(Linear) (Six-base) MAPSORT of: KIEE1199.seq Check: 9241 from: 1 to: 1104 KIEE1199.orf With 182 enzymes: SgfI * July 15, 2010 18:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 42 1104 Size: 42 1062 AccI GT'mk_AC Cuts at: 0 428 1104 Size: 428 676 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 220 283 1104 Size: 220 63 821 Fragments arranged by size: 821 220 63 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 691 723 1104 Size: 691 32 381 Fragments arranged by size: 691 381 32 AlwNI CAG_nnn'CTG Cuts at: 0 403 1104 Size: 403 701 ApaI G_GGCC'C Cuts at: 0 536 653 857 988 1064 1104 Size: 536 117 204 131 76 40 Fragments arranged by size: 536 204 131 117 76 40 ApaLI G'TGCA_C Cuts at: 0 547 1104 Size: 547 557 AvaI C'yCGr_G Cuts at: 0 253 653 840 979 1104 Size: 253 400 187 139 125 Fragments arranged by size: 400 253 187 139 125 BanI G'GyrC_C Cuts at: 0 922 1104 Size: 922 182 BanII G_rGCy'C Cuts at: 0 224 536 653 857 988 1064 1104 Size: 224 312 117 204 131 76 40 Fragments arranged by size: 312 224 204 131 117 76 40 BbeI G_GCGC'C Cuts at: 0 926 1104 Size: 926 178 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 264 298 1104 Size: 264 34 806 Fragments arranged by size: 806 264 34 BciVI GTATCCnnnnn_n' Cuts at: 0 401 1104 Size: 401 703 BglI GCCn_nnn'nGGC Cuts at: 0 1053 1104 Size: 1053 51 BglII A'GATC_T Cuts at: 0 20 1104 Size: 20 1084 BlpI GC'TnA_GC Cuts at: 0 103 1104 Size: 103 1001 Bme1580I G_kGCm'C Cuts at: 0 536 551 653 857 988 1064 1104 Size: 536 15 102 204 131 76 40 Fragments arranged by size: 536 204 131 102 76 40 15 BmrI ACTGGGnnnn_n' Cuts at: 0 455 742 872 937 1104 Size: 455 287 130 65 167 Fragments arranged by size: 455 287 167 130 65 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 984 1104 Size: 984 120 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 319 657 1104 Size: 319 338 447 Fragments arranged by size: 447 338 319 BsaI GGTCTCn'nnnn_ Cuts at: 0 776 1104 Size: 776 328 BsaHI Gr'CG_yC Cuts at: 0 923 1104 Size: 923 181 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 691 702 721 732 1104 Size: 691 11 19 11 372 Fragments arranged by size: 691 372 19 11 11 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 334 916 1015 1104 Size: 334 582 99 89 Fragments arranged by size: 582 334 99 89 BseYI C'CCAG_C Cuts at: 0 412 646 872 1104 Size: 412 234 226 232 Fragments arranged by size: 412 234 232 226 BsiHKAI G_wGCw'C Cuts at: 0 113 551 1104 Size: 113 438 553 Fragments arranged by size: 553 438 113 BsmBI CGTCTCn'nnnn_ Cuts at: 0 250 1104 Size: 250 854 Bsp1286I G_dGCh'C Cuts at: 0 113 224 536 551 653 857 988 1064 Size: 113 111 312 15 102 204 131 76 Cuts at: 1064 1104 Size: 40 Fragments arranged by size: 312 204 131 113 111 102 76 40 15 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 42 611 638 1104 Size: 42 569 27 466 Fragments arranged by size: 569 466 42 27 BsrFI r'CCGG_y Cuts at: 0 1073 1104 Size: 1073 31 BssSI C'ACGA_G Cuts at: 0 226 1104 Size: 226 878 BstAPI GCAn_nnn'nTGC Cuts at: 0 403 1104 Size: 403 701 BstXI CCAn_nnnn'nTGG Cuts at: 0 880 1104 Size: 880 224 BstYI r'GATC_y Cuts at: 0 20 1104 Size: 20 1084 BstZ17I GTA'TAC Cuts at: 0 429 1104 Size: 429 675 Bsu36I CC'TnA_GG Cuts at: 0 317 1104 Size: 317 787 BtgI C'CryG_G Cuts at: 0 918 1104 Size: 918 186 BtsI GCAGTG_nn' Cuts at: 0 478 710 1104 Size: 478 232 394 Fragments arranged by size: 478 394 232 EaeI y'GGCC_r Cuts at: 0 1085 1104 Size: 1085 19 EarI CTCTTCn'nnn_ Cuts at: 0 166 562 1104 Size: 166 396 542 Fragments arranged by size: 542 396 166 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 633 1104 Size: 633 471 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 220 283 319 657 1104 Size: 220 63 36 338 447 Fragments arranged by size: 447 338 220 63 36 EcoNI CCTnn'n_nnAGG Cuts at: 0 898 1104 Size: 898 206 EcoO109I rG'GnC_Cy Cuts at: 0 313 434 533 650 760 854 907 984 Size: 313 121 99 117 110 94 53 77 Cuts at: 984 985 1104 Size: 1 119 Fragments arranged by size: 313 121 119 117 110 99 94 77 53 1 HaeII r_GCGC'y Cuts at: 0 152 926 966 1104 Size: 152 774 40 138 Fragments arranged by size: 774 152 138 40 KasI G'GCGC_C Cuts at: 0 922 1104 Size: 922 182 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 534 1104 Size: 534 570 MslI CAynn'nnrTG Cuts at: 0 181 1104 Size: 181 923 MspA1I CmG'CkG Cuts at: 0 920 1104 Size: 920 184 NaeI GCC'GGC Cuts at: 0 1075 1104 Size: 1075 29 NarI GG'CG_CC Cuts at: 0 923 1104 Size: 923 181 NgoMIV G'CCGG_C Cuts at: 0 1073 1104 Size: 1073 31 PflMI CCAn_nnn'nTGG Cuts at: 0 772 1104 Size: 772 332 PfoI T'CCnGG_A Cuts at: 0 32 720 1104 Size: 32 688 384 Fragments arranged by size: 688 384 32 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 691 723 1104 Size: 691 32 381 Fragments arranged by size: 691 381 32 PpuMI rG'GwC_Cy Cuts at: 0 907 1104 Size: 907 197 PspOMI G'GGCC_C Cuts at: 0 532 649 853 984 1060 1104 Size: 532 117 204 131 76 44 Fragments arranged by size: 532 204 131 117 76 44 PspXI vC'TCGA_Gb Cuts at: 0 840 1104 Size: 840 264 PstI C_TGCA'G Cuts at: 0 399 698 1104 Size: 399 299 406 Fragments arranged by size: 406 399 299 SacII CC_GC'GG Cuts at: 0 921 1104 Size: 921 183 SanDI GG'GwC_CC Cuts at: 0 907 1104 Size: 907 197 SfcI C'TryA_G Cuts at: 0 395 684 694 1104 Size: 395 289 10 410 Fragments arranged by size: 410 395 289 10 SfoI GGC'GCC Cuts at: 0 924 1104 Size: 924 180 SmaI CCC'GGG Cuts at: 0 981 1104 Size: 981 123 SmlI C'TyrA_G Cuts at: 0 253 840 1104 Size: 253 587 264 Fragments arranged by size: 587 264 253 StuI AGG'CCT Cuts at: 0 94 542 1104 Size: 94 448 562 Fragments arranged by size: 562 448 94 StyI C'CwwG_G Cuts at: 0 1082 1104 Size: 1082 22 TatI w'GTAC_w Cuts at: 0 203 212 1104 Size: 203 9 892 Fragments arranged by size: 892 203 9 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 702 734 1104 Size: 702 32 370 Fragments arranged by size: 702 370 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 180 420 1104 Size: 180 240 684 Fragments arranged by size: 684 240 180 XhoI C'TCGA_G Cuts at: 0 253 840 1104 Size: 253 587 264 Fragments arranged by size: 587 264 253 XmaI C'CCGG_G Cuts at: 0 979 1104 Size: 979 125 Enzymes that do cut: AarI AccI AcuI AloI AlwNI ApaI ApaLI AvaI BanI BanII BbeI BcgI BciVI BglI BglII BlpI Bme1580I BmrI BplI BpmI BsaI BsaHI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BssSI BstAPI BstXI BstYI BstZ17I Bsu36I BtgI BtsI EaeI EarI EciI Eco57MI EcoNI EcoO109I HaeII KasI MmeI MslI MspA1I NaeI NarI NgoMIV PflMI PfoI PpiI PpuMI PspOMI PspXI PstI SacII SanDI SfcI SfoI SmaI SmlI StuI StyI TatI TstI XcmI XhoI XmaI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BclI BfrBI BmgBI BmtI Bpu10I BpuEI BsaAI BsaBI BsaWI BsgI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI BssHII BstBI BstEII BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MscI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PshAI PsiI PsrI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I XbaI XmnI ZraI