################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BclI BfaI BfrI BlnI BpuAI BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BstBI BstEII BstPI ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MaeI MamI MfeI MluI MluNI MroI MscI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tsp509I Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AccI 427 GTATAC GT^[AC][GT]AC AccII 919 CGCG CG^CG AcyI 922 GGCGCC G[GA]^CG[CT]C Alw44I 547 GTGCAC G^TGCAC AlwI 364 GATCC GGATC(4/5) AlwNI 398 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 316 CCTGAGG CC^T[ACGT]AGG ApaLI 547 GTGCAC G^TGCAC BanI 922 GGCGCC G^G[CT][GA]CC BbeI 922 GGCGCC GGCGC^C BcgI 275 GCATTGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 390 GTATCC GTATCC(6/5) BglI 1047 GCCTCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 20 AGATCT A^GATCT BlpI 102 GCTGAGC GC^T[ACGT]AGC Bpu1102I 102 GCTGAGC GC^T[ACGT]AGC BsaHI 922 GGCGCC G[GA]^CG[CT]C BsaI 782 GAGACC GGTCTC(1/5) BsmBI 256 GAGACG CGTCTC(1/5) BsrFI 1073 GCCGGC [GA]^CCGG[CT] BssSI 226 CACGAG CACGAG(-5/-1) Bst1107I 427 GTATAC GTA^TAC BstAPI 397 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 919 CGCG CG^CG BstXI 873 CCAGCCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 20 AGATCT [GA]^GATC[CT] BstZ17I 427 GTATAC GTA^TAC Bsu36I 316 CCTGAGG CC^T[ACGT]AGG CelII 102 GCTGAGC GC^T[ACGT]AGC Cfr10I 1073 GCCGGC [GA]^CCGG[CT] DsaI 918 CCGCGG C^C[GA][CT]GG EaeI 1085 TGGCCG [CT]^GGCC[GA] Eco81I 316 CCTGAGG CC^T[ACGT]AGG EcoNI 894 CCTGGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 1082 CCTTGG C^C[AT][AT]GG HgaI 258 GACGC GACGC(5/10) Hin1I 922 GGCGCC G[GA]^CG[CT]C HinfI 45 GAATC G^A[ACGT]TC KasI 922 GGCGCC G^GCGCC KspI 918 CCGCGG CCGC^GG MaeII 58 ACGT A^CGT MflI 20 AGATCT [GA]^GATC[CT] MslI 177 CACGGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 918 CCGCGG C[AC]G^C[GT]G MvnI 919 CGCG CG^CG NaeI 1073 GCCGGC GCC^GGC NarI 922 GGCGCC GG^CGCC NgoMIV 1073 GCCGGC G^CCGGC NlaIII 183 CATG CATG^ PflMI 766 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 906 GGGTCCC [GA]G^G[AT]CC[CT] SacII 918 CCGCGG CCGC^GG SfoI 922 GGCGCC GGC^GCC SmaI 979 CCCGGG CCC^GGG StyI 1082 CCTTGG C^C[AT][AT]GG TfiI 45 GAATC G^A[AT]TC Van91I 766 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 20 AGATCT [GA]^GATC[CT] XmaCI 979 CCCGGG C^CCGGG XmaI 979 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AspHI 109 GTGCTC G[AT]GC[AT]^C 547 GTGCAC BpmI 672 CTCCAG CTGGAG(16/14) 298 CTGGAG BsiHKAI 109 GTGCTC G[AT]GC[AT]^C 547 GTGCAC BsmAI 256 GAGAC GTCTC(1/5) 782 GAGAC DpnII 21 GATC ^GATC 364 GATC EarI 160 CTCTTC CTCTTC(1/4) 556 CTCTTC Ksp632I 160 CTCTTC CTCTTC(1/4) 556 CTCTTC MboI 21 GATC ^GATC 364 GATC NdeII 21 GATC ^GATC 364 GATC PaeR7I 253 CTCGAG C^TCGAG 840 CTCGAG PstI 395 CTGCAG CTGCA^G 694 CTGCAG Sau3AI 21 GATC ^GATC 364 GATC SfaNI 29 GCATC GCATC(5/9) 950 GCATC SmlI 253 CTCGAG C^T[CT][GA]AG 840 CTCGAG StuI 92 AGGCCT AGG^CCT 540 AGGCCT XcmI 173 CCAGCACGGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 413 CCAGCCCAGATGTGG XhoI 253 CTCGAG C^TCGAG 840 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AciI AfaI AluI ApaI AvaI AvaII BanII BbvI BcnI BmyI BsaJI BseRI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeIII MboII MnlI MspI MvaI MwoI NciI NlaIV RsaI Sau96I ScrFI SfcI TaqI TseI Tsp45I TspRI TthHB8I