Restriction map for
pFN21AE1799
(Linear) (Six-base) MAPSORT of: KIEE1799.seq Check: 4661 from: 1 to: 2529
KIEE1799.orf
With 182 enzymes: SgfI *
July 15, 2010 18:47 ..
AccI GT'mk_AC
Cuts at: 0 634 2529
Size: 634 1895
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1073 1988 2529
Size: 1073 915 541
Fragments arranged by size:
1073 915 541
AfeI AGC'GCT
Cuts at: 0 664 2110 2529
Size: 664 1446 419
Fragments arranged by size:
1446 664 419
AflII C'TTAA_G
Cuts at: 0 2029 2529
Size: 2029 500
AhdI GACnn_n'nnGTC
Cuts at: 0 1415 2529
Size: 1415 1114
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2403 2529
Size: 2403 126
AlwNI CAG_nnn'CTG
Cuts at: 0 157 1225 2529
Size: 157 1068 1304
Fragments arranged by size:
1304 1068 157
ApoI r'AATT_y
Cuts at: 0 595 802 1039 1351 1705 2127 2529
Size: 595 207 237 312 354 422 402
Fragments arranged by size:
595 422 402 354 312 237 207
AseI AT'TA_AT
Cuts at: 0 788 883 2529
Size: 788 95 1646
Fragments arranged by size:
1646 788 95
BanI G'GyrC_C
Cuts at: 0 2320 2529
Size: 2320 209
BanII G_rGCy'C
Cuts at: 0 1332 1531 2529
Size: 1332 199 998
Fragments arranged by size:
1332 998 199
BbsI GAAGACnn'nnnn_
Cuts at: 0 338 353 2529
Size: 338 15 2176
Fragments arranged by size:
2176 338 15
BbvCI CC'TCA_GC
Cuts at: 0 2301 2529
Size: 2301 228
BciVI GTATCCnnnnn_n'
Cuts at: 0 1798 2201 2529
Size: 1798 403 328
Fragments arranged by size:
1798 403 328
BfrBI ATG'CAT
Cuts at: 0 1872 2235 2529
Size: 1872 363 294
Fragments arranged by size:
1872 363 294
BglII A'GATC_T
Cuts at: 0 676 2529
Size: 676 1853
BlpI GC'TnA_GC
Cuts at: 0 818 2529
Size: 818 1711
BmrI ACTGGGnnnn_n'
Cuts at: 0 1577 1819 2529
Size: 1577 242 710
Fragments arranged by size:
1577 710 242
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 1669 2007 2529
Size: 56 1613 338 522
Fragments arranged by size:
1613 522 338 56
Bpu10I CC'TnA_GC
Cuts at: 0 2301 2529
Size: 2301 228
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1226 1677 2171 2529
Size: 1226 451 494 358
Fragments arranged by size:
1226 494 451 358
BsaI GGTCTCn'nnnn_
Cuts at: 0 366 2529
Size: 366 2163
BsaBI GATnn'nnATC
Cuts at: 0 681 2529
Size: 681 1848
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1457 1487 1518 1548 2529
Size: 1457 30 31 30 981
Fragments arranged by size:
1457 981 31 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 57 259 389 944 1130 1657 2529
Size: 57 202 130 555 186 527 872
Fragments arranged by size:
872 555 527 202 186 130 57
BseYI C'CCAG_C
Cuts at: 0 2111 2529
Size: 2111 418
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1163 2529
Size: 1163 1366
BsiHKAI G_wGCw'C
Cuts at: 0 1655 2187 2529
Size: 1655 532 342
Fragments arranged by size:
1655 532 342
BsmI GAATG_Cn'
Cuts at: 0 118 1570 2058 2529
Size: 118 1452 488 471
Fragments arranged by size:
1452 488 471 118
Bsp1286I G_dGCh'C
Cuts at: 0 1332 1531 1655 2187 2529
Size: 1332 199 124 532 342
Fragments arranged by size:
1332 532 342 199 124
BsrDI GCAATG_nn'
Cuts at: 0 123 315 1365 1425 1674 1803 2529
Size: 123 192 1050 60 249 129 726
Fragments arranged by size:
1050 726 249 192 129 123 60
BsrFI r'CCGG_y
Cuts at: 0 1370 2529
Size: 1370 1159
BsrGI T'GTAC_A
Cuts at: 0 1787 2064 2529
Size: 1787 277 465
Fragments arranged by size:
1787 465 277
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1631 2529
Size: 1631 898
BstYI r'GATC_y
Cuts at: 0 676 1597 2529
Size: 676 921 932
Fragments arranged by size:
932 921 676
Bsu36I CC'TnA_GG
Cuts at: 0 1017 2529
Size: 1017 1512
BtsI GCAGTG_nn'
Cuts at: 0 2099 2529
Size: 2099 430
ClaI AT'CG_AT
Cuts at: 0 389 2529
Size: 389 2140
DraI TTT'AAA
Cuts at: 0 1079 2529
Size: 1079 1450
EaeI y'GGCC_r
Cuts at: 0 444 1829 2529
Size: 444 1385 700
Fragments arranged by size:
1385 700 444
EarI CTCTTCn'nnn_
Cuts at: 0 1331 2529
Size: 1331 1198
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 1073 1669 1988 2007 2529
Size: 56 1017 596 319 19 522
Fragments arranged by size:
1017 596 522 319 56 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 227 2529
Size: 227 2302
EcoO109I rG'GnC_Cy
Cuts at: 0 1409 1999 2529
Size: 1409 590 530
Fragments arranged by size:
1409 590 530
EcoRI G'AATT_C
Cuts at: 0 1705 2127 2529
Size: 1705 422 402
Fragments arranged by size:
1705 422 402
EcoRV GAT'ATC
Cuts at: 0 387 2529
Size: 387 2142
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 392 684 2529
Size: 392 292 1845
Fragments arranged by size:
1845 392 292
HaeII r_GCGC'y
Cuts at: 0 666 2112 2465 2529
Size: 666 1446 353 64
Fragments arranged by size:
1446 666 353 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 123 155 891 923 1401 1433 2199 2231
Size: 123 32 736 32 478 32 766 32
Cuts at: 2231 2529
Size: 298
Fragments arranged by size:
766 736 478 298 123 32 32 32
32
HincII GTy'rAC
Cuts at: 0 78 635 2084 2529
Size: 78 557 1449 445
Fragments arranged by size:
1449 557 445 78
HindIII A'AGCT_T
Cuts at: 0 12 2312 2529
Size: 12 2300 217
Fragments arranged by size:
2300 217 12
HpaI GTT'AAC
Cuts at: 0 2084 2529
Size: 2084 445
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 262 637 1486 1587 1723 2529
Size: 171 91 375 849 101 136 806
Fragments arranged by size:
849 806 375 171 136 101 91
MscI TGG'CCA
Cuts at: 0 446 1831 2529
Size: 446 1385 698
Fragments arranged by size:
1385 698 446
MslI CAynn'nnrTG
Cuts at: 0 25 716 2385 2413 2529
Size: 25 691 1669 28 116
Fragments arranged by size:
1669 691 116 28 25
MspA1I CmG'CkG
Cuts at: 0 2060 2285 2305 2529
Size: 2060 225 20 224
Fragments arranged by size:
2060 225 224 20
NaeI GCC'GGC
Cuts at: 0 1372 2529
Size: 1372 1157
NgoMIV G'CCGG_C
Cuts at: 0 1370 2529
Size: 1370 1159
NsiI A_TGCA'T
Cuts at: 0 1874 2237 2529
Size: 1874 363 292
Fragments arranged by size:
1874 363 292
NspI r_CATG'y
Cuts at: 0 1989 2239 2529
Size: 1989 250 290
Fragments arranged by size:
1989 290 250
PflMI CCAn_nnn'nTGG
Cuts at: 0 750 2529
Size: 750 1779
PpuMI rG'GwC_Cy
Cuts at: 0 1409 1999 2529
Size: 1409 590 530
Fragments arranged by size:
1409 590 530
PshAI GACnn'nnGTC
Cuts at: 0 1361 2529
Size: 1361 1168
PstI C_TGCA'G
Cuts at: 0 2126 2529
Size: 2126 403
PvuII CAG'CTG
Cuts at: 0 2285 2305 2529
Size: 2285 20 224
Fragments arranged by size:
2285 224 20
SalI G'TCGA_C
Cuts at: 0 633 2529
Size: 633 1896
SanDI GG'GwC_CC
Cuts at: 0 1409 2529
Size: 1409 1120
ScaI AGT'ACT
Cuts at: 0 609 2529
Size: 609 1920
SfcI C'TryA_G
Cuts at: 0 528 864 2122 2529
Size: 528 336 1258 407
Fragments arranged by size:
1258 528 407 336
SmlI C'TyrA_G
Cuts at: 0 1241 1656 2029 2186 2529
Size: 1241 415 373 157 343
Fragments arranged by size:
1241 415 373 343 157
StuI AGG'CCT
Cuts at: 0 2508 2529
Size: 2508 21
StyI C'CwwG_G
Cuts at: 0 103 1539 2171 2529
Size: 103 1436 632 358
Fragments arranged by size:
1436 632 358 103
TatI w'GTAC_w
Cuts at: 0 607 1787 1902 2064 2287 2529
Size: 607 1180 115 162 223 242
Fragments arranged by size:
1180 607 242 223 162 115
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1518 1550 1811 1843 2529
Size: 1518 32 261 32 686
Fragments arranged by size:
1518 686 261 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2442 2529
Size: 2442 87
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 187 2529
Size: 187 2342
Enzymes that do cut:
AccI AcuI AfeI AflII AhdI AlfI AlwNI ApoI
AseI BanI BanII BbsI BbvCI BciVI BfrBI BglII
BlpI BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BsrDI BsrFI
BsrGI BstAPI BstYI Bsu36I BtsI ClaI DraI EaeI
EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII
Hin4I HincII HindIII HpaI MmeI MscI MslI MspA1I
NaeI NgoMIV NsiI NspI PflMI PpuMI PshAI PstI
PvuII SalI SanDI ScaI SfcI SmlI StuI StyI
TatI TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflIII AgeI AleI AloI
ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI
BbeI BcgI BclI BglI Bme1580I BmgBI BmtI BplI
BsaAI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BspHI
BspMI BsrBI BssHII BssSI BstBI BstEII BstXI BstZ17I
BtgI BtgZI CspCI DraIII DrdI EagI EciI EcoICRI
FseI FspI FspAI KasI KpnI MfeI MluI NarI
NcoI NdeI NheI NotI NruI PacI PasI PciI
PfoI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TaqII XbaI XhoI XmaI XmnI ZraI