2961 3198 3420 4495 1353 Size: 1125 108 375 237 222 1075 3423 Fragments arranged by size: 3423 1125 1075 375 237 222 108 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5152 5526 5672 387 Size: 1064 138 3563 374 146 1280 Fragments arranged by size: 3563 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2173 3091 2173 Size: 918 5647 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2177 2268 2432 2508 2538 3246 3302 Size: 393 545 91 164 76 30 708 56 Cuts at: 3302 3805 4628 5225 6012 6280 1239 Size: 503 823 597 787 268 1524 Fragments arranged by size: 1524 823 787 708 597 545 503 393 268 164 91 76 56 30 EcoNI CCTnn'n_nnAGG Cuts at: 3284 3425 3284 Size: 141 6424 EcoO109I rG'GnC_Cy Cuts at: 3289 3289 Size: 6565 EcoRI G'AATT_C Cuts at: 3874 4252 3874 Size: 378 6187 FspI TGC'GCA Cuts at: 5071 5071 Size: 6565 HaeII r_GCGC'y Cuts at: 1678 2308 5712 1678 Size: 630 3404 2531 Fragments arranged by size: 3404 2531 630 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3614 3646 3763 3795 5206 Size: 32 135 32 2042 32 117 32 1411 Cuts at: 5206 5238 5280 5312 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2042 1411 135 117 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2103 3836 3909 4155 669 Size: 988 281 165 1733 73 246 3079 Fragments arranged by size: 3079 1733 988 281 246 165 73 HindIII A'AGCT_T Cuts at: 748 2417 748 Size: 1669 4896 HpaI GTT'AAC Cuts at: 2103 4155 2103 Size: 2052 4513 KpnI G_GTAC'C Cuts at: 3890 3890 Size: 6565 MfeI C'AATT_G Cuts at: 4164 4164 Size: 6565 MluI A'CGCG_T Cuts at: 6441 6441 Size: 6565 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2471 5679 5863 1305 Size: 1166 3208 184 2007 Fragments arranged by size: 3208 2007 1166 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4743 Fragments arranged by size: 4743 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3098 3494 4523 4882 5041 518 Size: 648 1932 396 1029 359 159 2042 Fragments arranged by size: 2042 1932 1029 648 396 359 159 MspA1I CmG'CkG Cuts at: 1606 2410 3265 3355 3981 4658 5806 6051 1606 Size: 804 855 90 626 677 1148 245 2120 Fragments arranged by size: 2120 1148 855 804 677 626 245 90 NaeI GCC'GGC Cuts at: 2300 2300 Size: 6565 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6013 NdeI CA'TA_TG Cuts at: 387 387 Size: 6565 NgoMIV G'CCGG_C Cuts at: 2298 2298 Size: 6565 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6565 NotI GC'GGCC_GC Cuts at: 4012 4012 Size: 6565 NspI r_CATG'y Cuts at: 1132 1229 3391 3435 3923 5468 1132 Size: 97 2162 44 488 1545 2229 Fragments arranged by size: 2229 2162 1545 488 97 44 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6565 PciI A'CATG_T Cuts at: 5464 5464 Size: 6565 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6565 PfoI T'CCnGG_A Cuts at: 2022 2637 2804 2022 Size: 615 167 5783 Fragments arranged by size: 5783 615 167 PmeI GTTT'AAAC Cuts at: 3869 3869 Size: 6565 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2386 2418 4634 4666 6147 6179 2386 Size: 32 2216 32 1481 32 2772 Fragments arranged by size: 2772 2216 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6565 PsiI TTA'TAA Cuts at: 4135 4135 Size: 6565 PstI C_TGCA'G Cuts at: 830 2815 3373 3917 830 Size: 1985 558 544 3478 Fragments arranged by size: 3478 1985 558 544 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6565 PvuII CAG'CTG Cuts at: 2410 3265 2410 Size: 855 5710 SacI G_AGCT'C Cuts at: 2175 3093 2175 Size: 918 5647 SalI G'TCGA_C Cuts at: 1936 3907 1936 Size: 1971 4594 SbfI CC_TGCA'GG Cuts at: 3917 3917 Size: 6565 ScaI AGT'ACT Cuts at: 2597 4813 6525 2597 Size: 2216 1712 2637 Fragments arranged by size: 2637 2216 1712 SfcI C'TryA_G Cuts at: 826 1045 2811 3369 3607 3913 5048 5729 5920 Size: 219 1766 558 238 306 1135 681 191 Cuts at: 5920 826 Size: 1471 Fragments arranged by size: 1766 1471 1135 681 558 306 238 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6565 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6565 SmaI CCC'GGG Cuts at: 1872 3892 1872 Size: 2020 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4669 5570 5832 820 Size: 197 926 2726 901 262 1553 Fragments arranged by size: 2726 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4356 493 Size: 3863 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6565 SphI G_CATG'C Cuts at: 1132 3923 1132 Size: 2791 3774 SspI AAT'ATT Cuts at: 5 52 4489 5 Size: 47 4437 2081 Fragments arranged by size: 4437 2081 47 StuI AGG'CCT Cuts at: 1517 2991 1517 Size: 1474 5091 StyI C'CwwG_G Cuts at: 513 1065 2841 4004 513 Size: 552 1776 1163 3074 Fragments arranged by size: 3074 1776 1163 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2299 3061 3365 4592 4609 4762 4947 Size: 1255 719 762 304 1227 17 153 185 Cuts at: 4947 5392 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1227 762 719 445 304 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2595 3537 3735 Size: 274 80 33 51 1443 618 942 198 Cuts at: 3735 4811 6523 96 Size: 1076 1712 138 Fragments arranged by size: 1712 1443 1076 942 618 274 198 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2375 2407 2979 3011 6525 6557 2375 Size: 32 572 32 3514 32 2383 Fragments arranged by size: 3514 2383 572 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6565 XbaI T'CTAG_A Cuts at: 3901 3901 Size: 6565 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2113 2645 3466 2113 Size: 532 821 5212 Fragments arranged by size: 5212 821 532 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6565 XmaI C'CCGG_G Cuts at: 1870 3890 1870 Size: 2020 4545 XmnI GAAnn'nnTTC Cuts at: 2065 2757 4694 2065 Size: 692 1937 3936 Fragments arranged by size: 3936 1937 692 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4373 276 Size: 53 83 186 936 2839 2468 Fragments arranged by size: 2839 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI