(Circular) (Six-base) MAPSORT of: pFN21AE1199.seq Check: 2813 from: 1 to: 5809 pFN21AE1199 5809 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2047 2047 Size: 5809 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3619 278 Size: 53 83 186 936 2083 2468 Fragments arranged by size: 2468 2083 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2433 3152 1937 Size: 496 719 4594 Fragments arranged by size: 4594 719 496 Acc65I G'GTAC_C Cuts at: 3130 3130 Size: 5809 AclI AA'CG_TT Cuts at: 1563 3936 4309 1563 Size: 2373 373 3063 Fragments arranged by size: 3063 2373 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2225 2288 3872 5256 5524 1632 Size: 593 63 1584 1384 268 1917 Fragments arranged by size: 1917 1584 1384 593 268 63 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5612 AflIII A'CryG_T Cuts at: 1203 4708 5685 1203 Size: 3505 977 1327 Fragments arranged by size: 3505 1327 977 AgeI A'CCGG_T Cuts at: 4640 4640 Size: 5809 AhdI GACnn_n'nnGTC Cuts at: 4538 4538 Size: 5809 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5809 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5809 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2696 2728 2696 Size: 32 5777 AlwNI CAG_nnn'CTG Cuts at: 2408 3552 5124 2408 Size: 1144 1572 3093 Fragments arranged by size: 3093 1572 1144 ApaI G_GGCC'C Cuts at: 2541 2658 2862 2993 3069 2541 Size: 117 204 131 76 5281 Fragments arranged by size: 5281 204 131 117 76 ApaLI G'TGCA_C Cuts at: 2552 3866 5022 2552 Size: 1314 1156 3339 Fragments arranged by size: 3339 1314 1156 ApoI r'AATT_y Cuts at: 1493 3118 3344 3496 5605 1493 Size: 1625 226 152 2109 1697 Fragments arranged by size: 2109 1697 1625 226 152 AseI AT'TA_AT Cuts at: 160 1032 4363 160 Size: 872 3331 1606 Fragments arranged by size: 3331 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5809 AvaI C'yCGr_G Cuts at: 1870 1943 2258 2658 2845 2984 3105 3134 1870 Size: 73 315 400 187 139 121 29 4545 Fragments arranged by size: 4545 400 315 187 139 121 73 29 BamHI G'GATC_C Cuts at: 3139 3506 3139 Size: 367 5442 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2927 3130 4585 618 Size: 325 217 627 1140 203 1455 1842 Fragments arranged by size: 1842 1455 1140 627 325 217 203 BanII G_rGCy'C Cuts at: 1391 2229 2541 2658 2862 2993 3069 3128 1391 Size: 838 312 117 204 131 76 59 4072 Fragments arranged by size: 4072 838 312 204 131 117 76 59 BbeI G_GCGC'C Cuts at: 2931 2931 Size: 5809 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4838 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2269 2303 4000 4034 1903 Size: 34 332 34 1697 34 3678 Fragments arranged by size: 3678 1697 332 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2406 3701 4911 2406 Size: 1295 1210 3304 Fragments arranged by size: 3304 1295 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5809 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3058 4420 136 Size: 107 122 71 631 605 1386 1362 1525 Fragments arranged by size: 1525 1386 1362 631 605 122 107 71 BglII A'GATC_T Cuts at: 2025 5804 2025 Size: 3779 2030 BlpI GC'TnA_GC Cuts at: 2108 3226 2108 Size: 1118 4691 Bme1580I G_kGCm'C Cuts at: 1790 2541 2556 2658 2862 2993 3069 3870 5026 Size: 751 15 102 204 131 76 801 1156 Cuts at: 5026 1790 Size: 2573 Fragments arranged by size: 2573 1156 801 751 204 131 102 76 15 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5809 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2460 2747 2877 Size: 416 53 83 399 24 1043 287 130 Cuts at: 2877 2942 3540 4498 442 Size: 65 598 958 1753 Fragments arranged by size: 1753 1043 958 598 416 399 287 130 83 65 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5809 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2989 2989 Size: 5809 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2324 2662 4469 1239 Size: 1085 338 1807 2579 Fragments arranged by size: 2579 1807 1085 338 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3934 4799 5097 3934 Size: 865 298 4646 Fragments arranged by size: 4646 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2781 4472 882 Size: 617 1282 1691 2219 Fragments arranged by size: 2219 1691 1282 617 BsaAI yAC'GTr Cuts at: 493 1204 3600 493 Size: 711 2396 2702 Fragments arranged by size: 2702 2396 711 BsaBI GATnn'nnATC Cuts at: 5803 5803 Size: 5809 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2928 3616 3998 4633 Size: 53 83 186 936 1395 688 382 635 Cuts at: 4633 275 Size: 1451 Fragments arranged by size: 1451 1395 936 688 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4242 4640 4914 5061 5472 4242 Size: 398 274 147 411 4579 Fragments arranged by size: 4579 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2696 2707 2726 2737 1178 Size: 30 165 30 1293 11 19 11 4250 Fragments arranged by size: 4250 1293 165 30 30 19 11 11 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2339 2921 3020 1186 Size: 1153 582 99 3975 Fragments arranged by size: 3975 1153 582 99 BseYI C'CCAG_C Cuts at: 2417 2651 2877 5012 5508 2417 Size: 234 226 2135 496 2718 Fragments arranged by size: 2718 2135 496 234 226 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5236 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3259 4020 5048 1540 Size: 275 186 1258 761 1028 2301 Fragments arranged by size: 2301 1258 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2118 2556 3870 3955 5026 2118 Size: 438 1314 85 1071 2901 Fragments arranged by size: 2901 1314 1071 438 85 BsmI GAATG_Cn' Cuts at: 3320 3413 3320 Size: 93 5716 BsmBI CGTCTCn'nnnn_ Cuts at: 2255 2255 Size: 5809 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2229 2541 2556 2658 2862 2993 Size: 399 328 111 312 15 102 204 131 Cuts at: 2993 3069 3128 3870 3955 5026 1391 Size: 76 59 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 328 312 204 131 111 102 85 76 59 15 BspEI T'CCGG_A Cuts at: 5472 5472 Size: 5809 BspHI T'CATG_A Cuts at: 3698 5428 3698 Size: 1730 4079 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2047 2616 2643 3164 844 Size: 1203 569 27 521 3489 Fragments arranged by size: 3489 1203 569 521 27 BsrBI CCG'CTC Cuts at: 1394 1823 3696 1394 Size: 429 1873 3507 Fragments arranged by size: 3507 1873 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4304 4478 65 Size: 1183 3056 174 1396 Fragments arranged by size: 3056 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3078 4453 4640 1700 Size: 110 1268 1375 187 2869 Fragments arranged by size: 2869 1375 1268 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5809 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5809 BssSI C'ACGA_G Cuts at: 2231 3869 4881 2231 Size: 1638 1012 3159 Fragments arranged by size: 3159 1638 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2408 794 Size: 1614 4195 BstBI TT'CG_AA Cuts at: 4613 4613 Size: 5809 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5809 BstXI CCAn_nnnn'nTGG Cuts at: 2885 2885 Size: 5809 BstYI r'GATC_y Cuts at: 1972 2025 3139 3506 3891 3908 5360 5446 5458 Size: 53 1114 367 385 17 1452 86 12 Cuts at: 5458 5804 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1114 385 367 346 86 53 17 12 BstZ17I GTA'TAC Cuts at: 2434 2434 Size: 5809 Bsu36I CC'TnA_GG Cuts at: 2322 2322 Size: 5809 BtgI C'CryG_G Cuts at: 513 1065 2004 2923 513 Size: 552 939 919 3399 Fragments arranged by size: 3399 939 919 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5070 Fragments arranged by size: 5070 683 56 BtsI GCAGTG_nn' Cuts at: 2483 2715 3325 4119 4139 2483 Size: 232 610 794 20 4153 Fragments arranged by size: 4153 794 610 232 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5809 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5774 DraI TTT'AAA Cuts at: 3113 3113 Size: 5809 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4816 809 Size: 507 3500 1802 Fragments arranged by size: 3500 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3090 3256 4145 Size: 54 1425 50 275 18 1260 166 889 Cuts at: 4145 5774 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1260 889 275 166 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3256 1537 Size: 275 1444 4090 Fragments arranged by size: 4090 1444 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2171 2567 3739 1353 Size: 818 396 1172 3423 Fragments arranged by size: 3423 1172 818 396 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2638 4396 4770 4916 387 Size: 1064 138 1049 1758 374 146 1280 Fragments arranged by size: 1758 1280 1064 1049 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2225 2288 2324 2662 3872 4469 5256 Size: 393 593 63 36 338 1210 597 787 Cuts at: 5256 5524 1239 Size: 268 1524 Fragments arranged by size: 1524 1210 787 597 593 393 338 268 63 36 EcoNI CCTnn'n_nnAGG Cuts at: 2903 2903 Size: 5809 EcoO109I rG'GnC_Cy Cuts at: 2318 2439 2538 2655 2765 2859 2912 2989 2990 Size: 121 99 117 110 94 53 77 1 Cuts at: 2990 2318 Size: 5137 Fragments arranged by size: 5137 121 117 110 99 94 77 53 1 EcoRI G'AATT_C Cuts at: 3118 3496 3118 Size: 378 5431 FspI TGC'GCA Cuts at: 4315 4315 Size: 5809 HaeII r_GCGC'y Cuts at: 1678 2157 2931 2971 4956 1678 Size: 479 774 40 1985 2531 Fragments arranged by size: 2531 1985 774 479 40 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4450 4482 4524 4556 1373 Size: 32 135 32 2878 32 42 32 2626 Fragments arranged by size: 2878 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3153 3399 669 Size: 988 281 1215 246 3079 Fragments arranged by size: 3079 1215 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5809 HpaI GTT'AAC Cuts at: 3399 3399 Size: 5809 KasI G'GCGC_C Cuts at: 2927 2927 Size: 5809 KpnI G_GTAC'C Cuts at: 3134 3134 Size: 5809 MfeI C'AATT_G Cuts at: 3408 3408 Size: 5809 MluI A'CGCG_T Cuts at: 5685 5685 Size: 5809 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2539 4923 5107 1305 Size: 1234 2384 184 2007 Fragments arranged by size: 2384 2007 1234 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3987 Fragments arranged by size: 3987 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2186 3767 4126 4285 518 Size: 648 1020 1581 359 159 2042 Fragments arranged by size: 2042 1581 1020 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2925 3225 3902 5050 5295 1606 Size: 1319 300 677 1148 245 2120 Fragments arranged by size: 2120 1319 1148 677 300 245 NaeI GCC'GGC Cuts at: 3080 3080 Size: 5809 NarI GG'CG_CC Cuts at: 2928 2928 Size: 5809 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4318 Fragments arranged by size: 4318 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5809 NgoMIV G'CCGG_C Cuts at: 3078 3078 Size: 5809 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5809 NotI GC'GGCC_GC Cuts at: 3256 3256 Size: 5809 NspI r_CATG'y Cuts at: 1132 1229 3167 4712 1132 Size: 97 1938 1545 2229 Fragments arranged by size: 2229 1938 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5809 PciI A'CATG_T Cuts at: 4708 4708 Size: 5809 PflMI CCAn_nnn'nTGG Cuts at: 1108 2777 1108 Size: 1669 4140 PfoI T'CCnGG_A Cuts at: 2037 2725 2037 Size: 688 5121 PmeI GTTT'AAAC Cuts at: 3113 3113 Size: 5809 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2696 2728 3878 3910 5391 5423 2696 Size: 32 1150 32 1481 32 3082 Fragments arranged by size: 3082 1481 1150 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2912 2912 Size: 5809 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5809 PsiI TTA'TAA Cuts at: 3379 3379 Size: 5809 PspOMI G'GGCC_C Cuts at: 2537 2654 2858 2989 3065 2537 Size: 117 204 131 76 5281 Fragments arranged by size: 5281 204 131 117 76 PspXI vC'TCGA_Gb Cuts at: 2845 2845 Size: 5809 PstI C_TGCA'G Cuts at: 830 2404 2703 3161 830 Size: 1574 299 458 3478 Fragments arranged by size: 3478 1574 458 299 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5809 SacII CC_GC'GG Cuts at: 2926 2926 Size: 5809 SalI G'TCGA_C Cuts at: 1936 3151 1936 Size: 1215 4594 SanDI GG'GwC_CC Cuts at: 2912 2912 Size: 5809 SbfI CC_TGCA'GG Cuts at: 3161 3161 Size: 5809 ScaI AGT'ACT Cuts at: 4057 5769 4057 Size: 1712 4097 SfcI C'TryA_G Cuts at: 826 1045 2400 2689 2699 3157 4292 4973 5164 Size: 219 1355 289 10 458 1135 681 191 Cuts at: 5164 826 Size: 1471 Fragments arranged by size: 1471 1355 1135 681 458 289 219 191 10 SfoI GGC'GCC Cuts at: 2929 2929 Size: 5809 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5809 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5809 SmaI CCC'GGG Cuts at: 1872 2986 3107 3136 1872 Size: 1114 121 29 4545 Fragments arranged by size: 4545 1114 121 29 SmlI C'TyrA_G Cuts at: 820 1017 1943 2258 2845 3913 4814 5076 820 Size: 197 926 315 587 1068 901 262 1553 Fragments arranged by size: 1553 1068 926 901 587 315 262 197 SnaBI TAC'GTA Cuts at: 493 3600 493 Size: 3107 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5809 SphI G_CATG'C Cuts at: 1132 3167 1132 Size: 2035 3774 SspI AAT'ATT Cuts at: 5 52 3733 5 Size: 47 3681 2081 Fragments arranged by size: 3681 2081 47 StuI AGG'CCT Cuts at: 1517 2099 2547 1517 Size: 582 448 4779 Fragments arranged by size: 4779 582 448 StyI C'CwwG_G Cuts at: 513 1065 2004 3087 3248 513 Size: 552 939 1083 161 3074 Fragments arranged by size: 3074 1083 939 552 161 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3836 3853 4006 4191 4636 325 Size: 1255 2256 17 153 185 445 1498 Fragments arranged by size: 2256 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2208 2217 4055 Size: 274 80 33 51 1443 231 9 1838 Cuts at: 4055 5767 96 Size: 1712 138 Fragments arranged by size: 1838 1712 1443 274 231 138 80 51 33 9 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2707 2739 5769 5801 2707 Size: 32 3030 32 2715 Fragments arranged by size: 3030 2715 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5809 XbaI T'CTAG_A Cuts at: 3145 3145 Size: 5809 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2185 2425 2185 Size: 240 5569 XhoI C'TCGA_G Cuts at: 1943 2258 2845 1943 Size: 315 587 4907 Fragments arranged by size: 4907 587 315 XmaI C'CCGG_G Cuts at: 1870 2984 3105 3134 1870 Size: 1114 121 29 4545 Fragments arranged by size: 4545 1114 121 29 XmnI GAAnn'nnTTC Cuts at: 3938 3938 Size: 5809 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3617 276 Size: 53 83 186 936 2083 2468 Fragments arranged by size: 2468 2083 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PsrI PvuII RsrII SacI SapI SexAI SfiI SrfI SwaI